LOCUS       sequence-217570-       10817 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Targeted DNA methylation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10817)
  AUTHORS   Lu Z, Liu Z, Mao W, Wang X, Zheng X, Chen S, Cao B, Huang S, Zhang 
            X, Zhou T, Zhang Y, Huang X, Sun Q, Li JD
  TITLE     Locus-specific DNA methylation of Mecp2 promoter leads to 
            autism-like phenotypes in mice.
  JOURNAL   Cell Death Dis. 2020 Feb 3;11(2):85. doi: 10.1038/s41419-020-2290-x.
  PUBMED    32015323
REFERENCE   2  (bases 1 to 10817)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Death 
            Dis."; date: "2020-02-3"; volume: "11(2):85. doi"; pages: " 
            10.1038/s41419-020-2290-x"
COMMENT     Sequence Label: pSt1374-N-NLS-DNMT3L-L-DNMT3Amut-L-dcas9-NLS
FEATURES             Location/Qualifiers
     source          1..10817
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     629..648
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             676..696
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1351..1368
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             6385..6405
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6448..6489
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             6499..6516
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6539..6556)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6545..6769
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6815..7243
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6902..6921)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7112..7133
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7252..7272)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7257..7586
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7437..7572
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7499..7518
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7634..7681
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7700..8098
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    8256..8377
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8293..8312)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8347..8366
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8426..8442)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8426..8442)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8439..8461)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8450..8466
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8474..8480,8481..8498,8499..8504))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8519..8540
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8657..8674)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8828..9416)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8908..8927)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9587..10378,10379..10447))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10210..10229
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10448..10552)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10627..10646)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctg
      661 gctagcacca tgggacctaa gaaaaagagg aaggtggcgg ccgctggcgg cagcatgttc
      721 gaaaccgtgc ctgtgtggag gagacagcca gtccgggtgc tgtccctttt tgaagacatc
      781 aagaaagagc tgacgagttt gggctttttg gaaagtggtt ctgacccggg acaactgaag
      841 catgtggttg atgtcacaga cacagtgagg aaggatgtgg aggagtgggg acccttcgat
      901 cttgtgtacg gcgccacacc tcccctgggc cacacctgtg accgtcctcc cagctggtac
      961 ctgttccagt tccaccggct cctgcagtac gcacggccca agccaggcag ccccaggccc
     1021 ttcttctgga tgttcgtgga caatctggtg ctgaacaagg aagacctgga cgtcgcatct
     1081 cgcttcctgg agatggagcc agtcaccatc ccagatgtcc acggcggatc cttgcagaat
     1141 gctgtccgcg tgtggagcaa catcccagcc ataaggagca gcaggcactg ggctctggtt
     1201 tcggaagaag aattgtccct gctggcccag aacaagcaga gctcgaagct cgcggccaag
     1261 tggcccacca agctggtgaa gaactgcttt ctccccctaa gagaatattt caagtatttt
     1321 tcaagcagcg ggcgcagctt cagcagcgga ctagtgcccc gcggcagcca cccagctgag
     1381 aagaggaagc ccatccgggt gctgtctctc tttgatggaa tcgctacagg gctcctggtg
     1441 ctgaaggact tgggcattca ggtggaccgc tacattgcct cggaggtgtg tgaggactcc
     1501 atcacggtgg gcatggtgcg gcaccagggg aagatcatgt acgtcgggga cgtccgcagc
     1561 gtcacacaga agcatatcca ggagtggggc ccattcgatc tggtgattgg gggcagtccc
     1621 tccaatgacc tctccatcgt caaccctgct cgcaagggcc tctacgaggg cactggccgg
     1681 ctcttctttg agttctaccg cctcctgcat gatgcgcggc ccaaggaggg agatgatcgc
     1741 cccttcttct ggctctttga gaatgtggtg gccatgggcg ttagtgacaa gagggacatc
     1801 tcgcgatttc tcgagtccaa ccctgtgatg attgatgcca aagaagtgtc agctgcacac
     1861 agggcccgct acttctgggg taaccttccc ggtatgaaca ggccgttggc atccactgtg
     1921 aatgataagc tggagctgca ggagtgtctg gagcatggca ggatagccaa gttcagcaaa
     1981 gtgaggacca ttactacgag gtcaaactcc ataaagcagg gcaaagacca gcattttcct
     2041 gtcttcatga atgagaaaga ggacatctta tggtgcactg aaatggaaag ggtatttggt
     2101 ttcccagtcc actatactga cgtctccaac atgagccgct tggcgaggca gagactgctg
     2161 ggccggtcat ggagcgtgcc agtcatccgc cacctcttcg ctccgctgaa ggagtatttt
