LOCUS       sequence-217312-       11340 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses wild-type Rpb1 in yeast cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11340)
  AUTHORS   Morrill SA, Exner AE, Babokhov M, Reinfeld BI, Fuchs SM
  TITLE     DNA Instability Maintains the Repeat Length of the Yeast RNA 
            Polymerase II C-terminal Domain.
  JOURNAL   J Biol Chem. 2016 May 27;291(22):11540-50. doi: 
            10.1074/jbc.M115.696252. Epub 2016 Mar 29.
  PUBMED    27026700
REFERENCE   2  (bases 1 to 11340)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M115.696252"; journalName: "J Biol Chem"; date: 
            "2016-05-27- 27"; volume: "291"; issue: "22"; pages: "11540-50"
COMMENT     Sequence Label: pSMF2
FEATURES             Location/Qualifiers
     source          1..11340
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..28)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    70..573
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        610..714
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(715..783,784..1575)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(933..952)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1746..2334
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2235..2254
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2488..2505
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2622..2643
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2658..2663,2664..2681,2682..2688)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2696..2712
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2701..2723
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2720..2736
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2720..2736
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        9177..9582
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             9583..10677
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     primer_bind     complement(11171..11190)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     11290..11312
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttaggacgg atcgcttgcc tgtaacttac acgcgcctcg tatcttttaa tgatggaata
      121 atttgggaat ttactctgtg tttatttatt tttatgtttt gtatttggat tttagaaagt
      181 aaataaagaa ggtagaagag ttacggaatg aagaaaaaaa aataaacaaa ggtttaaaaa
      241 atttcaacaa aaagcgtact ttacatatat atttattaga caagaaaagc agattaaata
      301 gatatacatt cgattaacga taagtaaaat gtaaaatcac aggattttcg tgtgtggtct
      361 tctacacaga caagatgaaa caattcggca ttaatacctg agagcaggaa gagcaagata
      421 aaaggtagta tttgttggcg atccccctag agtcttttac atcttcggaa aacaaaaact
      481 attttttctt taatttcttt ttttactttc tatttttaat ttatatattt atattaaaaa
      541 atttaaatta taattatttt tatagcacgt gatgaaaagg acccaggtgg cacttttcgg
      601 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
      661 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
      721 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
      781 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
      841 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
      901 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
      961 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     1021 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     1081 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     1141 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     1201 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     1261 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     1321 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     1381 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     1441 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     1501 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     1561 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     1621 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     1681 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     