LOCUS       sequence-217286-        9042 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  fluorescent, functional, glutamate receptor subunit.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9042)
  AUTHORS   Sheridan DL, Robert A, Cho CH, Howe JR, Hughes TE
  TITLE     Regions of alpha-amino-5-methyl-3-hydroxy-4-isoxazole propionic acid
            receptor subunits that are permissive for the insertion of green 
            fluorescent protein.
  JOURNAL   Neuroscience. 2006 Aug 25;141(2):837-49. Epub 2006 Jun 12.
  PUBMED    16765522
REFERENCE   2  (bases 1 to 9042)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Neuroscience."; date: "2006-08-25"; volume: "141(2)"; pages: 
            "837-49. Epub 2006 Jun 12"
COMMENT     Sequence Label: pR2Q358y
FEATURES             Location/Qualifiers
     source          1..9042
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        17..396
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        397..600
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     551..571
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     597..621
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        831..849
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     831..848
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     862..881
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     939..955
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     939..955
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     misc_feature    3845..3863
                     /label=Tn5 ME
                     /note="hyperactive mosaic end for Tn5 transposase 
                     recognition (Reznikoff et al., 2004)"
     CDS             3872..4582
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLEF
                     VTAAGITLGMDELYK"
     primer_bind     complement(3911..3932)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4172..4191)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4519..4540
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    4600..4618
                     /label=Tn5 ME
                     /note="hyperactive mosaic end for Tn5 transposase 
                     recognition (Reznikoff et al., 2004)"
     regulatory      5345..5354
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    5358..5492
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5395..5414)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5449..5468
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        5561..5918
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5561..5752
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5584..5604)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5769..5904
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5938..5955)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5938..5954)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5947..5969)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6167..6622
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6254..6273)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6464..6485
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6639..6658)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6758..6780
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6818..6836)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6904..7008
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7009..7077,7078..7869)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7227..7246)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8040..8628
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8529..8548
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8782..8799
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8916..8937
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8952..8957,8958..8975,8976..8982)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8990..9006
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8995..9017
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9014..9030
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9014..9030
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 taattcgagc tcgcccgaca ttgattattg actagttatt aatagtaatc aattacgggg
       61 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg
      121 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
      181 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
      241 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
      301 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
      361 cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc
      421 aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc
      481 aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc
      541 gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct
      601 cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga cctccataga
