LOCUS       sequence-217579-        4989 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pZA1D-hrtR-chuA - HrtR expressed consitutively from promoter ProD 
            with RBS2, ChuA expressed consitiutively from promoter J23107, p15a 
            origin, AmpR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4989)
  AUTHORS   Mimee M, Nadeau P, Hayward A, Carim S, Flanagan S, Jerger L, Collins
            J, McDonnell S, Swartwout R, Citorik RJ, Bulovic V, Langer R, 
            Traverso G, Chandrakasan AP, Lu TK
  TITLE     An ingestible bacterial-electronic system to monitor 
            gastrointestinal health.
  JOURNAL   Science. 2018 May 25;360(6391):915-918. doi: 
            10.1126/science.aas9315.
  PUBMED    29798884
REFERENCE   2  (bases 1 to 4989)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2018-05-25- 25"; volume: "360"; 
            issue: "6391"; pages: "915-918"
COMMENT     Sequence Label: pMM549
FEATURES             Location/Qualifiers
     source          1..4989
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(1..35)
                     /label=BBa_J23107
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.36"
     primer_bind     complement(57..74)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(191..736)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(271..290)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(850..944)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(974..1765,1766..1834))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1597..1616
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1835..1939)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2007..2025
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     RBS             2199..2210
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      2811..2897
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gctagcataa tacctagggc tgagctagcc gtaaacctag ggatatattc cgcttcctcg
       61 ctcactgact cgctacgctc ggtcgttcga ctgcggcgag cggaaatggc ttacgaacgg
      121 ggcggagatt tcctggaaga tgccaggaag atacttaaca gggaagtgag agggccgcgg
      181 caaagccgtt tttccatagg ctccgccccc ctgacaagca tcacgaaatc tgacgctcaa
      241 atcagtggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggcggct
      301 ccctcgtgcg ctctcctgtt cctgcctttc ggtttaccgg tgtcattccg ctgttatggc
      361 cgcgtttgtc tcattccacg cctgacactc agttccgggt aggcagttcg ctccaagctg
      421 gactgtatgc acgaaccccc cgttcagtcc gaccgctgcg ccttatccgg taactatcgt
      481 cttgagtcca acccggaaag acatgcaaaa gcaccactgg cagcagccac tggtaattga
      541 tttagaggag ttagtcttga agtcatgcgc cggttaaggc taaactgaaa ggacaagttt
      601 tggtgactgc gctcctccaa gccagttacc tcggttcaaa gagttggtag ctcagagaac
      661 cttcgaaaaa ccgccctgca aggcggtttt ttcgttttca gagcaagaga ttacgcgcag
      721 accaaaacga tctcaagaag atcatcttat taatcagata aaatattact agatttcagt
      781 gcaatttatc tcttcaaatg tagcacctga agtcagcccc atacgatata agttgttact
      841 agtgcttgga ttctcaccaa taaaaaacgc ccggcggcaa ccgagcgttc tgaacaaatc
      901 cagatggagt tctgaggtca ttactggatc tatcaacagg agtccaagcg agctcgtaaa
      961 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     1021 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     1081 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     1141 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     1201 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     1261 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     1321 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     1381 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     1441 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     1501 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     1561 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     1621 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     1681 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     1741 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     1801 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     1861 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     1921 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
