LOCUS       sequence-217352-        8964 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses hA3F-BE3 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8964)
  AUTHORS   Wang X, Li J, Wang Y, Yang B, Wei J, Wu J, Wang R, Huang X, Chen J, 
            Yang L
  TITLE     Efficient base editing in methylated regions with a human 
            APOBEC3A-Cas9 fusion.
  JOURNAL   Nat Biotechnol. 2018 Aug 20. pii: nbt.4198. doi: 10.1038/nbt.4198.
  PUBMED    30125268
REFERENCE   2  (bases 1 to 8964)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2018 Aug 20. pii: nbt.4198. doi: 10.1038/nbt.4198."
COMMENT     Sequence Label: pCMV-hA3F-BE3
FEATURES             Location/Qualifiers
     source          1..8964
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        96..475
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        476..679
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     630..650
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     721..740
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        721..739
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(1920..5318,5319..5321,5322..5918,5919,5920..5922,
                     5923..5924,5925..5927,5928..6020)
                     /codon_start=1
                     /product="Streptococcus pyogenes Cas9 endonuclease with the
                     D1135V/R1335Q/T1337R mutations"
                     /label=Cas9 VQR
                     /note="has an altered recognition specificity for the 
                     protospacer adjacent motif (PAM) (Kleinstiver et al., 
                     2015)"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             join(1920..1943,1944..1946,1947..6020)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             6033..6281
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             6294..6314
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6323..6340
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6363..6380)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6369..6593
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6664..6680)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6664..6680)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6677..6699)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6688..6704
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6712..6718,6719..6736,6737..6742))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6757..6778
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6895..6912)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7066..7654)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7146..7165)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7825..8616,8617..8685))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8448..8467
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8686..8790)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8869..8888)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 aacaaggcaa ggcttgaccg acaattgcat gaagaatctg cttagggtta ggcgttttgc
       61 gctgcttcgc gatgtacggg ccagatatac gcgttgacat tgattattga ctagttatta
      121 atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata
      181 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat
      241 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga
      301 gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc
      361 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt
      421 atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat
      481 gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag
      541 tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc
      601 aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga
      661 ggtctatata agcagagctg gtttagtgaa ccgtcagatc cgctagagat ccgcggccgc
      721 taatacgact cactataggg agagccgcca ccatgaagcc tcacttcaga aacacagtgg
      781 agcgaatgta tcgagacaca ttctcctaca acttttataa tagacccatc ctttctcgtc
      841 ggaataccgt ctggctgtgc tacgaagtga aaacaaaggg tccctcaagg ccccgtttgg
      901 acgcaaagat ctttcgaggc caggtgtatt cccagcctga gcaccacgca gaaatgtgct
      961 tcctctcttg gttctgtggc aaccagctgc ctgcttacaa gtgtttccag atcacctggt
     1021 ttgtatcctg gaccccctgc ccggactgtg tggcgaagct ggccgaattc ctggctgagc
     1081 accccaatgt caccctgacc atctccgccg cccgcctcta ctactactgg gaaagagatt
     1141 accgaagggc gctctgcagg ctgagtcagg caggggcccg cgtgaagatt atggacgatg
     1201 aagaatttgc atactgctgg gaaaactttg tgtacagtga aggtcagcca ttcatgcctt
     1261 ggtacaaatt cgatgacaat tatgcattcc tgcaccgcac gctaaaggag attctcagaa
