LOCUS       sequence-217477-        6007 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses aa234 to C-terminus of PhiC31 integrase fused to GAI CID.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6007)
  AUTHORS   Weinberg BH, Cho JH, Agarwal Y, Pham NTH, Caraballo LD, Walkosz M, 
            Ortega C, Trexler M, Tague N, Law B, Benman WKJ, Letendre J, Beal J,
            Wong WW
  TITLE     High-performance chemical- and light-inducible recombinases in 
            mammalian cells and mice.
  JOURNAL   Nat Commun. 2019 Oct 24;10(1):4845. doi: 10.1038/s41467-019-12800-7.
  PUBMED    31649244
REFERENCE   2  (bases 1 to 6007)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-10-24"; volume: "10(1):4845. doi"; pages: " 
            10.1038/s41467-019-12800-7"
COMMENT     Sequence Label: pBW2116_pCAG-PV1-GAI-L1-PhiC31-234C-NLS-BGHpA
FEATURES             Location/Qualifiers
     source          1..6007
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     466..488
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     550..569
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             689..706
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             2111..2131
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2141..2365
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(2605..2624)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    2670..2725
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2724..2743)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(3086..3102)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3086..3102)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3099..3121)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3110..3126
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3134..3140,3141..3158,3159..3164))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3179..3200
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      3285..3419
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    3439..3573
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3476..3495)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3530..3549
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3641..3658)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3812..4400)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3892..3911)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4571..5362,5363..5431))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5194..5213
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5432..5536)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        5567..5946
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tgcacccccc tccccgagtt gctgagcacg gcccggcttc gggtgcgggg ctccgtacgg
       61 ggcgtggcgc ggggctcgcc gtgccgggcg gggggtggcg gcaggtgggg gtgccgggcg
      121 gggcggggcc gcctcgggcc ggggagggct cgggggaggg gcgcggcggc ccccggagcg
      181 ccggcggctg tcgaggcgcg gcgagccgca gccattgcct tttatggtaa tcgtgcgaga
      241 gggcgcaggg acttcctttg tcccaaatct gtgcggagcc gaaatctggg aggcgccgcc
      301 gcaccccctc tagcgggcgc ggggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg
      361 gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct ccctctccag cctcggggct
      421 gtccgcgggg ggacggctgc cttcgggggg gacggggcag ggcggggttc ggcttctggc
      481 gtgtgaccgg cggctctaga gcctctgcta accatgttca tgccttcttc tttttcctac
      541 agctcctggg caacgtgctg gttattgtgc tgtctcatca ttttggcaaa gaattgatta
      601 attcgagcga aggcgcgccc attactcgca tccattctca ggctgtctcg tctcgtctcg
      661 cacgcgtgcc gccaccatga agcgggacca ccaccatcac catcatcagg acaagaaaac
      721 catgatgatg aacgaagagg acgacggcaa cggcatggac gagctgctgg ctgtgctggg
      781 ctacaaagtg cggagcagcg agatggccga cgtggcccag aaactggaac agctggaagt
      841 gatgatgagc aacgtgcagg aagatgacct gtcccagctg gccaccgaga cagtgcacta
      901 caaccccgcc gagctgtaca cctggctgga ctccatgctg accgacctga actccggagg
      961 gtctggctcc ggatcaagtg gtggcagcgg taccggcagc caggccgcca tccaccccgg
     1021 cagcatcacc ggcctgtgta agagaatgga cgccgacgcc gtgcccacca gaggcgagac
     1081 catcggcaag aaaaccgcca gcagcgcctg ggaccccgcc accgtgatga gaatcctgag
     1141 ggaccctagg atcgccggct tcgccgccga ggtgatctac aagaagaagc ccgacggcac
     1201 ccccaccacc aagatcgagg gctacagaat ccagagagac cccatcaccc tgagacctgt
     1261 ggagctggac tgtggcccta tcatcgagcc tgccgagtgg tacgagctgc aggcctggct
     1321 ggacggcaga ggcagaggca agggcctgag cagaggccag gccatcctga gcgccatgga
     1381 caagctgtac tgtgagtgtg gcgccgtgat gaccagcaag agaggcgagg agagcatcaa
     1441 ggacagctac cggtgccgga gaagaaaggt ggtggacccc agcgcccctg gccagcacga
     1501 gggcacctgt aatgtgagca tggccgccct ggacaagttc gtggccgagc ggatcttcaa
     1561 caagatccgg cacgccgagg gcgacgagga gaccctggcc ctgctgtggg aggccgccag
     1621 aagattcggc aagctgaccg aggcccccga gaagagcggc gagagggcca acctggtggc
     1681 cgagagagcc gacgccctga acgccctgga ggagctgtac gaggacagag ccgccggagc
