LOCUS       sequence-217567-        4953 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pZE2-hrtR-RBS3-chuA - LuxCDABE transcriptional unit deletion of 
            plasmid pMM643, ColE1 origin, KanR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4953)
  AUTHORS   Mimee M, Nadeau P, Hayward A, Carim S, Flanagan S, Jerger L, Collins
            J, McDonnell S, Swartwout R, Citorik RJ, Bulovic V, Langer R, 
            Traverso G, Chandrakasan AP, Lu TK
  TITLE     An ingestible bacterial-electronic system to monitor 
            gastrointestinal health.
  JOURNAL   Science. 2018 May 25;360(6391):915-918. doi: 
            10.1126/science.aas9315.
  PUBMED    29798884
REFERENCE   2  (bases 1 to 4953)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2018-05-25- 25"; volume: "360"; 
            issue: "6391"; pages: "915-918"
COMMENT     Sequence Label: pMM1162
FEATURES             Location/Qualifiers
     source          1..4953
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      785..871
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      complement(3136..3724)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3216..3235)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(3812..3906)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(3937..4731)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4048..4067)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     4658..4677
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4910..4928
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cacagctaac accacgtcgt ccctatctgc tgccctaggt ctatgagtgg ttgctggata
       61 actttacggg catgcataag gctcgtataa tatattcagg gagaccacaa cggtttccct
      121 ctacaaataa ttttgtttaa ctttgaattc tatactctaa ttaatcacat aataaggacg
      181 aatttatgcc aaaatcaacc tattttagtc tttctgacga aaaacgaaaa cgtgtctatg
      241 atgcctgttt actagaattt caaacgcact ctttccatga agctaaaatc atgcacatcg
      301 taaaagcact tgatatccca agaggaagtt tttatcaata ctttgaagat ttgaaggatt
      361 catactatta tatcttgtca caggaaactg tcgagattca tgatttattt tttaatttac
      421 taaaagaata tcctctagaa gttgctctta ataaatacaa gtatcttctt cttgaaaatt
      481 tagtaaattc gccccaatat aatctttata aatatcgatt tttagattgg acttatgaat
      541 tagaaagaga ttggaagcct aaaggcgagg taactgttcc cgctcgtgaa cttgataatc
      601 ctatttccca agtattaaaa tcagtcattc acaatctagt ttatcgcatg tttagtgaaa
      661 attgggatga acaaaagttt attgaaactt acgataaaga aatcaaattg ctcacagagg
      721 gcttgcttaa ttatgttact gaaagcaaaa aatagggatc ccatggtacg cgtgctagag
      781 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt
      841 gtcggtgaac gctctcctga gtaggacaaa tccgccgccc tagaggacca aaacgaaaaa
      901 aggcccccct ttcgggaggc ctcttttctg aaatttggta ccgagttacc attgataact
      961 cacgaaaatt tttccgttac gaccatcctg tgggatgcct tgcggcgacc agtactcttt
     1021 gtcgaaagcg ttacccaaca ccaaagtagt ggtcatacct ttgagcgcct gttgtccttg
     1081 ataactgacg tagaaatcat tcacgccata gcctggttgt ttgctgtaac tgctgctgat
     1141 atgtgttgag cgatcggcaa acgtaccaac ccacccaaca gagaagccac tgtgagcgat
     1201 cggaatattc agagtgctgg taacagtatc cgggttaatg ctggagatgt attcgccggt
     1261 atcggtgtct ttgccgcggg tacggttata ggccacatca aggctaaaca gatcagtggt
     1321 atatttcgtc atcacatccc agccccagat tttggcgttc gggacgttat acgacatagt
     1381 cgtcgccgcc gcgaaatcga cggtcgtgga gatgtaatcc ttcgctttgg tatcaaagta
     1441 gctggcttta aattccagag catcattgga caacatcagg tcatcaaaac gcagcccaaa
     1501 accgtactcc tgagtttcgt tagtttccgg acgtaagttc gggtttggca cccaatagtt
     1561 ggtatagaag cgaccaatcg agaagtgctt agaatcgtta tacatttcgc ccatcgtcgg
     1621 ggcgcggaat gcctgggcat atgagccaaa taacatcagc cagttagtcg gattgatagt
     1681 catccccgca cgagatgacc atttgtcggc atcaacgtct ttgtaaccgt cactgctacc
     1741 gcgataactg tcatagcggg ttccgccaag cagggtaatc ggcagatcgc gtaaggtgat
