LOCUS       sequence-217436-       11611 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Beta-estradiol inducible 3X-FLAG Ty3 Integrase; Z4 promoter-3XFLAG 
            Ty3 IN-CYC1 terminator; artificial transcription factor under ACT1 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11611)
  AUTHORS   Patterson K, Shavarebi F, Magnan C, Chang I, Qi X, Baldi P, 
            Bilanchone V, Sandmeyer SB
  TITLE     Local features determine Ty3 targeting frequency at RNA polymerase 
            III transcription start sites.
  JOURNAL   Genome Res. 2019 Aug;29(8):1298-1309. doi: 10.1101/gr.240861.118. 
            Epub 2019 Jun 27.
  PUBMED    31249062
REFERENCE   2  (bases 1 to 11611)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gr.240861.118"; journalName: "Genome Res"; date: "2019-08";
            volume: "29"; issue: "8"; pages: "1298-1309"
COMMENT     Sequence Label: pKP4010
FEATURES             Location/Qualifiers
     source          1..11611
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      133..382
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(149..167)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     CDS             complement(2025..2048)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(2052..2075)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(2079..2102)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(2129..2152)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     promoter        2876..3219
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             3220..4029
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3287..3306)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     4655..4671
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     4656..4672
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      6191..6378
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(6246..6270)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     misc_feature    7078..8240
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN4 centromere fused to the 
                     autonomously replicating sequence ARS1/ARS416"
     rep_origin      7403..8240
                     /label=ARS1
                     /note="S. cerevisiae autonomously replicating sequence 
                     ARS1/ARS416"
     CDS             complement(8575..9378)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(9379..9594)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(9619..9637)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9705..9809
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9810..9878,9879..10670)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10028..10047)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10841..11429
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11330..11349
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11583..11600
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gttaacttaa ttaagtttaa actccggaca agatggcctt tggtgggttg aagaaggaaa
       61 aagacagaaa cgacttaatt acctacttga aaaaagcctg tgagtaaaca ggcccctttt
      121 cctttgtcga tatcatgtaa ttagttatgt cacgcttaca ttcacgccct cctcccacat
      181 ccgctctaac cgaaaaggaa ggagttagac aacctgaagt ctaggtccct atttattttt
      241 tttaatagtt atgttagtat taagaacgtt atttatattt caaatttttc ttttttttct
      301 gtacaaacgc gtgtacgcat gtaacattat actgaaaacc ttgcttgaga aggttttggg
      361 acgctcgaag gctttaattt gcaaagtcag ggtgacgtat tgtcatactg acatatctca
