LOCUS       sequence-217533-         521 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive lentiviral expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 521)
  AUTHORS   Li J, Choi PS, Chaffer CL, Labella K, Hwang JH, Giacomelli AO, Kim 
            JW, Ilic N, Doench JG, Ly SH, Dai C, Hagel K, Hong AL, Gjoerup O, 
            Goel S, Ge JY, Root DE, Zhao JJ, Brooks AN, Weinberg RA, Hahn WC
  TITLE     An alternative splicing switch in FLNB promotes the mesenchymal cell
            state in human breast cancer.
  JOURNAL   Elife. 2018 Jul 30;7. pii: 37184. doi: 10.7554/eLife.37184.
  PUBMED    30059005
REFERENCE   2  (bases 1 to 521)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2018-07-30"; volume: "7. pii: 37184. doi"; pages: " 
            10.7554/eLife.37184"
COMMENT     Sequence Label: pXPR_BRD003-sgQKI-2204
FEATURES             Location/Qualifiers
     source          1..521
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        54..129
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    185..302
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ctttatatat cttgtggaaa ggacgaaaca ccggggagaa tccttggacc taggttttag
       61 agctagaaat agcaagttaa aataaggcta gtccgttatc aacttgaaaa agtggcaccg
      121 agtcggtgct tttttaagct tggcgtaact agatcttgag acaaatggca gtattcatcc
      181 acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca
      241 taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt
      301 ttcgggttta ttacagggac agcagagatc cactttggcg ccggctcgag ggggcccggg
      361 tgcaaagatg gataaagttt taaacagaga ggaatctttg cagctaatgg accttctagg
      421 tcttgaaagg agtgggaatt ggctccggtg cccgtcagtg ggcagagcgc acatcgccca
      481 cagtccccga gaagttgggg ggaggggtcg gcaattgatc c
//
