LOCUS       sequence-217368-        1751 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BTK Type 2 promoter for golden gate assembly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1751)
  AUTHORS   Leonard SP, Perutka J, Powell JE, Geng P, Richhart DD, Byrom M, Kar 
            S, Davies BW, Ellington AD, Moran NA, Barrick JE
  TITLE     Genetic Engineering of Bee Gut Microbiome Bacteria with a Toolkit 
            for Modular Assembly of Broad-Host-Range Plasmids.
  JOURNAL   ACS Synth Biol. 2018 May 18;7(5):1279-1290. doi: 
            10.1021/acssynbio.7b00399. Epub 2018 Apr 13.
  PUBMED    29608282
REFERENCE   2  (bases 1 to 1751)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.7b00399"; journalName: "ACS Synth Biol"; date: 
            "2018-05-18- 18"; volume: "7"; issue: "5"; pages: "1279-1290"
COMMENT     Sequence Label: pBTK110
FEATURES             Location/Qualifiers
     source          1..1751
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(158..192)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(263..922)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     837..856
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1104..1692
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1593..1612
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gttctttcct gcgttatccc ctgattctgt ggataaccgt agtcggtctc aaacgcatgt
       61 gggagtttat tcttgacaca gatatttccg gatgatataa taactgagta ctgttataca
      121 gaaacagagg agatattaca tatgtgagac cagaccaata aaaaacgccc ggcggcaacc
      181 gagcgttctg aacaaatcca gatggagttc tgaggtcatt actggatcta tcaacaggag
      241 tccaagcgag ctcgatatca aattacgccc cgccctgcca ctcatcgcag tactgttgta
      301 attcattaag cattctgccg acatggaagc catcacaaac ggcatgatga acctgaatcg
      361 ccagcggcat cagcaccttg tcgccttgcg tataatattt gcccatggtg aaaacggggg
      421 cgaagaagtt gtccatattg gccacgttta aatcaaaact ggtgaaactc acccagggat
      481 tggctgaaac gaaaaacata ttctcaataa accctttagg gaaataggcc aggttttcac
      541 cgtaacacgc cacatcttgc gaatatatgt gtagaaactg ccggaaatcg tcgtggtatt
      601 cactccagag cgatgaaaac gtttcagttt gctcatggaa aacggtgtaa caagggtgaa
      661 cactatccca tatcaccagc tcaccgtctt tcattgccat acgaaattcc ggatgagcat
      721 tcatcaggcg ggcaagaatg tgaataaagg ccggataaaa cttgtgctta tttttcttta
      781 cggtctttaa aaaggccgta atatccagct gaacggtctg gttataggta cattgagcaa
      841 ctgactgaaa tgcctcaaaa tgttctttac gatgccattg ggatatatca acggtggtat
      901 atccagtgat ttttttctcc attttagctt ccttagctcc tgaaaatctc gataactcaa
      961 aaaatacgcc cggtagtgat cttatttcat tatggtgaaa gttggaacct cttacgtgcc
     1021 cgatcaatca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     1081 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     1141 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     1201 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     1261 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     1321 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     1381 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     1441 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     1501 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     1561 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     1621 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     1681 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     1741 tttgctcaca t
//
