LOCUS       sequence-217438-        9910 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Beta-estradiol inducible expression vector; Z4 promoter-CYC1 
            terminator; artificial transcription factor under ACT1 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9910)
  AUTHORS   Patterson K, Shavarebi F, Magnan C, Chang I, Qi X, Baldi P, 
            Bilanchone V, Sandmeyer SB
  TITLE     Local features determine Ty3 targeting frequency at RNA polymerase 
            III transcription start sites.
  JOURNAL   Genome Res. 2019 Aug;29(8):1298-1309. doi: 10.1101/gr.240861.118. 
            Epub 2019 Jun 27.
  PUBMED    31249062
REFERENCE   2  (bases 1 to 9910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gr.240861.118"; journalName: "Genome Res"; date: "2019-08";
            volume: "29"; issue: "8"; pages: "1298-1309"
COMMENT     Sequence Label: pKP3915
FEATURES             Location/Qualifiers
     source          1..9910
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      133..382
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(149..167)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(428..451)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     promoter        1175..1518
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             1519..2328
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1586..1605)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     2954..2970
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     2955..2971
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      4490..4677
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(4545..4569)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     misc_feature    5377..6539
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN4 centromere fused to the 
                     autonomously replicating sequence ARS1/ARS416"
     rep_origin      5702..6539
                     /label=ARS1
                     /note="S. cerevisiae autonomously replicating sequence 
                     ARS1/ARS416"
     CDS             complement(6874..7677)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(7678..7893)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(7918..7936)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8004..8108
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8109..8177,8178..8969)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8327..8346)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9140..9728
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9629..9648
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9882..9899
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gttaacttaa ttaagtttaa actccggaca agatggcctt tggtgggttg aagaaggaaa
       61 aagacagaaa cgacttaatt acctacttga aaaaagcctg tgagtaaaca ggcccctttt
      121 cctttgtcga tatcatgtaa ttagttatgt cacgcttaca ttcacgccct cctcccacat
      181 ccgctctaac cgaaaaggaa ggagttagac aacctgaagt ctaggtccct atttattttt
      241 tttaatagtt atgttagtat taagaacgtt atttatattt caaatttttc ttttttttct
      301 gtacaaacgc gtgtacgcat gtaacattat actgaaaacc ttgcttgaga aggttttggg
      361 acgctcgaag gctttaattt gcaaagctta agagcgctgc gcgctacgta tatagttttt
      421 tctccttgac gttaaagtat agaggtatat taacaatttt ttgttgatac ttttatgaca
      481 tttgaataag aagtaataca aactgaaaat gttgaaagta ttagttaaag tggttatgca
      541 gcttttccat ttatatatct gttaatagat caaaaatcat cgcttcgctg attaattacc
      601 ccagaaataa ggctaaaaaa ctaatcgcat tatcatccta tggttgttaa tttgattcgt
      661 taatttgaag gtttgtgggg ccaggttact gccaattttt cctcttcata accataaaag
      721 ctagtattgt agaatcttta ttgttcggag cagtgcggcg cgaggcacat ctgcgtttca
      781 ggaacgcgac cggtgaagac gaggacgcac ggtctagact cctccgccgc actcctccgc
