LOCUS       sequence-217363-        8439 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses mAID-BE3 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8439)
  AUTHORS   Wang X, Li J, Wang Y, Yang B, Wei J, Wu J, Wang R, Huang X, Chen J, 
            Yang L
  TITLE     Efficient base editing in methylated regions with a human 
            APOBEC3A-Cas9 fusion.
  JOURNAL   Nat Biotechnol. 2018 Aug 20. pii: nbt.4198. doi: 10.1038/nbt.4198.
  PUBMED    30125268
REFERENCE   2  (bases 1 to 8439)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2018 Aug 20. pii: nbt.4198. doi: 10.1038/nbt.4198."
COMMENT     Sequence Label: pCMV-mAID-BE3
FEATURES             Location/Qualifiers
     source          1..8439
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        171..550
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        551..754
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     705..725
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     796..815
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        796..814
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      822..831
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1470..4868,4869..4871,4872..5468,5469,5470..5472,
                     5473..5474,5475..5477,5478..5570)
                     /codon_start=1
                     /product="Streptococcus pyogenes Cas9 endonuclease with the
                     D1135V/R1335Q/T1337R mutations"
                     /label=Cas9 VQR
                     /note="has an altered recognition specificity for the 
                     protospacer adjacent motif (PAM) (Kleinstiver et al., 
                     2015)"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             join(1470..1493,1494..1496,1497..5570)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5583..5831
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             5844..5864
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5873..5890
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5913..5930)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5919..6143
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6214..6230)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6214..6230)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6227..6249)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6238..6254
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6262..6268,6269..6286,6287..6292))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6307..6328
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6445..6462)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6616..7204)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6696..6715)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7375..8166,8167..8235))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7998..8017
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8236..8340)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8419..8438)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag
       61 caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga atctgcttag
      121 ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt gacattgatt
      181 attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
      241 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
      301 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
      361 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
      421 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
      481 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
      541 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
      601 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
      661 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
      721 gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta
      781 gagatccgcg gccgctaata cgactcacta tagggagagc cgccaccatg gacagccttc
      841 tgatgaagca aaagaagttt ctttaccatt tcaaaaatgt ccgctgggcc aagggacggc
      901 atgagaccta cctctgctac gtggtgaaga ggagagatag tgccacctcc tgctcactgg
      961 acttcggcca ccttcgcaac aagtctggct gccacgtgga attgttgttc ctacgctaca
     1021 tctcagactg ggacctggac ccgggccggt gttaccgcgt cacctggttc acctcctgga
     1081 gcccgtgcta tgactgtgcc cggcacgtgg ctgagtttct gagatggaac cctaacctca
     1141 gcctgaggat tttcaccgcg cgcctctact tctgtgaaga ccgcaaggct gagcctgagg
