LOCUS       sequence-217573-       12266 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pZE2-PLhrtO-lux-hrtR-RBS2-chuA - Composite plasmid of pMM534 and 
            pMM549, ColE1 origin, KanR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12266)
  AUTHORS   Mimee M, Nadeau P, Hayward A, Carim S, Flanagan S, Jerger L, Collins
            J, McDonnell S, Swartwout R, Citorik RJ, Bulovic V, Langer R, 
            Traverso G, Chandrakasan AP, Lu TK
  TITLE     An ingestible bacterial-electronic system to monitor 
            gastrointestinal health.
  JOURNAL   Science. 2018 May 25;360(6391):915-918. doi: 
            10.1126/science.aas9315.
  PUBMED    29798884
REFERENCE   2  (bases 1 to 12266)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2018-05-25- 25"; volume: "360"; 
            issue: "6391"; pages: "915-918"
COMMENT     Sequence Label: pMM627
FEATURES             Location/Qualifiers
     source          1..12266
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(1..35)
                     /label=BBa_J23107
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.36"
     rep_origin      complement(196..784)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(276..295)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(872..966)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(997..1791)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1108..1127)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     1718..1737
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1970..1988
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     RBS             2091..2102
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             2109..3551
                     /codon_start=1
                     /gene="luxC"
                     /product="LuxC fatty acid reductase"
                     /label=LuxC
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTKKISFIINGQVEIFPEGDDLVQSINFGDNSVYLPILNDSHVKN
                     IIDCNGNNELRLHNIVNFLYTVGQRWKNEEYSRRRTYIRDLKKYMGYSEEMAKLEANWI
                     SMILCSKGGLYDVVENELGSRHIMDEWLPQDESYVRAFPKGKSVHLLAGNVPLSGIMSI
                     LRAILTKNQCIIKTSSTDPFTANALALSFIDVDPNHPITRSLSVIYWPHQGDTSLAKEI
                     MQHADVIVAWGGPDAINWAVEHAPSYADVIKFGSKKSLCIIDNPVDLTSAATGAAHDVC
                     FYDQRACFSAQNIYYMGNHYEEFKLALIEKLNLYAHILPNAKKDFDEKAAYSLVQKESL
                     FAGLKVEVDIHQRWMIIESNAGVEFNQPLGRCVYLHHVDNIEQILPYVQKNKTQTISIF
                     PWESSFKYRDALALKGAERIVEAGMNNIFRVGGSHDGMRPLQRLVTYISHERPSNYTAK
                     DVAVEIEQTRFLEEDKFLVFVP"
     CDS             3564..4487
                     /codon_start=1
                     /gene="luxD"
                     /product="LuxD acyltransferase"
                     /label=LuxD
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MENESKYKTIDHVICVEGNKKIHVWETLPEENSPKRKNAIIIASG
                     FARRMDHFAGLAEYLSRNGFHVIRYDSLHHVGLSSGTIDEFTMSIGKQSLLAVVDWLTT
                     RKINNFGMLASSLSARIAYASLSEINASFLITAVGVVNLRYSLERALGFDYLSLPINEL
