LOCUS       sequence-217507-        5959 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses worm MPS-3 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5959)
  AUTHORS   Park KH, Hernandez L, Cai SQ, Wang Y, Sesti F
  TITLE     A family of K+ channel ancillary subunits regulate taste sensitivity
            in Caenorhabditis elegans.
  JOURNAL   J Biol Chem. 2005 Jun 10;280(23):21893-9. doi: 
            10.1074/jbc.M502732200. Epub 2005 Mar 30.
  PUBMED    15799965
REFERENCE   2  (bases 1 to 5959)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M502732200"; journalName: "J Biol Chem"; date: 
            "2005-06-10- 10"; volume: "280"; issue: "23"; pages: "21893-9"
COMMENT     Sequence Label: MPS-3
FEATURES             Location/Qualifiers
     source          1..5959
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..212
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     163..183
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     209..233
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          373..505
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     488..507
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        550..568
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    1146..1267
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1157..1176)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1211..1230
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      1453..1908
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1540..1559)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1750..1771
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2025..2382
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        2025..2216
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(2048..2068)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      2233..2368
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2433..3227
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2487..2506)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3097..3116
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    3291..3339
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(3380..3399)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3499..3521
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3559..3577)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3645..3749
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3750..3818,3819..4610)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3968..3987)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4781..5369
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5270..5289
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        5580..5959
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta caccaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctgcgatcgc ccgccccgtt gacgcaaatg ggcggtaggc
      181 gtgtacggtg ggaggtctat ataagcagag ctcgtttagt gaaccgtcag atcactagaa
      241 gctttattgc ggtagtttat cacagttaaa ttgctaacgc agtcagtgct tctgacacaa
      301 cagtctcgaa cttaagctgc agtgactctc ttaaggtagc cttgcagaag ttggtcgtga
      361 ggcactgggc aggtaagtat caaggttaca agacaggttt aaggagacca atagaaactg
      421 ggcttgtcga gacagagaag actcttgcgt ttctgatagg cacctattgg tcttactgac
      481 atccactttg cctttctctc cacaggtgtc cactcccagt tcaattacag ctcttaaggc
      541 tagagtactt aatacgactc actataggct agcctcgaga tgtccacctc cactcagcga
      601 ccaccggcgc caacttctgg ctcatcatcg acgccatgga ccaaagtagt gatattcata
      661 attattggca ttctcattgt agccttcatc gtgttcgccg ccgcttccat tgtgaaatat
      721 ataaaaagca agaagcaaca aaagcagcca aagaagaaag caaaaaagag cacaaaatcg
      781 cggagcaaga gtttgaagag caaacagtct gcagtaaccc caaaaaaaag agcatctcca
      841 attccacctc agccagctcc gccatcatct cccaaaccaa ctgaagaatc tgcgacacca
      901 tcctcctcat ttgctccgtc aactgagcca acaccgacag ttccagacaa ggttgatgat
      961 ggctccgagg acacggagca caaagagcaa gagcaacaga ctgttctgtg cacttatggc
     1021 aatggatgga agtctaagaa tcagcctccc cacgacttct ccttctcaat cgaatctgaa
     1081 taatctagag tcgacccggg cggccgcttc cctttagtga gggttaatgc ttcgagcaga
     1141 catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg
     1201 ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa
     1261 acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg agatgtggga
     1321 ggttttttaa agcaagtaaa acctctacaa atgtggtaaa atccgataag gatcgatccg
     1381 ggctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
     1441 aatggcgaat ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     1501 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     1561 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     1621 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
     1681 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     1741 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
     1801 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     1861 aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttcc tgatgcggta
     1921 ttttctcctt acgcatctgt gcggtatttc acaccgcata cgcggatctg cgcagcacca
     1981 tggcctgaaa taacctctga aagaggaact tggttaggta ccttctgagg cggaaagaac
     2041 cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
     2101 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
     2161 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
     2221 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     2281 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     2341 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagcttga ttcttctgac
     2401 acaacagtct cgaacttaag gctagagcca ccatgattga acaagatgga ttgcacgcag
     2461 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg
     2521 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca
     2581 agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc
     2641 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg
     2701 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg
     2761 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta
     2821 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag
     2881 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac
     2941 tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg
     3001 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg
     3061 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg
     3121 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg
     3181 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg
     3241 gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgatggccgc aataaaatat
     3301 ctttattttc attacatctg tgtgttggtt ttttgtgtga atcgatagcg ataaggatcc
     3361 gcgtatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac
     3421 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
     3481 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga
     3541 aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
     3601 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     3661 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     3721 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     3781 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     3841 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     3901 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     3961 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     4021 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     4081 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     4141 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     4201 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     4261 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     4321 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     4381 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     4441 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     4501 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     4561 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     4621 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     4681 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     4741 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     4801 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     4861 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     4921 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     4981 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     5041 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     5101 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     5161 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     5221 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     5281 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     5341 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     5401 tggccttttg ctggcctttt gctcacatgg ctcgacagat cttcaatatt ggccattagc
     5461 catattattc attggttata tagcataaat caatattggc tattggccat tgcatacgtt
     5521 gtatctatat cataatatgt acatttatat tggctcatgt ccaatatgac cgccatgttg
     5581 gcattgatta ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc
     5641 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
     5701 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
     5761 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
     5821 agtgtatcat atgccaagtc cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
     5881 gcattatgcc cagtacatga ccttacggga ctttcctact tggcagtaca tctacgtatt
     5941 agtcatcgct attaccatg
//