1741 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     1801 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     1861 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     1921 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     1981 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     2041 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     2101 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     2161 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     2221 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     2281 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     2341 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     2401 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     2461 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     2521 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     2581 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
     2641 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
     2701 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcgggt
     2761 tgcgcgtctc gaggagcgtg cagcaccatt caccgccagc tttgtatcgt cctttagagt
     2821 gcagcaagaa cattaattgc tctcacagag gtatcataag aacatccgat tcgttcatta
     2881 actgaaatca tatctgtgtt gttctgatcg actctctttg ttagttttat cttccatttt
     2941 tttttattcc tttcattttc caataaaatt tgaacaggat cgatataaac agataaaaac
     3001 cctccaaagt taaccatttg tttatatttg cttttctgca taagttttcc tagcgaattc
     3061 cctgatcaac tttcaaggaa aactaaaact actgtattat aagaggtttt ttttttcatt
     3121 ccagattaat tttgaaatac gatatcctca agtttatcta ccagaatatt tgactaagaa
     3181 atcaaactct gttaataata atataattat aaaaacctca actagaaact ccaaaaaaaa
     3241 aaatttacca ttttttactt tctatccttg ttaaccaaat ttcaaaaaaa ttttaccttt
     3301 tctttttcca gaagagggac caatcataaa gatagcaata acactttacc ccaaaatata
     3361 aatcagacat ggtaggacaa cagtattcta gtgctccact ccgtacagta aaagaggtcc
     3421 aattcggtct tttctcacct gaagaagtta gagcaatcag tgtggccaaa attagatttc
     3481 cagagacaat ggatgaaacc cagacgagag cgaaaattgg tggtctaaac gaccctaggt
     3541 taggctctat tgatcgtaat ctgaagtgtc aaacttgtca agagggtatg aacgaatgtc
     3601 ctggtcattt tggtcacata gatttagcaa aacctgtatt tcatgttggt tttattgcca
     3661 aaattaagaa agtatgtgag tgtgtctgta tgcactgtgg taagctatta ctggatgaac
     3721 ataatgaatt aatgagacaa gctctagcaa tcaaagacag taaaaaaagg tttgctgcaa
     3781 tttggacttt atgtaaaaca aaaatggtct gcgaaacaga tgtcccttct gaagatgatc
     3841 ctactcagct cgtatcaagg ggaggttgtg gtaatacaca gcctacaatt cgtaaggatg
     3901 ggttgaaatt agttggtagt tggaaaaaag atagagccac gggggatgcg gatgaaccag
     3961 aactaagagt tttaagtacg gaggaaatct tgaatatttt taagcatatc tcagtaaaag
     4021 acttcactag tttgggtttc aacgaagttt tttctcgtcc agaatggatg attttaacat
     4081 gccttcctgt cccaccacca ccggtgcgtc catccatttc cttcaatgaa tctcaaagag
     4141 gtgaggatga tttaaccttt aaacttgctg atattttaaa agctaatatt agtttggaaa
     4201 cactagagca taacggtgct ccacatcatg ctattgaaga agcagagagt ttattacaat
     4261 ttcatgttgc cacttatatg gataatgata ttgctggtca accacaagct cttcaaaagt
     4321 ccggccgtcc cgttaaatct attcgtgctc gtttgaaggg taaagagggt cgtatcagag
     4381 gtaatttaat gggtaagcgt gtggattttt cggcaagaac tgttatttct ggtgatccta
     4441 atttggaatt agaccaagtc ggtgttccaa aatctattgc caagacttta acatacccag
     4501 aagtggtcac accatataac atagatcgtc tgacgcaact tgttaggaat ggaccaaatg
     4561 agcaccccgg tgccaaatac gtcattcgtg atagcggaga ccgtatagat ttaagataca
     4621 gtaaaagggc aggtgatatt caattacagt atgggtggaa agttgaacgt catattatgg
     4681 acaatgatcc agttttattc aaccgtcaac cttcgttgca caaaatgtcc atgatggccc
     4741 acagagtaaa agttattcca tattctacat ttagattgaa tttgtccgtt acatctccat
     4801 acaatgccga tttcgacggt gacgaaatga atcttcacgt tcctcagtct gaggaaacaa
     4861 gggcggaact ttctcaatta tgtgctgttc ctctgcaaat tgtttcacca caatctaaca
     4921 aaccttgtat gggtattgtt caagatactt tgtgtggtat tcgtaaactg acattaagag
     4981 atacatttat agaacttgat caagttttga atatgcttta ttgggttcca gattgggatg