                     PNNLDFEGHKLGAEVFARDCLDFGWEDLASTINNMMYLDIPFIAFTANNDNWVKQDEVI
                     TLLSNIRSNRCKIYSLLGSSHDLSENLVVLRNFYQSVTKAAIAMDNDHLDIDVDITEPS
                     FEHLTIATVNERRMRIEIENQAISLS"
     CDS             4536..5618
                     /codon_start=1
                     /gene="luxA"
                     /product="LuxA luciferase subunit"
                     /label=LuxA
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGNFLLTYQPPQFSQTEVMKRLVKLGRISEECGFDTVWLLEHH
                     FTEFGLLGNPYVAAAYLLGATKKLNVGTAAIVLPTAHPVRQLEDVNLLDQMSKGRFRFG
                     ICRGLYNKDFRVFGTDMNNSRALAECWYGLIKNGMTEGYMEADNEHIKFHKVKVNPAAY
                     SRGGAPVYVVAESASTTEWAAQFGLPMILSWIINTNEKKAQLELYNEVAQEYGHDIHNI
                     DHCLSYITSVDHDSIKAKEICRKFLGHWYDSYVNATTIFDDSDQTRGYDFNKGQWRDFV
                     LKGHKDTNRRIDYSYEINPVGTPQECIDIIQKDIDATGISNICCGFEANGTVDEIIASM
                     KLFQSDVMPFLKEKQRSLLY"
     CDS             5633..6616
                     /codon_start=1
                     /gene="luxB"
                     /product="LuxB luciferase subunit"
                     /label=LuxB
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGLFFLNFINSTTVQEQSIVRMQEITEYVDKLNFEQILVYENH
                     FSDNGVVGAPLTVSGFLLGLTEKIKIGSLNHIITTHHPVRIAEEACLLDQLSEGRFILG
                     FSDCEKKDEMHFFNRPVEYQQQLFEECYEIINDALTTGYCNPDNDFYSFPKISVNPHAY
                     TPGGPRKYVTATSHHIVEWAAKKGIPLIFKWDDSNDVRYEYAERYKAVADKYDVDLSEI
                     DHQLMILVNYNEDSNKAKQETRAFISDYVLEMHPNENFENKLEEIIAENAVGNYTECIT
                     AAKLAIEKCGAKSVLLSFEPMNDLMSQKNVINIVDDNIKKYHMEYT"
     CDS             6795..7907
                     /codon_start=1
                     /gene="luxE"
                     /product="LuxE"
                     /label=LuxE
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTSYVDKQEITASSEIDDLIFSSDPLVWSYDEQEKIRKKLVLDAF
                     RNHYKHCREYRHYCQAHKVDDNITEIDDIPVFPTSVFKFTRLLTSQENEIESWFTSSGT
                     NGLKSQVARDRLSIERLLGSVSYGMKYVGSWFDHQIELVNLGPDRFNAHNIWFKYVMSL
                     VELLYPTTFTVTEERIDFVKTLNSLERIKNQGKDLCLIGSPYFIYLLCHYMKDKKISFS
                     GDKSLYIITGGGWKSYEKESLKRDDFNHLLFDTFNLSDISQIRDIFNQVELNTCFFEDE
                     MQRKHVPPWVYARALDPETLKPVPDGTPGLMSYMDASATSYPAFIVTDDVGIISREYGK
                     YPGVLVEILRRVNTRTQKGCALSLTEAFDS"
     RBS             8138..8149
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      8750..8836
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gctagcataa tacctagggc tgagctagcc gtaaacctag gtagacctag gcgttcggct
       61 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
      121 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
      181 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
      241 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
      301 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
      361 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
