LOCUS       sequence-21755-e         784 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 784)
  AUTHORS   Boehm JS, Zhao JJ, Yao J, Kim SY, Firestein R, Dunn IF, Sjostrom SK,
            Garraway LA, Weremowicz S, Richardson AL, Greulich H, Stewart CJ, 
            Mulvey LA, Shen RR, Ambrogio L, Hirozane-Kishikawa T, Hill DE, Vidal
            M, Meyerson M, Grenier JK, Hinkle G, Root DE, Roberts TM, Lander ES,
            Polyak K, Hahn WC
  TITLE     Integrative Genomic Approaches Identify IKBKE as a Breast Cancer 
            Oncogene.
  JOURNAL   Cell. 2007 Jun 15. 129(6):1065-1079.
  PUBMED    17574021
REFERENCE   2  (bases 1 to 784)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2007 
            Jun 15. 129(6):1065-1079."
COMMENT     Sequence Label: pBabe-Neo-Myr-Flag-IKBKE
FEATURES             Location/Qualifiers
     source          1..784
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             74..97
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    104..128
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ccggatctat ggggtcttca aatctaaacc aaaggacccc agccagcgcc ggcgcaggat
       61 ccgaggttac cttgactaca aagacgatga cgacaagcaa ttgacaagtt tgtacaaaaa
      121 agttggcatg cagagcacag ccaattacct gtggcacaca gatgacctgc tggggcaggg
      181 ggccactgcc agtgtgtaca aggcccgcaa caagaaatcc ggagagctgg ttgctgtgaa
      241 ggtcttcaac actaccagct acctgcggcc ccgcgaggtg caggtgaggg agtttgaggt
      301 cctgcggaag ctgaaccacc agaacatcgt caagctcttt gcggtggagg agacgggcgg
      361 aagccggcag aaggtactgg tgatggagta ctgctccagt gggagcctgc tgagtgtgct
      421 ggagagccct gagaatgcct ttgggctgcc tgaggatgag ttcctggtgg tgctgcgctg
      481 tgtggtggcc ggcatgaacc acctgcggga gaacggcatt gtgcatcgcg acatcaagcc
      541 ggggaacatc atgcgcctcg taggggagga ggggcagagc atctacaagc tgacagactt
      601 cggcgctgcc cgggagctgg atgatgatga gaagttcgtc tcggtctatg ggactgagga
      661 gtacctgcat cccgacatgt atgagcgggc ggtgcttcga aagccccagc aaaaagcgtt
      721 cggggtgact gtggatctct ggagcattgg agtgaccttg taccatgcag ccactggcag
      781 cctg
//
