LOCUS       sequence-217508-       18938 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  P element vector for transposon insertion of the 10Kb Drosophila 
            Amplicon in Follicle Cells (DAFC-62D) into Drosophila genome.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 18938)
  AUTHORS   Xie F, Orr-Weaver TL
  TITLE     Isolation of a Drosophila amplification origin developmentally 
            activated by transcription.
  JOURNAL   Proc Natl Acad Sci U S A. 2008 Jul 15;105(28):9651-6. doi: 
            10.1073/pnas.0804146105. Epub 2008 Jul 9.
  PUBMED    18621687
REFERENCE   2  (bases 1 to 18938)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.0804146105"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2008-07-15- 15"; volume: "105"; issue: "28"; pages: "9651-6"
COMMENT     Sequence Label: Yes-10kb
FEATURES             Location/Qualifiers
     source          1..18938
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    join(443..997,998..1028)
                     /label=P element 5' end
     rep_origin      complement(1263..1851)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1343..1362)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2022..2813,2814..2882))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2645..2664
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2883..2987)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3055..3073
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(3111..3133)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3233..3252
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     CDS             complement(join(3713..4327,4398..4529,4733..5048,5110..5764,
                     5839..6112,6514..6585))
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     primer_bind     7037..7055
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     primer_bind     7096..7112
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7096..7112
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     protein_bind    7161..7546
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     CDS             complement(12527..12538)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     protein_bind    17858..18243
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     misc_feature    join(18293..18494,18495..18525)
                     /label=P element 3' end
                     /note="P element 3' end"
ORIGIN
        1 ctttcgctgt ctcccacccg ctctccgcaa cacattcacc ttttgttcga cgaccttgga
       61 gcgactgtcg ttagttccgc gcgattcggt tcgctcaaat ggttccgagt ggttcatttc
      121 gtctcaatag aaattagtaa taaatatttg tatgtacaat ttatttgctc caatatattt
      181 gtatatattt ccctcacagc tatatttatt ctaatttaat attatgactt tttaaggtaa
      241 ttttttgtga cctgttcgga gtgattagcg ttacaatttg aactgaaagt gacatccagt
      301 gtttgttcct tgtgtagatg catctcaaaa aaatggtggg cataatagtg ttgtttatat
      361 atatcaaaaa taacaactat aataataaga atacatttaa tttagaaaat gcttggattt
      421 cactggaact agaattaatt cggctgctgc tctaaacgac gcatttcgta ctccaaagta
      481 cgaatttttt ccctcaagct cttattttca ttaaacaatg aacaggacct aacgcacagt
      541 cacgttattg tttacataaa tgattttttt tactattcaa acttactctg tttgtgtact
      601 cccactggta tagccttctt ttatcttttc tggttcaggc tctatcactt tactaggtac