     1741 ctatgacggc cctgtgggca ggaagcactt cagaaagcag caggccgccc tgaccctgag
     1801 acagcagggc gccgaggaaa gactggccga gctggaggcc gccgaggccc ctaagctgcc
     1861 cctggatcag tggttccccg aggatgccga cgccgacccc accggcccca agtcctggtg
     1921 gggcagagcc agcgtggacg acaagagggt gttcgtgggc ctgttcgtgg ataagatcgt
     1981 ggtgaccaag agcaccaccg gcaggggcca gggcaccccc atcgagaaga gagccagcat
     2041 cacctgggcc aagcctccca ccgacgacga cgaggatgac gcccaggacg gcaccgagga
     2101 cgtggccgcc cccaagaaaa agcggaaggt gtgagaattc ctgtgccttc tagttgccag
     2161 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact
     2221 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt
     2281 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat
     2341 gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg
     2401 gggtatcccc acgcgccctg tagcgtgtac cgctgggagt tcgtagacgg aaacaaacgc
     2461 agaatccaag cgcggccgcc caggatacat agattaccac aactccgagc ccttccaccg
     2521 ctagcgcact cctcaggtgc aggctgccta tcagaaggtg gtggctggtg tggccaatgc
     2581 cctggctcac aaataccact gagatctttt tccctctgcc aaaaattatg gggacatcat
     2641 gaagcccctt gagcatctga cttctggcta ataaaggaaa tttattttca ttgcaatagt
     2701 gtgttggaat tttttgtgtc tctcactcgg aaggacatat gggagggcaa atcatttaaa
     2761 acatcagaat gagtatttgg tttagagttt ggcaacatat gcccatatgc tggctgccat
     2821 gaacaaaggt tggctataaa gaggtcatca gtatatgaaa cagccccctg ctgtccattc
     2881 cttattccat agaaaagcct tgacttgagg ttagattttt tttatatttt gttttgtgtt
     2941 atttttttct ttaacatccc taaaattttc cttacatgtt ttactagcca gatttttcct
     3001 cctctcctga ctactcccag tcatagctgt ccctcttctc ttatggagat ccctcgacct
     3061 gcagcccaag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     3121 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     3181 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     3241 tgtcgtgcca gcggatccgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
     3301 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     3361 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     3421 aggcttttgc aaaaagctaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     3481 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     3541 aaactcatca atgtatctta tcatgtctgg atccgctgca ttaatgaatc ggccaacgcg
     3601 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     3661 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     3721 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     3781 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     3841 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     3901 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     3961 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     4021 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     4081 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     4141 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     4201 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     4261 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     4321 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     4381 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     4441 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     4501 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     4561 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     4621 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     4681 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     4741 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     4801 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     4861 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     4921 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     4981 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     5041 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     5101 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     5161 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     5221 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     5281 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     5341 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     5401 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     5461 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     5521 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgggtcgaca ttgattattg
     5581 actagttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc
     5641 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
     5701 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
     5761 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
     5821 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
     5881 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
     5941 accatggtcg aggtgagccc cacgttctgc ttcactctcc ccatctcccc cccctcccca
     6001 cccccaa
//