     1201 ggctgcggag actgcaccgc gctggggtcc agatcgggat catgaccttc aaagactatt
     1261 tttactgctg gaatacattt gtagaaaatc gtgaaagaac tttcaaagcc tgggaagggc
     1321 tacatgaaaa ttctgtccgg ctaaccagac aacttcggcg catccttttg cccttgtacg
     1381 aagtcgatga cttgcgagat gcatttcgta tgttgggatt tagcggcagc gagactcccg
     1441 ggacctcaga gtccgccaca cccgaaagtg ataaaaagta ttctattggt ttagccatcg
     1501 gcactaattc cgttggatgg gctgtcataa ccgatgaata caaagtacct tcaaagaaat
     1561 ttaaggtgtt ggggaacaca gaccgtcatt cgattaaaaa gaatcttatc ggtgccctcc
     1621 tattcgatag tggcgaaacg gcagaggcga ctcgcctgaa acgaaccgct cggagaaggt
     1681 atacacgtcg caagaaccga atatgttact tacaagaaat ttttagcaat gagatggcca
     1741 aagttgacga ttctttcttt caccgtttgg aagagtcctt ccttgtcgaa gaggacaaga
     1801 aacatgaacg gcaccccatc tttggaaaca tagtagatga ggtggcatat catgaaaagt
     1861 acccaacgat ttatcacctc agaaaaaagc tagttgactc aactgataaa gcggacctga
     1921 ggttaatcta cttggctctt gcccatatga taaagttccg tgggcacttt ctcattgagg
     1981 gtgatctaaa tccggacaac tcggatgtcg acaaactgtt catccagtta gtacaaacct
     2041 ataatcagtt gtttgaagag aaccctataa atgcaagtgg cgtggatgcg aaggctattc
     2101 ttagcgcccg cctctctaaa tcccgacggc tagaaaacct gatcgcacaa ttacccggag
     2161 agaagaaaaa tgggttgttc ggtaacctta tagcgctctc actaggcctg acaccaaatt
     2221 ttaagtcgaa cttcgactta gctgaagatg ccaaattgca gcttagtaag gacacgtacg
     2281 atgacgatct cgacaatcta ctggcacaaa ttggagatca gtatgcggac ttatttttgg
     2341 ctgccaaaaa ccttagcgat gcaatcctcc tatctgacat actgagagtt aatactgaga
     2401 ttaccaaggc gccgttatcc gcttcaatga tcaaaaggta cgatgaacat caccaagact
     2461 tgacacttct caaggcccta gtccgtcagc aactgcctga gaaatataag gaaatattct
     2521 ttgatcagtc gaaaaacggg tacgcaggtt atattgacgg cggagcgagt caagaggaat
     2581 tctacaagtt tatcaaaccc atattagaga agatggatgg gacggaagag ttgcttgtaa
     2641 aactcaatcg cgaagatcta ctgcgaaagc agcggacttt cgacaacggt agcattccac
     2701 atcaaatcca cttaggcgaa ttgcatgcta tacttagaag gcaggaggat ttttatccgt
     2761 tcctcaaaga caatcgtgaa aagattgaga aaatcctaac ctttcgcata ccttactatg
     2821 tgggacccct ggcccgaggg aactctcggt tcgcatggat gacaagaaag tccgaagaaa
     2881 cgattactcc atggaatttt gaggaagttg tcgataaagg tgcgtcagct caatcgttca
     2941 tcgagaggat gaccaacttt gacaagaatt taccgaacga aaaagtattg cctaagcaca
     3001 gtttacttta cgagtatttc acagtgtaca atgaactcac gaaagttaag tatgtcactg
     3061 agggcatgcg taaacccgcc tttctaagcg gagaacagaa gaaagcaata gtagatctgt
     3121 tattcaagac caaccgcaaa gtgacagtta agcaattgaa agaggactac tttaagaaaa
     3181 ttgaatgctt cgattctgtc gagatctccg gggtagaaga tcgatttaat gcgtcacttg
     3241 gtacgtatca tgacctccta aagataatta aagataagga cttcctggat aacgaagaga
     3301 atgaagatat cttagaagat atagtgttga ctcttaccct ctttgaagat cgggaaatga
     3361 ttgaggaaag actaaaaaca tacgctcacc tgttcgacga taaggttatg aaacagttaa
     3421 agaggcgtcg ctatacgggc tggggacgat tgtcgcggaa acttatcaac gggataagag
     3481 acaagcaaag tggtaaaact attctcgatt ttctaaagag cgacggcttc gccaatagga
     3541 actttatgca gctgatccat gatgactctt taaccttcaa agaggatata caaaaggcac
     3601 aggtttccgg acaaggggac tcattgcacg aacatattgc gaatcttgct ggttcgccag
     3661 ccatcaaaaa gggcatactc cagacagtca aagtagtgga tgagctagtt aaggtcatgg
     3721 gacgtcacaa accggaaaac attgtaatcg agatggcacg cgaaaatcaa acgactcaga
     3781 aggggcaaaa aaacagtcga gagcggatga agagaataga agagggtatt aaagaactgg
     3841 gcagccagat cttaaaggag catcctgtgg aaaataccca attgcagaac gagaaacttt
     3901 acctctatta cctacaaaat ggaagggaca tgtatgttga tcaggaactg gacataaacc
     3961 gtttatctga ttacgacgtc gatcacattg taccccaatc ctttttgaag gacgattcaa
     4021 tcgacaataa agtgcttaca cgctcggata agaaccgagg gaaaagtgac aatgttccaa
     4081 gcgaggaagt cgtaaagaaa atgaagaact attggcggca gctcctaaat gcgaaactga
     4141 taacgcaaag aaagttcgat aacttaacta aagctgagag gggtggcttg tctgaacttg
     4201 acaaggccgg atttattaaa cgtcagctcg tggaaacccg ccaaatcaca aagcatgttg
     4261 cacagatact agattcccga atgaatacga aatacgacga gaacgataag ctgattcggg
     4321 aagtcaaagt aatcacttta aagtcaaaat tggtgtcgga cttcagaaag gattttcaat
     4381 tctataaagt tagggagata aataactacc accatgcgca cgacgcttat cttaatgccg
     4441 tcgtagggac cgcactcatt aagaaatacc cgaagctaga aagtgagttt gtgtatggtg
     4501 attacaaagt ttatgacgtc cgtaagatga tcgcgaaaag cgaacaggag ataggcaagg
     4561 ctacagccaa atacttcttt tattctaaca ttatgaattt ctttaagacg gaaatcactc