      661 ggcatctgcg ttgagtcgcc tccttttaaa tgtctgacct tttgcaggtg cagccttcca
      721 ctgcgaatca ttaaagtggg tatcacaaat ttgggagttt tcaccaaggc tgcacccaag
      781 gctctgctcc cacaattttc tcttaatagc acacttcggc acgtgaatta attttactcc
      841 agtcacagct ttgcagcaaa atttgcaata tttcattttt ttttattcca cgtaagggtt
      901 aatgttttca aaaaaaaatt cgtccgcaca caacctttcc tctcaacaag caaacgtgca
      961 ctgaatttaa gtgtatactt cggtaagctt cggctatcga cgggaccacc ttatgttatt
     1021 tcatcatggg ccagacccac gtagtccagc ggcagatcgg cggcggagaa gttaagcgtc
     1081 tccaggatga ccttgcccga actggggcac gtggtgttcg acgatgtgca gctaatttcg
     1141 cccggctcca cgtccgccca ttggttaatc agcagaccct cgttggcgta acggaaccat
     1201 gagaggtacg acaaccattt gaggtatact ggcaccgagc ccgagttcaa gaagaaggcg
     1261 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     1321 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     1381 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     1441 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     1501 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     1561 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     1621 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     1681 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     1741 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     1801 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     1861 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     1921 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     1981 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     2041 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     2101 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     2161 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     2221 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     2281 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     2341 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     2401 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     2461 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     2521 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     2581 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     2641 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     2701 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     2761 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     2821 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     2881 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     2941 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     3001 aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg
     3061 cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
     3121 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc
     3181 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca
     3241 gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcaccg aatcgcgcgg
     3301 aactaacgac agtcgctcca aggtcgtcga acaaaaggtg aatgtgttgc ggagagcggg
     3361 tgggagacag cgaaagagca actacgaaac gtggtgtggt ggaggtgaat tatgaagagg
     3421 gcgcgcgatt tgaaaagtat gtatataaaa aatatatccc ggtgttttat gtagcgataa
     3481 acgagttttt gatgtaaggt atgcaggtgt gtaagtcttt tggttagaag acaaatccaa
