LOCUS       sequence-217362-        6261 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses worm Myc-tagged MPS-1  in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6261)
  AUTHORS   Wang Y, Sesti F
  TITLE     Molecular mechanisms underlying KVS-1-MPS-1 complex assembly.
  JOURNAL   Biophys J. 2007 Nov 1;93(9):3083-91. doi: 
            10.1529/biophysj.107.110320. Epub 2007 Jun 29.
  PUBMED    17604313
REFERENCE   2  (bases 1 to 6261)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1529/biophysj.107.110320"; journalName: "Biophys J"; date: 
            "2007-11-1- 1"; volume: "93"; issue: "9"; pages: "3083-91"
COMMENT     Sequence Label: MPS-1-cmyc
FEATURES             Location/Qualifiers
     source          1..6261
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          161..293
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     276..295
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        338..356
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1141..1170
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     polyA_signal    1236..1357
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1247..1266)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1301..1320
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      1543..1998
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1630..1649)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1840..1861
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2115..2472
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        2115..2306
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(2138..2158)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      2323..2458
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2523..3317
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWMLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2577..2596)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3187..3206
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    3381..3429
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(3470..3489)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3589..3611
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3649..3667)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3735..3839
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3840..3908,3909..4700)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFSVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4058..4077)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4871..5459
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5360..5379
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        5670..6049
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6050..6261
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6212..6232
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     join(6258..6261,1..21)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 cgtttagtga accgtcagat cactagaagc tttattgcgg tagtttatca cagttaaatt
       61 gctaacgcag tcagtgcttc tgacacaaca gtctcgaact taagctgcag tgactctctt
      121 aaggtagcct tgcagaagtt ggtcgtgagg cactgggcag gtaagtatca aggttacaag
      181 acaggtttaa ggagaccaat agaaactggg cttgtcgaga cagagaagac tcttgcgttt
      241 ctgataggca cctattggtc ttactgacat ccactttgcc tttctctcca caggtgtcca
      301 ctcccagttc aattacagct cttaaggcta gagtacttaa tacgactcac tataggctag
      361 cctcgagcca ccatgcacaa aaatatcagt cccacgtgcc aagaaggcat tcgagaagca
      421 tgcatcactt tgaaaaatgc ttgtgatccc caagtacttg acaaggctgc aatccgaatg
      481 agagagtacg aaaatgagtt cggtcgaata ctttatacaa tatcaatttt aataatgttc
      541 tcatttgtca ttattctcct aatggtacga tcaattcgga gaacacaatc aacagttgag
      601 atggattcgc tccttgatgc tatgcgaatt cgagaagaat tagaaataca ggaacgtaaa
      661 agaagacgat tgatgagagc aaaaacccag gtgactgcat ggctcgtgaa caaaaacaaa
      721 gagaaaggac cagaaaagcg aaaagatagc gaatggaagc cacttccaaa tggaacaaga
      781 ccaagaggac attacagcat atcaacagtc acatcagaca ttccggagat tgtagtaagt
      841 gctgacgact gcatacattc tgattttcca aatcgtccac atactcctgc aatctcaatg
      901 atttacgatt ttggaattgc gtcaccggat ttaatagagc ccgatagccg taagccatca
      961 atttcctctt caacagcaat tcccatgtca tcttcgtcat cttcatcaat gaaatcattg
     1021 attgaaccaa atatgaattc aataaaatca aatcctcgaa catattcgct tgacacaaac
     1081 gcctccacgt catcgagaac tcctcgtgtt gattgtgacg ataaatcatg cttagacgtg
     1141 gaacaaaaac ttatttctga agaagatctg tagtctagag tcgacccggg cggccgcttc
     1201 cctttagtga gggttaatgc ttcgagcaga catgataaga tacattgatg agtttggaca
     1261 aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc
     1321 tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt
     1381 tatgtttcag gttcaggggg agatgtggga ggttttttaa agcaagtaaa acctctacaa
     1441 atgtggtaaa atccgataag gatcgatccg ggctggcgta atagcgaaga ggcccgcacc
     1501 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggacgcgccc tgtagcggcg
     1561 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     1621 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     1681 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     1741 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     1801 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     1861 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
     1921 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
     1981 tattaacgct tacaatttcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc
     2041 acaccgcata cgcggatctg cgcagcacca tggcctgaaa taacctctga aagaggaact
     2101 tggttaggta ccttctgagg cggaaagaac cagctgtgga atgtgtgtca gttagggtgt
     2161 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     2221 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     2281 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
     2341 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     2401 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     2461 ggcttttgca aaaagcttga ttcttctgac acaacagtct cgaacttaag gctagagcca
     2521 ccatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat
     2581 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt
     2641 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac
     2701 tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg
     2761 tgctcgacgt tgtcactgaa gcgggaaggg actggatgct attgggcgaa gtgccggggc
     2821 aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa
     2881 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc
     2941 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg
     3001 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg
     3061 acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa
     3121 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg
     3181 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct
     3241 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc
     3301 ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa
     3361 cctgccatca cgatggccgc aataaaatat ctttattttc attacatctg tgtgttggtt
     3421 ttttgtgtga atcgatagcg ataaggatcc gcgtatggtg cactctcagt acaatctgct
     3481 ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac
     3541 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
     3601 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac
     3661 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
     3721 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     3781 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     3841 tgagtattca acatttcagt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     3901 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     3961 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     4021 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     4081 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     4141 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     4201 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     4261 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     4321 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     4381 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     4441 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     4501 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     4561 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     4621 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     4681 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     4741 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     4801 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     4861 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     4921 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     4981 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     5041 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     5101 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     5161 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     5221 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     5281 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     5341 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     5401 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     5461 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgg
     5521 ctcgacagat cttcaatatt ggccattagc catattattc attggttata tagcataaat
     5581 caatattggc tattggccat tgcatacgtt gtatctatat cataatatgt acatttatat
     5641 tggctcatgt ccaatatgac cgccatgttg gcattgatta ttgactagtt attaatagta
     5701 atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac
     5761 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac
     5821 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt
     5881 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagtc cgccccctat
     5941 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttacggga
     6001 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt
     6061 ttggcagtac accaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca
     6121 ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg
     6181 tcgtaacaac tgcgatcgcc cgtcccgttg acgcaaatgg gcggtaggcg tgtacggtgg
     6241 gaggtctata taagcagagc t
//
