LOCUS       sequence-217313-        5568 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses aa270 to C-terminus of VCre recombinase fused to PYL CID.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5568)
  AUTHORS   Weinberg BH, Cho JH, Agarwal Y, Pham NTH, Caraballo LD, Walkosz M, 
            Ortega C, Trexler M, Tague N, Law B, Benman WKJ, Letendre J, Beal J,
            Wong WW
  TITLE     High-performance chemical- and light-inducible recombinases in 
            mammalian cells and mice.
  JOURNAL   Nat Commun. 2019 Oct 24;10(1):4845. doi: 10.1038/s41467-019-12800-7.
  PUBMED    31649244
REFERENCE   2  (bases 1 to 5568)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-10-24"; volume: "10(1):4845. doi"; pages: " 
            10.1038/s41467-019-12800-7"
COMMENT     Sequence Label: pBW2589_pCAG-PV1-PYL-L1-VCre-270C-NLS-BGHpA
FEATURES             Location/Qualifiers
     source          1..5568
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     466..488
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     550..569
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      671..680
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1217..1243
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     regulatory      1509..1518
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1619..1639
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    1649..1873
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(2113..2132)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    2178..2233
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2232..2251)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(2594..2610)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2594..2610)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2607..2629)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2618..2634
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2642..2648,2649..2666,2667..2672))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2687..2708
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      2793..2927
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    2947..3081
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2984..3003)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3038..3057
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3149..3166)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3320..3908)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3400..3419)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4079..4870,4871..4939))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4702..4721
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4940..5044)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        5075..5454
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tgcacccccc tccccgagtt gctgagcacg gcccggcttc gggtgcgggg ctccgtacgg
       61 ggcgtggcgc ggggctcgcc gtgccgggcg gggggtggcg gcaggtgggg gtgccgggcg
      121 gggcggggcc gcctcgggcc ggggagggct cgggggaggg gcgcggcggc ccccggagcg
      181 ccggcggctg tcgaggcgcg gcgagccgca gccattgcct tttatggtaa tcgtgcgaga
      241 gggcgcaggg acttcctttg tcccaaatct gtgcggagcc gaaatctggg aggcgccgcc
      301 gcaccccctc tagcgggcgc ggggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg
      361 gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct ccctctccag cctcggggct
      421 gtccgcgggg ggacggctgc cttcgggggg gacggggcag ggcggggttc ggcttctggc
      481 gtgtgaccgg cggctctaga gcctctgcta accatgttca tgccttcttc tttttcctac
      541 agctcctggg caacgtgctg gttattgtgc tgtctcatca ttttggcaaa gaattgatta
      601 attcgagcga aggcgcgccc attactcgca tccattctca ggctgtctcg tctcgtctcg
      661 cacgcgtgcc gccaccatgg cgccaactca agacgagttc acccaactct cccaatcaat
      721 cgccgagttc cacacgtacc aactcggtaa cggccgttgc tcatctctcc tagctcagcg
      781 aatccacgcg ccgccggaaa cagtatggtc cgtggtgaga cgtttcgata ggccacagat
      841 ttacaaacac ttcatcaaaa gctgtaacgt gagtgaagat ttcgagatgc gagtgggatg
      901 cacgcgcgac gtgaacgtga taagtggatt accggcgaat acgtctcgag agagattaga
      961 tctgttggac gatgatcgga gagtgactgg gtttagtata accggtggtg aacataggct
     1021 gaggaattat aaatcggtta cgacggttca tagatttgag aaagaagaag aagaagaaag
     1081 gatctggacc gttgttttgg aatcttatgt tgttgatgta ccggaaggta attcggagga
     1141 agatacgaga ttgtttgctg atacggttat tagattgaat cttcagaaac ttgcttcgat
     1201 cactgaagct atgaactacc catacgatgt tccagattac gcttccggag ggtctggctc
     1261 cggatcaagt ggtggcagcg gtaccaagcc caagaaggac aagcagaccg gcgaggtgct
     1321 gcacaagccc atcaccacca agacagtgga aggcgtgttc tacagcgcct gggagacact
     1381 ggacctgggc agacagggcg tgaagccttt cacagcccac agcgccagag tgggagccgc
     1441 tcaggacctg ctgaagaagg gctacaatac cctgcagatc cagcagtccg gccggtggtc
     1501 tagcggagcc atggtggcca gatacggcag agccatcctg gctagggatg gcgctatggc
     1561 ccacagcaga gtgaaaacca gatccgcccc catgcagtgg ggcaaggacg agaaggaccc
     1621 caagaaaaag cggaaggtgt gagaattcct gtgccttcta gttgccagcc atctgttgtt
     1681 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
     1741 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
     1801 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
     1861 gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac
     1921 gcgccctgta gcgtgtaccg ctgggagttc gtagacggaa acaaacgcag aatccaagcg
     1981 cggccgccca ggatacatag attaccacaa ctccgagccc ttccaccgct agcgcactcc
     2041 tcaggtgcag gctgcctatc agaaggtggt ggctggtgtg gccaatgccc tggctcacaa
     2101 ataccactga gatctttttc cctctgccaa aaattatggg gacatcatga agccccttga
     2161 gcatctgact tctggctaat aaaggaaatt tattttcatt gcaatagtgt gttggaattt
     2221 tttgtgtctc tcactcggaa ggacatatgg gagggcaaat catttaaaac atcagaatga
     2281 gtatttggtt tagagtttgg caacatatgc ccatatgctg gctgccatga acaaaggttg
     2341 gctataaaga ggtcatcagt atatgaaaca gccccctgct gtccattcct tattccatag
     2401 aaaagccttg acttgaggtt agattttttt tatattttgt tttgtgttat ttttttcttt
     2461 aacatcccta aaatttttct tacatgtttt actagccaga tttttcctcc tctcctgact
     2521 actcccagtc atagctgtcc ctcttctctt atggagatcc ctcgacctgc agcccaagct
     2581 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     2641 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     2701 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     2761 ggatccgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccagttccgc
     2821 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     2881 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
     2941 aaagctaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     3001 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     3061 gtatcttatc atgtctggat ccgctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     3121 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     3181 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     3241 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     3301 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     3361 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     3421 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     3481 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     3541 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     3601 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     3661 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     3721 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     3781 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     3841 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     3901 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     3961 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     4021 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     4081 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     4141 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     4201 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     4261 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     4321 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     4381 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     4441 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     4501 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     4561 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     4621 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     4681 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     4741 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     4801 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     4861 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     4921 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     4981 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     5041 cgcgcacatt tccccgaaaa gtgccacctg ggtcgacatt gattattgac tagttattaa
     5101 tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg cgttacataa
     5161 cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata
     5221 atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag
     5281 tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc
     5341 cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta
     5401 tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac catggtcgag
     5461 gtgagcccca cgttctgctt cactctcccc atctcccccc cctccccacc cccaattttg
     5521 tatttattta ttttttaatt attttgtgca gcgatggggg cggggggg
//
