LOCUS       sequence-217559-       10381 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Targeted DNA methylation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10381)
  AUTHORS   Lu Z, Liu Z, Mao W, Wang X, Zheng X, Chen S, Cao B, Huang S, Zhang 
            X, Zhou T, Zhang Y, Huang X, Sun Q, Li JD
  TITLE     Locus-specific DNA methylation of Mecp2 promoter leads to 
            autism-like phenotypes in mice.
  JOURNAL   Cell Death Dis. 2020 Feb 3;11(2):85. doi: 10.1038/s41419-020-2290-x.
  PUBMED    32015323
REFERENCE   2  (bases 1 to 10381)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Death 
            Dis."; date: "2020-02-3"; volume: "11(2):85. doi"; pages: " 
            10.1038/s41419-020-2290-x"
COMMENT     Sequence Label: 
            pSt1374m-EF1alpha-N-NLS-DNMT3L-L-DNMT3Amut-L-dcas9-NLS
FEATURES             Location/Qualifiers
     source          1..10381
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        10..1191
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          241..1183
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1140..1160
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     1241..1260
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1241..1259
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1288..1308
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1963..1980
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             6997..7017
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7048..7104
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             7105..7815
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(7250..7268)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     7518..7537
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(7835..7852)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7841..8065
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(8227..8244)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8398..8986)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8478..8497)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9157..9948,9949..10017))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9780..9799
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10018..10122)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10197..10216)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 cgcgtgtgag gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag
       61 aagttggggg gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg cggggtaaac
      121 tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat
      181 ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag
      241 gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg cccttgcgtg
      301 ccttgaatta cttccacgcc cctggctgca gtacgtgatt cttgatcccg agcttcgggt
      361 tggaagtggg tgggagagtt cgaggccttg cgcttaagga gccccttcgc ctcgtgcttg
      421 agttgaggcc tggcctgggc gctggggccg ccgcgtgcga atctggtggc accttcgcgc
      481 ctgtctcgct gctttcgata agtctctagc catttaaaat ttttgatgac ctgctgcgac
      541 gctttttttc tggcaagata gtcttgtaaa tgcgggccaa gatctgcaca ctggtatttc
      601 ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc cagcgcacat gttcggcgag
      661 gcggggcctg cgagcgcggc caccgagaat cggacggggg tagtctcaag ctggccggcc
      721 tgctctggtg cctggcctcg cgccgccgtg tatcgccccg ccctgggcgg caaggctggc
      781 ccggtcggca ccagttgcgt gagcggaaag atggccgctt cccggccctg ctgcagggag
      841 ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt gagtcaccca cacaaaggaa
      901 aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc acggagtacc gggcgccgtc
      961 caggcacctc gattagttct cgagcttttg gagtacgtcg tctttaggtt ggggggaggg
     1021 gttttatgcg atggagtttc cccacactga gtgggtggag actgaagtta ggccagcttg
