LOCUS       sequence-217323-        5643 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses N-terminus to aa233 of PhiC31 integrase fused to FRB CID.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5643)
  AUTHORS   Weinberg BH, Cho JH, Agarwal Y, Pham NTH, Caraballo LD, Walkosz M, 
            Ortega C, Trexler M, Tague N, Law B, Benman WKJ, Letendre J, Beal J,
            Wong WW
  TITLE     High-performance chemical- and light-inducible recombinases in 
            mammalian cells and mice.
  JOURNAL   Nat Commun. 2019 Oct 24;10(1):4845. doi: 10.1038/s41467-019-12800-7.
  PUBMED    31649244
REFERENCE   2  (bases 1 to 5643)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-10-24"; volume: "10(1):4845. doi"; pages: " 
            10.1038/s41467-019-12800-7"
COMMENT     Sequence Label: pBW1340_pCAG-PV1-PhiC31-N233-L1-FRB-NLS-BGHpA
FEATURES             Location/Qualifiers
     source          1..5643
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     466..488
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     550..569
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      671..680
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1418..1693
                     /codon_start=1
                     /product="T2098L mutant of FKBP-rapamycin binding domain of
                     human FRAP"
                     /label=FRB*
                     /note="binds synthetic ligands such as AP21967"
                     /translation="ILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTL
                     KETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLLQAWDLYYHVFRRIS"
     CDS             1694..1714
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    1724..1948
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(2188..2207)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    2253..2308
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2307..2326)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(2669..2685)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2669..2685)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2682..2704)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2693..2709
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2717..2723,2724..2741,2742..2747))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2762..2783
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      2868..3002
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    3022..3156
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3059..3078)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3113..3132
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3224..3241)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3395..3983)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3475..3494)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4154..4945,4946..5014))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4777..4796
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5015..5119)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        5150..5529
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tgcacccccc tccccgagtt gctgagcacg gcccggcttc gggtgcgggg ctccgtacgg
       61 ggcgtggcgc ggggctcgcc gtgccgggcg gggggtggcg gcaggtgggg gtgccgggcg
      121 gggcggggcc gcctcgggcc ggggagggct cgggggaggg gcgcggcggc ccccggagcg
      181 ccggcggctg tcgaggcgcg gcgagccgca gccattgcct tttatggtaa tcgtgcgaga
      241 gggcgcaggg acttcctttg tcccaaatct gtgcggagcc gaaatctggg aggcgccgcc
      301 gcaccccctc tagcgggcgc ggggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg
      361 gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct ccctctccag cctcggggct
      421 gtccgcgggg ggacggctgc cttcgggggg gacggggcag ggcggggttc ggcttctggc
      481 gtgtgaccgg cggctctaga gcctctgcta accatgttca tgccttcttc tttttcctac
      541 agctcctggg caacgtgctg gttattgtgc tgtctcatca ttttggcaaa gaattgatta
      601 attcgagcga aggcgcgccc attactcgca tccattctca ggctgtctcg tctcgtctcg
      661 cacgcgtgcc gccaccatgg atacctacgc cggagcctac gacagacaga gccgggagag
      721 agagaacagc agcgccgcca gccccgccac ccagagaagc gccaacgagg ataaggccgc
      781 cgatctgcag agagaggtgg agagggacgg cggcagattc agatttgtgg gccacttcag
      841 cgaggcccct ggcaccagcg ccttcggcac cgccgagaga cccgagttcg agagaatcct
      901 gaacgagtgt agggccggca ggctgaacat gatcatcgtg tacgacgtgt cccggttcag
      961 caggctgaag gtgatggacg ccatccctat cgtgtccgag ctgctggccc tgggcgtgac
     1021 catcgtgtcc acccaggaag gcgtctttag acagggcaac gtgatggacc tgatccacct
     1081 gatcatgagg ctggacgcca gccacaagga gagcagcctg aagagcgcca agatcctgga
     1141 caccaagaac ctgcagaggg agctgggcgg ctatgtgggc ggcaaggccc cctacggctt
     1201 cgagctggtg tccgagacca aggagatcac ccggaacggc aggatggtga acgtggtgat
     1261 caacaagctg gcccacagca ccacccccct gaccggcccc ttcgagtttg agcccgacgt
     1321 gatcaggtgg tggtggcggg agatcaagac ccacaagcac ctgcctttca agccctccgg
     1381 agggtctggc tccggatcaa gtggtggcag cggtaccatc ctctggcatg agatgtggca
     1441 tgaaggcctg gaagaggcat ctcgtttgta ctttggggaa aggaacgtga aaggcatgtt
     1501 tgaggtgctg gagcccttgc atgctatgat ggaacggggc ccccagactc tgaaggaaac
     1561 atcctttaat caggcctatg gtcgagattt aatggaggcc caagagtggt gcaggaagta
     1621 catgaaatca gggaatgtca aggacctcct ccaagcctgg gacctctatt atcatgtgtt
     1681 ccgacgaatc tcacccaaga aaaagcggaa ggtgtgagaa ttcctgtgcc ttctagttgc
     1741 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc
     1801 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct
     1861 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg
     1921 catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct
     1981 agggggtatc cccacgcgcc ctgtagcgtg taccgctggg agttcgtaga cggaaacaaa
     2041 cgcagaatcc aagcgcggcc gcccaggata catagattac cacaactccg agcccttcca
     2101 ccgctagcgc actcctcagg tgcaggctgc ctatcagaag gtggtggctg gtgtggccaa
     2161 tgccctggct cacaaatacc actgagatct ttttccctct gccaaaaatt atggggacat
     2221 catgaagccc cttgagcatc tgacttctgg ctaataaagg aaatttattt tcattgcaat
     2281 agtgtgttgg aattttttgt gtctctcact cggaaggaca tatgggaggg caaatcattt
     2341 aaaacatcag aatgagtatt tggtttagag tttggcaaca tatgcccata tgctggctgc
     2401 catgaacaaa ggttggctat aaagaggtca tcagtatatg aaacagcccc ctgctgtcca
     2461 ttccttattc catagaaaag ccttgacttg aggttagatt ttttttatat tttgttttgt
     2521 gttatttttt tctttaacat ccctaaaatt ttccttacat gttttactag ccagattttt
     2581 cctcctctcc tgactactcc cagtcatagc tgtccctctt ctcttatgga gatccctcga
     2641 cctgcagccc aagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     2701 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     2761 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     2821 acctgtcgtg ccagcggatc cgcatctcaa ttagtcagca accatagtcc cgcccctaac
     2881 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     2941 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     3001 cctaggcttt tgcaaaaagc taacttgttt attgcagctt ataatggtta caaataaagc
     3061 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     3121 tccaaactca tcaatgtatc ttatcatgtc tggatccgct gcattaatga atcggccaac
     3181 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     3241 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     3301 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     3361 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     3421 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     3481 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     3541 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     3601 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     3661 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     3721 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     3781 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     3841 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     3901 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     3961 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     4021 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     4081 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     4141 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     4201 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     4261 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     4321 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     4381 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     4441 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     4501 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     4561 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     4621 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     4681 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     4741 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     4801 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     4861 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     4921 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     4981 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     5041 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     5101 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgggtcg acattgatta
     5161 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
     5221 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
     5281 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
     5341 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
     5401 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
     5461 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
     5521 attaccatgg tcgaggtgag ccccacgttc tgcttcactc tccccatctc ccccccctcc
     5581 ccacccccaa ttttgtattt atttattttt taattatttt gtgcagcgat gggggcgggg
     5641 ggg
//
