LOCUS       sequence-217343-        4462 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  firefly luciferase (Fluc) reporter containing the 5'-UTR of GNB2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4462)
  AUTHORS   Ku YC, Lai MH, Lo CC, Cheng YC, Qiu JT, Tarn WY, Lai MC
  TITLE     DDX3 Participates in Translational Control of Inflammation Induced 
            by Infections and Injuries.
  JOURNAL   Mol Cell Biol. 2018 Dec 11;39(1). pii: MCB.00285-18. doi: 
            10.1128/MCB.00285-18. Print 2019 Jan 1.
  PUBMED    30373933
REFERENCE   2  (bases 1 to 4462)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol."; date: "2018-12-11"; volume: "39(1). pii: MCB.00285-18. doi";
            pages: " 10.1128/MCB.00285-18. Print 2019 Jan 1"
COMMENT     Sequence Label: pFL-SV40-GNB2 5'-UTR
FEATURES             Location/Qualifiers
     source          1..4462
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        62..419
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        62..253
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(85..105)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      270..405
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     regulatory      728..737
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             734..2386
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(800..818)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     polyA_signal    2417..2538
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2428..2447)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2482..2501
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        2671..2775
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2776..2844,2845..3636)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2994..3013)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3807..4395
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4296..4315
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agatctgcgc agcaccatgg cctgaaataa cctctgaaag aggaacttgg ttaggtacct
       61 tctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
      121 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
      181 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
      241 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
      301 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
      361 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
      421 agcttactgg cgggcgggat ccctccgctc tggggaggca gcgctggcgg cggggctggg
      481 gccactgagg aaatccatcc gcgccgccgc cgccgccgcc gccgccgccg ccgccgcctc
      541 cgccgcggag gaagacagcg ccgcccgcgc accgccagcg acctccgccg cagagtccca
      601 ccgccacagg cctcgggcca gcggccagga gctgcctccc ccagcccccg tcccgcggcc
      661 cccagccgcc cccaaccctg ccccacgggc ccggcgccaa gcttggcatt ccggtactgt
      721 tggtaaagcc accatggaag acgccaaaaa cataaagaaa ggcccggcgc cattctatcc
      781 gctggaagat ggaaccgctg gagagcaact gcataaggct atgaagagat acgccctggt
      841 tcctggaaca attgctttta cagatgcaca tatcgaggtg gacatcactt acgctgagta
      901 cttcgaaatg tccgttcggt tggcagaagc tatgaaacga tatgggctga atacaaatca
      961 cagaatcgtc gtatgcagtg aaaactctct tcaattcttt atgccggtgt tgggcgcgtt
     1021 atttatcgga gttgcagttg cgcccgcgaa cgacatttat aatgaacgtg aattgctcaa
     1081 cagtatgggc atttcgcagc ctaccgtggt gttcgtttcc aaaaaggggt tgcaaaaaat
     1141 tttgaacgtg caaaaaaagc tcccaatcat ccaaaaaatt attatcatgg attctaaaac
     1201 ggattaccag ggatttcagt cgatgtacac gttcgtcaca tctcatctac ctcccggttt
     1261 taatgaatac gattttgtgc cagagtcctt cgatagggac aagacaattg cactgatcat
     1321 gaactcctct ggatctactg gtctgcctaa aggtgtcgct ctgcctcata gaactgcctg
     1381 cgtgagattc tcgcatgcca gagatcctat ttttggcaat caaatcattc cggatactgc
     1441 gattttaagt gttgttccat tccatcacgg ttttggaatg tttactacac tcggatattt
     1501 gatatgtgga tttcgagtcg tcttaatgta tagatttgaa gaagagctgt ttctgaggag
     1561 ccttcaggat tacaagattc aaagtgcgct gctggtgcca accctattct ccttcttcgc
     1621 caaaagcact ctgattgaca aatacgattt atctaattta cacgaaattg cttctggtgg
     1681 cgctcccctc tctaaggaag tcggggaagc ggttgccaag aggttccatc tgccaggtat
     1741 caggcaagga tatgggctca ctgagactac atcagctatt ctgattacac ccgaggggga
     1801 tgataaaccg ggcgcggtcg gtaaagttgt tccatttttt gaagcgaagg ttgtggatct
     1861 ggataccggg aaaacgctgg gcgttaatca aagaggcgaa ctgtgtgtga gaggtcctat
     1921 gattatgtcc ggttatgtaa acaatccgga agcgaccaac gccttgattg acaaggatgg
     1981 atggctacat tctggagaca tagcttactg ggacgaagac gaacacttct tcatcgttga
     2041 ccgcctgaag tctctgatta agtacaaagg ctatcaggtg gctcccgctg aattggaatc
     2101 catcttgctc caacacccca acatcttcga cgcaggtgtc gcaggtcttc ccgacgatga
     2161 cgccggtgaa cttcccgccg ccgttgttgt tttggagcac ggaaagacga tgacggaaaa
     2221 agagatcgtg gattacgtcg ccagtcaagt aacaaccgcg aaaaagttgc gcggaggagt
     2281 tgtgtttgtg gacgaagtac cgaaaggtct taccggaaaa ctcgacgcaa gaaaaatcag
     2341 agagatcctc ataaaggcca agaagggcgg aaagatcgcc gtgtaattct agagcggccg
     2401 cttcgagcag acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag
     2461 tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata
     2521 agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg
     2581 gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtaa aatcgataag
     2641 gatccaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     2701 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     2761 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     2821 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     2881 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     2941 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     3001 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     3061 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     3121 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     3181 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     3241 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     3301 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     3361 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     3421 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     3481 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     3541 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     3601 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     3661 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     3721 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     3781 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     3841 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     3901 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     3961 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     4021 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     4081 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     4141 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     4201 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     4261 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     4321 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     4381 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     4441 ccttttgctc acatggctcg ac
//