     1081 gcacttgatg taattctcct tggaatttgc cctttttgag tttggatctt ggttcattct
     1141 caagcctcag acagtggttc aaagtttttt tcttccattt caggtgtcgt ggagctctct
     1201 ggctaactag agaacccact gcttactggc ttatcgaaat taatacgact cactataggg
     1261 agacccaagc tggctagcac catgggacct aagaaaaaga ggaaggtggc ggccgctggc
     1321 ggcagcatgt tcgaaaccgt gcctgtgtgg aggagacagc cagtccgggt gctgtccctt
     1381 tttgaagaca tcaagaaaga gctgacgagt ttgggctttt tggaaagtgg ttctgacccg
     1441 ggacaactga agcatgtggt tgatgtcaca gacacagtga ggaaggatgt ggaggagtgg
     1501 ggacccttcg atcttgtgta cggcgccaca cctcccctgg gccacacctg tgaccgtcct
     1561 cccagctggt acctgttcca gttccaccgg ctcctgcagt acgcacggcc caagccaggc
     1621 agccccaggc ccttcttctg gatgttcgtg gacaatctgg tgctgaacaa ggaagacctg
     1681 gacgtcgcat ctcgcttcct ggagatggag ccagtcacca tcccagatgt ccacggcgga
     1741 tccttgcaga atgctgtccg cgtgtggagc aacatcccag ccataaggag cagcaggcac
     1801 tgggctctgg tttcggaaga agaattgtcc ctgctggccc agaacaagca gagctcgaag
     1861 ctcgcggcca agtggcccac caagctggtg aagaactgct ttctccccct aagagaatat
     1921 ttcaagtatt tttcaagcag cgggcgcagc ttcagcagcg gactagtgcc ccgcggcagc
     1981 cacccagctg agaagaggaa gcccatccgg gtgctgtctc tctttgatgg aatcgctaca
     2041 gggctcctgg tgctgaagga cttgggcatt caggtggacc gctacattgc ctcggaggtg
     2101 tgtgaggact ccatcacggt gggcatggtg cggcaccagg ggaagatcat gtacgtcggg
     2161 gacgtccgca gcgtcacaca gaagcatatc caggagtggg gcccattcga tctggtgatt
     2221 gggggcagtc cctccaatga cctctccatc gtcaaccctg ctcgcaaggg cctctacgag
     2281 ggcactggcc ggctcttctt tgagttctac cgcctcctgc atgatgcgcg gcccaaggag
     2341 ggagatgatc gccccttctt ctggctcttt gagaatgtgg tggccatggg cgttagtgac
     2401 aagagggaca tctcgcgatt tctcgagtcc aaccctgtga tgattgatgc caaagaagtg
     2461 tcagctgcac acagggcccg ctacttctgg ggtaaccttc ccggtatgaa caggccgttg
     2521 gcatccactg tgaatgataa gctggagctg caggagtgtc tggagcatgg caggatagcc
     2581 aagttcagca aagtgaggac cattactacg aggtcaaact ccataaagca gggcaaagac
     2641 cagcattttc ctgtcttcat gaatgagaaa gaggacatct tatggtgcac tgaaatggaa
     2701 agggtatttg gtttcccagt ccactatact gacgtctcca acatgagccg cttggcgagg
     2761 cagagactgc tgggccggtc atggagcgtg ccagtcatcc gccacctctt cgctccgctg
     2821 aaggagtatt ttgcgtgtgt gagcggcagc gagacccccg gtaccagcga gagcgccacc
     2881 cccgagagcg acaagaaata ctctattgga ctggctatcg ggacaaactc cgttggctgg
     2941 gccgtcataa ccgacgagta taaggtgcca agcaagaaat tcaaggtgct gggtaatact
     3001 gaccgccatt caatcaagaa gaacctgatc ggagcactcc tcttcgactc cggtgaaacc
     3061 gctgaagcta ctcggctgaa gcggaccgca aggcggagat acacccgccg caagaatcgg
     3121 atatgttatc tgcaagagat ctttagcaac gaaatggcta aggtggacga ctccttcttt
     3181 caccgcctgg aagagagctt tctggtggag gaggataaga aacacgagag gcaccctata
     3241 ttcggaaata tcgtggatga ggtggcttac catgaaaagt atcctacaat ctaccatctg
     3301 aggaagaagc tggtggacag caccgataaa gcagacctga ggctcatcta tctggccctg
     3361 gctcatatga taaagtttag aggacacttt ctgatcgagg gcgacctgaa tcccgataat
     3421 tccgatgtgg ataaactctt cattcaactg gtgcagacat ataaccaact gttcgaggag
     3481 aatcccataa acgcttctgg tgtggatgcc aaggctattc tgtccgctcg gctgtccaag
     3541 tcacgcagac tggagaatct gattgcccaa ctgccaggag aaaagaagaa cggcctgttt
     3601 gggaacctca tcgccctgag cctgggcctg acacctaact tcaagtccaa ttttgatctg
     3661 gccgaagatg ctaaactcca gctctccaag gacacctatg acgatgatct ggacaacctg
     3721 ctcgcacaga taggcgacca gtacgccgat ctctttctgg ctgctaagaa tctctccgac
     3781 gccattctgc tgagcgacat actccgggtc aacactgaga tcaccaaagc acctctgagc
     3841 gcctccatga taaaacgcta tgatgaacac catcaagacc tgactctgct caaagccctc
     3901 gtgaggcaac agctgccaga gaagtacaaa gagatattct tcgaccagag caagaatgga
     3961 tatgccggat acatcgatgg cggagcatca caggaagaat tttacaagtt catcaaacca
     4021 atcctcgaga agatggacgg tactgaagag ctgctggtga agctgaacag ggaggacctg