     5041 gtgttattcc gacacctgca attatcaagc ccaaaccttt gtggtccggt aaacaaatct
     5101 tgtctgtggc tatcccaaac ggtattcatt tacaacgttt tgatgagggc actactctgc
     5161 tttctccaaa ggataatggt atgcttatta ttgacggtca aatcattttt ggtgtagtag
     5221 agaaaaaaac cgttggttcc tccaatggtg gtttaattca tgttgttacg agagaaaagg
     5281 gacctcaagt ttgtgctaag ttgtttggta acatacagaa agttgttaac ttttggttac
     5341 tacataatgg gttttcaaca ggtattggtg ataccattgc ggacggccca acaatgaggg
     5401 aaattacaga gacaattgca gaggctaaaa agaaagtttt ggatgttacg aaagaagccc
     5461 aggcaaactt attgactgct aaacatggta tgactctccg tgagtctttt gaggataacg
     5521 ttgttcggtt cctaaatgaa gcaagagata aggcaggtcg tttagctgaa gtcaatttga
     5581 aagatttgaa caatgtgaaa caaatggtta tggcaggttc caagggttca tttattaata
     5641 tcgcgcaaat gtcagcttgt gtaggacagc aatctgttga aggtaaacgt attgcttttg
     5701 ggttcgttga tcgtacctta cctcatttct ctaaagatga ttactcccca gagtctaaag
     5761 gttttgttga gaactcatat ttgagaggtt tgaccccaca agaatttttt ttccatgcaa
     5821 tgggtggtcg tgaaggtctt atcgataccg ccgtcaaaac agccgaaaca ggttatattc
     5881 aacgtcgttt agtgaaagct ctagaagata tcatggttca ttacgataac accacaagaa
     5941 actcattggg taacgttatt cagtttattt atggtgaaga tggtatggat gctgcgcata
     6001 ttgaaaagca atcgctagat actattggtg gctccgatgc agcttttgaa aagagataca
     6061 gagttgattt attgaataca gaccataccc ttgatccctc actattggaa tccggatctg
     6121 agatacttgg cgatttgaaa cttcaagttc tcctggatga agaatacaaa caattagtga
     6181 aagatcgtaa atttttgagg gaagtttttg ttgatggtga agcaaactgg ccattaccag
     6241 tcaacataag acgtattatt caaaatgctc aacaaacttt ccacatagat catacgaaac
     6301 catctgattt aacaatcaaa gacatcgttc ttggtgtaaa ggatttgcaa gaaaacttat
     6361 tagtgttgcg tggtaagaat gaaattatac aaaatgccca gcgagatgca gttacattgt
     6421 tctgctgttt attacgttcc cgtttggcca cacgtagagt tctacaagag tacagactaa
     6481 caaaacaggc attcgattgg gtattaagta atatcgaggc acaattcctc cgttctgttg
     6541 ttcaccctgg tgaaatggtt ggtgttctag cagcccaatc cattggtgaa ccagccacac
     6601 aaatgaccct taacaccttc cattttgctg gtgttgcttc caaaaaagtt acttctggtg
     6661 tcccccgttt aaaggaaatt ttgaatgtgg ccaaaaacat gaaaacccct tccttgactg
     6721 tatacttaga gcctggtcat gctgccgatc aagaacaagc gaagttgatc agatctgcta
     6781 tcgagcatac cactttaaag agtgtcacta ttgcttcaga aatttactat gatcctgatc
     6841 cacgttccac agttattcca gaagatgaag aaattatcca acttcatttc tcattattgg
     6901 atgaagaggc tgaacaatct tttgaccaac aatcaccttg gttattacgt ctggaactgg
     6961 atcgtgcagc aatgaatgat aaagacttaa caatgggtca ggttggtgaa agaatcaagc
     7021 aaacattcaa aaatgatttg tttgttatct ggtctgaaga caacgatgag aagttgatca
     7081 tccgttgtcg tgttgttcgt ccaaagtcac tagatgctga gactgaagca gaagaagatc
     7141 atatgttgaa gaaaattgag aacacaatgt tagagaatat tacattacgt ggtgtagaga
     7201 acatcgagcg tgttgtcatg atgaaatatg accgtaaagt accaagtcca actggtgaat
     7261 acgttaagga acctgaatgg gtgttggaaa cagatggtgt taacttatct gaagttatga
     7321 ctgttcctgg tatcgaccca accagaatct ataccaactc cttcattgat ataatggaag
     7381 ttctaggtat tgaagctggt cgtgcagcct tgtataaaga agtttacaat gttattgctt
     7441 ctgatggttc gtatgttaac taccgtcata tggctttgtt agtcgatgtt atgacaaccc
     7501 aaggtggctt aacttctgtt actcgtcatg gtttcaacag atcaaataca ggtgccttaa
     7561 tgagatgttc atttgaagaa actgtcgaaa ttttgtttga agctggtgct tcagccgaat
     7621 tagatgattg tcgtggtgtt tcggaaaatg tcattcttgg tcaaatggct ccaatcggta
     7681 ccggtgcatt tgatgtgatg atcgatgagg agtcactggt aaaatacatg ccagaacaaa
     7741 aaataactga gattgaagac ggacaagatg gtggcgtcac accatacagt aacgaaagtg
     7801 gtttggtcaa tgcagatctt gacgttaaag atgagctaat gttttcacct ctggttgatt
     7861 cgggttcaaa tgacgctatg gctggaggat ttacagcgta cggtggtgct gattatggtg
     7921 aagccacgtc tccatttggt gcttatggtg aagcacctac atctcccggg tttggagtct
     7981 cctcaccagg cttttctcca acttccccaa catactctcc tacctctcca gcgtactcac
     8041 caacatcacc atcgtactca ccaacatcac catcgtactc gccaacatca ccatcgtact
     8101 cacctacatc accatcgtat tcaccaacgt caccatcata ttcgccaacg tcaccatcat