     2221 gcgtgtgtga gcggcagcga gacccccggt accagcgaga gcgccacccc cgagagcgac
     2281 aagaaatact ctattggact ggctatcggg acaaactccg ttggctgggc cgtcataacc
     2341 gacgagtata aggtgccaag caagaaattc aaggtgctgg gtaatactga ccgccattca
     2401 atcaagaaga acctgatcgg agcactcctc ttcgactccg gtgaaaccgc tgaagctact
     2461 cggctgaagc ggaccgcaag gcggagatac acccgccgca agaatcggat atgttatctg
     2521 caagagatct ttagcaacga aatggctaag gtggacgact ccttctttca ccgcctggaa
     2581 gagagctttc tggtggagga ggataagaaa cacgagaggc accctatatt cggaaatatc
     2641 gtggatgagg tggcttacca tgaaaagtat cctacaatct accatctgag gaagaagctg
     2701 gtggacagca ccgataaagc agacctgagg ctcatctatc tggccctggc tcatatgata
     2761 aagtttagag gacactttct gatcgagggc gacctgaatc ccgataattc cgatgtggat
     2821 aaactcttca ttcaactggt gcagacatat aaccaactgt tcgaggagaa tcccataaac
     2881 gcttctggtg tggatgccaa ggctattctg tccgctcggc tgtccaagtc acgcagactg
     2941 gagaatctga ttgcccaact gccaggagaa aagaagaacg gcctgtttgg gaacctcatc
     3001 gccctgagcc tgggcctgac acctaacttc aagtccaatt ttgatctggc cgaagatgct
     3061 aaactccagc tctccaagga cacctatgac gatgatctgg acaacctgct cgcacagata
     3121 ggcgaccagt acgccgatct ctttctggct gctaagaatc tctccgacgc cattctgctg
     3181 agcgacatac tccgggtcaa cactgagatc accaaagcac ctctgagcgc ctccatgata
     3241 aaacgctatg atgaacacca tcaagacctg actctgctca aagccctcgt gaggcaacag
     3301 ctgccagaga agtacaaaga gatattcttc gaccagagca agaatggata tgccggatac
     3361 atcgatggcg gagcatcaca ggaagaattt tacaagttca tcaaaccaat cctcgagaag
     3421 atggacggta ctgaagagct gctggtgaag ctgaacaggg aggacctgct gaggaagcag
     3481 aggacctttg ataatggctc cattccacat cagatacacc tgggagagct gcatgcaatc
     3541 ctccgcaggc aggaggattt ctatcctttc ctgaaggata accgggagaa gatagagaag
     3601 atcctgacct tcaggatccc ttattacgtc ggccctctgg ctagaggcaa ctcccgcttc
     3661 gcttggatga ccaggaaatc tgaggagaca attactcctt ggaacttcga agaggtcgtg
     3721 gataagggcg caagcgccca gtcattcatc gaacggatga ccaatttcga taagaacctg
     3781 ccaaacgaga aggtcctgcc caaacattca ctcctgtacg agtatttcac cgtctataac
     3841 gagctgacta aagtgaagta cgtgaccgag ggcatgagga agcctgcctt cctgtccgga
     3901 gagcagaaga aggctatcgt tgatctgctc ttcaagacta atagaaaggt gacagtgaag
     3961 cagctcaagg aggattactt taagaagatc gaatgctttg actcagtgga aatctctggc
     4021 gtggaggacc gctttaatgc cagcctgggc acttaccatg atctgctgaa gataatcaaa
     4081 gacaaagatt tcctcgataa tgaggagaac gaggacatcc tggaagatat cgtgctgacc
     4141 ctgactctgt tcgaggatag agagatgatc gaagagcgcc tgaagaccta tgcccatctg
     4201 tttgacgata aagtcatgaa acagctcaag cggcggcgct acactgggtg gggtagactc
     4261 tccaggaaac tcataaacgg catccgcgac aaacagagcg gaaagaccat cctggatttc
     4321 ctgaaatccg acggattcgc taacaggaac ttcatgcaac tgattcacga tgactctctg
     4381 acatttaaag aggacatcca gaaggcacag gtgagcggtc aaggcgacag cctgcacgag
     4441 cacatcgcca acctcgctgg atcacccgcc ataaagaagg gaatactgca gacagtcaag
     4501 gtcgtggacg aactcgtcaa agtgatgggt cggcacaagc cagagaatat cgttatcgaa
     4561 atggcaaggg agaaccaaac cacccagaag ggccagaaga actctcggga acggatgaaa
     4621 agaatcgaag agggaattaa ggagctggga tctcagatac tgaaggagca ccctgtggag
     4681 aatacacagc tccagaacga gaaactctac ctgtactacc tccagaacgg gcgggacatg
     4741 tacgttgacc aggaactcga catcaaccgg ctgtccgatt atgacgtgga cgctattgtt
     4801 ccacagtcct tcctcaaaga tgactccatt gacaacaagg tgctgaccag atccgataag
     4861 gcccgcggta agtctgacaa tgttccatca gaagaggtgg tcaagaagat gaagaattac
     4921 tggcggcagc tcctcaacgc caaactgatc acccagcgga agtttgacaa tctgactaag
     4981 gcagaaagag gaggtctgag cgaactcgac aaggccggct ttattaagag gcaactggtc
     5041 gaaacacgcc agattaccaa acacgtggca caaatcctcg actctaggat gaacactaag
     5101 tacgatgaga acgataagct gatcagggaa gtgaaagtga taactctgaa gagcaagctg
     5161 gtgtctgact tccggaagga ctttcaattc tacaaagttc gcgaaataaa caattaccat
     5221 catgctcacg atgcctatct caatgctgtc gttggcaccg ccctgatcaa gaaataccct
     5281 aaactggagt ctgagttcgt gtacggtgac tataaagtct acgatgtgag gaagatgata
     5341 gcaaagtctg agcaagagat tggcaaagcc accgccaagt acttcttcta ctctaatatc
     5401 atgaatttct ttaagactga gataaccctg gctaacggcg aaatccggaa gcgcccactg
     5461 atcgaaacaa acggagaaac aggagaaatc gtgtgggata aaggcaggga cttcgcaact
     5521 gtgcggaagg tgctgtccat gccacaagtc aatatcgtga agaagaccga agtgcagacc
     5581 ggcggattct caaaggagag catcctgcca aagcggaact ctgacaagct gatcgccagg
     5641 aagaaagatt gggacccaaa gaagtatggc ggtttcgatt cccctacagt ggcttattcc
     5701 gttctggtcg tggcaaaagt ggagaaaggc aagtccaaga aactcaagtc tgttaaggag
     5761 ctgctcggaa ttactattat ggagagatcc agcttcgaga agaatccaat cgatttcctg
     5821 gaagctaagg gctataaaga agtgaagaaa gatctcatca tcaaactgcc caagtactct
     5881 ctctttgagc tggagaatgg taggaagcgg atgctggcct ccgccggaga gctgcagaaa
     5941 ggaaacgagc tggctctgcc ctccaaatac gtgaacttcc tgtatctggc ctcccactac
     6001 gagaaactca aaggtagccc tgaagacaat gagcagaagc aactctttgt tgagcaacat
     6061 aaacactacc tggacgaaat cattgaacag attagcgagt tcagcaagcg ggttattctg
     6121 gccgatgcaa acctcgataa agtgctgagc gcatataata agcacaggga caagccaatt
     6181 cgcgaacaag cagagaatat tatccacctc tttactctga ctaatctggg cgctcctgct