     4081 ctgaggaagc agaggacctt tgataatggc tccattccac atcagataca cctgggagag
     4141 ctgcatgcaa tcctccgcag gcaggaggat ttctatcctt tcctgaagga taaccgggag
     4201 aagatagaga agatcctgac cttcaggatc ccttattacg tcggccctct ggctagaggc
     4261 aactcccgct tcgcttggat gaccaggaaa tctgaggaga caattactcc ttggaacttc
     4321 gaagaggtcg tggataaggg cgcaagcgcc cagtcattca tcgaacggat gaccaatttc
     4381 gataagaacc tgccaaacga gaaggtcctg cccaaacatt cactcctgta cgagtatttc
     4441 accgtctata acgagctgac taaagtgaag tacgtgaccg agggcatgag gaagcctgcc
     4501 ttcctgtccg gagagcagaa gaaggctatc gttgatctgc tcttcaagac taatagaaag
     4561 gtgacagtga agcagctcaa ggaggattac tttaagaaga tcgaatgctt tgactcagtg
     4621 gaaatctctg gcgtggagga ccgctttaat gccagcctgg gcacttacca tgatctgctg
     4681 aagataatca aagacaaaga tttcctcgat aatgaggaga acgaggacat cctggaagat
     4741 atcgtgctga ccctgactct gttcgaggat agagagatga tcgaagagcg cctgaagacc
     4801 tatgcccatc tgtttgacga taaagtcatg aaacagctca agcggcggcg ctacactggg
     4861 tggggtagac tctccaggaa actcataaac ggcatccgcg acaaacagag cggaaagacc
     4921 atcctggatt tcctgaaatc cgacggattc gctaacagga acttcatgca actgattcac
     4981 gatgactctc tgacatttaa agaggacatc cagaaggcac aggtgagcgg tcaaggcgac
     5041 agcctgcacg agcacatcgc caacctcgct ggatcacccg ccataaagaa gggaatactg
     5101 cagacagtca aggtcgtgga cgaactcgtc aaagtgatgg gtcggcacaa gccagagaat
     5161 atcgttatcg aaatggcaag ggagaaccaa accacccaga agggccagaa gaactctcgg
     5221 gaacggatga aaagaatcga agagggaatt aaggagctgg gatctcagat actgaaggag
     5281 caccctgtgg agaatacaca gctccagaac gagaaactct acctgtacta cctccagaac
     5341 gggcgggaca tgtacgttga ccaggaactc gacatcaacc ggctgtccga ttatgacgtg
     5401 gacgctattg ttccacagtc cttcctcaaa gatgactcca ttgacaacaa ggtgctgacc
     5461 agatccgata aggcccgcgg taagtctgac aatgttccat cagaagaggt ggtcaagaag
     5521 atgaagaatt actggcggca gctcctcaac gccaaactga tcacccagcg gaagtttgac
     5581 aatctgacta aggcagaaag aggaggtctg agcgaactcg acaaggccgg ctttattaag
     5641 aggcaactgg tcgaaacacg ccagattacc aaacacgtgg cacaaatcct cgactctagg
     5701 atgaacacta agtacgatga gaacgataag ctgatcaggg aagtgaaagt gataactctg
     5761 aagagcaagc tggtgtctga cttccggaag gactttcaat tctacaaagt tcgcgaaata
     5821 aacaattacc atcatgctca cgatgcctat ctcaatgctg tcgttggcac cgccctgatc
     5881 aagaaatacc ctaaactgga gtctgagttc gtgtacggtg actataaagt ctacgatgtg
     5941 aggaagatga tagcaaagtc tgagcaagag attggcaaag ccaccgccaa gtacttcttc
     6001 tactctaata tcatgaattt ctttaagact gagataaccc tggctaacgg cgaaatccgg
     6061 aagcgcccac tgatcgaaac aaacggagaa acaggagaaa tcgtgtggga taaaggcagg
     6121 gacttcgcaa ctgtgcggaa ggtgctgtcc atgccacaag tcaatatcgt gaagaagacc
     6181 gaagtgcaga ccggcggatt ctcaaaggag agcatcctgc caaagcggaa ctctgacaag
     6241 ctgatcgcca ggaagaaaga ttgggaccca aagaagtatg gcggtttcga ttcccctaca
     6301 gtggcttatt ccgttctggt cgtggcaaaa gtggagaaag gcaagtccaa gaaactcaag
     6361 tctgttaagg agctgctcgg aattactatt atggagagat ccagcttcga gaagaatcca
     6421 atcgatttcc tggaagctaa gggctataaa gaagtgaaga aagatctcat catcaaactg
     6481 cccaagtact ctctctttga gctggagaat ggtaggaagc ggatgctggc ctccgccgga
     6541 gagctgcaga aaggaaacga gctggctctg ccctccaaat acgtgaactt cctgtatctg
     6601 gcctcccact acgagaaact caaaggtagc cctgaagaca atgagcagaa gcaactcttt
     6661 gttgagcaac ataaacacta cctggacgaa atcattgaac agattagcga gttcagcaag
     6721 cgggttattc tggccgatgc aaacctcgat aaagtgctga gcgcatataa taagcacagg
     6781 gacaagccaa ttcgcgaaca agcagagaat attatccacc tctttactct gactaatctg
     6841 ggcgctcctg ctgccttcaa gtatttcgat acaactattg acaggaagcg gtacacctct
     6901 accaaagaag ttctcgatgc caccctgata caccagtcaa ttaccggact gtacgagact
     6961 cgcatcgacc tgtctcagct cggcggcgac ggttctccca agaagaagag gaaagtctcg
