LOCUS       sequence-217290-        9890 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses 10xHis-MBP fused to AsCas12a.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9890)
  AUTHORS   Chen JS, Ma E, Harrington LB, Da Costa M, Tian X, Palefsky JM, 
            Doudna JA
  TITLE     CRISPR-Cas12a target binding unleashes indiscriminate 
            single-stranded DNase activity.
  JOURNAL   Science. 2018 Apr 27;360(6387):436-439. doi: 
            10.1126/science.aar6245. Epub 2018 Feb 15.
  PUBMED    29449511
REFERENCE   2  (bases 1 to 9890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.aar6245"; journalName: "Science"; date: 
            "2018-04-27- 27"; volume: "360"; issue: "6387"; pages: "436-439"
COMMENT     Sequence Label: pMBP-AsCas12a
FEATURES             Location/Qualifiers
     source          1..9890
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1051..1070
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     1154..1173
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1154..1172
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             1261..1283
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1303..1332
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             1342..2442
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
     primer_bind     2416..2439
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             2497..2517
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             2524..6444
                     /codon_start=1
                     /product="CRISPR-associated protein from Acidaminococcus 
                     sp. BV3L6 (Zetsche et al., 2015)"
                     /label=AsCpf1
                     /translation="MTQFEGFTNLYQVSKTLRFELIPQGKTLKHIQEQGFIEEDKARND
                     HYKELKPIIDRIYKTYADQCLQLVQLDWENLSAAIDSYRKEKTEETRNALIEEQATYRN
                     AIHDYFIGRTDNLTDAINKRHAEIYKGLFKAELFNGKVLKQLGTVTTTEHENALLRSFD
                     KFTTYFSGFYENRKNVFSAEDISTAIPHRIVQDNFPKFKENCHIFTRLITAVPSLREHF
                     ENVKKAIGIFVSTSIEEVFSFPFYNQLLTQTQIDLYNQLLGGISREAGTEKIKGLNEVL
                     NLAIQKNDETAHIIASLPHRFIPLFKQILSDRNTLSFILEEFKSDEEVIQSFCKYKTLL
                     RNENVLETAEALFNELNSIDLTHIFISHKKLETISSALCDHWDTLRNALYERRISELTG
                     KITKSAKEKVQRSLKHEDINLQEIISAAGKELSEAFKQKTSEILSHAHAALDQPLPTTL
                     KKQEEKEILKSQLDSLLGLYHLLDWFAVDESNEVDPEFSARLTGIKLEMEPSLSFYNKA
                     RNYATKKPYSVEKFKLNFQMPTLASGWDVNKEKNNGAILFVKNGLYYLGIMPKQKGRYK
                     ALSFEPTEKTSEGFDKMYYDYFPDAAKMIPKCSTQLKAVTAHFQTHTTPILLSNNFIEP
                     LEITKEIYDLNNPEKEPKKFQTAYAKKTGDQKGYREALCKWIDFTRDFLSKYTKTTSID
                     LSSLRPSSQYKDLGEYYAELNPLLYHISFQRIAEKEIMDAVETGKLYLFQIYNKDFAKG
                     HHGKPNLHTLYWTGLFSPENLAKTSIKLNGQAELFYRPKSRMKRMAHRLGEKMLNKKLK
                     DQKTPIPDTLYQELYDYVNHRLSHDLSDEARALLPNVITKEVSHEIIKDRRFTSDKFFF
                     HVPITLNYQAANSPSKFNQRVNAYLKEHPETPIIGIDRGERNLIYITVIDSTGKILEQR
                     SLNTIQQFDYQKKLDNREKERVAARQAWSVVGTIKDLKQGYLSQVIHEIVDLMIHYQAV
                     VVLENLNFGFKSKRTGIAEKAVYQQFEKMLIDKLNCLVLKDYPAEKVGGVLNPYQLTDQ
                     FTSFAKMGTQSGFLFYVPAPYTSKIDPLTGFVDPFVWKTIKNHESRKHFLEGFDFLHYD
                     VKTGDFILHFKMNRNLSFQRGLPGFMPAWDIVFEKNETQFDAKGTPFIAGKRIVPVIEN
                     HRFTGRYRDLYPANELIALLEEKGIVFRDGSNILPKLLENDDSHAIDTMVALIRSVLQM
                     RNSNAATGEDYINSPVRDLNGVCFDSRFQNPEWPMDADANGAYHIALKGQLLLNHLKES
                     KDLKLQNGISNQDWLAYIQELRN"
     primer_bind     complement(6573..6591)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      6587..6634
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(7000..7005,7006..7022,7023..7028))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(7055..7073)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7141..7245
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7246..7314,7315..8106)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7464..7483)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8277..8865