     1321 acccgatgga ggcaatgtat ccacacatat tctacttcca ctttaaaaac ctacgcaaag
     1381 cctatggtcg gaacgaaagc tggctgtgct tcaccatgga agttgtaaag caccactcac
     1441 ctgtctcctg gaagaggggc gtcttccgaa accaggtgga tcctgagacc cattgtcatg
     1501 cagaaaggtg cttcctctct tggttctgtg acgacatact gtctcctaac acaaactacg
     1561 aggtcacctg gtacacatct tggagccctt gcccagagtg tgcaggggag gtggccgagt
     1621 tcctggccag gcacagcaac gtgaatctca ccatcttcac cgcccgcctc tactacttct
     1681 gggatacaga ttaccaggag gggctccgca gcctgagtca ggaaggggcc tccgtggaga
     1741 tcatgggcta caaagatttt aaatattgtt gggaaaactt tgtgtacaat gatgatgagc
     1801 cattcaagcc ttggaaagga ctaaaataca actttctatt cctggacagc aagctgcagg
     1861 agattctcga gagcggcagc gagactcccg ggacctcaga gtccgccaca cccgaaagtg
     1921 ataaaaagta ttctattggt ttagccatcg gcactaattc cgttggatgg gctgtcataa
     1981 ccgatgaata caaagtacct tcaaagaaat ttaaggtgtt ggggaacaca gaccgtcatt
     2041 cgattaaaaa gaatcttatc ggtgccctcc tattcgatag tggcgaaacg gcagaggcga
     2101 ctcgcctgaa acgaaccgct cggagaaggt atacacgtcg caagaaccga atatgttact
     2161 tacaagaaat ttttagcaat gagatggcca aagttgacga ttctttcttt caccgtttgg
     2221 aagagtcctt ccttgtcgaa gaggacaaga aacatgaacg gcaccccatc tttggaaaca
     2281 tagtagatga ggtggcatat catgaaaagt acccaacgat ttatcacctc agaaaaaagc
     2341 tagttgactc aactgataaa gcggacctga ggttaatcta cttggctctt gcccatatga
     2401 taaagttccg tgggcacttt ctcattgagg gtgatctaaa tccggacaac tcggatgtcg
     2461 acaaactgtt catccagtta gtacaaacct ataatcagtt gtttgaagag aaccctataa
     2521 atgcaagtgg cgtggatgcg aaggctattc ttagcgcccg cctctctaaa tcccgacggc
     2581 tagaaaacct gatcgcacaa ttacccggag agaagaaaaa tgggttgttc ggtaacctta
     2641 tagcgctctc actaggcctg acaccaaatt ttaagtcgaa cttcgactta gctgaagatg
     2701 ccaaattgca gcttagtaag gacacgtacg atgacgatct cgacaatcta ctggcacaaa
     2761 ttggagatca gtatgcggac ttatttttgg ctgccaaaaa ccttagcgat gcaatcctcc
     2821 tatctgacat actgagagtt aatactgaga ttaccaaggc gccgttatcc gcttcaatga
     2881 tcaaaaggta cgatgaacat caccaagact tgacacttct caaggcccta gtccgtcagc
     2941 aactgcctga gaaatataag gaaatattct ttgatcagtc gaaaaacggg tacgcaggtt
     3001 atattgacgg cggagcgagt caagaggaat tctacaagtt tatcaaaccc atattagaga
     3061 agatggatgg gacggaagag ttgcttgtaa aactcaatcg cgaagatcta ctgcgaaagc
     3121 agcggacttt cgacaacggt agcattccac atcaaatcca cttaggcgaa ttgcatgcta
     3181 tacttagaag gcaggaggat ttttatccgt tcctcaaaga caatcgtgaa aagattgaga
     3241 aaatcctaac ctttcgcata ccttactatg tgggacccct ggcccgaggg aactctcggt
     3301 tcgcatggat gacaagaaag tccgaagaaa cgattactcc atggaatttt gaggaagttg
     3361 tcgataaagg tgcgtcagct caatcgttca tcgagaggat gaccaacttt gacaagaatt
     3421 taccgaacga aaaagtattg cctaagcaca gtttacttta cgagtatttc acagtgtaca
     3481 atgaactcac gaaagttaag tatgtcactg agggcatgcg taaacccgcc tttctaagcg
     3541 gagaacagaa gaaagcaata gtagatctgt tattcaagac caaccgcaaa gtgacagtta
     3601 agcaattgaa agaggactac tttaagaaaa ttgaatgctt cgattctgtc gagatctccg
     3661 gggtagaaga tcgatttaat gcgtcacttg gtacgtatca tgacctccta aagataatta
     3721 aagataagga cttcctggat aacgaagaga atgaagatat cttagaagat atagtgttga
     3781 ctcttaccct ctttgaagat cgggaaatga ttgaggaaag actaaaaaca tacgctcacc
     3841 tgttcgacga taaggttatg aaacagttaa agaggcgtcg ctatacgggc tggggacgat
     3901 tgtcgcggaa acttatcaac gggataagag acaagcaaag tggtaaaact attctcgatt
     3961 ttctaaagag cgacggcttc gccaatagga actttatgca gctgatccat gatgactctt
     4021 taaccttcaa agaggatata caaaaggcac aggtttccgg acaaggggac tcattgcacg
     4081 aacatattgc gaatcttgct ggttcgccag ccatcaaaaa gggcatactc cagacagtca
     4141 aagtagtgga tgagctagtt aaggtcatgg gacgtcacaa accggaaaac attgtaatcg
     4201 agatggcacg cgaaaatcaa acgactcaga aggggcaaaa aaacagtcga gagcggatga
     4261 agagaataga agagggtatt aaagaactgg gcagccagat cttaaaggag catcctgtgg
     4321 aaaataccca attgcagaac gagaaacttt acctctatta cctacaaaat ggaagggaca
     4381 tgtatgttga tcaggaactg gacataaacc gtttatctga ttacgacgtc gatcacattg
     4441 taccccaatc ctttttgaag gacgattcaa tcgacaataa agtgcttaca cgctcggata
     4501 agaaccgagg gaaaagtgac aatgttccaa gcgaggaagt cgtaaagaaa atgaagaact
     4561 attggcggca gctcctaaat gcgaaactga taacgcaaag aaagttcgat aacttaacta
     4621 aagctgagag gggtggcttg tctgaacttg acaaggccgg atttattaaa cgtcagctcg
     4681 tggaaacccg ccaaatcaca aagcatgttg cacagatact agattcccga atgaatacga
     4741 aatacgacga gaacgataag ctgattcggg aagtcaaagt aatcacttta aagtcaaaat
     4801 tggtgtcgga cttcagaaag gattttcaat tctataaagt tagggagata aataactacc
     4861 accatgcgca cgacgcttat cttaatgccg tcgtagggac cgcactcatt aagaaatacc
     4921 cgaagctaga aagtgagttt gtgtatggtg attacaaagt ttatgacgtc cgtaagatga