      661 agacaccggg accgatccag cctccgcggc cgggaacggt gcattggaac gcggattccc
      721 cgtgccaaga gtgacgtaag taccgcctat agagtctata ggcccacccc cttggcttcg
      781 ttagaacgcg gctacaatta atacataacc ttatgtatca tacacatacg atttaggtga
      841 cactatagaa taacatccac tttgcctttc tctccacagg tgtccactcc caggtccaac
      901 tgcacctcgg ttctatcgat tgaattcccc ggggatcctc tagaactagt ggatcccccg
      961 ggctgcagga attcggcacg aggccagtcc tcgggacttc gggagcaggg acaggacgca
     1021 aggcatcaac agccaccagc tacaactggg aaataaggat tcttctgcct tcacttcgtg
     1081 tttttagcag ctccttgcta aatatcgacc tcacaatgca gaggatctaa tttgctgagg
     1141 aaaacagtca aagaaggaag aggaagaaag ggaaacgagg ggatattttg tggatgctct
     1201 acttttcttg gaaatgcaaa agattatgca tatttctgtc ctcctttctc ctgttttatg
     1261 gggactgatt tttggtgtct cttctaacag catacagata ggggggctat ttccaagggg
     1321 cgctgatcaa gaatacagtg catttcgggt agggatggtt cagttttcca cttcggagtt
     1381 cagactgaca ccccatatcg acaatttgga ggtagccaac agtttcgcag tcaccaatgc
     1441 tttctgctcc cagttttcaa gaggagtcta cgcaattttt ggattttatg acaagaagtc
     1501 tgtaaatacc atcacatcat tctgtgggac actccatgtg tccttcatca cacctagctt
     1561 cccaacagat ggcacacatc catttgtcat ccagatgcga cctgacctca aaggagcact
     1621 ccttagcttg attgagtact accaatggga caagttcgca tacctctatg acagtgacag
     1681 aggcttatca acactgcaag ctgttctgga ttctgctgca gagaagaagt ggcaggtgac
     1741 tgctatcaat gtggggaaca tcaacaatga caagaaagat gagacctaca gatcgctctt
     1801 tcaagatctg gagttaaaaa aagaacggcg tgtaatcctg gactgtgaaa gggataaagt
     1861 aaatgacatt gtggaccagg ttattaccat tggaaaacat gttaaagggt accattatat
     1921 cattgcaaat ctgggattca ctgatgggga cctgctgaaa attcagtttg gaggagcaaa
     1981 tgtctctgga tttcagattg tagactacga tgattccctg gtgtctaaat ttatagaaag
     2041 atggtcaaca ctggaagaga aagaataccc tggagcacac acagcgacaa ttaagtatac
     2101 ttcggccctg acgtatgatg ctgtccaagt gatgactgaa gcattccgta accttcggaa
     2161 gcagaggatt gaaatatccc ggagaggaaa tgcaggggat tgtttggcca acccagctgt
     2221 gccctgggga caaggggtcg aaatagaaag ggccctcaag caggttcaag ttgaaggcct
     2281 ctctggaaat ataaagtttg accagaatgg aaaacgaata aactacacaa ttaacatcat
     2341 ggagctcaaa acaaatggac cccggaagat tgggtactgg agtgaagtgg ataaaatggt
     2401 tgtcacccta actgagctcc catcaggaaa tgacacgtct gggcttgaaa acaagactgt
     2461 ggtggtcacc acaatattgg aatctccata tgttatgatg aagaaaaatc atgaaatgct
     2521 tgaagggaat gagcgttacg agggctactg tgttgactta gctgcagaaa ttgccaaaca
     2581 ctgtgggttc aagtacaagc tgactattgt tggggatggc aagtatgggg ccagggatgc
     2641 cgacaccaaa atttggaatg gtatggttgg agagcttgtc tacgggaaag ctgacattgc
     2701 aattgctcca ttaactatca ctctcgtgag agaagaggtg attgacttct ccaagccctt
     2761 catgagtctt ggaatctcta tcatgatcaa gaagcctcag aagtccaaac caggagtgtt
     2821 ttcctttctt gatcctttag cctatgagat ctggatgtgc attgtgtttg cctacattgg
     2881 ggtcagtgta gttttattcc tggtcagcag atttagcccc tacgagtggc acactgagga
     2941 atttgaagat ggaagagaaa cacaaagtag tgaatcaact aatgaatttg ggatttttaa
     3001 tagtctctgg ttttccttgg gtgcctttat gcagcaggga tgcgatattt cgccaagatc
     3061 cctctctggg cgcattgttg gaggtgtgtg gtggttcttt accctgatca taatctcctc
     3121 ctacacggct aacttagctg ccttcctgac tgtagagagg atggtgtctc ccatcgaaag
     3181 tgctgaggat ctgtctaagc aaacagaaat tgcttatgga acattagact ctggctccac
     3241 taaagagttt ttcaggagat ctaaaatcgc agtgtttgat aaaatgtgga cttatatgag
     3301 gagtgcagag ccctctgtgt ttgtgaggac taccgcagaa ggagtagcca gagtccggaa
     3361 atccaaagga aagtatgcct acttgctgga gtccacaatg aacgagtaca tcgagcagag
     3421 gaagccttgt gacaccatga aagtgggagg aaacttggat tccaaaggct acggcatcgc
     3481 cacacctaaa ggatcctcat taggaacccc agtaaatctt gcagtattga aactcagtga
     3541 gcaaggcgtc ttagacaagc tgaaaaacaa atggtggtac gataaaggtg aatgtggagc
     3601 caaggactcg ggaagtaagg aaaagaccag tgccctcagt ctgagcaacg ttgctggagt
     3661 attctacatc cttgtcgggg gccttggttt ggcaatgctg gtggctttga ttgagttctg
     3721 ttacaagtca agggccgagg cgaaacgaat gaaggtggca aagaatccac agaatattaa
     3781 cccatcttcc tcgcagaatt cccagaattt tgcaacttat aaggaaggtt acaacgtata
     3841 tggcctgtct cttatacaca tctggcgcgc cagcaagggc gaggagctgt tcaccggggt
     3901 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg
     3961 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg
     4021 caagctgccc gtgccctggc ccaccctcgt gaccaccttc ggctacggcc tgcagtgctt
     4081 cgcccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
     4141 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
     4201 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
     4261 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta
     4321 tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat
     4381 cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
     4441 ccccgtgctg ctgcccgaca accactacct gagctaccag tccgccctga gcaaagaccc
     4501 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct
     4561 cggcatggac gagctgtaca agggtgggcg cgcccgggca gatgtgtata agagacaggt
     4621 atatggcatc gagagtgtta aaatttaggg gatgaccttg agtgatgtca tgaggagcaa