     1801 ctcatcctgt agccagccgg agctaaaatc gatttttgct tgcgggaagc ccgtcgtcgc
     1861 gccgcccgga tgttgttcct gacgataata ctcaccgcca tatgtcagta agtgagaagc
     1921 gaaactgtcg gcaaagagag tggaacggtt ctccagcctg gctccttttg ttatctgttc
     1981 gcgatactcg ccggaactcc ccgtgttttg cgcattaata cggacttccg accaataaat
     2041 ttttgcatct gcatttaacc agtcgttgcc ctgcggggcg agtttataag aaagctgcgc
     2101 atcgcgttga attgttgaac gatcaaccat cgggttgctg ctttcagaag ccccaacggt
     2161 ctgcggattt tttggttcac gcgcgtcgtt gttgtagtaa cgcactaaac cgctcagaga
     2221 ctgggctgaa tcaatttgcc aggtcccttt cgccagcatg ttattaatgg actcgtcatt
     2281 cggcgcggtt tcaccattgc tctggcgtaa atcaccccga tcgcgactgg accaggccac
     2341 aataccatcc agattttcag ttcgcccaaa cgcgctcgcg cctaatccca ggctatggtc
     2401 ccccgtgccg ccagtaccaa agacacgaaa accactgctt tgtccttcct gcaataaatc
     2461 ttttgcatcg accgtatcgt aggagatcac tccacccagc gcgccactgc catacagtaa
     2521 tgctgaaggt ccacgaacaa tctcaacacg cttgatcagc gccggatcga gaaaagtgcc
     2581 attcaggtgt ccggtatccg ttccctgacg aacaccatcg acaagaacca gcacgccgcg
     2641 atgatcatag ccacgcatat ttacatcctg accgttggtt cgtccggtac catccagagt
     2701 aattccagga acatgacgca gcagatcggt ggctgaagta gccgtttgat tttcaggagc
     2761 ggaagtgtcg atgacgctga ccatcatagg cgcttcgaag gaactacggg cattccccgt
     2821 tgccgtaacg gtcatggttt cagtagcaaa agcaaacgtt ggcaaggtgg cagaaacagc
     2881 taaggccaat aaactcaaac gcaacgaggt aaattgcgga cgtgacatga tttaaagctc
     2941 ccttgggtta gtgttggaaa tgtgctagcc gtaaacctag gtagacctag gcgttcggct
     3001 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     3061 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     3121 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     3181 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     3241 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     3301 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     3361 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     3421 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     3481 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     3541 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
     3601 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     3661 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaagggatc
     3721 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     3781 ttaagggatt ttggtcatga ctagtgcttg gattctcacc aataaaaaac gcccggcggc
     3841 aaccgagcgt tctgaacaaa tccagatgga gttctgaggt cattactgga tctatcaaca
     3901 ggagtccaag cgagctctcg aaccccagag tcccgctcag aagaactcgt caagaaggcg
     3961 atagaaggcg atgcgctgcg aatcgggagc ggcgataccg taaagcacga ggaagcggtc
     4021 agcccattcg ccgccaagct cttcagcaat atcacgggta gccaacgcta tgtcctgata
     4081 gcggtccgcc acacccagcc ggccacagtc gatgaatcca gaaaagcggc cattttccac
     4141 catgatattc ggcaagcagg catcgccatg ggtcacgacg agatcctcgc cgtcgggcat
     4201 gcgcgccttg agcctggcga acagttcggc tggcgcgagc ccctgatgct cttcgtccag
     4261 atcatcctga tcgacaagac cggcttccat ccgagtacgt gctcgctcga tgcgatgttt
     4321 cgcttggtgg tcgaatgggc aggtagccgg atcaagcgta tgcagccgcc gcattgcatc
     4381 agccatgatg gatactttct cggcaggagc aaggtgagat gacaggagat cctgccccgg
     4441 cacttcgccc aatagcagcc agtcccttcc cgcttcagtg acaacgtcga gcacagctgc
     4501 gcaaggaacg cccgtcgtgg ccagccacga tagccgcgct gcctcgtcct gcagttcatt
     4561 cagggcaccg gacaggtcgg tcttgacaaa aagaaccggg cgcccctgcg ctgacagccg
     4621 gaacacggcg gcatcagagc agccgattgt ctgttgtgcc cagtcatagc cgaatagcct
     4681 ctccacccaa gcggccggag aacctgcgtg caatccatct tgttcaatca tgcgaaacga
     4741 tcctcatcct gtctcttgat cagatcttga tcccctgcgc catcagatcc ttggcggcaa
     4801 gaaagccatc cagtttactt tgcagggctt cccaacctta ccagagggcg ccccagctgg
     4861 caattccgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
     4921 cacgaggccc tttcgtcttc acctcgagat gct
//
