LOCUS       sequence-217556-        5917 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses N-terminus to aa323 of TP901 integrase fused to GID1 CID.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5917)
  AUTHORS   Weinberg BH, Cho JH, Agarwal Y, Pham NTH, Caraballo LD, Walkosz M, 
            Ortega C, Trexler M, Tague N, Law B, Benman WKJ, Letendre J, Beal J,
            Wong WW
  TITLE     High-performance chemical- and light-inducible recombinases in 
            mammalian cells and mice.
  JOURNAL   Nat Commun. 2019 Oct 24;10(1):4845. doi: 10.1038/s41467-019-12800-7.
  PUBMED    31649244
REFERENCE   2  (bases 1 to 5917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-10-24"; volume: "10(1):4845. doi"; pages: " 
            10.1038/s41467-019-12800-7"
COMMENT     Sequence Label: pSD31_pCAG-PV1-TP901-N323-L1-FRB-NLS-BGHpA
FEATURES             Location/Qualifiers
     source          1..5917
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     466..488
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     550..569
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             1688..1963
                     /codon_start=1
                     /product="T2098L mutant of FKBP-rapamycin binding domain of
                     human FRAP"
                     /label=FRB*
                     /note="binds synthetic ligands such as AP21967"
                     /translation="ILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTL
                     KETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLLQAWDLYYHVFRRIS"
     CDS             1964..1984
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    1994..2218
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(2458..2477)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    2523..2578
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2577..2596)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(2939..2955)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2939..2955)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2952..2974)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2963..2979
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2987..2993,2994..3011,3012..3017))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3032..3053
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      3138..3272
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    3292..3426
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3329..3348)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3383..3402
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3494..3511)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3665..4253)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3745..3764)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4424..5215,5216..5284))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5047..5066
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5285..5389)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        5420..5799
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tgcacccccc tccccgagtt gctgagcacg gcccggcttc gggtgcgggg ctccgtacgg
       61 ggcgtggcgc ggggctcgcc gtgccgggcg gggggtggcg gcaggtgggg gtgccgggcg
      121 gggcggggcc gcctcgggcc ggggagggct cgggggaggg gcgcggcggc ccccggagcg
      181 ccggcggctg tcgaggcgcg gcgagccgca gccattgcct tttatggtaa tcgtgcgaga
      241 gggcgcaggg acttcctttg tcccaaatct gtgcggagcc gaaatctggg aggcgccgcc
      301 gcaccccctc tagcgggcgc ggggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg
      361 gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct ccctctccag cctcggggct
      421 gtccgcgggg ggacggctgc cttcgggggg gacggggcag ggcggggttc ggcttctggc
      481 gtgtgaccgg cggctctaga gcctctgcta accatgttca tgccttcttc tttttcctac
      541 agctcctggg caacgtgctg gttattgtgc tgtctcatca ttttggcaaa gaattgatta
      601 attcgagcga aggcgcgccc attactcgca tccattctca ggctgtctcg tctcgtctcg
      661 cacgcgtgcc gccaccatga ccaagaaggt ggccatctac accagagtgt ccaccaccaa
      721 ccaggccgag gaaggcttca gcatcgacga gcagatcgac cggctgacca aatacgccga
      781 ggccatggga tggcaggtgt ccgataccta caccgacgcc ggctttagcg gcgccaagct
      841 ggaaagaccc gccatgcagc ggctgatcaa cgacatcgag aacaaggcct tcgacaccgt
      901 gctggtgtac aagctggaca ggctgagcag aagcgtgcgg gacaccctgt acctcgtgaa
      961 ggacgtgttc accaagaaca agatcgactt catcagcctg aacgagagca tcgacaccag
     1021 cagcgctatg ggcagcctgt tcctgaccat cctgagcgcc atcaacgagt tcgagcgcga
     1081 gaacatcaaa gaacggatga ccatgggcaa gctgggcaga gccaagagcg gcaagagcat
     1141 gatgtggacc aagaccgcct tcggctacta ccacaacaga aagaccggca tcctggaaat
     1201 agtgccactg caggccacca tcgtggaaca gatcttcacc gactacctga gcggcatctc
     1261 cctgaccaag ctgagagaca agctgaacga gtccggccac atcggcaagg acatcccttg
     1321 gagctaccgg accctgcggc agaccctgga caaccctgtg tactgcggct acatcaagtt
     1381 caaggactcc ctgttcgagg gcatgcacaa gcccatcatc ccttacgaga catacctgaa
     1441 ggtgcagaaa gagctggaag agagacagca gcagacctac gagcggaaca acaaccccag