     3541 agtctacttg tggggatgtt cgaaggggaa atacttgtat tctataggtc atatcttgtt
     3601 tttattggca caaatataat tacattagct ttttgagggg gcaataaaca gtaaacacga
     3661 tggtaataat ggtaaaaaaa aaaaacaagc agttatttcg gatatatgtc ggctactcct
     3721 tgcgtcgggc ccgaagtctt agagccagat atgcgagcac ccggaagctc acgatgagaa
     3781 tggccagacc cacgtagtcc agcggcagat cggcggcgga gaagttaagc gtctccagga
     3841 tgaccttgcc cgaactgggg cacgtggtgt tcgacgatgt gcagctaatt tcgcccggct
     3901 ccacgtccgc ccattggtta atcagcagac cctcgttggc gtaacggaac catgagaggt
     3961 acgacaacca tttgaggtat actggcaccg agcccgagtt caagaagaag ccgccaaaga
     4021 gcaggaatgg tatgataacc ggcggaccca cagacagcgc catcgaggtc gaggagctgg
     4081 cgcaggatat tagatatccg aaggacgttg acacattggc caccagagtg accagcgcca
     4141 ggcagttgaa gaagtgcagc actccggccc gcagtccgat catcggatag gcaatcgccg
     4201 tgaagaccag tggcactgtg agaaaaagcg gcaattcggc aatcgttttg cccagaaagt
     4261 atgtgtcaca gcgataaagt cgacttcggg cctccctcat aaaaactggc agctctgagg
     4321 tgaacaccta aatcgaatcg attcattaga aagttagtaa attattgaaa tgcaaatgta
     4381 ttctaaacat gacttacatt tatcgtggca aagacgtttt gaaaggtcat gttggtcagg
     4441 aagaggaaga tggctccgtt gatattcatc acacccactt gcgtgagttg ttggcccaaa
     4501 aagatgaggc caatcaagat ggcaaccatc tgcaaattaa aatgttactc gcatctcatt
     4561 aatattcgcg agttaaatga aatttattta tcttctgcaa aactataaac tatacatctc
     4621 attgaaaaaa actaagaagg gtgtggaatc aggcaattct atctaaaatc tagcgaattt
     4681 gtttccaaga attgtaagcg ttatatcatt tgtttccact ggaaccactc accgttgtct
     4741 gaataagtcg cacttttacg aggagtggtt ccttgagcac cgacagccag gatcgccaca
     4801 ggaccgcccg gaactgcatg aaccaggtgg ccttgtaggt gtacccattc tccggctgct
     4861 ccagtggctt ctccagattt ttggtggcca acaactgctc catatcccgg gctactttgc
     4921 taatggcaaa attgtcgcat atcttggcga tccgatcacg ggactcgatc tcccgtccgg
     4981 gcacaacggc caacacctgt acgtaaaagt ccgccggatt gtagttggta ggacactggg
     5041 cacccacgct ggataggagt tgagatgtta tgtaatacta gataccctta ataaacacat
     5101 cgaactcact aggaaaagaa gtcgacggct tcgctgggag tgcccaagaa agctaccctg
     5161 ccctcggcca tcagaaggat cttgtcaaag agctcaaaca gctcggaaga cggctgatga
     5221 atggtcagga tgacggtctt gcccttctgc gacagcttct tcagcacctg gacgacgctg
     5281 tgggcggtaa aggagtccag tccggaggtg ggctcatcgc agatcagaag cggcggatcg
     5341 gttagagcct cggaggcgaa tgccagacgc ttcctttctc cgccggacag acctttcacc
     5401 ctgccgggca caccgatgat cgtgtgctga catttgctga gcgaaagctc ctggatcacc
     5461 tgatccacgc gggccactcg ctgccgatag gtcagatgtc gtggcatccg caccatggct
     5521 tggaaaatca ggtgttccct ggccgttagg gagccgataa agaggtcatc ctgctggaca
     5581 taggcgcacc tggcctgcat ctccttggcg tccacaggtt ggccattgag cagtcgcatc
     5641 ccggatggcg atacttggat gccctgcggc gatcgaaagg caagggcatt cagcagggtc
     5701 gtctttccgg caccggaact gcccatcacg gccaaaagtt cgcccggata ggccacgccg
     5761 caaactgagt ttcaaattgg taattggacc ctttattaag atttcacaca gatcagccga
     5821 ctgcgaatag aaactcaccg ttcttgagca aatgtttcct gggcgccggt atgtgtcgct
     5881 cgttgcagaa tagtccgcgt gtccggttga ccagctgccg ccatccggag cccggctgat
     5941 tgaccgcccc aaagatgtcc atattgtgcc aggcataggt gaggttctcg gctagttggc
     6001 cgctccctga accggagtcc tccggcggac tgggtggccg gagcgtgccg tagtttttgg
     6061 cctgcccgaa gccctggtta atgcagctct gcgaagccgc tccgctgtca ccctgcaatg