      841 cgctcctcct ccgccgcact cctccgccgc tcctcctccg ccgcactcct ccgccgcggc
      901 cgcacttcaa tatagcaatg agcagttaag cgtattactg aaagttccaa agagaaggtt
      961 tttttaggct aagataatgg ggctctttac atttccacaa catataagta agattagata
     1021 tggatatgta tatggatatg tatatggtgg taatgccatg taatatgatt attaaacttc
     1081 tttgcgtcca tccaaaaaaa aagtaagaat ttttgaaaat tcaatataaa gatctgttta
     1141 gcttgcctcg tccccgccgg gtcacccggc cagcgacatg gaggcccaga ataccctcct
     1201 tgacagtctt gacgtgcgca gctcaggggc atgatgtgac tgtcgcccgt acatttagcc
     1261 catacatccc catgtataat catttgcatc catacatttt gatggccgca cggcgcgaag
     1321 caaaaattac ggctcctcgc tgcagacctg cgagcaggga aacgctcccc tcacagacgc
     1381 gttgaattgt ccccacgccg cgcccctgta gagaaatata aaaggttagg atttgccact
     1441 gaggttcttc tttcatatac ttccttttaa aatcttgcta ggatacagtt ctcacatcac
     1501 atccgaacat aaacaaccat gggtaaggaa aagactcacg tttcgaggcc gcgattaaat
     1561 tccaacatgg atgctgattt atatgggtat aaatgggctc gcgataatgt cgggcaatca
     1621 ggtgcgacaa tctatcgatt gtatgggaag cccgatgcgc cagagttgtt tctgaaacat
     1681 ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg tcagactaaa ctggctgacg
     1741 gaatttatgc ctcttccgac catcaagcat tttatccgta ctcctgatga tgcatggtta
     1801 ctcaccactg cgatccccgg caaaacagca ttccaggtat tagaagaata tcctgattca
     1861 ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt
     1921 tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg
     1981 aataacggtt tggttgatgc gagtgatttt gatgacgagc gtaatggctg gcctgttgaa
     2041 caagtctgga aagaaatgca taagcttttg ccattctcac cggattcagt cgtcactcat
     2101 ggtgatttct cacttgataa ccttattttt gacgagggga aattaatagg ttgtattgat
     2161 gttggacgag tcggaatcgc agaccgatac caggatcttg ccatcctatg gaactgcctc
     2221 ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa aatatggtat tgataatcct
     2281 gatatgaata aattgcagtt tcatttgatg ctcgatgagt ttttctaatc agtactgaca
     2341 ataaaaagat tcttgttttc aagaacttgt catttgtata gtttttttat attgtagttg
     2401 ttctatttta atcaaatgtt agcgtgattt atattttttt tcgcctcgac atcatctgcc
     2461 cagatgcgaa gttaagtgcg ggccggcccg tacgctcacc ctaacatatt ttccaattaa
     2521 cctcaatatt tctctgtcac ccggcctcta ttttccattt tcttctttac ccgccacgcg
     2581 tttttttctt tcaaattttt ttcttccttc ttctttttct tccacgtcct cttgcataaa
     2641 taaataaacc gttttgaaac caaactcgcc tctctctctc ctttttgaaa tatttttggg
     2701 tttgtttgat cctttccttc ccaatctctc ttgtttaata tatattcatt tatatcacgc
     2761 tctcttttta tcttcctttt tttcctctct cttgtattct tccttcccct ttctactcaa
     2821 accaagaaga aaaagaaaag gtcaatcttt gttaaagaat aggatcttct actacatcag
     2881 cttttagatt tttcacgctt actgcttttt tcttcccaag atcgaaaatt tactgaatta
     2941 acagggcccc ccctcgaggt cgacggtatc gataagcttg aagcaagcct cctgaaagat
     3001 gggtacccgc ccatatgctt gccctgtcga gtcctgcgat cgccgctttt ctcgccacgc
     3061 caatcttacc cgccatatcc gcatccatac cggtcagaag cccttccagt gtcgaatctg
     3121 catgcgtaac ttcagtcgta atgcgaacct tgtgcgccac atccgcaccc acacaggatc
     3181 ccaaaagccg ttccaatgtc ggatctgtat gcggaacttt agtcgaaagg ccgacctgag
     3241 gcgtcacatt cgcacgcaca ccggcgagaa gccttttgcc tgtgacattt gtgggaggaa
     3301 gtttgccagg aagggcgacc tcaagaggca taccaaaatc catacaggtg gcggaggcac
     3361 acctgcagct gcgtcgactc tagaggatcc atctgctgga gacatgagag ctgccaacct
     3421 ttggccaagc ccgctcatga tcaaacgctc taagaagaac agcctggcct tgtccctgac
     3481 ggccgaccag atggtcagtg ccttgttgga tgctgagccc cccatactct attccgagta
     3541 tgatcctacc agacccttca gtgaagcttc gatgatgggc ttactgacca acctggcaga
     3601 cagggagctg gttcacatga tcaactgggc gaagagggtg ccaggctttg tggatttgac
     3661 cctccatgat caggtccacc ttctagaatg tgcctggcta gagatcctga tgattggtct
