LOCUS       sequence-21732-e         657 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 657)
  TITLE     Buchler Lab plasmids
REFERENCE   2  (bases 1 to 657)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p406MET25
FEATURES             Location/Qualifiers
     source          1..657
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     304..320
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     304..320
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(354..370)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(355..371)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     terminator      373..620
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(389..407)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(638..657)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(639..657)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gcacgttgaa gctgtcgata ttggggaact gtggtggttg gcaaattgac taattaagtt
       61 agtcaaggcg ccatcctcat gaaaactgtg taacataata accgaagtgt cgaaaaggtg
      121 gcaccttgtc caattcgaac acgctcgatg aaaaaaataa gatatatata aggttaagta
      181 aagcgtctgt tagaaaggaa gtttttcctt tttcttgctc tcttgtcttt tcatctacta
      241 tttccttcgt gtaatacagg gtcgtcagat acatagatac aattctatta cccccatcca
      301 tactctagaa ctagtggatc ccccgggctg caggaattcg atatcaagct tatcgatacc
      361 gtcgacctcg agtcatgtaa ttagttatgt cacgcttaca ttcacgccct ccccccacat
      421 ccgctctaac cgaaaaggaa ggagttagac aacctgaagt ctaggtccct atttattttt
      481 ttatagttat gttagtatta agaacgttat ttatatttca aatttttctt ttttttctgt
      541 acagacgcgt gtacgcatgt aacattatac tgaaaacctt gcttgagaag gttttgggac
      601 gctcgaaggc tttaatttgc ggccggtacc caattcgccc tatagtgagt cgtatta
//
