LOCUS       sequence-217581-        5761 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5761)
  AUTHORS   Chen W, Possemato R, Campbell KT, Plattner CA, Pallas DC, Hahn WC
  TITLE     Identification of specific PP2A complexes involved in human cell 
            transformation.
  JOURNAL   Cancer Cell. 2004 Feb . 5(2):127-36.
  PUBMED    14998489
REFERENCE   2  (bases 1 to 5761)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer 
            Cell. 2004 Feb . 5(2):127-36."
COMMENT     Sequence Label: pBABE zeo PPP2CA Wt
FEATURES             Location/Qualifiers
     source          1..5761
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             22..491
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    556..755
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             956..1372
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1302..1324
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1340..1356
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(2380..2400)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2385..2714
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2565..2700
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2724..3098
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     LTR             3269..3738
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(3868..3885)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4039..4627)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4119..4138)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4787..5578,5579..5647))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5410..5429
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5648..5752)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccgaaaagtg ccacctgcag cctgaatatg ggccaaacag gatatctgtg gtaagcagtt
       61 cctgccccgg ctcagggcca agaacagatg gaacagctga atatgggcca aacaggatat
      121 ctgtggtaag cagttcctgc cccggctcag ggccaagaac agatggtccc cagatgcggt
      181 ccagccctca gcagtttcta gagaaccatc agatgtttcc agggtgcccc aaggacctga
      241 aatgaccctg tgccttattt gaactaacca atcagttcgc ttctcgcttc tgttcgcgcg
      301 cttctgctcc ccgagctcaa taaaagagcc cacaacccct cactcggggc gccagtcctc
      361 cgattgactg agtcgcccgg gtacccgtgt atccaataaa ccctcttgca gttgcatccg
      421 acttgtggtc tcgctgttcc ttgggagggt ctcctctgag tgattgacta cccgtcagcg
      481 ggggtctttc atttgggggc tcgtccggga tcgggagacc cctgcccagg gaccaccgac
      541 ccaccaccgg gaggcaagct ggccagcaac ttatctgtgt ctgtccgatt gtctagtgtc
      601 tatgactgat tttatgcgcc tgcgtcggta ctagttagct aactagctct gtatctggcg
      661 gacccgtggt ggaactgacg agttctgaac acccggccgc aaccctggga gacgtcccag
      721 ggactttggg ggccgttttt gtggcccgac ctgaggaagg gagtcgatgt ggaatccgac
      781 cccgtcagga tatgtggttc tggtaggaga cgagaaccta aaacagttcc cgcctccgtc
      841 tgaatttttg ctttcggttt ggaaccgaag ccgcgcgtct tgtctgctgc agcgctgcag
      901 catcgttctg tgttgtctct gtctgactgt gtttctgtat ttgtctgaaa attagggcca
      961 gactgttacc actcccttaa gtttgacctt aggtcactgg aaagatgtcg agcggatcgc
     1021 tcacaaccag tcggtagatg tcaagaagag acgttgggtt accttctgct ctgcagaatg
     1081 gccaaccttt aacgtcggat ggccgcgaga cggcaccttt aaccgagacc tcatcaccca
     1141 ggttaagatc aaggtctttt cacctggccc gcatggacac ccagaccagg tcccctacat
     1201 cgtgacctgg gaagccttgg cttttgaccc ccctccctgg gtcaagccct ttgtacaccc
     1261 taagcctccg cctcctcttc ctccatccgc cccgtctctc ccccttgaac ctcctcgttc
     1321 gaccccgcct cgatcctccc tttatccagc cctcactcct tctctaggcg ccggccggat
     1381 cagcttgatg gctggggcgg gtggcatcat ggacgagaag gtgttcacca aggagctgga
     1441 ccagtggatc gagcagctga acgagtgcaa gcagctgtcc gagtcccagg tcaagagcct
     1501 ctgcgagaag gctaaagaaa tcctgacaaa agaatccaac gtgcaagagg ttcgatgtcc
     1561 agttactgtc tgtggagatg tgcatgggca atttcatgat ctcatggaac tgtttagaat
     1621 tggtggcaaa tcaccagata caaattactt gtttatggga gattatgttg acagaggata
     1681 ttattcagtt gaaacagtta cactgcttgt agctcttaag gttcgttacc gtgaacgcat
     1741 caccattctt cgagggaatc atgagagcag acagatcaca caagtttatg gtttctatga
     1801 tgaatgttta agaaaatatg gaaatgcaaa tgtttggaaa tattttacag atctttttga
     1861 ctatcttcct ctcactgcct tggtggatgg gcagatcttc tgtctacatg gtggtctctc
     1921 gccatctata gatacactgg atcatatcag agcacttgat cgcctacaag aagttcccca
     1981 tgagggtcca atgtgtgact tgctgtggtc agatccagat gaccgtggtg gttggggtat
