LOCUS       sequence-217428-        6901 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses worm truncated KVS1.2 C113S in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6901)
  AUTHORS   Cai SQ, Sesti F
  TITLE     Oxidation of a potassium channel causes progressive sensory function
            loss during aging.
  JOURNAL   Nat Neurosci. 2009 May;12(5):611-7. doi: 10.1038/nn.2291. Epub 2009 
            Mar 29.
  PUBMED    19330004
REFERENCE   2  (bases 1 to 6901)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nn.2291"; 
            journalName: "Nat Neurosci"; date: "2009-05"; volume: "12"; issue: 
            "5"; pages: "611-7"
COMMENT     Sequence Label: DeltaNIRD C95S KVS-1
FEATURES             Location/Qualifiers
     source          1..6901
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..212
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     163..183
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     209..233
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          373..505
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     488..507
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        550..568
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1996..2022
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    2088..2209
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2099..2118)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2153..2172
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      2395..2850
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2482..2501)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2692..2713
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2967..3324
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        2967..3158
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(2990..3010)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3175..3310
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3375..4169
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3429..3448)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4039..4058
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    4233..4281
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(4322..4341)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4441..4463
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4501..4519)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4587..4691
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4692..4760,4761..5552)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4910..4929)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5723..6311
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6212..6231
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        6522..6901
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta caccaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctgcgatcgc ccgccccgtt gacgcaaatg ggcggtaggc
      181 gtgtacggtg ggaggtctat ataagcagag ctcgtttagt gaaccgtcag atcactagaa
      241 gctttattgc ggtagtttat cacagttaaa ttgctaacgc agtcagtgct tctgacacaa
      301 cagtctcgaa cttaagctgc agtgactctc ttaaggtagc cttgcagaag ttggtcgtga
      361 ggcactgggc aggtaagtat caaggttaca agacaggttt aaggagacca atagaaactg
      421 ggcttgtcga gacagagaag actcttgcgt ttctgatagg cacctattgg tcttactgac
      481 atccactttg cctttctctc cacaggtgtc cactcccagt tcaattacag ctcttaaggc
      541 tagagtactt aatacgactc actataggct agcctcgaga tggtgattcc cagcaccagg
      601 aaaggacatg ctgcatctcg tgacaatttt ggcaactcta accatcatgg tgacgagatt
      661 gtgcaccgac tcaacaatgg gagacgttca gagatagatg aagtaaagcc cgaccttgtg
      721 tcaggggccg gcgacactgt gatcaagttg aatgttggag ggacatcatt cagaataagg
      781 atatccagca tctttgcaag atcggcggac gagaaactgt ccgcgtttgc acaattaaac
      841 catgagcaga gacttcaagc atctgacgcg tttcttatgc cacaagagga gtactacttg
      901 gaaaggtcgg ctgccctttt tgattcgatc ttcaaatact acatatctgg ccaactacac
      961 agaccgcttg atgtatgccc cacagaattt gccaccgaac tcgcctactg gaagataaac
     1021 gacgggctca cgtcaaactg ctgttggagg ggatacaata tgagtccttc actagaagaa
     1081 ctggtggccc cgaaagagaa aaaaatagag ctgcacgacc gttcaatgcg ctcacggatt
     1141 catcaattct gcgaagggga cggctcactt ttctcgactt tgtttacttt tggcagcatc
     1201 gcttttgttc ttattagtgt tattggactg gttttgggat ccattcacga atttcaggta
     1261 cctatctata aaaaaggagg aaaatcagag aggatcagca actggagtat cgtggaaaac
     1321 gaaaacatta tatgggagcc acatccgatt tttgggtatg tggagacagt gtgtatcatc
     1381 tggtttactg tagaattcat cgtacggtta gcagttacac caaatagatt ccaatttttg
     1441 gtgggaatca tgaatattgt cgatatgatc gctattattc cattctattt ggaactcggg
     1501 ttggcgttgt ttggaattga tgtcgctagt ctgagcgaca ttaaaggagc actattagtt
     1561 gttagagtgc tccgtgtgcc tcgtgttgtg agaattttga aacttggaag atactcatct
     1621 ggtatgcgaa cattcgcttt gacactgaga agttccgctc gccagctggg aatgatggga
     1681 atggtcctgt caaccggtgt cgtattcttc tcaacacttt tatacttcgt cgagaaagac
     1741 gaaaaagaca cgccgttcac ttcgattcca gctgcttttt ggtgggcaat cgtcacgatg
     1801 acaactgttg gatatggaga ttatgttcct gtgacagttc ctggaaaact gattgcaagt
     1861 ggagcaatta tcagtggagt attggtactt gctcttccta tcactatcat cgttgacaac
     1921 tttatgaaag tttctggaaa tgtgaatgcc aacatcttct catctgcaaa ccaacgacct
     1981 attgatgtgg cagaataccc atacgacgtc ccagactacg cttaatctag agtcgacccg
     2041 ggcggccgct tccctttagt gagggttaat gcttcgagca gacatgataa gatacattga
     2101 tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg
     2161 tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa
     2221 ttgcattcat tttatgtttc aggttcaggg ggagatgtgg gaggtttttt aaagcaagta
     2281 aaacctctac aaatgtggta aaatccgata aggatcgatc cgggctggcg taatagcgaa
     2341 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggacgcgc
     2401 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     2461 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     2521 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     2581 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
     2641 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     2701 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
     2761 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     2821 attttaacaa aatattaacg cttacaattt cctgatgcgg tattttctcc ttacgcatct
     2881 gtgcggtatt tcacaccgca tacgcggatc tgcgcagcac catggcctga aataacctct
     2941 gaaagaggaa cttggttagg taccttctga ggcggaaaga accagctgtg gaatgtgtgt
     3001 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     3061 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     3121 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     3181 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     3241 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     3301 tttggaggcc taggcttttg caaaaagctt gattcttctg acacaacagt ctcgaactta
     3361 aggctagagc caccatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg
     3421 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg
     3481 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg
     3541 ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc
     3601 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg
     3661 aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca
     3721 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc
     3781 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg
     3841 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg
     3901 cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata
     3961 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg
     4021 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat
     4081 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct
     4141 tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca
     4201 agcgacgccc aacctgccat cacgatggcc gcaataaaat atctttattt tcattacatc
     4261 tgtgtgttgg ttttttgtgt gaatcgatag cgataaggat ccgcgtatgg tgcactctca
     4321 gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg
     4381 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
     4441 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg
     4501 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
     4561 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     4621 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     4681 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     4741 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     4801 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     4861 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     4921 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     4981 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     5041 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     5101 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     5161 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     5221 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     5281 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     5341 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     5401 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     5461 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     5521 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     5581 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     5641 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     5701 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     5761 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     5821 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     5881 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     5941 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     6001 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     6061 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     6121 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     6181 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     6241 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     6301 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     6361 ttgctcacat ggctcgacag atcttcaata ttggccatta gccatattat tcattggtta
     6421 tatagcataa atcaatattg gctattggcc attgcatacg ttgtatctat atcataatat
     6481 gtacatttat attggctcat gtccaatatg accgccatgt tggcattgat tattgactag
     6541 ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt
     6601 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
     6661 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
     6721 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
     6781 tccgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
     6841 gaccttacgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
     6901 g
//
