LOCUS       sequence-217517-         680 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive lentiviral expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 680)
  AUTHORS   Li J, Choi PS, Chaffer CL, Labella K, Hwang JH, Giacomelli AO, Kim 
            JW, Ilic N, Doench JG, Ly SH, Dai C, Hagel K, Hong AL, Gjoerup O, 
            Goel S, Ge JY, Root DE, Zhao JJ, Brooks AN, Weinberg RA, Hahn WC
  TITLE     An alternative splicing switch in FLNB promotes the mesenchymal cell
            state in human breast cancer.
  JOURNAL   Elife. 2018 Jul 30;7. pii: 37184. doi: 10.7554/eLife.37184.
  PUBMED    30059005
REFERENCE   2  (bases 1 to 680)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2018-07-30"; volume: "7. pii: 37184. doi"; pages: " 
            10.7554/eLife.37184"
COMMENT     Sequence Label: pLX317-QKI
FEATURES             Location/Qualifiers
     source          1..680
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(125..166)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    174..198
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tccagaggtt gattgtcgac ttaacgcgtg ggtgaagggt acacggagtc aggtcgtcct
       61 ttccacaaga tatataaagc caagaaatcg aaatactttc aagttacgga ctagttcatt
      121 actacgtaga atcgagaccg aggagagggt tagggatagg cttaccgata tcaaccactt
      181 tgtacaagaa agttgggtag ttgccggtgg cggctcggtc tgcggtcaca atcctttggt
      241 aaggatggac acgcatatcg tgccttcgaa ctttagtagc caccgcacct aatacaccac
      301 taggttcaat aggatactca aggattgatg tagctggtgc caatgtgtag gggtactcat
      361 agggtgtata gattaaacca gcttcgggcc ctggaggaac tattgcagca gttgggtgag
      421 gagttccgtt tggcatgaca gcggtctgta tttgtctgat caaaggcatt atggtagggc
      481 cagctggcgt aggagtacgc agggcagctg gtgggagaac cggcgcaggc ccagtaatga
      541 tccttggagc agcctgggct gttgctgcaa gagaaaaggc aagggctggt gatttaatgt
      601 tggcatctct gtaggtgcca ttcagaatcg caagctccat cagctgcatc ttcttcaggc
      661 tgtcttctcc ttctgctgca
//