     1501 acccttccag gccaagtaca tgctgtccgg catggccaga tgcggctact gtggcgcccc
     1561 tctgaagatc gtgctgggcc acaagagaaa ggacggcagc cggaccatga agtaccactg
     1621 cgccaaccgg ttccctagaa agacctccgg agggtctggc tccggatcaa gtggtggcag
     1681 cggtaccatc ctctggcatg agatgtggca tgaaggcctg gaagaggcat ctcgtttgta
     1741 ctttggggaa aggaacgtga aaggcatgtt tgaggtgctg gagcccttgc atgctatgat
     1801 ggaacggggc ccccagactc tgaaggaaac atcctttaat caggcctatg gtcgagattt
     1861 aatggaggcc caagagtggt gcaggaagta catgaaatca gggaatgtca aggacctcct
     1921 ccaagcctgg gacctctatt atcatgtgtt ccgacgaatc tcacccaaga aaaagcggaa
     1981 ggtgtgagaa ttcctgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg
     2041 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt
     2101 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc
     2161 aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct
     2221 tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcgtg
     2281 taccgctggg agttcgtaga cggaaacaaa cgcagaatcc aagcgcggcc gcccaggata
     2341 catagattac cacaactccg agcccttcca ccgctagcgc actcctcagg tgcaggctgc
     2401 ctatcagaag gtggtggctg gtgtggccaa tgccctggct cacaaatacc actgagatct
     2461 ttttccctct gccaaaaatt atggggacat catgaagccc cttgagcatc tgacttctgg
     2521 ctaataaagg aaatttattt tcattgcaat agtgtgttgg aattttttgt gtctctcact
     2581 cggaaggaca tatgggaggg caaatcattt aaaacatcag aatgagtatt tggtttagag
     2641 tttggcaaca tatgcccata tgctggctgc catgaacaaa ggttggctat aaagaggtca
     2701 tcagtatatg aaacagcccc ctgctgtcca ttccttattc catagaaaag ccttgacttg
     2761 aggttagatt ttttttatat tttgttttgt gttatttttt tctttaacat ccctaaaatt
     2821 ttccttacat gttttactag ccagattttt cctcctctcc tgactactcc cagtcatagc
     2881 tgtccctctt ctcttatgga gatccctcga cctgcagccc aagcttggcg taatcatggt
     2941 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     3001 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     3061 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagcggatc cgcatctcaa
     3121 ttagtcagca accatagtcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     3181 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     3241 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc taacttgttt
     3301 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     3361 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     3421 tggatccgct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     3481 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     3541 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     3601 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     3661 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     3721 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     3781 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     3841 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     3901 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     3961 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     4021 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     4081 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     4141 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     4201 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     4261 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     4321 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     4381 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     4441 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     4501 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     4561 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     4621 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     4681 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     4741 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     4801 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     4861 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     4921 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     4981 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     5041 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     5101 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     5161 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     5221 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     5281 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     5341 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     5401 gaaaagtgcc acctgggtcg acattgatta ttgactagtt attaatagta atcaattacg
     5461 gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc
     5521 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc
     5581 atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact
     5641 gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat
     5701 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact
     5761 tggcagtaca tctacgtatt agtcatcgct attaccatgg tcgaggtgag ccccacgttc
     5821 tgcttcactc tccccatctc ccccccctcc ccacccccaa ttttgtattt atttattttt
     5881 taattatttt gtgcagcgat gggggcgggg ggggggg
//