      421 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
      481 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
      541 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
      601 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
      661 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
      721 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
      781 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
      841 ttaagggatt ttggtcatga ctagtgcttg gattctcacc aataaaaaac gcccggcggc
      901 aaccgagcgt tctgaacaaa tccagatgga gttctgaggt cattactgga tctatcaaca
      961 ggagtccaag cgagctctcg aaccccagag tcccgctcag aagaactcgt caagaaggcg
     1021 atagaaggcg atgcgctgcg aatcgggagc ggcgataccg taaagcacga ggaagcggtc
     1081 agcccattcg ccgccaagct cttcagcaat atcacgggta gccaacgcta tgtcctgata
     1141 gcggtccgcc acacccagcc ggccacagtc gatgaatcca gaaaagcggc cattttccac
     1201 catgatattc ggcaagcagg catcgccatg ggtcacgacg agatcctcgc cgtcgggcat
     1261 gcgcgccttg agcctggcga acagttcggc tggcgcgagc ccctgatgct cttcgtccag
     1321 atcatcctga tcgacaagac cggcttccat ccgagtacgt gctcgctcga tgcgatgttt
     1381 cgcttggtgg tcgaatgggc aggtagccgg atcaagcgta tgcagccgcc gcattgcatc
     1441 agccatgatg gatactttct cggcaggagc aaggtgagat gacaggagat cctgccccgg
     1501 cacttcgccc aatagcagcc agtcccttcc cgcttcagtg acaacgtcga gcacagctgc
     1561 gcaaggaacg cccgtcgtgg ccagccacga tagccgcgct gcctcgtcct gcagttcatt
     1621 cagggcaccg gacaggtcgg tcttgacaaa aagaaccggg cgcccctgcg ctgacagccg
     1681 gaacacggcg gcatcagagc agccgattgt ctgttgtgcc cagtcatagc cgaatagcct
     1741 ctccacccaa gcggccggag aacctgcgtg caatccatct tgttcaatca tgcgaaacga
     1801 tcctcatcct gtctcttgat cagatcttga tcccctgcgc catcagatcc ttggcggcaa
     1861 gaaagccatc cagtttactt tgcagggctt cccaacctta ccagagggcg ccccagctgg
     1921 caattccgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
     1981 cacgaggccc tttcgtcttc acctcgagat gctataaatg acacagtgtc atttgacaaa
     2041 atgacacagt gtcatgatac tgagcacatc agcaggacgc actgaccatt aaagaggaga
     2101 aaggtaccat gactaaaaaa atttcattca ttattaacgg ccaggttgaa atctttcccg
     2161 aaggtgatga tttagtgcaa tccattaatt ttggtgataa tagtgtttac ctgccaatat
     2221 tgaatgactc tcatgtaaaa aacattattg attgtaatgg aaataacgaa ttacggttgc
     2281 ataacattgt caattttctc tatacggtag ggcaaagatg gaaaaatgaa gaatactcaa
     2341 gacgcaggac atacattcgt gacttaaaaa aatatatggg atattcagaa gaaatggcta
     2401 agctagaggc caattggata tctatgattt tatgttctaa aggcggcctt tatgatgttg
     2461 tagaaaatga acttggttct cgccatatca tggatgaatg gctacctcag gatgaaagtt
     2521 atgttcgggc ttttccgaaa ggtaaatctg tacatctgtt ggcaggtaat gttccattat
     2581 ctgggatcat gtctatatta cgcgcaattt taactaagaa tcagtgtatt ataaaaacat
     2641 cgtcaaccga tccttttacc gctaatgcat tagcgttaag ttttattgat gtagacccta
     2701 atcatccgat aacgcgctct ttatctgtta tatattggcc ccaccaaggt gatacatcac
     2761 tcgcaaaaga aattatgcaa catgcggatg ttattgtcgc ttggggaggg ccagatgcga