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8766..8785
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9019..9036
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    9051..9190
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(9116..9135)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9276..9298
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(9292..9483)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg
       61 gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga
      121 tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg
      181 ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga
      241 ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc
      301 gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac
      361 gatcagcggt ccaatgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg
      421 tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat
      481 gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc
      541 cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc
      601 gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa
      661 taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat
      721 gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag
      781 tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct
      841 caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag
      901 taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc
      961 caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag
     1021 cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac
     1081 cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc
     1141 gatcccgcga aattaatacg actcactata gggagaccac aacggtttcc ctctagtgcc
     1201 ggctccggag agctctttaa ttaagcggcc gccctgcagg actcgagttc tagaaataat
     1261 tttgtttaac tttaagaagg agatatacat atgaaatctt ctcaccatca ccatcaccat
     1321 caccatcacc atggttcttc tatgaaaatc gaagaaggta aactggtaat ctggattaac
     1381 ggcgataaag gctataacgg tctcgctgaa gtcggtaaga aattcgagaa agataccgga
     1441 attaaagtca ccgttgagca tccggataaa ctggaagaga aattcccaca ggttgcggca
     1501 actggcgatg gccctgacat tatcttctgg gcacacgacc gctttggtgg ctacgctcaa
     1561 tctggcctgt tggctgaaat caccccggac aaagcgttcc aggacaagct gtatccgttt
     1621 acctgggatg ccgtacgtta caacggcaag ctgattgctt acccgatcgc tgttgaagcg
     1681 ttatcgctga tttataacaa agatctgctg ccgaacccgc caaaaacctg ggaagagatc
     1741 ccggcgctgg ataaagaact gaaagcgaaa ggtaagagcg cgctgatgtt caacctgcaa
     1801 gaaccgtact tcacctggcc gctgattgct gctgacgggg gttatgcgtt caagtatgaa
     1861 aacggcaagt acgacattaa agacgtgggc gtggataacg ctggcgcgaa agcgggtctg
     1921 accttcctgg ttgacctgat taaaaacaaa cacatgaatg cagacaccga ttactccatc
     1981 gcagaagctg cctttaataa aggcgaaaca gcgatgacca tcaacggccc gtgggcatgg
     2041 tccaacatcg acaccagcaa agtgaattat ggtgtaacgg tactgccgac cttcaagggt
     2101 caaccatcca aaccgttcgt tggcgtgctg agcgcaggta ttaacgccgc cagtccgaac
     2161 aaagagctgg caaaagagtt cctcgaaaac tatctgctga ctgatgaagg tctggaagcg
     2221 gttaataaag acaaaccgct gggtgccgta gcgctgaagt cttacgagga agagttggcg
     2281 aaagatccac gtattgccgc cactatggaa aacgcccaga aaggtgaaat catgccgaac
     2341 atcccgcaga tgtccgcttt ctggtatgcc gtgcgtactg cggtgatcaa cgccgccagc
     2401 ggtcgtcaga ctgtcgatga agccctgaaa gacgcgcaga ctaattcgag ctcgaacaac
     2461 aacaacaata acaataacaa caacctcggg atcgaggaaa acctgtactt ccaatccaat
     2521 gcaatgaccc agttcgaggg gtttaccaat ctgtatcaag tgagcaagac gctgcgcttt
     2581 gaactgatcc cacagggaaa aaccttaaaa catattcaag agcagggctt tatcgaagaa
     2641 gataaggccc gtaatgacca ttacaaagag ttaaagccga ttattgatcg tatctacaag
     2701 acctatgcgg accagtgctt acaattggta cagcttgatt gggagaacct ctctgccgcc
     2761 atcgattcct atcgtaaaga aaaaactgaa gaaacgcgca acgccctgat tgaagagcag
     2821 gccacctatc gtaacgcgat tcatgactat tttattggcc gtacggacaa tctgacggac
     2881 gcgatcaaca agcgccatgc ggagatttac aaaggactgt ttaaggctga actgttcaat
     2941 ggtaaggtcc ttaaacagct tgggaccgtc acaacgacgg aacatgaaaa cgcgttatta
