LOCUS       sequence-217430-        8472 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses cAICDA-BE3 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8472)
  AUTHORS   Wang X, Li J, Wang Y, Yang B, Wei J, Wu J, Wang R, Huang X, Chen J, 
            Yang L
  TITLE     Efficient base editing in methylated regions with a human 
            APOBEC3A-Cas9 fusion.
  JOURNAL   Nat Biotechnol. 2018 Aug 20. pii: nbt.4198. doi: 10.1038/nbt.4198.
  PUBMED    30125268
REFERENCE   2  (bases 1 to 8472)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2018 Aug 20. pii: nbt.4198. doi: 10.1038/nbt.4198."
COMMENT     Sequence Label: pCMV-cAICDA-BE3
FEATURES             Location/Qualifiers
     source          1..8472
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..29)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        201..580
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        581..784
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     735..755
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     826..845
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        826..844
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(1533..4931,4932..4934,4935..5531,5532,5533..5535,
                     5536..5537,5538..5540,5541..5633)
                     /codon_start=1
                     /product="Streptococcus pyogenes Cas9 endonuclease with the
                     D1135V/R1335Q/T1337R mutations"
                     /label=Cas9 VQR
                     /note="has an altered recognition specificity for the 
                     protospacer adjacent motif (PAM) (Kleinstiver et al., 
                     2015)"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             join(1533..1556,1557..1559,1560..5633)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5646..5894
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             5907..5927
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5936..5953
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5976..5993)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5982..6206
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6277..6293)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6277..6293)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6290..6312)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6301..6317
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6325..6331,6332..6349,6350..6355))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6370..6391
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6508..6525)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6679..7267)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6759..6778)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7438..8229,8230..8298))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8061..8080
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8299..8403)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gactctcagt acaatctgct ctgatgccgc atagttaagc cagtatctgc tccctgcttg
       61 tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt
      121 gaccgacaat tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt
      181 acgggccaga tatacgcgtt gacattgatt attgactagt tattaatagt aatcaattac
      241 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg
      301 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc
      361 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac
      421 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa
      481 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac
      541 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta
      601 catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga
      661 cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa
      721 ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag
      781 agctggttta gtgaaccgtc agatccgcta gagatccgcg gccgctaata cgactcacta
      841 tagggagagc cgccaccatg agcaagctgg acagtgtgct gctgactcag aggaagttta
      901 tttaccacta taagaatgtg cgctgggctc gtgggaggaa cgagacctac ctctgttttg
      961 tggtcaagaa acgcaacagt cccgactcgc tctccttcga cttcggacac ctgcgcaatc
     1021 gttctggctg ccatgtggag cttctcttcc tgagctatct tggggtactg tgcccaggtt
     1081 tcttgggttc cggtgtggat ggtgtcaggg tggcttatgc catcacctgg ttctgttcct
     1141 ggtcaccctg ttcaaactgt gcccatcgcc tttctcgctt catgtctcag atgcccaacc