     2821 ttaattgggc ggtagagcat gcgccatctt atgctgatgt gattaaattt ggttctaaaa
     2881 agagtctttg cattatcgat aatcctgttg atttgacgtc cgcagcgaca ggtgcggctc
     2941 atgatgtttg tttttacgat cagcgagctt gtttttctgc ccaaaacata tattacatgg
     3001 gaaatcatta tgaggaattt aagttagcgt tgatagaaaa acttaatcta tatgcgcata
     3061 tattaccgaa tgccaaaaaa gattttgatg aaaaggcggc ctattcttta gttcaaaaag
     3121 aaagcttgtt tgctggatta aaagtagagg tggatattca tcaacgttgg atgattattg
     3181 agtcaaatgc aggtgtggaa tttaatcaac cacttggcag atgtgtgtac cttcatcacg
     3241 tcgataatat tgagcaaata ttgccttatg ttcaaaaaaa taagacgcaa accatatcta
     3301 tttttccttg ggagtcatca tttaaatatc gagatgcgtt agcattaaaa ggtgcggaaa
     3361 ggattgtaga agcaggaatg aataacatat ttcgagttgg tggatctcat gacggaatgc
     3421 gaccgttgca acgattagtg acatatattt ctcatgaaag gccatctaac tatacggcta
     3481 aggatgttgc ggttgaaata gaacagactc gattcctgga agaagataag ttccttgtat
     3541 ttgtcccata ataggtaaaa agtatggaaa atgaatcaaa atataaaacc atcgaccacg
     3601 ttatttgtgt tgaaggaaat aaaaaaattc atgtttggga aacgctgcca gaagaaaaca
     3661 gcccaaagag aaagaatgcc attattattg cgtctggttt tgcccgcagg atggatcatt
     3721 ttgctggtct ggcggaatat ttatcgcgga atggatttca tgtgatccgc tatgattcgc
     3781 ttcaccacgt tggattgagt tcagggacaa ttgatgaatt tacaatgtct ataggaaagc
     3841 agagcttgtt agcagtggtt gattggttaa ctacacgaaa aataaataac ttcggtatgt
     3901 tggcttcaag cttatctgcg cggatagctt atgcaagcct atctgaaatc aatgcttcgt
     3961 ttttaatcac cgcagtcggt gttgttaact taagatattc tcttgaaaga gctttagggt
     4021 ttgattatct cagtctaccc attaatgaat tgccgaataa tctagatttt gaaggccata
     4081 aattgggtgc tgaagtcttt gcgagagatt gtcttgattt tggttgggaa gatttagctt
     4141 ctacaattaa taacatgatg tatcttgata taccgtttat tgcttttact gcaaataacg
     4201 ataattgggt caagcaagat gaagttatca cattgttatc aaatattcgt agtaatcgat
     4261 gcaagatata ttctttgtta ggaagttcgc atgacttgag tgaaaattta gtggtcctgc
     4321 gcaattttta tcaatcggtt acgaaagccg ctatcgcgat ggataatgat catctggata
     4381 ttgatgttga tattactgaa ccgtcatttg aacatttaac tattgcgaca gtcaatgaac
     4441 gccgaatgag aattgagatt gaaaatcaag caatttctct gtcttaaaat ctattgagat
     4501 attctatcac tcaaatagca atataaggac tctctatgaa atttggaaac tttttgctta
     4561 cataccaacc tccccaattt tctcaaacag aggtaatgaa acgtttggtt aaattaggtc
     4621 gcatctctga ggagtgtggt tttgataccg tatggttact ggagcatcat ttcacggagt
     4681 ttggtttgct tggtaaccct tatgtcgctg ctgcatattt acttggcgcg actaaaaaat
     4741 tgaatgtagg aactgccgct attgttcttc ccacagccca tccagtacgc caacttgaag
     4801 atgtgaattt attggatcaa atgtcaaaag gacgatttcg gtttggtatt tgccgagggc
     4861 tttacaacaa ggactttcgc gtattcggca cagatatgaa taacagtcgc gccttagcgg
     4921 aatgctggta cgggctgata aagaatggca tgacagaggg atatatggaa gctgataatg
     4981 aacatatcaa gttccataag gtaaaagtaa accccgcggc gtatagcaga ggtggcgcac
     5041 cggtttatgt ggtggctgaa tcagcttcga cgactgagtg ggctgctcaa tttggcctac
     5101 cgatgatatt aagttggatt ataaatacta acgaaaagaa agcacaactt gagctttata
     5161 atgaagtggc tcaagaatat gggcacgata ttcataatat cgaccattgc ttatcatata