     3721 cgtctggcgc tccatggagc acccagtgaa gctactgttt gctcctaact tgctcttgga
     3781 caggaaccag ggaaaatgtg tagagggcat ggtggagatc ttcgacatgc tgctggctac
     3841 atcatctcgg ttccgcatga tgaatctgca gggagaggag tttgtgtgcc tcaaatctat
     3901 tattttgctt aattctggag tgtacacatt tctgtccagc accctgaagt ctctggaaga
     3961 gaaggaccat atccaccgag tcctggacaa gatcacagac actttgatcc acctgatggc
     4021 caaggcaggc ctgaccctgc agcagcagca ccagcggctg gcccagctcc tcctcatcct
     4081 ctcccacatc aggcacatga gtaacaaagg catggagcat ctgtacagca tgaagtgcaa
     4141 gaacgtggtg cccctctatg acctgctgct ggagatgctg gacgcccacc gcctacatgc
     4201 gcccactagc cgtggagggg catccgtgga ggagacggac caaagccact tggccactgc
     4261 gggctctact tcatcggagc tccacttaga cggcgaggac gtggcgatgg cgcatgccga
     4321 cgcgctagac gatttcgatc tggacatgtt gggggacggg gattccccgg gtccgggatt
     4381 taccccccac gactccgccc cctacggcgc tctggatatg gccgacttcg agtttgagca
     4441 gatgtttacc gatgcccttg gaattgacga gtacggtggg tagtaataag cgaatttctt
     4501 atgatttatg atttttatta ttaaataagt tataaaaaaa ataagtgtat acaaatttta
     4561 aagtgactct taggttttaa aacgaaaatt cttattcttg agtaactctt tcctgtaggt
     4621 caggttgctt tctcaggtat agcatgagat cgctcttatt gaccacacct ctaccggcat
     4681 gccgagcaaa tgcctgcaaa tcgctccccc tcagcattta aatcctgcag ggaattcctt
     4741 tctgcatgcc acgttcgcag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta
     4801 tttcacaccg cataatcgct gggccattct catgaagaat atcttgaatt tattgtcata
     4861 ttactagttg gtgtggaagt cctaatatcg gtgatcaata tagtggttga catgctggct
     4921 agtcaacatt gagccttttg atcatgcaaa tatattacgg tattttacaa tcaaatatca
     4981 aacttaacta ttgactttat aacttattta ggtggtaaca ttcttataaa aaagaaaaaa
     5041 attactgcaa aacagtacta gcttttaact tgtatcctag gttatctatg ctgtctcacc
     5101 atagagaata ttacctattt cagaatgtat gtccatgatt cgccgggtaa atacatataa
     5161 tacacaaatc tggcttaata aagtctataa tatatctcat aaagaagtgc taaattggct
     5221 agtgctatat atttttaaga aaatttcttt tgactaagtc catatcgact ttgtaaaagt
     5281 tcacattagc atacatatat tacacgagcc agaaatagta acttttgcct aaatcacaaa
     5341 ttgcaaaatt taattgcttg caaaaggtca catgcttata atcaactttt ttaaaaattt
     5401 aaaatacttt tttatttttt atttttaaac ataaatgaaa taatttattt attgtttatg
     5461 attaccgaaa cataaaacct gctcaagaaa aagaaactgt tttgtccttg gaaaaaaagc
     5521 actacctagg agcggccaaa atgccgaggc tttcatagct taaactcttt acagaaaata
     5581 ggcattatag atcagttcga gttttcttat tcttccttcc ggttttatcg tcacagtttt
     5641 acagtaaata agtatcacct cttagagttc gatgataagc tgtcaaacat gagaattaat
     5701 tccacatgtt aaaatagtga aggagcatgt tcggcacaca gtggaccgaa cgtggggtaa
     5761 gtgcactagg gtccggttaa acggatctcg cattgatgag gcaacgctaa ttatcaacat
     5821 atagattgtt atctatctgc atgaacacga aatctttact tgacgacttg aggctgatgg
     5881 tgtttatgca aagaaaccac tgtgtttaat atgtgtcact gtttgatatt actgtcagcg
     5941 tagaagataa tagtaaaagc ggttaataag tgtatttgag ataagtgtga taaagttttt
     6001 acagcgaaaa gacgataaat acaagaaaat gattacgagg atacggagag aggtatgtac
     6061 atgtgtattt atatactaag ctgccggcgg ttgtttgcaa gaccgagaaa aggctagcaa
     6121 gaatcgggtc attgtagcgt atgcgcctgt gaacattctc ttcaacaagt ttgattccat
     6181 tgcggtgaaa tggtaaaagt caaccccctg cgatgtatat tttcctgtac aatcaatcaa
     6241 aaagccaaat gatttagcat tatctttaca tcttgttatt ttacagattt tatgtttaga
     6301 tcttttatgc ttgcttttca aaaggcttgc aggcaagtgc acaaacaata cttaaataaa
     6361 tactactcag taataaccta tttcttagca tttttgacga aatttgctat tttgttagag
     6421 tcttttacac catttgtctc cacacctccg cttacatcaa caccaataac gccatttaat
     6481 ctaagcgcat caccaacatt ttctggcgtc agtccaccag ctaacataaa atgtaagctc
     6541 tcggggctct cttgccttcc aacccagtca gaaatcgagt tccaatccaa aagttcacct
     6601 gtcccacctg cttctgaatc aaacaaggga ataaacgaat gaggtttctg tgaagctgca