     8161 attcgccaac gtcgccatcg tattctccaa cgtcaccatc gtattcgcca acgtcgcctt
     8221 cctactctcc cacgtcgcca agctacagcc ctacgtctcc ttcttattct cctacatctc
     8281 catcatactc tcctacgtca ccaagttaca gcccaacgtc accaagttac agcccaacgt
     8341 ctccagccta ttccccaaca tcaccaagtt atagtcctac atcgccttca tactctccaa
     8401 catcaccatc ctattcccca acatcacctt cttactctcc cacctctcca aactatagcc
     8461 ctacttcacc ttcttactcc ccaacatctc caggctacag cccaggatct cctgcatatt
     8521 ctccaaagca agacgaacaa aagcataatg aaaatgaaaa ttccagatga gctcgtatat
     8581 catccttacg tatttgacgt tattacatta tatatagttt ctcaaataat atttctagtt
     8641 tatttttgta tcataataaa aacgtatacc aaatatacca ttatttttca tagcattatg
     8701 gtagggatag ggaatcaagt aactaattta tatccgcaga gcattgggaa aaccaacggc
     8761 gctagtaaat gcatttaaat tacgtccgtc caacttctaa gcttcaagtg gtagactctt
     8821 aactctgaac ctttttagca attaagctct tgaagatatc aaaagtgtta ccgtccggct
     8881 gtaaattata aacgtttcct gtaaattgag tggaactacc gcttaccaat tcttttgcaa
     8941 tcagtaaacc gtagtcttcc gtgataccag taatcatggc ttgcgtattt ccgtgatctg
     9001 gtaatgttac tatttggtta ctatgtaacc acaactcata ataacttggc aatatttccg
     9061 cagctccgta gttaataaac tgttttaata tgacctcaag gttattcata tagagtgcct
     9121 gcgggcccga tcccgatatc caggcctccc gatatccagg cctcagctta gcctagaact
     9181 gtgggaatac tcaggtatcg taagatgcaa gagttcgaat ctcttagcaa ccattatttt
     9241 tttcctcaac ataacgagaa cacacagggg cgctatcgca cagaatcaaa ttcgatgact
     9301 ggaaattttt tgttaatttc agaggtcgcc tgacgcatat acctttttca actgaaaaat
     9361 tgggagaaaa aggaaaggtg agagcgccgg aaccggcttt tcatatagaa tagagaagcg
     9421 ttcatgacta aatgcttgca tcacaatact tgaagttgac aatattattt aaggacctat
     9481 tgttttttcc aataggtggt tagcaatcgt cttactttct aacttttctt accttttaca
     9541 tttcagcaat atatatatat atttcaagga tataccattc taatgtctgc ccctaagaag
     9601 atcgtcgttt tgccaggtga ccacgttggt caagaaatca cagccgaagc cattaaggtt
     9661 cttaaagcta tttctgatgt tcgttccaat gtcaagttcg atttcgaaaa tcatttaatt
     9721 ggtggtgctg ctatcgatgc tacaggtgtt ccacttccag atgaggcgct ggaagcctcc
     9781 aagaaggctg atgccgtttt gttaggtgct gtgggtggtc ctaaatgggg tacaggtagt
     9841 gttagacctg aacaaggttt actaaaaatc cgtaaagaac ttcaattgta cgccaactta
     9901 agaccatgta actttgcatc cgactctctt ttagacttat ctccaatcaa gccacaattt
     9961 gctaaaggta ctgacttcgt tgttgtcaga gaattagtgg gaggtattta ctttggtaag
    10021 agaaaggaag acgatggtga tggtgtcgct tgggatagtg aacaatacac cgttccagaa
    10081 gtgcaaagaa tcacaagaat ggccgctttc atggccctac aacatgagcc accattgcct
    10141 atttggtcct tggataaagc taatgttttg gcctcttcaa gattatggag aaaaactgtg
    10201 gaggaaacca tcaagaacga atttcctaca ttgaaggttc aacatcaatt gattgattct
    10261 gccgccatga tcctagttaa gaacccaacc cacctaaatg gtattataat caccagcaac
    10321 atgtttggtg atatcatctc cgatgaagcc tccgttatcc caggttcctt gggtttgttg
    10381 ccatctgcgt ccttggcctc tttgccagac aagaacaccg catttggttt gtacgaacca
    10441 tgccacggtt ctgctccaga tttgccaaag aataaggtca accctatcgc cactatcttg
    10501 tctgctgcaa tgatgttgaa attgtcattg aacttgcctg aagaaggtaa ggccattgaa
    10561 gatgcagtta aaaaggtttt ggatgcaggt atcagaactg gtgatttagg tggttccaac
    10621 agtaccaccg aagtcggtga tgctgtcgcc gaagaagtta agaaaatcct tgcttaaaaa
    10681 gattctcttt ttttatgata tttgtacata aactttataa atgaaattca taatagaaac
    10741 gacacgaaat tacaaaatgg aatatgttca tagggtagac gaaactatat acgcaatcta
    10801 catacattta tcaagaagga gaaaaaggag gatgtaaagg aatacaggta agcaaattga
    10861 tactaatggc tcaacgtgat aaggaaaaag aattgcactt taacattaat attgacaagg
    10921 aggagggcac cacacaaaaa gttaggtgta acagaaaatc atgaaactat gattcctaat
    10981 ttatatattg gaggattttc tctaaaaaaa aaaaaataca acaaataaaa aacactcaat
    11041 gacctgacca tttgatggag tttaagtcaa taccttcttg aaccatttcc cataatggtg
    11101 aaagttccct caagaatttt actctgtcag aaacggcctt aacgacgtag tcgatatggt
    11161 gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa
    11221 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
    11281 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
//