     3001 cgtagcttcg acaagtttac cacgtatttc tccggctttt acgaaaatcg caaaaacgtt
     3061 ttcagtgccg aggatatttc cactgctatc cctcatcgca ttgtgcaaga caacttccca
     3121 aaattcaaag aaaattgtca tatcttcacc cgcttaatca ccgctgtacc gtccctgcgt
     3181 gagcatttcg aaaacgtgaa aaaggccatt ggtatcttcg tgtctacttc gattgaggag
     3241 gtattttcct ttccattcta taatcagctg ctgacccaga cccaaattga tctgtacaac
     3301 cagctgcttg gcggtatttc tcgtgaagca ggaaccgaaa aaatcaaagg gttgaacgag
     3361 gtgcttaatc tggcaatcca gaaaaatgat gaaaccgccc acatcattgc ttcgttacct
     3421 catcgtttta tcccgttgtt caagcaaatt ttaagtgatc gcaatacgct gtcgtttatt
     3481 ctggaagaat tcaaaagtga tgaagaggta attcagtcgt tttgcaaata taaaaccctg
     3541 ttacgtaacg aaaatgtcct ggaaacagcc gaggctttgt ttaacgaact gaatagcatt
     3601 gacctgacgc atatctttat tagccacaaa aaattagaga ccatctcatc agctctgtgc
     3661 gatcattggg atacactgcg caatgcgctg tatgaacgtc gtatttcgga attgactggc
     3721 aaaatcacta aaagcgcgaa agagaaagta cagcgctcgc ttaaacatga agatatcaac
     3781 ctgcaggaga tcatcagcgc cgcgggtaaa gaactgtcgg aggcatttaa acagaagacg
     3841 agcgagattc tgtcccacgc acatgccgcc ttagaccagc cgctcccgac cactctgaag
     3901 aaacaggaag agaaagaaat ccttaaaagt caactggaca gtttactggg tctctatcat
     3961 ctgctggatt ggtttgcggt agacgaaagc aatgaagtgg atccggagtt tagtgcccgt
     4021 ctgacaggaa tcaagctgga aatggagcct tcgcttagct tctacaacaa agcccgcaat
     4081 tatgccacga aaaaacccta tagtgtcgaa aaatttaaac tcaactttca aatgccgacc
     4141 cttgcgtcgg gctgggatgt caacaaagaa aaaaacaacg gagctattct gttcgttaaa
     4201 aatggtctgt actacctggg catcatgccg aaacagaaag gtcgctacaa agccctttcg
     4261 ttcgagccca cggaaaaaac aagcgaaggc ttcgacaaaa tgtactacga ttactttccg
     4321 gatgcagcaa aaatgatccc gaaatgttcc acacagctga aagccgttac agcacatttt
     4381 cagacgcaca ccacccccat cttactgtcc aacaatttta ttgaaccgct ggagattact
     4441 aaagaaattt atgatttgaa caatccggaa aaagagccaa aaaagtttca aaccgcctac
     4501 gctaaaaaaa ccggggatca gaaagggtac cgcgaagcgt tgtgcaagtg gattgatttc
     4561 acccgcgatt ttctcagtaa atataccaag actacctcga ttgacctgag ctcactgcgc
     4621 ccgagctctc aatataagga tttgggtgag tactatgctg aattaaaccc tttattgtac
     4681 cacatttctt ttcagcgcat cgccgaaaag gaaattatgg acgcagtcga aaccgggaaa
     4741 ctgtacctgt tccagatcta taataaggac ttcgccaaag gacatcatgg caaaccgaac
     4801 ctgcacaccc tttactggac cgggcttttc tctccggaaa atttggcgaa aacctcgatc
     4861 aagcttaacg gtcaagctga gctgttttac cgtccaaaat cccgcatgaa gcgcatggcg
     4921 catcgtttag gtgaaaaaat gctgaataag aaactgaaag atcagaaaac ccctatcccg
     4981 gataccctct accaggaact gtatgattac gtgaaccatc gtctctcgca tgacctgtca
     5041 gacgaagcgc gtgcgttact gcccaatgta atcacaaaag aagtttcgca tgaaattatt
     5101 aaagatcgtc gttttacatc tgataaattc ttttttcatg ttccgatcac cctcaactat
     5161 caggccgcaa acagtccaag taagtttaac cagcgcgtta atgcttacct gaaggaacat
     5221 ccggagactc cgattattgg aattgatcgc ggtgaacgta atttgatcta tatcactgtg
     5281 atcgatagta ccggtaagat tctggagcag cgcagcttga acacaattca acagtttgat
     5341 tatcagaaaa aattagacaa ccgcgaaaaa gagcgcgtgg ctgcccgtca ggcgtggtct
     5401 gttgtcggta ccattaaaga tctgaagcag ggctatcttt ctcaggttat tcacgaaatt
     5461 gtagatctga tgatccatta tcaggcggtt gttgtgttgg agaatctcaa tttcggtttt
     5521 aagagtaagc gcacaggcat cgctgaaaaa gcagtttatc agcagtttga aaaaatgctg
     5581 atcgacaaat tgaactgttt agttctcaaa gattacccag cggaaaaggt gggcggagtg
     5641 ctgaatccgt accaattaac ggatcaattc acttccttcg caaagatggg tacccaaagc
     5701 ggctttctgt tctatgtgcc ggccccgtat acctcgaaaa tcgatccact gacgggcttc
     5761 gtagatccgt tcgtgtggaa aaccattaaa aatcatgaaa gtcgtaaaca ttttctcgaa
     5821 ggcttcgact tcctgcacta cgacgtgaaa actggcgatt tcattctgca ttttaaaatg
     5881 aaccgcaacc tttcgtttca gcgcggtctg ccgggcttta tgccggcttg ggacattgtt
     5941 tttgagaaaa atgaaaccca gtttgatgct aaaggcactc ctttcatcgc cggtaaacgc
     6001 atcgtacctg tgattgaaaa ccatcgtttt acagggcgtt accgtgattt atacccggcg
     6061 aacgaattga tcgcgctgct ggaggaaaag ggcatcgttt tccgtgacgg ctccaatatt
     6121 ctgccgaaat tactggaaaa cgacgattca cacgcaattg ataccatggt cgcactgatt
     6181 cgctcagtct tacagatgcg taactctaat gcagccacag gagaagatta tattaattcg