     1201 tgcggctgcg catcttcgtc tcgcgcctct acttctgtga cgaggaggac agtcaagaga
     1261 gagagggact ccgttgcttg cagagggcag gtgtgcaagt gacagtcatg acctataaag
     1321 attttttcta ctgttggcaa acctttgtgg ctcaaaatca gaaggctttc aaggcttggg
     1381 acgaccttca ccagaactct atccgactgt ctcggaaact acagcgaatc ctgcagccta
     1441 gtgagtctga agacctgagg gatggcttcg ctctgctggg ccttagcggc agcgagactc
     1501 ccgggacctc agagtccgcc acacccgaaa gtgataaaaa gtattctatt ggtttagcca
     1561 tcggcactaa ttccgttgga tgggctgtca taaccgatga atacaaagta ccttcaaaga
     1621 aatttaaggt gttggggaac acagaccgtc attcgattaa aaagaatctt atcggtgccc
     1681 tcctattcga tagtggcgaa acggcagagg cgactcgcct gaaacgaacc gctcggagaa
     1741 ggtatacacg tcgcaagaac cgaatatgtt acttacaaga aatttttagc aatgagatgg
     1801 ccaaagttga cgattctttc tttcaccgtt tggaagagtc cttccttgtc gaagaggaca
     1861 agaaacatga acggcacccc atctttggaa acatagtaga tgaggtggca tatcatgaaa
     1921 agtacccaac gatttatcac ctcagaaaaa agctagttga ctcaactgat aaagcggacc
     1981 tgaggttaat ctacttggct cttgcccata tgataaagtt ccgtgggcac tttctcattg
     2041 agggtgatct aaatccggac aactcggatg tcgacaaact gttcatccag ttagtacaaa
     2101 cctataatca gttgtttgaa gagaacccta taaatgcaag tggcgtggat gcgaaggcta
     2161 ttcttagcgc ccgcctctct aaatcccgac ggctagaaaa cctgatcgca caattacccg
     2221 gagagaagaa aaatgggttg ttcggtaacc ttatagcgct ctcactaggc ctgacaccaa
     2281 attttaagtc gaacttcgac ttagctgaag atgccaaatt gcagcttagt aaggacacgt
     2341 acgatgacga tctcgacaat ctactggcac aaattggaga tcagtatgcg gacttatttt
     2401 tggctgccaa aaaccttagc gatgcaatcc tcctatctga catactgaga gttaatactg
     2461 agattaccaa ggcgccgtta tccgcttcaa tgatcaaaag gtacgatgaa catcaccaag
     2521 acttgacact tctcaaggcc ctagtccgtc agcaactgcc tgagaaatat aaggaaatat
     2581 tctttgatca gtcgaaaaac gggtacgcag gttatattga cggcggagcg agtcaagagg
     2641 aattctacaa gtttatcaaa cccatattag agaagatgga tgggacggaa gagttgcttg
     2701 taaaactcaa tcgcgaagat ctactgcgaa agcagcggac tttcgacaac ggtagcattc
     2761 cacatcaaat ccacttaggc gaattgcatg ctatacttag aaggcaggag gatttttatc
     2821 cgttcctcaa agacaatcgt gaaaagattg agaaaatcct aacctttcgc ataccttact
     2881 atgtgggacc cctggcccga gggaactctc ggttcgcatg gatgacaaga aagtccgaag
     2941 aaacgattac tccatggaat tttgaggaag ttgtcgataa aggtgcgtca gctcaatcgt
     3001 tcatcgagag gatgaccaac tttgacaaga atttaccgaa cgaaaaagta ttgcctaagc
     3061 acagtttact ttacgagtat ttcacagtgt acaatgaact cacgaaagtt aagtatgtca
     3121 ctgagggcat gcgtaaaccc gcctttctaa gcggagaaca gaagaaagca atagtagatc
     3181 tgttattcaa gaccaaccgc aaagtgacag ttaagcaatt gaaagaggac tactttaaga
     3241 aaattgaatg cttcgattct gtcgagatct ccggggtaga agatcgattt aatgcgtcac
     3301 ttggtacgta tcatgacctc ctaaagataa ttaaagataa ggacttcctg gataacgaag
     3361 agaatgaaga tatcttagaa gatatagtgt tgactcttac cctctttgaa gatcgggaaa
     3421 tgattgagga aagactaaaa acatacgctc acctgttcga cgataaggtt atgaaacagt
     3481 taaagaggcg tcgctatacg ggctggggac gattgtcgcg gaaacttatc aacgggataa
     3541 gagacaagca aagtggtaaa actattctcg attttctaaa gagcgacggc ttcgccaata
     3601 ggaactttat gcagctgatc catgatgact ctttaacctt caaagaggat atacaaaagg
     3661 cacaggtttc cggacaaggg gactcattgc acgaacatat tgcgaatctt gctggttcgc
     3721 cagccatcaa aaagggcata ctccagacag tcaaagtagt ggatgagcta gttaaggtca
     3781 tgggacgtca caaaccggaa aacattgtaa tcgagatggc acgcgaaaat caaacgactc
     3841 agaaggggca aaaaaacagt cgagagcgga tgaagagaat agaagagggt attaaagaac
     3901 tgggcagcca gatcttaaag gagcatcctg tggaaaatac ccaattgcag aacgagaaac
     3961 tttacctcta ttacctacaa aatggaaggg acatgtatgt tgatcaggaa ctggacataa
     4021 accgtttatc tgattacgac gtcgatcaca ttgtacccca atcctttttg aaggacgatt
     4081 caatcgacaa taaagtgctt acacgctcgg ataagaaccg agggaaaagt gacaatgttc
     4141 caagcgagga agtcgtaaag aaaatgaaga actattggcg gcagctccta aatgcgaaac
     4201 tgataacgca aagaaagttc gataacttaa ctaaagctga gaggggtggc ttgtctgaac
     4261 ttgacaaggc cggatttatt aaacgtcagc tcgtggaaac ccgccaaatc acaaagcatg
     4321 ttgcacagat actagattcc cgaatgaata cgaaatacga cgagaacgat aagctgattc
     4381 gggaagtcaa agtaatcact ttaaagtcaa aattggtgtc ggacttcaga aaggattttc
     4441 aattctataa agttagggag ataaataact accaccatgc gcacgacgct tatcttaatg
     4501 ccgtcgtagg gaccgcactc attaagaaat acccgaagct agaaagtgag tttgtgtatg
     4561 gtgattacaa agtttatgac gtccgtaaga tgatcgcgaa aagcgaacag gagataggca