     2041 atctcctcga ggagctggtt acacctttgg gcaagatatt tctgagacat ttaatcatgc
     2101 caatggcctc acgttggtgt ctagagctca ccagctagtg atggagggat ataactggtg
     2161 ccatgaccgg aatgtagtaa cgattttcag tgctccaaac tattgttatc gttgtggtaa
     2221 ccaagctgca atcatggaac ttgacgatac tctaaaatac tctttcttgc agtttgaccc
     2281 agcacctcgt agaggcgagc cacatgttac tcgtcgtacc ccagactact tcctgtaatg
     2341 aaattttatc gaattcgcca gcacagtggt cgaccctgtg gaatgtgtgt cagttagggt
     2401 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     2461 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     2521 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
     2581 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     2641 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     2701 taggcttttg caaaaagctt accatggcca agttgaccag tgccgttccg gtgctcaccg
     2761 cgcgcgacgt cgccggagcg gtcgagttct ggaccgaccg gctcgggttc tcccgggact
     2821 tcgtggagga cgacttcgcc ggtgtggtcc gggacgacgt gaccctgttc atcagcgcgg
     2881 tccaggacca ggtggtgccg gacaacaccc tggcctgggt gtgggtgcgc ggcctggacg
     2941 agctgtacgc cgagtggtcg gaggtcgtgt ccacgaactt ccgggacgcc tccgggccgg
     3001 ccatgaccga gatcggcgag cagccgtggg ggcgggagtt cgccctgcgc gacccggccg
     3061 gcaactgcgt gcacttcgtg gccgaggagc aggactgacc gatcgataaa ataaaagatt
     3121 ttatttagtc tccagaaaaa ggggggaatg aaagacccca cctgtaggtt tggcaagcta
     3181 gcttaagtaa cgccattttg caaggcatgg aaaaatacat aactgagaat agagaagttc
     3241 agatcaaggt caggaacaga tggaacagct gaatatgggc caaacaggat atctgtggta
     3301 agcagttcct gccccggctc agggccaaga acagatggaa cagctgaata tgggccaaac
     3361 aggatatctg tggtaagcag ttcctgcccc ggctcagggc caagaacaga tggtccccag
     3421 atgcggtcca gccctcagca gtttctagag aaccatcaga tgtttccagg gtgccccaag
     3481 gacctgaaat gaccctgtgc cttatttgaa ctaaccaatc agttcgcttc tcgcttctgt
     3541 tcgcgcgctt ctgctccccg agctcaataa aagagcccac aacccctcac tcggggcgcc
     3601 agtcctccga ttgactgagt cgcccgggta cccgtgtatc caataaaccc tcttgcagtt
     3661 gcatccgact tgtggtctcg ctgttccttg ggagggtctc ctctgagtga ttgactaccc
     3721 gtcagcgggg gtctttcaca tgcagcatgt atcaaaatta atttggtttt ttttcttaag
     3781 tatttacatt aaatggccat agttgcatta atgaatcggc caacgcgcgg ggagaggcgg
     3841 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     3901 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     3961 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     4021 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     4081 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     4141 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     4201 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     4261 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     4321 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     4381 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     4441 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     4501 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     4561 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     4621 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     4681 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttgcg
     4741 gccgcaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     4801 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     4861 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     4921 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     4981 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     5041 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     5101 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     5161 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     5221 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     5281 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     5341 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     5401 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     5461 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     5521 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     5581 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     5641 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     5701 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     5761 c
//
