LOCUS       sequence-217596-       12066 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Complementation of Dicer_KO mESCs with wild type human DICER 
            isoform.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12066)
  TITLE     Ciaudo Lab Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12066)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pMSCV_puro_41584
FEATURES             Location/Qualifiers
     source          1..12066
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..500
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(36..59)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     433..452
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             521..1120
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(521..540)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     1017..1037
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             1220..1734
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    1896..1912
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1920..1926,1927..1944,1945..1950))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1965..1986
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2103..2120)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2274..2862)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2354..2373)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3033..3824,3825..3893))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3656..3675
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3894..3998)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4066..4084
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4122..4144)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4244..4263
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4457..4479
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4607..4626
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     LTR             4826..5342
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     misc_feature    5406..5747
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             5814..6230
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     6160..6182
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     6198..6214
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             6252..6275
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gggtagggga ggcgcttttc ccaaggcagt ctggagcatg cgctttagca gccccgctgg
       61 gcacttggcg ctacacaagt ggcctctggc ctcgcacaca ttccacatcc accggtaggc
      121 gccaaccggc tccgttcttt ggtggcccct tcgcgccacc ttctactcct cccctagtca
      181 ggaagttccc ccccgccccg cagctcgcgt cgtgcaggac gtgacaaatg gaagtagcac
      241 gtctcactag tctcgtgcag atggacagca ccgctgagca atggaagcgg gtaggccttt
      301 ggggcagcgg ccaatagcag ctttgctcct tcgctttctg ggctcagagg ctgggaaggg
      361 gtgggtccgg gggcgggctc aggggcgggc tcaggggcgg ggcgggcgcc cgaaggtcct
      421 ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg ttctcctctt
      481 cctcatctcc gggcctttcg acctgcagcc caagcttacc atgaccgagt acaagcccac
      541 ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt
      601 cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac
      661 cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc
      721 ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt
      781 gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca
      841 acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac
      901 cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg
      961 agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa
     1021 cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg
     1081 accgcgcacc tggtgcatga cccgcaagcc cggtgcctga cgcccgcccc acgacccgca
     1141 gcgcccgacc gaaaggagcg cacgacccca tgcatcgata aaataaaaga ttttatttag
     1201 tctccagaaa aaggggggaa tgaaagaccc cacctgtagg tttggcaagc tagcttaagt
     1261 aacgccattt tgcaaggcat ggaaaataca taactgagaa tagagaagtt cagatcaagg
     1321 ttaggaacag agagacagca gaatatgggc caaacaggat atctgtggta agcagttcct
     1381 gccccggctc agggccaaga acagatggtc cccagatgcg gtcccgccct cagcagtttc
     1441 tagagaacca tcagatgttt ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat
     1501 ttgaactaac caatcagttc gcttctcgct tctgttcgcg cgcttctgct ccccgagctc
     1561 aataaaagag cccacaaccc ctcactcggc gcgccagtcc tccgatagac tgcgtcgccc
     1621 gggtacccgt gtatccaata aaccctcttg cagttgcatc cgacttgtgg tctcgctgtt
     1681 ccttgggagg gtctcctctg agtgattgac tacccgtcag cgggggtctt tcatgggtaa
     1741 cagtttcttg aagttggaga acaacattct gagggtagga gtcgaatatt aagtaatcct
     1801 gactcaatta gccactgttt tgaatccaca tactccaata ctcctgaaat agttcattat
     1861 ggacagcgca gaaagagctg gggagaattg tgaaattgtt atccgctcac aattccacac