     4981 tcgcgaaaag cgaacaggag ataggcaagg ctacagccaa atacttcttt tattctaaca
     5041 ttatgaattt ctttaagacg gaaatcactc tggcaaacgg agagatacgc aaacgacctt
     5101 taattgaaac caatggggag acaggtgaaa tcgtatggga taagggccgg gacttcgcga
     5161 cggtgagaaa agttttgtcc atgccccaag tcaacatagt aaagaaaact gaggtgcaga
     5221 ccggagggtt ttcaaaggaa tcgattcttc caaaaaggaa tagtgataag ctcatcgctc
     5281 gtaaaaagga ctgggacccg aaaaagtacg gtggcttcga tagccctaca gttgcctatt
     5341 ctgtcctagt agtggcaaaa gttgagaagg gaaaatccaa gaaactgaag tcagtcaaag
     5401 aattattggg gataacgatt atggagcgct cgtcttttga aaagaacccc atcgacttcc
     5461 ttgaggcgaa aggttacaag gaagtaaaaa aggatctcat aattaaacta ccaaagtata
     5521 gtctgtttga gttagaaaat ggccgaaaac ggatgttggc tagcgccgga gagcttcaaa
     5581 aggggaacga actcgcacta ccgtctaaat acgtgaattt cctgtattta gcgtcccatt
     5641 acgagaagtt gaaaggttca cctgaagata acgaacagaa gcaacttttt gttgagcagc
     5701 acaaacatta tctcgacgaa atcatagagc aaatttcgga attcagtaag agagtcatcc
     5761 tagctgatgc caatctggac aaagtattaa gcgcatacaa caagcacagg gataaaccca
     5821 tacgtgagca ggcggaaaat attatccatt tgtttactct taccaacctc ggcgctccag
     5881 ccgcattcaa gtattttgac acaacgatag atcgcaaacg atacacttct accaaggagg
     5941 tgctagacgc gacactgatt caccaatcca tcacgggatt atatgaaact cggatagatt
     6001 tgtcacagct tgggggtgac tctggtggtt ctactaatct gtcagatatt attgaaaagg
     6061 agaccggtaa gcaactggtt atccaggaat ccatcctcat gctcccagag gaggtggaag
     6121 aagtcattgg gaacaagccg gaaagcgata tactcgtgca caccgcctac gacgagagca
     6181 ccgacgagaa tgtcatgctt ctgactagcg acgcccctga atacaagcct tgggctctgg
     6241 tcatacagga tagcaacggt gagaacaaga ttaagatgct ctctggtggt tctcccaaga
     6301 agaagaggaa agtctaaccg gtcatcatca ccatcaccat tgagtttaaa cccgctgatc
     6361 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc
     6421 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc
     6481 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg
     6541 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga
     6601 ggcggaaaga accagctggg gctcgatacc gtcgacctct agctagagct tggcgtaatc
     6661 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     6721 agccggaagc ataaagtgta aagcctaggg tgcctaatga gtgagctaac tcacattaat
     6781 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     6841 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     6901 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     6961 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     7021 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     7081 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     7141 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     7201 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     7261 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     7321 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     7381 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     7441 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     7501 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     7561 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     7621 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     7681 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     7741 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     7801 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     7861 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     7921 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     7981 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     8041 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     8101 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     8161 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     8221 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     8281 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     8341 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     8401 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     8461 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     8521 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     8581 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     8641 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     8701 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     8761 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
     8821 cgacggatcg ggagatcgat ctcccgatcc cctagggtcg actctcagta caatctgctc
     8881 tgatgccgca tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta
     8941 gtgcgcgagc aaaatttaag ctac
//