     6241 gccttcaagt atttcgatac aactattgac aggaagcggt acacctctac caaagaagtt
     6301 ctcgatgcca ccctgataca ccagtcaatt accggactgt acgagactcg catcgacctg
     6361 tctcagctcg gcggcgacgg ttctcccaag aagaagagga aagtctcgag cggtggagct
     6421 gcaggatagg aattcgggcc cttcgaaggt aagcctatcc ctaaccctct cctcggtctc
     6481 gattctacgc gtaccggtca tcatcaccat caccattgag tttaaacccg ctgatcagcc
     6541 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg
     6601 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat
     6661 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag
     6721 gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg
     6781 gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc
     6841 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     6901 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     6961 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     7021 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     7081 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     7141 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     7201 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt
     7261 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     7321 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
     7381 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
     7441 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     7501 tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct
     7561 tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat
     7621 ctgatcagca cgtgttgaca attaatcatc ggcatagtat atcggcatag tataatacga
     7681 caaggtgagg aactaaacca tggccaagcc tttgtctcaa gaagaatcca ccctcattga
     7741 aagagcaacg gctacaatca acagcatccc catctctgaa gactacagcg tcgccagcgc
     7801 agctctctct agcgacggcc gcatcttcac tggtgtcaat gtatatcatt ttactggggg
     7861 accttgtgca gaactcgtgg tgctgggcac tgctgctgct gcggcagctg gcaacctgac
     7921 ttgtatcgtc gcgatcggaa atgagaacag gggcatcttg agcccctgcg gacggtgccg
     7981 acaggtgctt ctcgatctgc atcctgggat caaagccata gtgaaggaca gtgatggaca
     8041 gccgacggca gttgggattc gtgaattgct gccctctggt tatgtgtggg agggctaagc
     8101 acttcgtggc cgaggagcag gactgacacg tgctacgaga tttcgattcc accgccgcct
     8161 tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc
     8221 gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg
     8281 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     8341 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct
     8401 ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     8461 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     8521 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     8581 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     8641 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     8701 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     8761 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     8821 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     8881 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     8941 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     9001 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     9061 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     9121 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     9181 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     9241 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     9301 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     9361 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     9421 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     9481 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     9541 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     9601 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     9661 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     9721 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     9781 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     9841 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     9901 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     9961 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
    10021 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
    10081 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
    10141 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
    10201 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
    10261 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
    10321 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
    10381 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
    10441 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
    10501 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
    10561 cccgaaaagt gccacctgac gtcgacggat cgggagatct cccgatcccc tatggtgcac
    10621 tctcagtaca atctgctctg atgccgcata gttaagccag tatctgctcc ctgcttgtgt
    10681 gttggaggtc gctgagtagt gcgcgagcaa aatttaagct acaacaaggc aaggcttgac
    10741 cgacaattgc atgaagaatc tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg
    10801 ggccagatat acgcgtt
//
