LOCUS       sequence-217466-        5336 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses aa572 to C-terminus of PhiC31 integrase fused to PYL CID.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5336)
  AUTHORS   Weinberg BH, Cho JH, Agarwal Y, Pham NTH, Caraballo LD, Walkosz M, 
            Ortega C, Trexler M, Tague N, Law B, Benman WKJ, Letendre J, Beal J,
            Wong WW
  TITLE     High-performance chemical- and light-inducible recombinases in 
            mammalian cells and mice.
  JOURNAL   Nat Commun. 2019 Oct 24;10(1):4845. doi: 10.1038/s41467-019-12800-7.
  PUBMED    31649244
REFERENCE   2  (bases 1 to 5336)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-10-24"; volume: "10(1):4845. doi"; pages: " 
            10.1038/s41467-019-12800-7"
COMMENT     Sequence Label: pBW1608_pCAG-PV1-PYL-L1-PhiC31-572C-BGHpA
FEATURES             Location/Qualifiers
     source          1..5336
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     466..488
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     550..569
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      671..680
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1217..1243
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1388..1408
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    1418..1642
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(1882..1901)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    1947..2002
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2001..2020)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(2363..2379)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2363..2379)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2376..2398)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2387..2403
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2411..2417,2418..2435,2436..2441))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2456..2477
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      2562..2696
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    2716..2850
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2753..2772)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2807..2826
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2918..2935)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3089..3677)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3169..3188)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3848..4639,4640..4708))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4471..4490
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4709..4813)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        4844..5223
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tgcacccccc tccccgagtt gctgagcacg gcccggcttc gggtgcgggg ctccgtacgg
       61 ggcgtggcgc ggggctcgcc gtgccgggcg gggggtggcg gcaggtgggg gtgccgggcg
      121 gggcggggcc gcctcgggcc ggggagggct cgggggaggg gcgcggcggc ccccggagcg
      181 ccggcggctg tcgaggcgcg gcgagccgca gccattgcct tttatggtaa tcgtgcgaga
      241 gggcgcaggg acttcctttg tcccaaatct gtgcggagcc gaaatctggg aggcgccgcc
      301 gcaccccctc tagcgggcgc ggggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg
      361 gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct ccctctccag cctcggggct
      421 gtccgcgggg ggacggctgc cttcgggggg gacggggcag ggcggggttc ggcttctggc
      481 gtgtgaccgg cggctctaga gcctctgcta accatgttca tgccttcttc tttttcctac
      541 agctcctggg caacgtgctg gttattgtgc tgtctcatca ttttggcaaa gaattgatta
      601 attcgagcga aggcgcgccc attactcgca tccattctca ggctgtctcg tctcgtctcg
      661 cacgcgtgcc gccaccatgg cgccaactca agacgagttc acccaactct cccaatcaat
      721 cgccgagttc cacacgtacc aactcggtaa cggccgttgc tcatctctcc tagctcagcg
      781 aatccacgcg ccgccggaaa cagtatggtc cgtggtgaga cgtttcgata ggccacagat
      841 ttacaaacac ttcatcaaaa gctgtaacgt gagtgaagat ttcgagatgc gagtgggatg
      901 cacgcgcgac gtgaacgtga taagtggatt accggcgaat acgtctcgag agagattaga
      961 tctgttggac gatgatcgga gagtgactgg gtttagtata accggtggtg aacataggct
     1021 gaggaattat aaatcggtta cgacggttca tagatttgag aaagaagaag aagaagaaag
     1081 gatctggacc gttgttttgg aatcttatgt tgttgatgta ccggaaggta attcggagga
     1141 agatacgaga ttgtttgctg atacggttat tagattgaat cttcagaaac ttgcttcgat
     1201 cactgaagct atgaactacc catacgatgt tccagattac gcttccggag ggtctggctc
     1261 cggatcaagt ggtggcagcg gtacccaggg cacccccatc gagaagagag ccagcatcac
     1321 ctgggccaag cctcccaccg acgacgacga ggatgacgcc caggacggca ccgaggacgt
     1381 ggccgccccc aagaaaaagc ggaaggtgtg agaattcctg tgccttctag ttgccagcca
     1441 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
     1501 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
     1561 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct
     1621 ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg
     1681 tatccccacg cgccctgtag cgtgtaccgc tgggagttcg tagacggaaa caaacgcaga
     1741 atccaagcgc ggccgcccag gatacataga ttaccacaac tccgagccct tccaccgcta
     1801 gcgcactcct caggtgcagg ctgcctatca gaaggtggtg gctggtgtgg ccaatgccct
     1861 ggctcacaaa taccactgag atctttttcc ctctgccaaa aattatgggg acatcatgaa
     1921 gccccttgag catctgactt ctggctaata aaggaaattt attttcattg caatagtgtg
     1981 ttggaatttt ttgtgtctct cactcggaag gacatatggg agggcaaatc atttaaaaca
     2041 tcagaatgag tatttggttt agagtttggc aacatatgcc catatgctgg ctgccatgaa
     2101 caaaggttgg ctataaagag gtcatcagta tatgaaacag ccccctgctg tccattcctt
     2161 attccataga aaagccttga cttgaggtta gatttttttt atattttgtt ttgtgttatt
     2221 tttttcttta acatccctaa aatttttctt acatgtttta ctagccagat ttttcctcct
     2281 ctcctgacta ctcccagtca tagctgtccc tcttctctta tggagatccc tcgacctgca
     2341 gcccaagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     2401 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     2461 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     2521 cgtgccagcg gatccgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
     2581 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     2641 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     2701 cttttgcaaa aagctaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     2761 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     2821 ctcatcaatg tatcttatca tgtctggatc cgctgcatta atgaatcggc caacgcgcgg
     2881 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     2941 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     3001 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     3061 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     3121 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     3181 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     3241 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     3301 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     3361 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     3421 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     3481 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     3541 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     3601 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     3661 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     3721 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     3781 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     3841 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     3901 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     3961 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     4021 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     4081 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     4141 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     4201 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     4261 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     4321 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     4381 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     4441 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     4501 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     4561 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     4621 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     4681 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     4741 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     4801 taggggttcc gcgcacattt ccccgaaaag tgccacctgg gtcgacattg attattgact
     4861 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
     4921 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
     4981 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
     5041 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
     5101 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
     5161 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
     5221 atggtcgagg tgagccccac gttctgcttc actctcccca tctccccccc ctccccaccc
     5281 ccaattttgt atttatttat tttttaatta ttttgtgcag cgatgggggc gggggg
//