     6121 ataggggatc tcaaatatca actacaagcg ttatgctcat ctaaccccga acaaaaagta
     6181 ccccgaagta tcctacgaag taggtttata cttttattta ttttttgtgc atctaggatc
     6241 agcttaaaat atctggttgt tatatttttt gtaaaaaaga atatagtcga aaatgaatgc
     6301 ctttagatgt cttgatcatg atatgatctc aaaaattgtc ttatatagcg agaacagcta
     6361 ccagaataat ctgtttcgtg tcactatttg tttgtgcaat tgcggtttgg gatttttgtg
     6421 ggtcgcagtt ctcacgccgc agacaatttg atgttgcaat cgcagttcct atagatcaag
     6481 tgaacttaag atgtatgcac atgtactact cacattgttc agatgctcgg cagatgggtg
     6541 tttgctgcct ccgcgaatta atagctcctg atcctcttgg cccattgccg ggatttttca
     6601 cactttcccc tgcttaccca cccaaaacca atcaccaccc caatcactca aaaaacaaac
     6661 aaaaataaga agcgagagga gttttggcac agcactttgt gtttaattga tggcgtaaac
     6721 cgcttggagc ttcgtcacga aaccgctgac aaaatgcaac tgaaggcgga cattgacgct
     6781 acgtaacgct acaaacggtg gcgaaagaga tagcggacgc agcggcgaaa gagacggcga
     6841 tatttctgtg gacagagaag gaggcaaaca gcgctgactt tgagtggaat gtcattttga
     6901 gtgagaggta atcgaaagaa cctggtacat caaataccct tggatcgaag taaatttaaa
     6961 actgatcaga taagttcaat gatatccagt gcagtaaaaa aaaaaaatgt tttttttatc
     7021 tactttccgc aaaaatgggt tttattaact tacatacata ctagaattcg gtacccgccc
     7081 ggggatcagg gccgctctag aactagtgga tcccccgggc tgcaggaatt cgatagctgg
     7141 atcctactgt tgccgagcac aattgatcgg ctaaatggta tggcaagaaa aggtatgcaa
     7201 tataataatc ttttattggg tatgcaacga aaatttgttt cgtcaacgta tgcaatattt
     7261 tttattaaaa gagggtatgc aatgtatttt attaaaaacg ggtatgcaat ataataatct
     7321 tttattgggt atgcaacgaa aatttgtttc gtcaaagtat gcaatatttt ttattaaaag
     7381 agggtatgca atgtatttta ttaaaaacgg gtatgcaatg aaaaattatt tggtttctct
     7441 aaaaagtatg cagcacttat tttttgataa ggtatgcaac aaaattttac tttgccgaaa
     7501 atatgcaatg tttttgcgaa taaattcaac gcacacttat tgctagcatg tttaatctcc
     7561 ttacagctgc ccatcatcaa atgaccatcg gcgggtcaac attttgataa cccattcatc
     7621 gggggtcaga ctggctgctc cttccgcatg gcggaatggg aagggtctgt ctataatgcc
     7681 aaattacatg cagattgaat ttttgtgaat tccgattcaa taaactgcca tgggtccaaa
     7741 ccccccttcc cacgatcgta atcccccgat ttatggcaca cacatgccca aaagcaggcg
     7801 gatgtgataa gtgctgtgat atgtgggcgg gattgtcagt cgggccgaat ctgtggataa
     7861 atatttatgt gctccaacat aaataaaagc aaagtatgca aatggaaatt tgacgatgct
     7921 aagactcttt tactctgcaa aaacagctta cagatacgat tatggcaaag cttgaaatca
     7981 gcaatcattt attcatctct agactttaaa tgattaagat atcaaaatcg accattcaca
     8041 ataaaattgt gtattgaaac tcaacaaatt tggcaacata ttccatggat agtgtatttg
     8101 tgtatttgtg ggaactgtcc gcactcattt ggaaatattt tctccttgat atcgacagat
     8161 gcgtttaaat ggcaaatgta aactcattga tagcacattg ggtgcaaaaa gtgaatgttt
     8221 catatgcaca cacagtagca gcgggttgcc aagagcaatt tgtcggatga attcaatgaa
     8281 ttcaattatt gcaaaacaat taaaccaaga tgggccactg gataaaggca ataatttatg
     8341 gcgattgcaa aaggttgaga gccaatgtgg acaactttaa gcagacgtaa acaaaacttg
     8401 gccgacgaaa gaaagccagc aaagtgaaag caaagcacat aaattaggcg agcggccaaa
     8461 aaattatggg aagattaact ttaagcgcag ctgatgactt tttaataccc tgtactacaa
     8521 ctgtaagccc ctgaaaactt tctgtgataa ccaatttaaa tcaaattacc tatatattac
     8581 ctatagctag ttaactataa ctatcataaa aatactattt taaatgacgt tattaatcca
     8641 ccctggagtt cagcatttcc agaaaaggtt ggtctgaatt tatgtgaaat tcccggtaaa