      421 ttttgagata caacatcttc ctctctctca gggttgtctt gtgtttcgta gagttgtcta
      481 aagttggcta atattgatct tcgtgttctt tcgggaattt ggcaaaattc agcttctgag
      541 tattctactg aaagtgttgg gtctacatct tgcatgtgac ataagtaagt tttgtgtgta
      601 gtatctattc ctatgagtgc agtaacttcg tgtgctctct taattctttc agtggagctg
      661 attggtttat tctttgggta cgcgtctgga cggtatacaa actttttcag gaattgtaca
      721 ttaataactc tgtgcttttt cttgtgagag tttaaatcta gttcgtaggc gttatcgttt
      781 attttcttga caactcgaaa tggtccgacg tatatttgtt gtactttcat ataagcacct
      841 ttcttgaagt atgcatctct atgcactaat acgtgatctc ctatgtttaa caataagggt
      901 ttacgtcttt gattgttatt agtttccatt tcgatttgag cgtgttctag ctgttccttc
      961 gtttggatgg taagggcttt gaggtgtttg gcaagttcta cggcagtaaa acttcttgcg
     1021 ttgacttcgt catctgactt aatagcaggg gtattcggta aatatcctaa atcaatttca
     1081 aatggtgatt ttccaagtgt tctagtaggt gtagaattgt aaacaaattc gatttgtggt
     1141 aaatatacat gccaattctg aatattggtt gaagcatagg ctcttagtaa cctgtttaat
     1201 gtctgtatcg ttcgttcgga ttgtccatct gtttgggggt ggttcgcgga agacattgtc
     1261 gattttattc ctagtctttt cgtgagttct tgatatttgt cggcggtcat acggacatct
     1321 ctatcactgg ttattgtcct gggaaaacca tgatatgaaa aaatgtatcg aaagagtaga
     1381 tctattagtt gtgttgcgtc taaggttttc cttgtagcta tgaagtgagc gcgtttcgaa
     1441 aaacgatcaa ctacgacgag gatcatattc aagttatttg atgtcggggg taatcctgtc
     1501 acaaaatcca ttgatatatc aagccatctt ccttctgcta tagggagtgg ttgtaatagt
     1561 ccatgtaagc gtggtcggtg tgattttatt agttgacatt gtacgcaggt cctgatgtat
     1621 tgtatgatcg aatgttgtaa ttttggccaa tagtaaattg ggctgatttt cgcaagggtc
     1681 actgttacac caaaatgtcc tccaaataag gtatggtcat gatatagtct cataactgcg
     1741 ttctgttgtt ttattggtac tactagtcgg tcttggtaat agatcatttc gtcttctagg
     1801 gaataattct ttcggaaggt ctctgatagt tcgagtttct tctggtaact acggaaggct
     1861 gacatatctt caggtgtgac gttgtgttgt gtcaattctt tcatatgaat taagacagca
     1921 ctacataatg ggtctgattt gtagtaagat ttccagcttt ctgtgtcgat aggtcgggat
     1981 gtttcggggg ttatagtacc gcctccaccc ccgcctcccc ctcccttatc gtcgtcatcc
     2041 ttgtaatccc ccttatcgtc gtcatccttg taatccccct tatcgtcgtc atccttgtaa
     2101 tcacccatgt atatagtttt ttctccttga cgttaaagta tagaggtata ttaacaattt
     2161 tttgttgata cttttatgac atttgaataa gaagtaatac aaactgaaaa tgttgaaagt
     2221 attagttaaa gtggttatgc agcttttcca tttatatatc tgttaataga tcaaaaatca
     2281 tcgcttcgct gattaattac cccagaaata aggctaaaaa actaatcgca ttatcatcct
     2341 atggttgtta atttgattcg ttaatttgaa ggtttgtggg gccaggttac tgccaatttt
     2401 tcctcttcat aaccataaaa gctagtattg tagaatcttt attgttcgga gcagtgcggc
     2461 gcgaggcaca tctgcgtttc aggaacgcga ccggtgaaga cgaggacgca cggtctagac
     2521 tcctccgccg cactcctccg ccgctcctcc tccgccgcac tcctccgccg ctcctcctcc
     2581 gccgcactcc tccgccgcgg ccgcacttca atatagcaat gagcagttaa gcgtattact
     2641 gaaagttcca aagagaaggt ttttttaggc taagataatg gggctcttta catttccaca
     2701 acatataagt aagattagat atggatatgt atatggatat gtatatggtg gtaatgccat
     2761 gtaatatgat tattaaactt ctttgcgtcc atccaaaaaa aaagtaagaa tttttgaaaa
     2821 ttcaatataa agatctgttt agcttgcctc gtccccgccg ggtcacccgg ccagcgacat
     2881 ggaggcccag aataccctcc ttgacagtct tgacgtgcgc agctcagggg catgatgtga
     2941 ctgtcgcccg tacatttagc ccatacatcc ccatgtataa tcatttgcat ccatacattt
     3001 tgatggccgc acggcgcgaa gcaaaaatta cggctcctcg ctgcagacct gcgagcaggg
     3061 aaacgctccc ctcacagacg cgttgaattg tccccacgcc gcgcccctgt agagaaatat