     6241 ccagtccgcg atttgaacgg tgtttgcttc gacagccgtt ttcagaatcc tgaatggccg
     6301 atggacgctg atgccaacgg agcttatcat atcgccctga aaggccagct cctgctgaac
     6361 cacctgaagg aaagcaaaga tctgaaattg cagaacggca ttagcaacca ggactggtta
     6421 gcatacatcc aggaactgcg taactaataa cattggaagt ggataacgga tccgcgatcg
     6481 cggcgcgcca cctggtggcc ggccggtacc acgcgtgcgc gctgatccgg ctgctaacaa
     6541 agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct
     6601 tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata
     6661 tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag
     6721 cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata
     6781 gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg
     6841 aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag
     6901 catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg
     6961 cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa
     7021 gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag
     7081 gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg
     7141 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     7201 caataaccct gataaatgct tcaataacat tgaaaaagga agagtatgag tattcaacat
     7261 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     7321 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     7381 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     7441 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg
     7501 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     7561 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     7621 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     7681 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     7741 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca
     7801 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     7861 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     7921 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     7981 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     8041 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     8101 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     8161 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     8221 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     8281 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     8341 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     8401 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
     8461 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     8521 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     8581 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     8641 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     8701 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     8761 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     8821 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     8881 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     8941 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     9001 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg
     9061 tattttctcc ttacgcatct gtgcggtatt tcacaccgca atggtgcact ctcagtacaa
     9121 tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt
     9181 catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct
     9241 cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt
     9301 ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg
     9361 aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag
     9421 cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt
     9481 cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg
     9541 agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg
     9601 tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca
     9661 atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc
     9721 gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga
     9781 aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca
     9841 gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa
//