     8701 gaaaatcgct ttcctcagca caaagttaaa aaagcataaa cctcatttgg cccatttacc
     8761 aaaacaaatc aaacgtgacc acagacaatt ttttttttgt acatttttcc gctgctgcgg
     8821 gacaaaaaaa aagaacgaca aattattggc ttatttgcgt tttcaattgg atttttattg
     8881 gcacacatat aaaaagttga actttgcttt gttgcgcagc gcgttttaaa gaagtcgcac
     8941 ataatccccc gactttgcag atgacgcata cgccccgtta acgggccata aacaagaact
     9001 gtcaatgtaa tgtgtggaga acgtcctgcg gaatgtctgg ccatggtttt gccgagcagt
     9061 gagtggagtt tccccagcta atgggggtca gtcggcagtc gtccggcagt ctcttatcta
     9121 ccccccaaaa cttttttgtt gctgggaggt aagtgtttac ataaattatg tcagatgagc
     9181 agcccggaaa tgcttgtcat caattttttg cgctccatct atgtatgtat atgtatatat
     9241 gtgcgcttgt ctcgtataaa tcattctggt gggtggggaa aaagccaacc ggaatgcggg
     9301 tttcggctga caatttaaca tgccaaacac gttggttttg gtttggggcg attccgggtt
     9361 gtctgcccgt atcgagggcc ttaacctatt taggggcaat tatggtgatg gaaatggcga
     9421 aatggcccag gaattctgca gggaaatccc ggggatgctg agatttttct ttcactcctt
     9481 caatcactct ttatgggcca acttatggct ggcaaaatct cagattgagg tcattttgtt
     9541 gcccgcaatt aattagctaa aaatatcgcc accaatctgg agacacttgt tggggttccg
     9601 ttttcaattg actgcaataa ttgcaatgcc atttttatgg tcgattggat aattgcggga
     9661 ggaatgtgtc cttcgccttg gtcctttggc tccattcgaa aacttccctg gcttctgtcc
     9721 tgggaatggc tggcttttag ttatgtaatg gaattgaaac tattcaatta atgtatacaa
     9781 gttcatatta atagcacaag ttaatgattt aatttcagtc ataaatgtca ttagttttta
     9841 ccatcgttta ctggttaact cttgccaagg tcataaagtt cgcaaaaaat cagtttaaaa
     9901 gctaagaatt gggtgcgagg cacgttgccg tcttgtggtc aaattctatg ggctgttggg
     9961 ttatataagc acgccacttg gccaatttgg ggagcagaac gcgcacgtcg ctgccgcacc
    10021 attaacaatt gcttccacga tgagactgca cttggccctg atccttggat tttgcctagt
    10081 tggctgggcc cgctcgcagg gcaccaagta cggactgtgg actccggatc gggcgcactc
    10141 cgattctcag cccatccagt ggacgggcgg gcaattcgag ttcccatgcg ccagtaccaa
    10201 gtcgctcttc aagagctccg gaaagtttat acccaagaat gtgattgcca cgcgggcgca
    10261 gctcattgga gacaccatct atctggctct gccgcgctac aggaagggag ttccggccac
    10321 gctggtgaag accagtatca agccgggcac ctgctccacc acctttaagc cctatccctg
    10381 ctgggacctc caggaggagg gcaactgcaa ggctctgcaa tcggtggtgg atctggtggt
    10441 agaccaaaac gaggtgctct gggtcttgga cacgggcata gtgaacactc tggagactcc
    10501 ggtgcgcaag tgcccaccaa aagtggtggc catgtcggtg aagactggca aggttctgaa
    10561 gaccgtctcg ctggagggcc tgacttccag caattcccgt ctccaatatc tggtagtgga
    10621 ctatgctccg gatggcggct gctttgtcta cgtcagcgat gccgccaatc gggccatcat
    10681 cgtgtacaat ctccaggcgg atcggggatt cagggtggtg ctgcccaagg ccgttaccgc
    10741 cggctgtcgg tcgagggatg tcctctacat agccctcatc cgccgggact gcggctcaac
    10801 ggaactgtac ttcacctacc tgagcaccaa caaactgttc tccctgaagt cagagtatct
    10861 gaggagtgga gtggccgatg gacgcatctt gggtgagtta tatatttcga atttcaaaga
    10921 aaacatttat taagttatca taatattcag atctcggcaa gaagcccagt cgcatggtca
    10981 tcatcggcac ggacaacggc tcggcgatct tcttccgcaa cgagggcgac gcggaggtgt
    11041 accgctggga cacgaactcc acattcgtgg aggccaactt caagccggtt taccgcagtc
    11101 agacctgcca gctggtgacc catgctgtgc ccgattacaa gcggaacacg atgcgagtcc
    11161 tgcagagcaa ttttccggat tacatgcaga accgcgttgg atgcggagcc attcagcagc