     5221 taacatctgt agatcatgac tcaattaaag cgaaagagat ttgccggaaa tttctggggc
     5281 attggtatga ttcttatgtg aatgctacga ctatttttga tgattcagac caaacaagag
     5341 gttatgattt caataaaggg cagtggcgtg actttgtatt aaaaggacat aaagatacta
     5401 atcgccgtat tgattacagt tacgaaatca atcccgtggg aacgccgcag gaatgtattg
     5461 acataattca aaaagacatt gatgctacag gaatatcaaa tatttgttgt ggatttgaag
     5521 ctaatggaac agtagacgaa attattgctt ccatgaagct cttccagtct gatgtcatgc
     5581 catttcttaa agaaaaacaa cgttcgctat tatattagct aaggagaaag aaatgaaatt
     5641 tggattgttc ttccttaact tcatcaattc aacaactgtt caagaacaaa gtatagttcg
     5701 catgcaggaa ataacggagt atgttgataa gttgaatttt gaacagattt tagtgtatga
     5761 aaatcatttt tcagataatg gtgttgtcgg cgctcctctg actgtttctg gttttctgct
     5821 cggtttaaca gagaaaatta aaattggttc attaaatcac atcattacaa ctcatcatcc
     5881 tgtccgcata gcggaggaag cttgcttatt ggatcagtta agtgaaggga gatttatttt
     5941 agggtttagt gattgcgaaa aaaaagatga aatgcatttt tttaatcgcc cggttgaata
     6001 tcaacagcaa ctatttgaag agtgttatga aatcattaac gatgctttaa caacaggcta
     6061 ttgtaatcca gataacgatt tttatagctt ccctaaaata tctgtaaatc cccatgctta
     6121 tacgccaggc ggacctcgga aatatgtaac agcaaccagt catcatattg ttgagtgggc
     6181 ggccaaaaaa ggtattcctc tcatctttaa gtgggatgat tctaatgatg ttagatatga
     6241 atatgctgaa agatataaag ccgttgcgga taaatatgac gttgacctat cagagataga
     6301 ccatcagtta atgatattag ttaactataa cgaagatagt aataaagcta aacaagagac
     6361 gcgtgcattt attagtgatt atgttcttga aatgcaccct aatgaaaatt tcgaaaataa
     6421 acttgaagaa ataattgcag aaaacgctgt cggaaattat acggagtgta taactgcggc
     6481 taagttggca attgaaaagt gtggtgcgaa aagtgtattg ctgtcctttg aaccaatgaa
     6541 tgatttgatg agccaaaaaa atgtaatcaa tattgttgat gataatatta agaagtacca
     6601 catggaatat acctaataga tttcgagttg cagcgaggcg gcaagtgaac gaatccccag
     6661 gagcatagat aactatgtga ctggggtgag tgaaagcagc caacaaagca gcagcttgaa
     6721 agatgaaggg tataaaagag tatgacagca gtgctgccat actttctaat attatcttga
     6781 ggagtaaaac aggtatgact tcatatgttg ataaacaaga aattacagca agctcagaaa
     6841 ttgatgattt gattttttcg agcgatccat tagtgtggtc ttacgacgag caggaaaaaa
     6901 tcagaaagaa acttgtgctt gatgcatttc gtaatcatta taaacattgt cgagaatatc
     6961 gtcactactg tcaggcacac aaagtagatg acaatattac ggaaattgat gacatacctg
     7021 tattcccaac atcggttttt aagtttactc gcttattaac ttctcaggaa aacgagattg
     7081 aaagttggtt taccagtagc ggcacgaatg gtttaaaaag tcaggtggcg cgtgacagat
     7141 taagtattga gagactctta ggctctgtga gttatggcat gaaatatgtt ggtagttggt
     7201 ttgatcatca aatagaatta gtcaatttgg gaccagatag atttaatgct cataatattt
     7261 ggtttaaata tgttatgagt ttggtggaat tgttatatcc tacgacattt accgtaacag
     7321 aagaacgaat agattttgtt aaaacattga atagtcttga acgaataaaa aatcaaggga
     7381 aagatctttg tcttattggt tcgccatact ttatttattt actctgccat tatatgaaag
     7441 ataaaaaaat ctcattttct ggagataaaa gcctttatat cataaccgga ggcggctgga
     7501 aaagttacga aaaagaatct ctgaaacgtg atgatttcaa tcatctttta tttgatactt