     6661 ctgagtagta tgttgcagtc ttttggaaat acgagtcttt taataactgg caaaccgagg
     6721 aactcttggt attcttgcca cgactcatct ccatgcagtt ggacgatcga tgataagctg
     6781 tcaaacatga gaattgggta ataactgata taattaaatt gaagctctaa tttgtgagtt
     6841 tagtatacat gcatttactt ataatacagt tttttagttt tgctggccgc atcttctcaa
     6901 atatgcttcc cagcctgctt ttctgtaacg ttcaccctct accttagcat cccttccctt
     6961 tgcaaatagt cctcttccaa caataataat gtcagatcct gtagagacca catcatccac
     7021 ggttctatac tgttgaccca atgcgtctcc cttgtcatct aaacccacac cgggtgtcat
     7081 aatcaaccaa tcgtaacctt catctcttcc acccatgtct ctttgagcaa taaagccgat
     7141 aacaaaatct ttgtcgctct tcgcaatgtc aacagtaccc ttagtatatt ctccagtaga
     7201 tagggagccc ttgcatgaca attctgctaa catcaaaagg cctctaggtt cctttgttac
     7261 ttcttctgcc gcctgcttca aaccgctaac aatacctggg cccaccacac cgtgtgcatt
     7321 cgtaatgtct gcccattctg ctattctgta tacacccgca gagtactgca atttgactgt
     7381 attaccaatg tcagcaaatt ttctgtcttc gaagagtaaa aaattgtact tggcggataa
     7441 tgcctttagc ggcttaactg tgccctccat ggaaaaatca gtcaagatat ccacatgtgt
     7501 ttttagtaaa caaattttgg gacctaatgc ttcaactaac tccagtaatt ccttggtggt
     7561 acgaacatcc aatgaagcac acaagtttgt ttgcttttcg tgcatgatat taaatagctt
     7621 ggcagcaaca ggactaggat gagtagcagc acgttcctta tatgtagctt tcgacatgat
     7681 ttatcttcgt ttcctgcatg tttttgttct gtgcagttgg gttaagaata ctgggcaatt
     7741 tcatgtttct tcaacactac atatgcgtat atataccaat ctaagtctgt gctccttcct
     7801 tcgttcttcc ttctgttcgg agattaccga atcaaaaaaa tttcaaggaa accgaaatca
     7861 aaaaaaagaa taaaaaaaaa atgatgaatt gaaaagctaa ttcttgaaga cgaaagggcc
     7921 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
     7981 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     8041 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     8101 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     8161 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     8221 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     8281 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     8341 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     8401 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     8461 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     8521 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     8581 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     8641 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     8701 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     8761 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     8821 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     8881 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     8941 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     9001 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     9061 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     9121 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     9181 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     9241 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     9301 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     9361 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     9421 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     9481 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     9541 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     9601 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     9661 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     9721 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     9781 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     9841 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     9901 aagcggaaga
//