    11221 tgggtctgat gcagggctgt tggtaaccat gttaacaccc cattttggcc accactcgtc
    11281 ctccacaagc caatccgaat tgaaagctga aaaactgaat atgccattgc agttagattg
    11341 gcaataaccg agaatgggtc gctctttgga ccctcgagtg ctcttcgtat ttggtattgg
    11401 tgttttgtac ataaactaac gactttggtc gctattgttg ttatctaagc taagcaaata
    11461 aacgtttcat attcatacag aaggagaggc taaataattg gggctactgt cgctgataaa
    11521 tggaatattt caatatgtaa attggtagtc cacatacact gtatatgcat ggatagcagt
    11581 ttttctcttt taatagcttt ttgcattgct tttattgcac tttattttac ctcttaaaaa
    11641 cttaatttta tttgttactg tttctggcaa ctttactctg ccataaaact tgggaacaac
    11701 agcgacatta attaagctaa ttggaactgg tcctcgtgtt aaagtagtca gtaaagtgca
    11761 cagattatac tgccattccc atatctcttc gcccctcact gacggaaaat cgagaggatc
    11821 gaacttcgta acctggagac gttgggtgtg gaaaaggcca ggcaagtgtt aattggaagt
    11881 tggctgataa attgcacggc atcccttgca gtggcttccg attccgtata tttcgtcgac
    11941 tcaatcggaa aaacagccac aaggcgccgc gggaaattgc gctcgatata aaatcaatta
    12001 atcagcaact cttgtctgtg cgctgcgagt gtgacctttg ccccgggggg ctgcgctatg
    12061 gcttcgtggc gtgttgacta cgcccactcg cagtctgaag cggatcacgc tgcagcccat
    12121 ggagccccaa taaatgtgcc aatgccaccc agctgagctc caaaaatacc aaacggaata
    12181 aaagtcaccg gaatcagctc caaccgatgg gagtggaagc agctgccacc gaaaaggccg
    12241 tgataaactt ctctgctctc gcttgattta caaatgctaa acgtgggtgt tttggccacc
    12301 aaagtgttca cttaacaacc aaaagaaaag agtgggatta gctccaataa gcctgcagct
    12361 ttgatactga atcaaaacta aagaaacaaa caggctaaac aattcaattt aaaatggcca
    12421 taacaatctc gcttggtaac caaacccaaa gtcaaagaaa gaaactggta ccaaaacaaa
    12481 caataattcg acctaattgg gtctgaaatg gaagagtatt tttgagttgc aactcccaga
    12541 ggtccgtagg tggaaatgac agataaaact ccaaaatgag tagctgcaaa actcattaac
    12601 ttttgaggtt ctgcgaataa aatcccttcc caaggggcat cacaaattat gccctttgat
    12661 gtgacaaaat acgcaataag tatatttttg tgtatttgtt gaggcaccga aaagataacc
    12721 ggagcccgca gacagtcagt tttaatgcct tctgtgtcaa aagcaaaaac cgaattctac
    12781 gaaagcggca aaaagacagt cgaaaggcag tcaaaggaac gtcaggaacg aaagcataag
    12841 tcctgacggg agtgacaaac gattttccgc tgcacaggtt tccccagcct catcggcctt
    12901 tcagtcgagg agatgcacaa aaaacccgcg tagtaattaa attatgcccc gaaaagccgt
    12961 atgaatattt taattcttcc tgcacacttt caaaggggcg cagccaagtg gaaagccctg
    13021 aagtttggga acttttaaag gccccttaaa tttgcacacc agaattattt cggtagcaca
    13081 agttaagctg gccaaataac attttcattg ggctttccgg gcatttcaga gatgtctaaa
    13141 gccactttaa agcctctgcc gccaggataa atcaactaaa tcaacttcaa tccgcaggag
    13201 attgacagac gagagagaga gagagagttc ttctgcttcg gcacgcaaac tgactgagga
    13261 actagggaca gggactgtca aaatgccacc accgaatccc cgaatacaaa aggacctcct
    13321 cggagccaaa ggagcggtgg gacactactg tgtggcgccc acttgaagtg ggtttgacag
    13381 ggaatggcag gcagcttccc ccaaatgctt tgatttcttt tgtgctccgc cgcctgaatg
    13441 ggacaaaaca ttcgaggcac cacttctccg gttaatcgaa aagcgagaaa atcgacagag
    13501 aatcgagctc tagtggaaaa atgaaagcaa atgaaagcgt gaacttcgtg ttcaattgcg
    13561 caaataaatc tgttaacaaa atatcaacaa ctttcgccgg cagctgtgcc acctccaagg
    13621 agcctttaaa gtctttcgag cagatagttt gtgttgccca aggtgatgtt gtatcttctt
    13681 taacactgca ctgaaacaaa tacactgtca attgtacaaa actgtagata cagcactttt
    13741 tatattttga tattttcagt aagttttttt tattggacgt aaacgaatag ctaatacaaa