     3121 aaaaggttag gatttgccac tgaggttctt ctttcatata cttcctttta aaatcttgct
     3181 aggatacagt tctcacatca catccgaaca taaacaacca tgggtaagga aaagactcac
     3241 gtttcgaggc cgcgattaaa ttccaacatg gatgctgatt tatatgggta taaatgggct
     3301 cgcgataatg tcgggcaatc aggtgcgaca atctatcgat tgtatgggaa gcccgatgcg
     3361 ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca atgatgttac agatgagatg
     3421 gtcagactaa actggctgac ggaatttatg cctcttccga ccatcaagca ttttatccgt
     3481 actcctgatg atgcatggtt actcaccact gcgatccccg gcaaaacagc attccaggta
     3541 ttagaagaat atcctgattc aggtgaaaat attgttgatg cgctggcagt gttcctgcgc
     3601 cggttgcatt cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc
     3661 gctcaggcgc aatcacgaat gaataacggt ttggttgatg cgagtgattt tgatgacgag
     3721 cgtaatggct ggcctgttga acaagtctgg aaagaaatgc ataagctttt gccattctca
     3781 ccggattcag tcgtcactca tggtgatttc tcacttgata accttatttt tgacgagggg
     3841 aaattaatag gttgtattga tgttggacga gtcggaatcg cagaccgata ccaggatctt
     3901 gccatcctat ggaactgcct cggtgagttt tctccttcat tacagaaacg gctttttcaa
     3961 aaatatggta ttgataatcc tgatatgaat aaattgcagt ttcatttgat gctcgatgag
     4021 tttttctaat cagtactgac aataaaaaga ttcttgtttt caagaacttg tcatttgtat
     4081 agttttttta tattgtagtt gttctatttt aatcaaatgt tagcgtgatt tatatttttt
     4141 ttcgcctcga catcatctgc ccagatgcga agttaagtgc gggccggccc gtacgctcac
     4201 cctaacatat tttccaatta acctcaatat ttctctgtca cccggcctct attttccatt
     4261 ttcttcttta cccgccacgc gtttttttct ttcaaatttt tttcttcctt cttctttttc
     4321 ttccacgtcc tcttgcataa ataaataaac cgttttgaaa ccaaactcgc ctctctctct
     4381 cctttttgaa atatttttgg gtttgtttga tcctttcctt cccaatctct cttgtttaat
     4441 atatattcat ttatatcacg ctctcttttt atcttccttt ttttcctctc tcttgtattc
     4501 ttccttcccc tttctactca aaccaagaag aaaaagaaaa ggtcaatctt tgttaaagaa
     4561 taggatcttc tactacatca gcttttagat ttttcacgct tactgctttt ttcttcccaa
     4621 gatcgaaaat ttactgaatt aacagggccc cccctcgagg tcgacggtat cgataagctt
     4681 gaagcaagcc tcctgaaaga tgggtacccg cccatatgct tgccctgtcg agtcctgcga
     4741 tcgccgcttt tctcgccacg ccaatcttac ccgccatatc cgcatccata ccggtcagaa
     4801 gcccttccag tgtcgaatct gcatgcgtaa cttcagtcgt aatgcgaacc ttgtgcgcca
     4861 catccgcacc cacacaggat cccaaaagcc gttccaatgt cggatctgta tgcggaactt
     4921 tagtcgaaag gccgacctga ggcgtcacat tcgcacgcac accggcgaga agccttttgc
     4981 ctgtgacatt tgtgggagga agtttgccag gaagggcgac ctcaagaggc ataccaaaat
     5041 ccatacaggt ggcggaggca cacctgcagc tgcgtcgact ctagaggatc catctgctgg
     5101 agacatgaga gctgccaacc tttggccaag cccgctcatg atcaaacgct ctaagaagaa
     5161 cagcctggcc ttgtccctga cggccgacca gatggtcagt gccttgttgg atgctgagcc
     5221 ccccatactc tattccgagt atgatcctac cagacccttc agtgaagctt cgatgatggg
     5281 cttactgacc aacctggcag acagggagct ggttcacatg atcaactggg cgaagagggt
     5341 gccaggcttt gtggatttga ccctccatga tcaggtccac cttctagaat gtgcctggct
     5401 agagatcctg atgattggtc tcgtctggcg ctccatggag cacccagtga agctactgtt
     5461 tgctcctaac ttgctcttgg acaggaacca gggaaaatgt gtagagggca tggtggagat
     5521 cttcgacatg ctgctggcta catcatctcg gttccgcatg atgaatctgc agggagagga
     5581 gtttgtgtgc ctcaaatcta ttattttgct taattctgga gtgtacacat ttctgtccag
     5641 caccctgaag tctctggaag agaaggacca tatccaccga gtcctggaca agatcacaga
     5701 cactttgatc cacctgatgg ccaaggcagg cctgaccctg cagcagcagc accagcggct
     5761 ggcccagctc ctcctcatcc tctcccacat caggcacatg agtaacaaag gcatggagca
     5821 tctgtacagc atgaagtgca agaacgtggt gcccctctat gacctgctgc tggagatgct