     7021 agcggtggag ctgcaggagg atccggcgcc acgaacttct ctctgttaaa gcaagcagga
     7081 gacgtggaag aaaaccccgg tcctatggtg agcaagggcg aggaggataa catggccatc
     7141 atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg ccacgagttc
     7201 gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag
     7261 gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca gttcatgtac
     7321 ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttgaa gctgtccttc
     7381 cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg
     7441 acccaggact cctccctgca ggacggcgag ttcatctaca aggtgaagct gcgcggcacc
     7501 aacttcccct ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctcctcc
     7561 gagcggatgt accccgagga cggcgccctg aagggcgaga tcaagcagag gctgaagctg
     7621 aaggacggcg gccactacga cgctgaggtc aagaccacct acaaggccaa gaagcccgtg
     7681 cagctgcccg gcgcctacaa cgtcaacatc aagttggaca tcacctccca caacgaggac
     7741 tacaccatcg tggaacagta cgaacgcgcc gagggccgcc actccaccgg cggcatggac
     7801 gagctgtaca agtaagttta aacccgctga tcagcctcga ctgtgccttc tagttgccag
     7861 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact
     7921 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt
     7981 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat
     8041 gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg
     8101 gggtatcccc attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     8161 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     8221 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     8281 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     8341 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     8401 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     8461 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     8521 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     8581 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     8641 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     8701 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     8761 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     8821 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     8881 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     8941 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     9001 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     9061 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     9121 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     9181 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     9241 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     9301 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     9361 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     9421 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     9481 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     9541 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     9601 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     9661 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     9721 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     9781 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     9841 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     9901 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     9961 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
    10021 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
    10081 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
    10141 gccacctgac gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca
    10201 atctgctctg atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc
    10261 gctgagtagt gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc
    10321 atgaagaatc tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat
    10381 a
//