     7561 tcaatctcag tgatattagt cagatccgag atatatttaa tcaagttgaa ctcaacactt
     7621 gtttctttga ggatgaaatg cagcgtaaac atgttccgcc gtgggtatat gcgcgagcgc
     7681 ttgatcctga aacgttgaaa cctgtacctg atggaacgcc ggggttgatg agttatatgg
     7741 atgcgtcagc aaccagttat ccagcattta ttgttaccga tgatgtcggg ataattagca
     7801 gagaatatgg taagtatccc ggcgtgctcg ttgaaatttt acgtcgcgtc aatacgagga
     7861 cgcagaaagg gtgtgcttta agcttaaccg aagcgtttga tagttgaaag cttgatatcg
     7921 aattcctgcc caattattga aggggagcgg gaaaccgctc cccttttttt gtttctggtc
     7981 tccccacagc taacaccacg tcgtccctat ctgctgccct aggtctatga gtggttgctg
     8041 gataacttta cgggcatgca taaggctcgt ataatatatt cagggagacc acaacggttt
     8101 ccctctacaa ataattttgt ttaactttga attcattaaa gaggagaaag atgccaaaat
     8161 caacctattt tagtctttct gacgaaaaac gaaaacgtgt ctatgatgcc tgtttactag
     8221 aatttcaaac gcactctttc catgaagcta aaatcatgca catcgtaaaa gcacttgata
     8281 tcccaagagg aagtttttat caatactttg aagatttgaa ggattcatac tattatatct
     8341 tgtcacagga aactgtcgag attcatgatt tattttttaa tttactaaaa gaatatcctc
     8401 tagaagttgc tcttaataaa tacaagtatc ttcttcttga aaatttagta aattcgcccc
     8461 aatataatct ttataaatat cgatttttag attggactta tgaattagaa agagattgga
     8521 agcctaaagg cgaggtaact gttcccgctc gtgaacttga taatcctatt tcccaagtat
     8581 taaaatcagt cattcacaat ctagtttatc gcatgtttag tgaaaattgg gatgaacaaa
     8641 agtttattga aacttacgat aaagaaatca aattgctcac agagggcttg cttaattatg
     8701 ttactgaaag caaaaaatag ggatcccatg gtacgcgtgc tagaggcatc aaataaaacg
     8761 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct
     8821 cctgagtagg acaaatccgc cgccctagag gaccaaaacg aaaaaaggcc cccctttcgg
     8881 gaggcctctt ttctgaaatt tggtaccgag ttctgagaga tcccctcata atttccccaa
     8941 aacgtaacca tgtgtgaata gattttgagt aagcagggtt gcagccacga gtgagtcttc
     9001 ccttgttatt gtgtagccag aatgccgcaa aacttccatg cctaagcgaa ctgttgagag
     9061 tacgtttcga tttctgactg tgttagcctg gaagtgcttg tcccaacctt gtttctgagc
     9121 atgaacgccc gcaagccaac atgttagttg aagcatcagg gcgattagca gcatgatatc
     9181 aaaacgctct gagctgctcg ttcggctatg gcgtaggcct agtccgtagg caggactttt
     9241 caagtctcgg aaggtttctt caatctgcat tcgcttcgaa tagatattaa caagttgttt
     9301 gggtgttcga atttcaacag gtaagttagt tgctagaatc catggctcct ttgccgacgc
     9361 tgagtagatt ttaggtgacg ggtggtgaca atgagtccgt gtcgagcgct gattttttcg
     9421 gcctttagag cgagatttat acaatagaat ttggcatgag attgggttgc ttttagtcag
     9481 cctcttatag cctaaagtct ttgagtgact agatgacata tcatgtaagt tgctgatagg
     9541 tttccagttt tccgctccta ggtctgcata ttgtactttt cctcttactc gacttaacca
     9601 gtaccaaccc agcttctcaa cggatttata ccatggcact ttaaagccag catcactgac
     9661 aatgagcggt gtggtgttac tcggtagaat gctcgcaagg tcggctagaa attggtcatg
     9721 agctttcttt gaacattgct ctgaaagcgg gaacgctttc tcataaagag taacagaacg
     9781 accgtgtagt gcgactgaag ctcgcaatac cataagccgt ttttgctcac ggatatcaga
     9841 ccagtcaaca agtacaatgg gcatcgtatt gcccgaacag ataaagctag catgccaacg