     5881 ggacgcccac cgcctacatg cgcccactag ccgtggaggg gcatccgtgg aggagacgga
     5941 ccaaagccac ttggccactg cgggctctac ttcatcggag ctccacttag acggcgagga
     6001 cgtggcgatg gcgcatgccg acgcgctaga cgatttcgat ctggacatgt tgggggacgg
     6061 ggattccccg ggtccgggat ttacccccca cgactccgcc ccctacggcg ctctggatat
     6121 ggccgacttc gagtttgagc agatgtttac cgatgccctt ggaattgacg agtacggtgg
     6181 gtagtaataa gcgaatttct tatgatttat gatttttatt attaaataag ttataaaaaa
     6241 aataagtgta tacaaatttt aaagtgactc ttaggtttta aaacgaaaat tcttattctt
     6301 gagtaactct ttcctgtagg tcaggttgct ttctcaggta tagcatgaga tcgctcttat
     6361 tgaccacacc tctaccggca tgccgagcaa atgcctgcaa atcgctcccc ctcagcattt
     6421 aaatcctgca gggaattcct ttctgcatgc cacgttcgca gcgcctgatg cggtattttc
     6481 tccttacgca tctgtgcggt atttcacacc gcataatcgc tgggccattc tcatgaagaa
     6541 tatcttgaat ttattgtcat attactagtt ggtgtggaag tcctaatatc ggtgatcaat
     6601 atagtggttg acatgctggc tagtcaacat tgagcctttt gatcatgcaa atatattacg
     6661 gtattttaca atcaaatatc aaacttaact attgacttta taacttattt aggtggtaac
     6721 attcttataa aaaagaaaaa aattactgca aaacagtact agcttttaac ttgtatccta
     6781 ggttatctat gctgtctcac catagagaat attacctatt tcagaatgta tgtccatgat
     6841 tcgccgggta aatacatata atacacaaat ctggcttaat aaagtctata atatatctca
     6901 taaagaagtg ctaaattggc tagtgctata tatttttaag aaaatttctt ttgactaagt
     6961 ccatatcgac tttgtaaaag ttcacattag catacatata ttacacgagc cagaaatagt
     7021 aacttttgcc taaatcacaa attgcaaaat ttaattgctt gcaaaaggtc acatgcttat
     7081 aatcaacttt tttaaaaatt taaaatactt ttttattttt tatttttaaa cataaatgaa
     7141 ataatttatt tattgtttat gattaccgaa acataaaacc tgctcaagaa aaagaaactg
     7201 ttttgtcctt ggaaaaaaag cactacctag gagcggccaa aatgccgagg ctttcatagc
     7261 ttaaactctt tacagaaaat aggcattata gatcagttcg agttttctta ttcttccttc
     7321 cggttttatc gtcacagttt tacagtaaat aagtatcacc tcttagagtt cgatgataag
     7381 ctgtcaaaca tgagaattaa ttccacatgt taaaatagtg aaggagcatg ttcggcacac
     7441 agtggaccga acgtggggta agtgcactag ggtccggtta aacggatctc gcattgatga
     7501 ggcaacgcta attatcaaca tatagattgt tatctatctg catgaacacg aaatctttac
     7561 ttgacgactt gaggctgatg gtgtttatgc aaagaaacca ctgtgtttaa tatgtgtcac
     7621 tgtttgatat tactgtcagc gtagaagata atagtaaaag cggttaataa gtgtatttga
     7681 gataagtgtg ataaagtttt tacagcgaaa agacgataaa tacaagaaaa tgattacgag
     7741 gatacggaga gaggtatgta catgtgtatt tatatactaa gctgccggcg gttgtttgca
     7801 agaccgagaa aaggctagca agaatcgggt cattgtagcg tatgcgcctg tgaacattct
     7861 cttcaacaag tttgattcca ttgcggtgaa atggtaaaag tcaaccccct gcgatgtata
     7921 ttttcctgta caatcaatca aaaagccaaa tgatttagca ttatctttac atcttgttat
     7981 tttacagatt ttatgtttag atcttttatg cttgcttttc aaaaggcttg caggcaagtg
     8041 cacaaacaat acttaaataa atactactca gtaataacct atttcttagc atttttgacg
     8101 aaatttgcta ttttgttaga gtcttttaca ccatttgtct ccacacctcc gcttacatca
     8161 acaccaataa cgccatttaa tctaagcgca tcaccaacat tttctggcgt cagtccacca
     8221 gctaacataa aatgtaagct ctcggggctc tcttgccttc caacccagtc agaaatcgag
     8281 ttccaatcca aaagttcacc tgtcccacct gcttctgaat caaacaaggg aataaacgaa
     8341 tgaggtttct gtgaagctgc actgagtagt atgttgcagt cttttggaaa tacgagtctt
     8401 ttaataactg gcaaaccgag gaactcttgg tattcttgcc acgactcatc tccatgcagt
     8461 tggacgatcg atgataagct gtcaaacatg agaattgggt aataactgat ataattaaat
     8521 tgaagctcta atttgtgagt ttagtataca tgcatttact tataatacag ttttttagtt