    13801 gtgtaagtgc atattattcc ctgatttctc ctagtgtatg ttccctcaca ctgtctgtcg
    13861 ttcatgcatt tccatatgga ccaagaggat tatcgctacg ggttccaaaa aactgcccgc
    13921 aacgaaatcc tgttctgcgg ttgggctgcc atagcagcgg aagcttttcg ggctatgtcc
    13981 ctgtcaagtc agcacatcct gttcacccat tttccacgaa tttccccgag cctcgctcca
    14041 ctaacacgtc aacgcatctc catctccatc tctgtctccg catccatctc catcaccatc
    14101 ttcatgtgaa tctggatgga gagacagcgg actggacggg cggcgtttga gtgctatagg
    14161 attctattgg atatagttgg gccactcaac gctgctcccg aggcgagcgt taatgcaaag
    14221 tagctaactg gtttagagac acgcccccgg tgtgtatggc ggggggcggg gagtgggaag
    14281 tgggtaatgg gaaatggcca aagcgtgggc aaaaaatggg tgccaaacga cttccgacca
    14341 ggcttatttt caaggggtca cgtgctggca ggaaaatcaa caacaatggc agaatcatgc
    14401 tcaggcggaa aaacgggaat tttcttatgg gtttttcaag catacttata tagtttgtta
    14461 ttaatttaag cctgtttaaa taattgaatt gtagatgttt cgctatgaaa agttatgttt
    14521 tataactcat agcactaata aatataaaat ataatttgaa ttatgtgaca tttttcattt
    14581 actcatttaa ataaatattt ttcaatatta acattttggt atcatattta agattattta
    14641 actatttatt atttcctgta cacatttatc attttcggcc gaaggataat cgaagttctt
    14701 ttgtggtttg acggcaaatc gatgcaattg aaaaataatt gaacattgaa catcgaacgc
    14761 ttattgaatg tcatttggaa attcatggat gtgcgggcat gtgggcacca ggagaaggtt
    14821 aaggatggag gagcaggatc tgggcgggaa ctcgcatatc taatggatcc ccgccccgtg
    14881 caggcattcc acattgtcat cgtttcgcat tcctccggcc atgcaagatg ggaaaaagga
    14941 aaattattga tttggtttta acggcactta ggccctaata attgagatcc tcgcctttgg
    15001 cccacttttt tggcccggga aaagagacgt gtgagtcctg ggctggaaac gtcggagtcg
    15061 tgagcgaaat gtgacataaa ttgaagaatg atttggaaaa gggaaatctg tgttttcttt
    15121 tttttttggg aaagttaatg ctgccttcgg gggcttgtca atcagaaatc ttttcaaact
    15181 gaaacccaac gtggcaggaa tatataaacg aaagcgcgat ttaaatttta aacttaaact
    15241 ctctctcaat atacatccta atatcttctt taaagaagtg aacttagttg tagctaaaag
    15301 cgaaaaaaat aagttttaaa tcagaatctg tgattacttc tcacatggct tgtttttaca
    15361 atcaattctg tttggataaa acccacagaa attctagaag aaccatgtca taaactgctg
    15421 acgttaatca acatatgctt taaaataaaa tacagtgagt aagatggagg aagtaaattg
    15481 aaatgcaaac ataaatttcg ccaaatagat ccgcacataa atctaacatg aagcagttgg
    15541 gaaatgcgcc agatagaata aaaagtaaat aaataccagc cgaaataaga taaacgaacg
    15601 ttttacgagc actcgaaggg gattttaaac acattcgcag caggttctat taaaaagccg
    15661 ttgtagttgg gaaaactggg aaaattcgag gggttgggaa ggtggtcgtc gcagtttatc
    15721 atttcatttg atgatttatt acccatacgc cgaggggtcc gaaaaaaggg ttaactgcca
    15781 tatggactgg ctgaagctcc ataaatttga ccagcaaaaa ggttttggct aaatttattt
    15841 ttggcgcgct gaaagtcgat agaaaaccgt ttcattcata aaaacaaatc gaatgggatc
    15901 cactgcaaaa gcagcaaaag caaacggaaa agccaaactt ctgcaaagga aagaagaaaa
    15961 cattttacac tctctctctc tctctctgtt ttcaaggaaa ctcgtttttc cggttctctg
    16021 ttttgcacag actctgcaac ttcaacaagt cgtccagctg ttgccgtctg tggatggcaa
    16081 gtggaagctg ttttccgccc agctacacca gttgcttctg gtggaactcg ttttcccctg
    16141 gctttcgctg gcttttccga acagctgatt caacgctgtc gctgccggcg tcgacgccag
    16201 aaagctggca acactttcga caggcgcgca ggaaaagccg accgtcgatt tcctgagagc
    16261 tcggagaaaa tagcgagcag ctgccgctcg agtttttcgc agtttttcag ttggtttttc
    16321 attgcggtgc cgtacgcacg tgtctcggag ttttcccggc tgaaaatata attcgaaaac