     4621 tggcaaacgg agagatacgc aaacgacctt taattgaaac caatggggag acaggtgaaa
     4681 tcgtatggga taagggccgg gacttcgcga cggtgagaaa agttttgtcc atgccccaag
     4741 tcaacatagt aaagaaaact gaggtgcaga ccggagggtt ttcaaaggaa tcgattcttc
     4801 caaaaaggaa tagtgataag ctcatcgctc gtaaaaagga ctgggacccg aaaaagtacg
     4861 gtggcttcga tagccctaca gttgcctatt ctgtcctagt agtggcaaaa gttgagaagg
     4921 gaaaatccaa gaaactgaag tcagtcaaag aattattggg gataacgatt atggagcgct
     4981 cgtcttttga aaagaacccc atcgacttcc ttgaggcgaa aggttacaag gaagtaaaaa
     5041 aggatctcat aattaaacta ccaaagtata gtctgtttga gttagaaaat ggccgaaaac
     5101 ggatgttggc tagcgccgga gagcttcaaa aggggaacga actcgcacta ccgtctaaat
     5161 acgtgaattt cctgtattta gcgtcccatt acgagaagtt gaaaggttca cctgaagata
     5221 acgaacagaa gcaacttttt gttgagcagc acaaacatta tctcgacgaa atcatagagc
     5281 aaatttcgga attcagtaag agagtcatcc tagctgatgc caatctggac aaagtattaa
     5341 gcgcatacaa caagcacagg gataaaccca tacgtgagca ggcggaaaat attatccatt
     5401 tgtttactct taccaacctc ggcgctccag ccgcattcaa gtattttgac acaacgatag
     5461 atcgcaaacg atacacttct accaaggagg tgctagacgc gacactgatt caccaatcca
     5521 tcacgggatt atatgaaact cggatagatt tgtcacagct tgggggtgac tctggtggtt
     5581 ctactaatct gtcagatatt attgaaaagg agaccggtaa gcaactggtt atccaggaat
     5641 ccatcctcat gctcccagag gaggtggaag aagtcattgg gaacaagccg gaaagcgata
     5701 tactcgtgca caccgcctac gacgagagca ccgacgagaa tgtcatgctt ctgactagcg
     5761 acgcccctga atacaagcct tgggctctgg tcatacagga tagcaacggt gagaacaaga
     5821 ttaagatgct ctctggtggt tctcccaaga agaagaggaa agtctaaccg gtcatcatca
     5881 ccatcaccat tgagtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc
     5941 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt
     6001 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct
     6061 ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc
     6121 tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg gctcgatacc
     6181 gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     6241 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctaggg
     6301 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     6361 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     6421 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     6481 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     6541 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     6601 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     6661 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     6721 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     6781 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     6841 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     6901 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     6961 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     7021 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     7081 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     7141 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     7201 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     7261 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     7321 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     7381 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     7441 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     7501 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     7561 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     7621 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     7681 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     7741 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     7801 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     7861 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     7921 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     7981 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     8041 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     8101 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     8161 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     8221 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     8281 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     8341 cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatcgat ctcccgatcc
     8401 cctagggtcg actctcagta caatctgctc tgatgccgc
//
