LOCUS       sequence-217297-        5752 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses N-terminus to aa269 of VCre recombinase fused to FRB CID.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5752)
  AUTHORS   Weinberg BH, Cho JH, Agarwal Y, Pham NTH, Caraballo LD, Walkosz M, 
            Ortega C, Trexler M, Tague N, Law B, Benman WKJ, Letendre J, Beal J,
            Wong WW
  TITLE     High-performance chemical- and light-inducible recombinases in 
            mammalian cells and mice.
  JOURNAL   Nat Commun. 2019 Oct 24;10(1):4845. doi: 10.1038/s41467-019-12800-7.
  PUBMED    31649244
REFERENCE   2  (bases 1 to 5752)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-10-24"; volume: "10(1):4845. doi"; pages: " 
            10.1038/s41467-019-12800-7"
COMMENT     Sequence Label: pBW2584_pCAG-PV1-VCre-N269-L1-FRB-NLS-BGHpA
FEATURES             Location/Qualifiers
     source          1..5752
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     466..488
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     550..569
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             1526..1801
                     /codon_start=1
                     /product="T2098L mutant of FKBP-rapamycin binding domain of
                     human FRAP"
                     /label=FRB*
                     /note="binds synthetic ligands such as AP21967"
                     /translation="ILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTL
                     KETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLLQAWDLYYHVFRRIS"
     CDS             1802..1822
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    1832..2056
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(2296..2315)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    2361..2416
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2415..2434)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(2777..2793)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2777..2793)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2790..2812)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2801..2817
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2825..2831,2832..2849,2850..2855))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2870..2891
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      2976..3110
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    3130..3264
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3167..3186)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3221..3240
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3332..3349)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3503..4091)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3583..3602)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4262..5053,5054..5122))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4885..4904
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5123..5227)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        5258..5637
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tgcacccccc tccccgagtt gctgagcacg gcccggcttc gggtgcgggg ctccgtacgg
       61 ggcgtggcgc ggggctcgcc gtgccgggcg gggggtggcg gcaggtgggg gtgccgggcg
      121 gggcggggcc gcctcgggcc ggggagggct cgggggaggg gcgcggcggc ccccggagcg
      181 ccggcggctg tcgaggcgcg gcgagccgca gccattgcct tttatggtaa tcgtgcgaga
      241 gggcgcaggg acttcctttg tcccaaatct gtgcggagcc gaaatctggg aggcgccgcc
      301 gcaccccctc tagcgggcgc ggggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg
      361 gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct ccctctccag cctcggggct
      421 gtccgcgggg ggacggctgc cttcgggggg gacggggcag ggcggggttc ggcttctggc
      481 gtgtgaccgg cggctctaga gcctctgcta accatgttca tgccttcttc tttttcctac
      541 agctcctggg caacgtgctg gttattgtgc tgtctcatca ttttggcaaa gaattgatta
      601 attcgagcga aggcgcgccc attactcgca tccattctca ggctgtctcg tctcgtctcg
      661 cacgcgtgcc gccaccatga tcgagaacca gctgagcctg ctgggcgact tttctggcgt
      721 gcggcccgac gatgtgaaaa ccgccattca ggccgcccag aaaaagggca tcaacgtggc
      781 cgagaacgag cagttcaagg ccgccttcga gcatctgctg aacgagttca agaagcggga
      841 agagagatac agccccaaca ccctgcggcg gctggaaagc gcctggacct gcttcgtgga
      901 ttggtgcctg gccaaccaca gacacagcct gcctgccacc cccgataccg tggaagcctt
      961 cttcatcgag cgggccgagg aactgcaccg gaacaccctg agcgtgtaca gatgggccat
     1021 cagccgggtg cacagagtgg ccggatgccc tgatccctgc ctggacatct acgtggaaga
     1081 tcggctgaag gccattgccc ggaagaaagt gcgggaaggc gaggccgtga agcaggccag
     1141 ccctttcaac gagcagcatc tgctgaagct gaccagcctg tggtacagaa gcgacaagct
     1201 gctgctgcgg cggaacctgg ctctgctggc tgtggcctac gagagcatgc tgagagccag
     1261 cgagctggcc aacatccggg tgtccgatat ggaactggcc ggcgacggaa ccgccatcct
     1321 gaccatccct atcaccaaga ccaaccactc cggcgagccc gatacctgca tcctgtccca
     1381 ggatgtggtg tccctgctga tggactacac cgaggccggc aagctggata tgagcagcga
     1441 cggcttcctg ttcgtgggcg tgtccaagca caacacctgt atctccggag ggtctggctc
     1501 cggatcaagt ggtggcagcg gtaccatcct ctggcatgag atgtggcatg aaggcctgga
     1561 agaggcatct cgtttgtact ttggggaaag gaacgtgaaa ggcatgtttg aggtgctgga
     1621 gcccttgcat gctatgatgg aacggggccc ccagactctg aaggaaacat cctttaatca
     1681 ggcctatggt cgagatttaa tggaggccca agagtggtgc aggaagtaca tgaaatcagg
     1741 gaatgtcaag gacctcctcc aagcctggga cctctattat catgtgttcc gacgaatctc
     1801 acccaagaaa aagcggaagg tgtgagaatt cctgtgcctt ctagttgcca gccatctgtt
     1861 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
     1921 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt
     1981 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat
     2041 gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc
     2101 cacgcgccct gtagcgtgta ccgctgggag ttcgtagacg gaaacaaacg cagaatccaa
     2161 gcgcggccgc ccaggataca tagattacca caactccgag cccttccacc gctagcgcac
     2221 tcctcaggtg caggctgcct atcagaaggt ggtggctggt gtggccaatg ccctggctca
     2281 caaataccac tgagatcttt ttccctctgc caaaaattat ggggacatca tgaagcccct
     2341 tgagcatctg acttctggct aataaaggaa atttattttc attgcaatag tgtgttggaa
     2401 ttttttgtgt ctctcactcg gaaggacata tgggagggca aatcatttaa aacatcagaa
     2461 tgagtatttg gtttagagtt tggcaacata tgcccatatg ctggctgcca tgaacaaagg
     2521 ttggctataa agaggtcatc agtatatgaa acagccccct gctgtccatt ccttattcca
     2581 tagaaaagcc ttgacttgag gttagatttt ttttatattt tgttttgtgt tatttttttc
     2641 tttaacatcc ctaaaatttt ccttacatgt tttactagcc agatttttcc tcctctcctg
     2701 actactccca gtcatagctg tccctcttct cttatggaga tccctcgacc tgcagcccaa
     2761 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     2821 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     2881 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     2941 agcggatccg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccagttc
     3001 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     3061 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     3121 caaaaagcta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     3181 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     3241 aatgtatctt atcatgtctg gatccgctgc attaatgaat cggccaacgc gcggggagag
     3301 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     3361 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     3421 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     3481 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     3541 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     3601 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     3661 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     3721 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     3781 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     3841 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     3901 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     3961 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     4021 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     4081 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     4141 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     4201 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     4261 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     4321 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     4381 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     4441 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     4501 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     4561 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     4621 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     4681 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     4741 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     4801 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     4861 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     4921 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     4981 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     5041 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     5101 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     5161 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     5221 ttccgcgcac atttccccga aaagtgccac ctgggtcgac attgattatt gactagttat
     5281 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
     5341 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
     5401 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
     5461 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
     5521 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
     5581 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtc
     5641 gaggtgagcc ccacgttctg cttcactctc cccatctccc ccccctcccc acccccaatt
     5701 ttgtatttat ttatttttta attattttgt gcagcgatgg gggcgggggg gg
//