     9901 gtatacagcg agtcgctctt tgtggaggtg acgattacct aacaatcggt cgattcgttt
     9961 gatgttatgt tttgttctcg ctttggttgg caggttacgg ccaagttcgg taagagtgag
    10021 agttttacag tcaagtaagg cgtggcaagc caacgttaag ctgttgagtc gttttaagtg
    10081 taattcgggg cagaattggt aaagagagtc gtgtaaaata tcgagttcgc acattttgtt
    10141 gtctgattat tgatttttgg cgaaaccatt tgatcatatg acaagatgtg tatctacctt
    10201 aacttaatga ttttgataaa aatcattagg ggattcatca gtaccgagtt accattgata
    10261 actcacgaaa atttttccgt tacgaccatc ctgtgggatg ccttgcggcg accagtactc
    10321 tttgtcgaaa gcgttaccca acaccaaagt agtggtcata cctttgagcg cctgttgtcc
    10381 ttgataactg acgtagaaat cattcacgcc atagcctggt tgtttgctgt aactgctgct
    10441 gatatgtgtt gagcgatcgg caaacgtacc aacccaccca acagagaagc cactgtgagc
    10501 gatcggaata ttcagagtgc tggtaacagt atccgggtta atgctggaga tgtattcgcc
    10561 ggtatcggtg tctttgccgc gggtacggtt ataggccaca tcaaggctaa acagatcagt
    10621 ggtatatttc gtcatcacat cccagcccca gattttggcg ttcgggacgt tatacgacat
    10681 agtcgtcgcc gccgcgaaat cgacggtcgt ggagatgtaa tccttcgctt tggtatcaaa
    10741 gtagctggct ttaaattcca gagcatcatt ggacaacatc aggtcatcaa aacgcagccc
    10801 aaaaccgtac tcctgagttt cgttagtttc cggacgtaag ttcgggtttg gcacccaata
    10861 gttggtatag aagcgaccaa tcgagaagtg cttagaatcg ttatacattt cgcccatcgt
    10921 cggggcgcgg aatgcctggg catatgagcc aaataacatc agccagttag tcggattgat
    10981 agtcatcccc gcacgagatg accatttgtc ggcatcaacg tctttgtaac cgtcactgct
    11041 accgcgataa ctgtcatagc gggttccgcc aagcagggta atcggcagat cgcgtaaggt
    11101 gatctcatcc tgtagccagc cggagctaaa atcgattttt gcttgcggga agcccgtcgt
    11161 cgcgccgccc ggatgttgtt cctgacgata atactcaccg ccatatgtca gtaagtgaga
    11221 agcgaaactg tcggcaaaga gagtggaacg gttctccagc ctggctcctt ttgttatctg
    11281 ttcgcgatac tcgccggaac tccccgtgtt ttgcgcatta atacggactt ccgaccaata
    11341 aatttttgca tctgcattta accagtcgtt gccctgcggg gcgagtttat aagaaagctg
    11401 cgcatcgcgt tgaattgttg aacgatcaac catcgggttg ctgctttcag aagccccaac
    11461 ggtctgcgga ttttttggtt cacgcgcgtc gttgttgtag taacgcacta aaccgctcag
    11521 agactgggct gaatcaattt gccaggtccc tttcgccagc atgttattaa tggactcgtc
    11581 attcggcgcg gtttcaccat tgctctggcg taaatcaccc cgatcgcgac tggaccaggc
    11641 cacaatacca tccagatttt cagttcgccc aaacgcgctc gcgcctaatc ccaggctatg
    11701 gtcccccgtg ccgccagtac caaagacacg aaaaccactg ctttgtcctt cctgcaataa
    11761 atcttttgca tcgaccgtat cgtaggagat cactccaccc agcgcgccac tgccatacag
    11821 taatgctgaa ggtccacgaa caatctcaac acgcttgatc agcgccggat cgagaaaagt
    11881 gccattcagg tgtccggtat ccgttccctg acgaacacca tcgacaagaa ccagcacgcc
    11941 gcgatgatca tagccacgca tatttacatc ctgaccgttg gttcgtccgg taccatccag
    12001 agtaattcca ggaacatgac gcagcagatc ggtggctgaa gtagccgttt gattttcagg
    12061 agcggaagtg tcgatgacgc tgaccatcat aggcgcttcg aaggaactac gggcattccc
    12121 cgttgccgta acggtcatgg tttcagtagc aaaagcaaac gttggcaagg tggcagaaac
    12181 agctaaggcc aataaactca aacgcaacga ggtaaattgc ggacgtgaca tgatttaaag
    12241 ctcccttggg ttagtgttgg aaatgt
//
