LOCUS       sequence-21733-e         811 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Brzovic PS, Lissounov A, Christensen DE, Hoyt DW, Klevit RE
  TITLE     A UbcH5/ubiquitin noncovalent complex is required for processive 
            BRCA1-directed ubiquitination.
  JOURNAL   Mol Cell. 2006 Mar 17. 21(6):873-80.
  PUBMED    16543155
REFERENCE   2  (bases 1 to 811)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2006 Mar 17. 21(6):873-80."
COMMENT     Sequence Label: UbcH5c WT pET28a
FEATURES             Location/Qualifiers
     source          1..811
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             497..514
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(567..585)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      581..628
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(752..771)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 aggagatata ccatggcgct gaaacggatt aataaggaac ttagtgattt ggcccgtgac
       61 cctccagcac aatgttctgc aggtccagtt ggggatgata tgtttcattg gcaagccaca
      121 attatgggac ctaatgacag cccatatcaa ggcggtgtat tctttttgac aattcatttt
      181 cctacagact accccttcaa accacctaag gttgcattta caacaagaat ttatcatcca
      241 aatattaaca gtaatggcag catttgtctc gatattctaa gatcacagtg gtcgcctgct
      301 ttaacaattt ctaaagttct tttatccatt tgttcactgc tatgtgatcc aaacccagat
      361 gaccccctag tgccagagat tgcacggatc tataaaacag acagagataa gtacaacaga
      421 atatctcggg aatggactca gaagtatgcc atgtgatgct accttaaagt cagaataacc
      481 tgcattatag ctcgagcacc accaccacca ccactgagat ccggctgcta acaaagcccg
      541 aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc
      601 ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gattggcgaa
      661 tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
      721 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
      781 gccacgttcg ccggctttcc ccgtcaagct c
//