     1981 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtcttcacc
     2041 tcgagcacag ctaacaccac gtcgtcccta tctgctgccc taggtctatg agtggttgct
     2101 ggataacttt acgggcatgc ataaggctcg tataatatat tcagggagac cacaacggtt
     2161 tccctctaca aataattttg tttaactttg aattcattaa agaggagaaa gatgccaaaa
     2221 tcaacctatt ttagtctttc tgacgaaaaa cgaaaacgtg tctatgatgc ctgtttacta
     2281 gaatttcaaa cgcactcttt ccatgaagct aaaatcatgc acatcgtaaa agcacttgat
     2341 atcccaagag gaagttttta tcaatacttt gaagatttga aggattcata ctattatatc
     2401 ttgtcacagg aaactgtcga gattcatgat ttatttttta atttactaaa agaatatcct
     2461 ctagaagttg ctcttaataa atacaagtat cttcttcttg aaaatttagt aaattcgccc
     2521 caatataatc tttataaata tcgattttta gattggactt atgaattaga aagagattgg
     2581 aagcctaaag gcgaggtaac tgttcccgct cgtgaacttg ataatcctat ttcccaagta
     2641 ttaaaatcag tcattcacaa tctagtttat cgcatgttta gtgaaaattg ggatgaacaa
     2701 aagtttattg aaacttacga taaagaaatc aaattgctca cagagggctt gcttaattat
     2761 gttactgaaa gcaaaaaata gggatcccat ggtacgcgtg ctagaggcat caaataaaac
     2821 gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc
     2881 tcctgagtag gacaaatccg ccgccctaga ggaccaaaac gaaaaaaggc ccccctttcg
     2941 ggaggcctct tttctgaaat ttggtaccga gttaccattg ataactcacg aaaatttttc
     3001 cgttacgacc atcctgtggg atgccttgcg gcgaccagta ctctttgtcg aaagcgttac
     3061 ccaacaccaa agtagtggtc atacctttga gcgcctgttg tccttgataa ctgacgtaga
     3121 aatcattcac gccatagcct ggttgtttgc tgtaactgct gctgatatgt gttgagcgat
     3181 cggcaaacgt accaacccac ccaacagaga agccactgtg agcgatcgga atattcagag
     3241 tgctggtaac agtatccggg ttaatgctgg agatgtattc gccggtatcg gtgtctttgc
     3301 cgcgggtacg gttataggcc acatcaaggc taaacagatc agtggtatat ttcgtcatca
     3361 catcccagcc ccagattttg gcgttcggga cgttatacga catagtcgtc gccgccgcga
     3421 aatcgacggt cgtggagatg taatccttcg ctttggtatc aaagtagctg gctttaaatt
     3481 ccagagcatc attggacaac atcaggtcat caaaacgcag cccaaaaccg tactcctgag
     3541 tttcgttagt ttccggacgt aagttcgggt ttggcaccca atagttggta tagaagcgac
     3601 caatcgagaa gtgcttagaa tcgttataca tttcgcccat cgtcggggcg cggaatgcct
     3661 gggcatatga gccaaataac atcagccagt tagtcggatt gatagtcatc cccgcacgag
     3721 atgaccattt gtcggcatca acgtctttgt aaccgtcact gctaccgcga taactgtcat
     3781 agcgggttcc gccaagcagg gtaatcggca gatcgcgtaa ggtgatctca tcctgtagcc
     3841 agccggagct aaaatcgatt tttgcttgcg ggaagcccgt cgtcgcgccg cccggatgtt
     3901 gttcctgacg ataatactca ccgccatatg tcagtaagtg agaagcgaaa ctgtcggcaa
     3961 agagagtgga acggttctcc agcctggctc cttttgttat ctgttcgcga tactcgccgg
     4021 aactccccgt gttttgcgca ttaatacgga cttccgacca ataaattttt gcatctgcat
     4081 ttaaccagtc gttgccctgc ggggcgagtt tataagaaag ctgcgcatcg cgttgaattg
     4141 ttgaacgatc aaccatcggg ttgctgcttt cagaagcccc aacggtctgc ggattttttg
     4201 gttcacgcgc gtcgttgttg tagtaacgca ctaaaccgct cagagactgg gctgaatcaa
     4261 tttgccaggt ccctttcgcc agcatgttat taatggactc gtcattcggc gcggtttcac
     4321 cattgctctg gcgtaaatca ccccgatcgc gactggacca ggccacaata ccatccagat
     4381 tttcagttcg cccaaacgcg ctcgcgccta atcccaggct atggtccccc gtgccgccag
     4441 taccaaagac acgaaaacca ctgctttgtc cttcctgcaa taaatctttt gcatcgaccg
     4501 tatcgtagga gatcactcca cccagcgcgc cactgccata cagtaatgct gaaggtccac
     4561 gaacaatctc aacacgcttg atcagcgccg gatcgagaaa agtgccattc aggtgtccgg
     4621 tatccgttcc ctgacgaaca ccatcgacaa gaaccagcac gccgcgatga tcatagccac
     4681 gcatatttac atcctgaccg ttggttcgtc cggtaccatc cagagtaatt ccaggaacat
     4741 gacgcagcag atcggtggct gaagtagccg tttgattttc aggagcggaa gtgtcgatga
     4801 cgctgaccat cataggcgct tcgaaggaac tacgggcatt ccccgttgcc gtaacggtca
     4861 tggtttcagt agcaaaagca aacgttggca aggtggcaga aacagctaag gccaataaac
     4921 tcaaacgcaa cgaggtaaat tgcggacgtg acatgattta aagctccctt gggttagtgt
     4981 tggaaatgt
//
