LOCUS       sequence-217367-        1710 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BTK Type 2 promoter for golden gate assembly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1710)
  AUTHORS   Leonard SP, Perutka J, Powell JE, Geng P, Richhart DD, Byrom M, Kar 
            S, Davies BW, Ellington AD, Moran NA, Barrick JE
  TITLE     Genetic Engineering of Bee Gut Microbiome Bacteria with a Toolkit 
            for Modular Assembly of Broad-Host-Range Plasmids.
  JOURNAL   ACS Synth Biol. 2018 May 18;7(5):1279-1290. doi: 
            10.1021/acssynbio.7b00399. Epub 2018 Apr 13.
  PUBMED    29608282
REFERENCE   2  (bases 1 to 1710)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.7b00399"; journalName: "ACS Synth Biol"; date: 
            "2018-05-18- 18"; volume: "7"; issue: "5"; pages: "1279-1290"
COMMENT     Sequence Label: pBTK102
FEATURES             Location/Qualifiers
     source          1..1710
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        12..30
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      complement(73..107)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(178..837)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     752..771
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1019..1607
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1508..1527
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ggtctcaaac gtaatacgac tcactatagg atacagaaac agaggagata ttacatatgt
       61 gagaccagac caataaaaaa cgcccggcgg caaccgagcg ttctgaacaa atccagatgg
      121 agttctgagg tcattactgg atctatcaac aggagtccaa gcgagctcga tatcaaatta
      181 cgccccgccc tgccactcat cgcagtactg ttgtaattca ttaagcattc tgccgacatg
      241 gaagccatca caaacggcat gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc
      301 ttgcgtataa tatttgccca tggtgaaaac gggggcgaag aagttgtcca tattggccac
      361 gtttaaatca aaactggtga aactcaccca gggattggct gaaacgaaaa acatattctc
      421 aataaaccct ttagggaaat aggccaggtt ttcaccgtaa cacgccacat cttgcgaata
      481 tatgtgtaga aactgccgga aatcgtcgtg gtattcactc cagagcgatg aaaacgtttc
      541 agtttgctca tggaaaacgg tgtaacaagg gtgaacacta tcccatatca ccagctcacc
      601 gtctttcatt gccatacgaa attccggatg agcattcatc aggcgggcaa gaatgtgaat
      661 aaaggccgga taaaacttgt gcttattttt ctttacggtc tttaaaaagg ccgtaatatc
      721 cagctgaacg gtctggttat aggtacattg agcaactgac tgaaatgcct caaaatgttc
      781 tttacgatgc cattgggata tatcaacggt ggtatatcca gtgatttttt tctccatttt
      841 agcttcctta gctcctgaaa atctcgataa ctcaaaaaat acgcccggta gtgatcttat
      901 ttcattatgg tgaaagttgg aacctcttac gtgcccgatc aatcatgacc aaaatccctt
      961 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     1021 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     1081 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     1141 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     1201 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     1261 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     1321 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     1381 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     1441 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     1501 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     1561 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     1621 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     1681 tatcccctga ttctgtggat aaccgtagtc
//