    16381 gtcgaaaatg tgccgataag atgaaaaatc gcaataattg ttattttcgg tttagcgcgt
    16441 tgaattgaat ttttcgtaat ataagttttg cgatttgtgt aagttgccgt cacgaatttt
    16501 catcggctgg tcagtgaact tttggtcagt ttattggcat ttggtatcat ttcgcccaac
    16561 ttatatttca tttatggccg aaaaaaggtt tatctgatta gcataatggc cacaacatcc
    16621 atggggacac tgtgcctaat gtcctgaaaa ttgtattccc agaaaattgt ggtgtgtacc
    16681 agtgattgat gtaggtagcc cagttataag ttacgaagaa caaagaaaat tctggccaaa
    16741 tgattaagaa aggatgctta atagaaatct aaattcgttt taaatgagta aagtgaacct
    16801 gcagctaaac agatatttgt atcgtttagt aaactctttt gaagccctaa ttaaaggaat
    16861 ttttgcttga aaaagaaccc aaaggtattc agaatatggt aaatataatt atgaacttgg
    16921 tgctatgaat tatagaaact tttgttttta atttaattct gttctgtaag caacaacttt
    16981 ttgatttcaa atttcatgtc ggattctata tattttccgc acaataaaaa ctatttccga
    17041 aattattcgt accccttccc atcgtagaaa acttaagaaa ttaattgaag gcatgtggac
    17101 tttgcaacat gcaaccaaaa tgacacattt ttcttacaac tttctttcca cctatctgca
    17161 ctttcgggga actttactgc tctatttcgg caagaaatat attaaaggct ttttatgatc
    17221 aatgcgtccg caaaagtgca atccaagggc cagactcaac tggcaactgg ggagtacttt
    17281 ggaaaaaccg atggaaaacc tgcaaaggct catgccgaac gaacattaaa taaatgtgat
    17341 gctcgcatct tgtcaatgaa caaataaaca aataaaacgg aaatgggaat gggacaacaa
    17401 acattgtttg tacaaatgcg ggaaagtcgt aaaaagtgtt gtgagcatga aatttgaatt
    17461 tcgaagaaaa atatgggtaa agtcaagtaa ccgtggaggg aaacaaacct aaagtaaacc
    17521 tcaaacaaat gtatacaaaa attgttggca tttcgtgcta gattcctttt gggcttcttt
    17581 caactttgcg caatgcctgc tgctttttta tggccacaat aaataaaagt caactagagt
    17641 agaagggggg cactggcaaa caatggacaa aaggttggct tgcgaaaata tttgcacagt
    17701 cgcaaagtag caagcaaaaa atcaccaaac agtccagata gcgcaaagtt ttctaatttc
    17761 ccgcaaggaa atggctgcgc aaacctttcg aaaacatcca gtcatctcaa taggacacac
    17821 aatgcataaa actttcaatt tatggacatg cagagggcta gcaataagtg tgcgttgaat
    17881 ttattcgcaa aaacattgca tattttcggc aaagtaaaat tttgttgcat accttatcaa
    17941 aaaataagtg ctgcatactt tttagagaaa ccaaataatt tttcattgca tacccgtttt
    18001 taataaaata cattgcatac cctcttttaa taaaaaatat tgcatacttt gacgaaacaa
    18061 attttcgttg catacccaat aaaagattat tatattgcat acccgttttt aataaaatac
    18121 attgcatacc ctcttttaat aaaaaatatt gcatacgttg acgaaacaaa ttttcgttgc
    18181 atacccaata aaagattatt atattgcata ccttttcttg ccataccatt tagccgatca
    18241 attgtgctcg gcaacagtag gatccagcta tcgaattcct gcagccaagc tttgcgtact
    18301 cgcaaattat taaaaataaa actttaaaaa taatttcgtc taattaatat tatgagttaa
    18361 ttcaaacccc acggacatgc taagggttaa tcaacaatca tatcgctgtc tcactcagac
    18421 tcaatacgac actcagaata ctattccttt cactcgcact tattgcaagc atacgttaag
    18481 tggatgtctc ttgccgacgg gaccacctta tgttatttca tcatggtctg gccattctca
    18541 tcgtgagctt ccgggtgctc gcatatctgg ctctaagact tcgggcccga cgcaaggagt
    18601 agccgacata tatccgaaat aactgcttgt tttttttttt taccattatt accatcgtgt
    18661 ttactgttta ttgccccctc aaaaagctaa tgtaattata tttgtgccaa taaaaacaag
    18721 atatgaccta tagaatacaa gtatttcccc ttcgaacatc cccacaagta gactttggat
    18781 ttgtcttcta accaaaagac ttacacacct gcatacctta catcaaaaac tcgtttatcg
    18841 ctacataaaa caccgggata tattttttat atacatactt ttcaaatcgc gcgccctctt
    18901 cataattcac ctccaccaca ccacgtttcg tagttgct
//