     4621 aggctacagc caaatacttc ttttattcta acattatgaa tttctttaag acggaaatca
     4681 ctctggcaaa cggagagata cgcaaacgac ctttaattga aaccaatggg gagacaggtg
     4741 aaatcgtatg ggataagggc cgggacttcg cgacggtgag aaaagttttg tccatgcccc
     4801 aagtcaacat agtaaagaaa actgaggtgc agaccggagg gttttcaaag gaatcgattc
     4861 ttccaaaaag gaatagtgat aagctcatcg ctcgtaaaaa ggactgggac ccgaaaaagt
     4921 acggtggctt cgatagccct acagttgcct attctgtcct agtagtggca aaagttgaga
     4981 agggaaaatc caagaaactg aagtcagtca aagaattatt ggggataacg attatggagc
     5041 gctcgtcttt tgaaaagaac cccatcgact tccttgaggc gaaaggttac aaggaagtaa
     5101 aaaaggatct cataattaaa ctaccaaagt atagtctgtt tgagttagaa aatggccgaa
     5161 aacggatgtt ggctagcgcc ggagagcttc aaaaggggaa cgaactcgca ctaccgtcta
     5221 aatacgtgaa tttcctgtat ttagcgtccc attacgagaa gttgaaaggt tcacctgaag
     5281 ataacgaaca gaagcaactt tttgttgagc agcacaaaca ttatctcgac gaaatcatag
     5341 agcaaatttc ggaattcagt aagagagtca tcctagctga tgccaatctg gacaaagtat
     5401 taagcgcata caacaagcac agggataaac ccatacgtga gcaggcggaa aatattatcc
     5461 atttgtttac tcttaccaac ctcggcgctc cagccgcatt caagtatttt gacacaacga
     5521 tagatcgcaa acgatacact tctaccaagg aggtgctaga cgcgacactg attcaccaat
     5581 ccatcacggg attatatgaa actcggatag atttgtcaca gcttgggggt gactctggtg
     5641 gttctactaa tctgtcagat attattgaaa aggagaccgg taagcaactg gttatccagg
     5701 aatccatcct catgctccca gaggaggtgg aagaagtcat tgggaacaag ccggaaagcg
     5761 atatactcgt gcacaccgcc tacgacgaga gcaccgacga gaatgtcatg cttctgacta
     5821 gcgacgcccc tgaatacaag ccttgggctc tggtcataca ggatagcaac ggtgagaaca
     5881 agattaagat gctctctggt ggttctccca agaagaagag gaaagtctaa ccggtcatca
     5941 tcaccatcac cattgagttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca
     6001 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
     6061 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
     6121 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca
     6181 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctcgat
     6241 accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     6301 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagccta
     6361 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     6421 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     6481 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     6541 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     6601 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     6661 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     6721 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     6781 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     6841 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     6901 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     6961 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     7021 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     7081 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     7141 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     7201 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     7261 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     7321 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     7381 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     7441 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     7501 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     7561 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     7621 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     7681 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     7741 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     7801 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     7861 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     7921 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     7981 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     8041 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     8101 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     8161 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     8221 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     8281 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     8341 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     8401 gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc gatctcccga
     8461 tcccctaggg tc
//
