LOCUS       sequence-217376-        5628 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses aa397 to C-terminus of PhiC31 integrase fused to FKBP CID.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5628)
  AUTHORS   Weinberg BH, Cho JH, Agarwal Y, Pham NTH, Caraballo LD, Walkosz M, 
            Ortega C, Trexler M, Tague N, Law B, Benman WKJ, Letendre J, Beal J,
            Wong WW
  TITLE     High-performance chemical- and light-inducible recombinases in 
            mammalian cells and mice.
  JOURNAL   Nat Commun. 2019 Oct 24;10(1):4845. doi: 10.1038/s41467-019-12800-7.
  PUBMED    31649244
REFERENCE   2  (bases 1 to 5628)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-10-24"; volume: "10(1):4845. doi"; pages: " 
            10.1038/s41467-019-12800-7"
COMMENT     Sequence Label: pBW1358_pCAG-PV1-FKBP-L1-PhiC31-397C-NLS-BGHpA
FEATURES             Location/Qualifiers
     source          1..5628
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     466..488
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     550..569
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             686..1006
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             1676..1696
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    1706..1930
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(2170..2189)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    2235..2290
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2289..2308)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(2651..2667)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2651..2667)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2664..2686)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2675..2691
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2699..2705,2706..2723,2724..2729))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2744..2765
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      2850..2984
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    3004..3138
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3041..3060)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3095..3114
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3206..3223)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3377..3965)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3457..3476)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4136..4927,4928..4996))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4759..4778
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4997..5101)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        5132..5511
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tgcacccccc tccccgagtt gctgagcacg gcccggcttc gggtgcgggg ctccgtacgg
       61 ggcgtggcgc ggggctcgcc gtgccgggcg gggggtggcg gcaggtgggg gtgccgggcg
      121 gggcggggcc gcctcgggcc ggggagggct cgggggaggg gcgcggcggc ccccggagcg
      181 ccggcggctg tcgaggcgcg gcgagccgca gccattgcct tttatggtaa tcgtgcgaga
      241 gggcgcaggg acttcctttg tcccaaatct gtgcggagcc gaaatctggg aggcgccgcc
      301 gcaccccctc tagcgggcgc ggggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg
      361 gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct ccctctccag cctcggggct
      421 gtccgcgggg ggacggctgc cttcgggggg gacggggcag ggcggggttc ggcttctggc
      481 gtgtgaccgg cggctctaga gcctctgcta accatgttca tgccttcttc tttttcctac
      541 agctcctggg caacgtgctg gttattgtgc tgtctcatca ttttggcaaa gaattgatta
      601 attcgagcga aggcgcgccc attactcgca tccattctca ggctgtctcg tctcgtctcg
      661 cacgcgtgcc gccaccatgt ctagaggagt gcaggtggaa accatctccc caggagacgg
      721 gcgcaccttc cccaagcgcg gccagacctg cgtggtgcac tacaccggga tgcttgaaga
      781 tggaaagaaa tttgattcct cccgggacag aaacaagccc tttaagttta tgctaggcaa
      841 gcaggaggtg atccgaggct gggaagaagg ggttgcccag atgagtgtgg gtcagagagc
      901 caaactgact atatctccag attatgccta tggtgccact gggcacccag gcatcatccc
      961 accacatgcc actctcgtct tcgatgtgga gcttctaaaa ctggaatccg gagggtctgg
     1021 ctccggatca agtggtggca gcggtaccgc ccctggccag cacgagggca cctgtaatgt
     1081 gagcatggcc gccctggaca agttcgtggc cgagcggatc ttcaacaaga tccggcacgc
     1141 cgagggcgac gaggagaccc tggccctgct gtgggaggcc gccagaagat tcggcaagct
     1201 gaccgaggcc cccgagaaga gcggcgagag ggccaacctg gtggccgaga gagccgacgc
     1261 cctgaacgcc ctggaggagc tgtacgagga cagagccgcc ggagcctatg acggccctgt
     1321 gggcaggaag cacttcagaa agcagcaggc cgccctgacc ctgagacagc agggcgccga
     1381 ggaaagactg gccgagctgg aggccgccga ggcccctaag ctgcccctgg atcagtggtt
     1441 ccccgaggat gccgacgccg accccaccgg ccccaagtcc tggtggggca gagccagcgt
     1501 ggacgacaag agggtgttcg tgggcctgtt cgtggataag atcgtggtga ccaagagcac
     1561 caccggcagg ggccagggca cccccatcga gaagagagcc agcatcacct gggccaagcc
     1621 tcccaccgac gacgacgagg atgacgccca ggacggcacc gaggacgtgg ccgcccccaa
     1681 gaaaaagcgg aaggtgtgag aattcctgtg ccttctagtt gccagccatc tgttgtttgc
     1741 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa
     1801 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg
     1861 gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg
     1921 ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg
     1981 ccctgtagcg tgtaccgctg ggagttcgta gacggaaaca aacgcagaat ccaagcgcgg
     2041 ccgcccagga tacatagatt accacaactc cgagcccttc caccgctagc gcactcctca
     2101 ggtgcaggct gcctatcaga aggtggtggc tggtgtggcc aatgccctgg ctcacaaata
     2161 ccactgagat ctttttccct ctgccaaaaa ttatggggac atcatgaagc cccttgagca
     2221 tctgacttct ggctaataaa ggaaatttat tttcattgca atagtgtgtt ggaatttttt
     2281 gtgtctctca ctcggaagga catatgggag ggcaaatcat ttaaaacatc agaatgagta
     2341 tttggtttag agtttggcaa catatgccca tatgctggct gccatgaaca aaggttggct
     2401 ataaagaggt catcagtata tgaaacagcc ccctgctgtc cattccttat tccatagaaa
     2461 agccttgact tgaggttaga ttttttttat attttgtttt gtgttatttt tttctttaac
     2521 atccctaaaa ttttccttac atgttttact agccagattt ttcctcctct cctgactact
     2581 cccagtcata gctgtccctc ttctcttatg gagatccctc gacctgcagc ccaagcttgg
     2641 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     2701 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     2761 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagcgga
     2821 tccgcatctc aattagtcag caaccatagt cccgccccta actccgccca gttccgccca
     2881 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
     2941 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
     3001 gctaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     3061 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     3121 tcttatcatg tctggatccg ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     3181 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     3241 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     3301 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     3361 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     3421 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     3481 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     3541 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     3601 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     3661 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     3721 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     3781 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     3841 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     3901 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     3961 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     4021 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     4081 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     4141 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     4201 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     4261 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     4321 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     4381 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     4441 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     4501 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     4561 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     4621 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     4681 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     4741 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     4801 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     4861 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     4921 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     4981 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     5041 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     5101 gcacatttcc ccgaaaagtg ccacctgggt cgacattgat tattgactag ttattaatag
     5161 taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt
     5221 acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg
     5281 acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat
     5341 ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct
     5401 attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg
     5461 gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat ggtcgaggtg
     5521 agccccacgt tctgcttcac tctccccatc tcccccccct ccccaccccc aattttgtat
     5581 ttatttattt tttaattatt ttgtgcagcg atgggggcgg gggggggg
//