     4681 ggcaaggctg tcaattacag gaagtactgg agaaaatgga cgtgttatga ctccagaatt
     4741 tcccaaagca gtgcatgctg tcccttacgt gagtcctggc atgggaatga atgtcagtgt
     4801 gactgatctc tcgtgattga taggaacctt ctgagtgcct tacacaatgg tttccttgtg
     4861 tgtttattgt caaagtggtg agaggcatcc gatatcttga aggcttttct ttcagccaag
     4921 aattcttaac tatgtggagt tcaccttgaa ttgtaaggaa agataaatta caaacagagc
     4981 atcattttct actccgatat cagaggaagc gtggtggaca tgcacagcta acatggaaat
     5041 actatcattt aactgaagtc tttgtacaga caacaaaccc gtttccgcag ccactattgt
     5101 tagtctcttg attcataatg acttaagcac acttgacatc aactgcatca agatgtgacc
     5161 tgttttataa ataaaaagga aaaaaacatt taaaataaaa aaatattttt aggtattttc
     5221 acagacaaac tggcttttaa ataaatttgc ttccgtactg gttggttaag acaaatacaa
     5281 aaacaaaaac aaaacaaaac aaaaaccaaa aaaaaaaaaa aaaaactcga cctgcagaag
     5341 cttggccgcc atggcccaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     5401 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     5461 aactcatcaa tgtatcttat catgtctgga tcgggaatta attcggcgca gcaccatggc
     5521 ctgaaataac ctctgaaaga ggaacttggt taggtacctt ctgaggcgga aagaaccagc
     5581 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta
     5641 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
     5701 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
     5761 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     5821 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
     5881 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctgttaaca gcttggcact
     5941 ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct
     6001 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc
     6061 ttcccaacag ttgcgcagcc tgaatggcga atggcgcctg atgcggtatt ttctccttac
     6121 gcatctgtgc ggtatttcac accgcatacg tcaaagcaac catagtacgc gccctgtagc
     6181 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     6241 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     6301 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     6361 ctcgacccca aaaaacttga tttgggtgat ggttcacgta gtgggccatc gccctgatag
     6421 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     6481 actggaacaa cactcaaccc tatctcgggc tattcttttg atttataagg gattttgccg
     6541 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
     6601 aaaatattaa cgtttacaat tttatggtgc actctcagta caatctgctc tgatgccgca
     6661 tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg
     6721 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg
     6781 ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta
     6841 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat
     6901 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     6961 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     7021 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     7081 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     7141 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     7201 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     7261 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     7321 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     7381 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     7441 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     7501 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     7561 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     7621 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     7681 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     7741 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     7801 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     7861 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     7921 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     7981 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     8041 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     8101 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     8161 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     8221 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     8281 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     8341 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     8401 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     8461 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     8521 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     8581 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     8641 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     8701 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     8761 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     8821 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     8881 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
     8941 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga
     9001 taacaatttc acacaggaaa cagctatgac atgattacga at
//