     8581 ttgctggccg catcttctca aatatgcttc ccagcctgct tttctgtaac gttcaccctc
     8641 taccttagca tcccttccct ttgcaaatag tcctcttcca acaataataa tgtcagatcc
     8701 tgtagagacc acatcatcca cggttctata ctgttgaccc aatgcgtctc ccttgtcatc
     8761 taaacccaca ccgggtgtca taatcaacca atcgtaacct tcatctcttc cacccatgtc
     8821 tctttgagca ataaagccga taacaaaatc tttgtcgctc ttcgcaatgt caacagtacc
     8881 cttagtatat tctccagtag atagggagcc cttgcatgac aattctgcta acatcaaaag
     8941 gcctctaggt tcctttgtta cttcttctgc cgcctgcttc aaaccgctaa caatacctgg
     9001 gcccaccaca ccgtgtgcat tcgtaatgtc tgcccattct gctattctgt atacacccgc
     9061 agagtactgc aatttgactg tattaccaat gtcagcaaat tttctgtctt cgaagagtaa
     9121 aaaattgtac ttggcggata atgcctttag cggcttaact gtgccctcca tggaaaaatc
     9181 agtcaagata tccacatgtg tttttagtaa acaaattttg ggacctaatg cttcaactaa
     9241 ctccagtaat tccttggtgg tacgaacatc caatgaagca cacaagtttg tttgcttttc
     9301 gtgcatgata ttaaatagct tggcagcaac aggactagga tgagtagcag cacgttcctt
     9361 atatgtagct ttcgacatga tttatcttcg tttcctgcat gtttttgttc tgtgcagttg
     9421 ggttaagaat actgggcaat ttcatgtttc ttcaacacta catatgcgta tatataccaa
     9481 tctaagtctg tgctccttcc ttcgttcttc cttctgttcg gagattaccg aatcaaaaaa
     9541 atttcaagga aaccgaaatc aaaaaaaaga ataaaaaaaa aatgatgaat tgaaaagcta
     9601 attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
     9661 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     9721 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     9781 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     9841 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     9901 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     9961 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
    10021 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
    10081 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
    10141 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
    10201 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
    10261 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
    10321 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
    10381 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
    10441 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
    10501 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
    10561 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
    10621 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
    10681 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
    10741 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
    10801 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
    10861 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
    10921 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
    10981 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
    11041 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
    11101 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
    11161 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
    11221 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
    11281 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
    11341 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
    11401 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
    11461 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
    11521 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
    11581 gcagcgagtc agtgagcgag gaagcggaag a
//
