LOCUS       sequence-217306-        5307 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses aa278 to C-terminus of VCre recombinase fused to FKBP CID.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5307)
  AUTHORS   Weinberg BH, Cho JH, Agarwal Y, Pham NTH, Caraballo LD, Walkosz M, 
            Ortega C, Trexler M, Tague N, Law B, Benman WKJ, Letendre J, Beal J,
            Wong WW
  TITLE     High-performance chemical- and light-inducible recombinases in 
            mammalian cells and mice.
  JOURNAL   Nat Commun. 2019 Oct 24;10(1):4845. doi: 10.1038/s41467-019-12800-7.
  PUBMED    31649244
REFERENCE   2  (bases 1 to 5307)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-10-24"; volume: "10(1):4845. doi"; pages: " 
            10.1038/s41467-019-12800-7"
COMMENT     Sequence Label: pBW2620_pCAG-PV1-FKBP-L1-VCre-278C-NLS-BGHpA
FEATURES             Location/Qualifiers
     source          1..5307
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     466..488
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     550..569
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             686..1006
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     regulatory      1248..1257
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1358..1378
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    1388..1612
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(1852..1871)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    1917..1972
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(1971..1990)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(2333..2349)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2333..2349)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2346..2368)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2357..2373
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2381..2387,2388..2405,2406..2411))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2426..2447
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      2532..2666
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    2686..2820
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2723..2742)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2777..2796
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2888..2905)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3059..3647)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3139..3158)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3818..4609,4610..4678))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4441..4460
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4679..4783)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        4814..5193
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tgcacccccc tccccgagtt gctgagcacg gcccggcttc gggtgcgggg ctccgtacgg
       61 ggcgtggcgc ggggctcgcc gtgccgggcg gggggtggcg gcaggtgggg gtgccgggcg
      121 gggcggggcc gcctcgggcc ggggagggct cgggggaggg gcgcggcggc ccccggagcg
      181 ccggcggctg tcgaggcgcg gcgagccgca gccattgcct tttatggtaa tcgtgcgaga
      241 gggcgcaggg acttcctttg tcccaaatct gtgcggagcc gaaatctggg aggcgccgcc
      301 gcaccccctc tagcgggcgc ggggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg
      361 gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct ccctctccag cctcggggct
      421 gtccgcgggg ggacggctgc cttcgggggg gacggggcag ggcggggttc ggcttctggc
      481 gtgtgaccgg cggctctaga gcctctgcta accatgttca tgccttcttc tttttcctac
      541 agctcctggg caacgtgctg gttattgtgc tgtctcatca ttttggcaaa gaattgatta
      601 attcgagcga aggcgcgccc attactcgca tccattctca ggctgtctcg tctcgtctcg
      661 cacgcgtgcc gccaccatgt ctagaggagt gcaggtggaa accatctccc caggagacgg
      721 gcgcaccttc cccaagcgcg gccagacctg cgtggtgcac tacaccggga tgcttgaaga
      781 tggaaagaaa tttgattcct cccgggacag aaacaagccc tttaagttta tgctaggcaa
      841 gcaggaggtg atccgaggct gggaagaagg ggttgcccag atgagtgtgg gtcagagagc
      901 caaactgact atatctccag attatgccta tggtgccact gggcacccag gcatcatccc
      961 accacatgcc actctcgtct tcgatgtgga gcttctaaaa ctggaatccg gagggtctgg
     1021 ctccggatca agtggtggca gcggtaccgg cgaggtgctg cacaagccca tcaccaccaa
     1081 gacagtggaa ggcgtgttct acagcgcctg ggagacactg gacctgggca gacagggcgt
     1141 gaagcctttc acagcccaca gcgccagagt gggagccgct caggacctgc tgaagaaggg
     1201 ctacaatacc ctgcagatcc agcagtccgg ccggtggtct agcggagcca tggtggccag
     1261 atacggcaga gccatcctgg ctagggatgg cgctatggcc cacagcagag tgaaaaccag
     1321 atccgccccc atgcagtggg gcaaggacga gaaggacccc aagaaaaagc ggaaggtgtg
     1381 agaattcctg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
     1441 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
     1501 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
     1561 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag
     1621 gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag cgtgtaccgc
     1681 tgggagttcg tagacggaaa caaacgcaga atccaagcgc ggccgcccag gatacataga
     1741 ttaccacaac tccgagccct tccaccgcta gcgcactcct caggtgcagg ctgcctatca
     1801 gaaggtggtg gctggtgtgg ccaatgccct ggctcacaaa taccactgag atctttttcc
     1861 ctctgccaaa aattatgggg acatcatgaa gccccttgag catctgactt ctggctaata
     1921 aaggaaattt attttcattg caatagtgtg ttggaatttt ttgtgtctct cactcggaag
     1981 gacatatggg agggcaaatc atttaaaaca tcagaatgag tatttggttt agagtttggc
     2041 aacatatgcc catatgctgg ctgccatgaa caaaggttgg ctataaagag gtcatcagta
     2101 tatgaaacag ccccctgctg tccattcctt attccataga aaagccttga cttgaggtta
     2161 gatttttttt atattttgtt ttgtgttatt tttttcttta acatccctaa aattttcctt
     2221 acatgtttta ctagccagat ttttcctcct ctcctgacta ctcccagtca tagctgtccc
     2281 tcttctctta tggagatccc tcgacctgca gcccaagctt ggcgtaatca tggtcatagc
     2341 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     2401 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     2461 cactgcccgc tttccagtcg ggaaacctgt cgtgccagcg gatccgcatc tcaattagtc
     2521 agcaaccata gtcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     2581 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     2641 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctaactt gtttattgca
     2701 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     2761 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc
     2821 cgctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     2881 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     2941 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     3001 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     3061 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     3121 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     3181 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     3241 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     3301 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     3361 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     3421 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     3481 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     3541 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     3601 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     3661 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     3721 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     3781 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     3841 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     3901 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     3961 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     4021 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     4081 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     4141 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     4201 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     4261 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     4321 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     4381 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     4441 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     4501 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     4561 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     4621 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     4681 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     4741 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     4801 tgccacctgg gtcgacattg attattgact agttattaat agtaatcaat tacggggtca
     4861 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
     4921 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
     4981 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
     5041 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
     5101 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
     5161 tacatctacg tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc
     5221 actctcccca tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta
     5281 ttttgtgcag cgatgggggc ggggggg
//
