LOCUS       sequence-21739-e         914 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 914)
  AUTHORS   Sablina AA, Chen W, Arroyo JD, Corral L, Hector M, Bulmer SE, 
            DeCaprio JA, Hahn WC
  TITLE     The tumor suppressor PP2A Abeta regulates the RalA GTPase.
  JOURNAL   Cell. 2007 Jun 1. 129(5):969-82.
  PUBMED    17540176
REFERENCE   2  (bases 1 to 914)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2007 
            Jun 1. 129(5):969-82."
COMMENT     Sequence Label: pMKO.1-Puro-shPP2A-Aalpha
FEATURES             Location/Qualifiers
     source          1..914
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        54..302
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     54..74
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     225..244
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(387..407)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        392..721
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      572..707
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(731..750)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 actccttctc taggcgccgg aattgaagat ctgtcgacaa ggtcgggcag gaagagggcc
       61 tatttcccat gattccttca tatttgcata tacgatacaa ggctgttaga gagataatta
      121 gaattaattt gactgtaaac acaaagatat tagtacaaaa tacgtgacgt agaaagtaat
      181 aatttcttgg gtagtttgca gttttaaaat tatgttttaa aatggactat catatgctta
      241 ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa ggacgaaaca
      301 ccgggacaac agcaccttgc agagtctcga gactctgcaa ggtgctgttg tctttttgaa
      361 ttcgccagca cagtggtcga ccctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
      421 aggcttccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
      481 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
      541 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
      601 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
      661 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
      721 aaagcttacc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc
      781 cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt
      841 cgatcggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc
      901 gggctcgaca tcgg
//