     1921 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     1981 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     2041 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     2101 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     2161 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     2221 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     2281 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     2341 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     2401 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     2461 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     2521 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     2581 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     2641 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     2701 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     2761 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     2821 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     2881 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     2941 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     3001 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     3061 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     3121 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     3181 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     3241 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     3301 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     3361 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     3421 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     3481 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     3541 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     3601 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     3661 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     3721 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     3781 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     3841 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     3901 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     3961 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     4021 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
     4081 aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc
     4141 ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc
     4201 gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt
     4261 gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac
     4321 cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg
     4381 gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg
     4441 gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggcgcaagg aatggtgcat
     4501 gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga
     4561 aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga
     4621 tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt
     4681 agaggcgatt agtccaattt gttaaagaca ggatatcagt ggtccaggct ctagttttga
     4741 ctcaacaata tcaccagctg aagcctatag agtacgagcc atagataaaa taaaagattt
     4801 tatttagtct ccagaaaaag gggggaatga aagaccccac ctgtaggttt ggcaagctag
     4861 cttaagtaac gccattttgc aaggcatgga aaatacataa ctgagaatag agaagttcag
     4921 atcaaggtta ggaacagaga gacagcagaa tatgggccaa acaggatatc tgtggtaagc
     4981 agttcctgcc ccggctcagg gccaagaaca gatggtcccc agatgcggtc ccgccctcag
     5041 cagtttctag agaaccatca gatgtttcca gggtgcccca aggacctgaa atgaccctgt
     5101 gccttatttg aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc
     5161 cgagctcaat aaaagagccc acaacccctc actcggcgcg ccagtcctcc gatagactgc
     5221 gtcgcccggg tacccgtatt cccaataaag cctcttgctg tttgcatccg aatcgtggac
     5281 tcgctgatcc ttgggagggt ctcctcagat tgattgactg cccacctcgg gggtctttca
     5341 tttggaggtt ccaccgagat ttggagaccc ctgcccaggg accaccgacc cccccgccgg
     5401 gaggtaagct ggccagcggt cgtttcgtgt ctgtctctgt ctttgtgcgt gtttgtgccg
     5461 gcatctaatg tttgcgcctg cgtctgtact agttagctaa ctagctctgt atctggcgga
     5521 cccgtggtgg aactgacgag ttctgaacac ccggccgcaa ccctgggaga cgtcccaggg
     5581 actttggggg ccgtttttgt ggcccgacct gaggaaggga gtcgatgtgg aatccgaccc
     5641 cgtcaggata tgtggttctg gtaggagacg agaacctaaa acagttcccg cctccgtctg
     5701 aatttttgct ttcggtttgg aaccgaagcc gcgcgtcttg tctgctgcag cgctgcagca
     5761 tcgttctgtg ttgtctctgt ctgactgtgt ttctgtattt gtctgaaaat tagggccaga
     5821 ctgttaccac tcccttaagt ttgaccttag gtcactggaa agatgtcgag cggatcgctc
     5881 acaaccagtc ggtagatgtc aagaagagac gttgggttac cttctgctct gcagaatggc
     5941 caacctttaa cgtcggatgg ccgcgagacg gcacctttaa ccgagacctc atcacccagg
     6001 ttaagatcaa ggtcttttca cctggcccgc atggacaccc agaccaggtc ccctacatcg
     6061 tgacctggga agccttggct tttgaccccc ctccctgggt caagcccttt gtacacccta
     6121 agcctccgcc tcctcttcct ccatccgccc cgtctctccc ccttgaacct cctcgttcga
     6181 ccccgcctcg atcctccctt tatccagccc tcactccttc tctaggcgcc ggaattagat
     6241 ctctcgagat ggactacaaa gacgatgacg acaagcttaa aagccctgct ttgcaacccc
     6301 tcagcatggc aggcctgcag ctcatgaccc ctgcttcctc accaatgggt cctttctttg
     6361 gactgccatg gcaacaagaa gcaattcatg ataacattta tacgccaaga aaatatcagg
     6421 ttgaactgct tgaagcagct ctggatcata ataccatcgt ctgtttaaac actggctcag
     6481 ggaagacatt tattgcagta ctactcacta aagagctgtc ctatcagatc aggggagact
     6541 tcagcagaaa tggaaaaagg acggtgttct tggtcaactc tgcaaaccag gttgctcaac
     6601 aagtgtcagc tgtcagaact cattcagatc tcaaggttgg ggaatactca aacctagaag
     6661 taaatgcatc ttggacaaaa gagagatgga accaagagtt tactaagcac caggttctca
     6721 ttatgacttg ctatgtcgcc ttgaatgttt tgaaaaatgg ttacttatca ctgtcagaca
     6781 ttaacctttt ggtgtttgat gagtgtcatc ttgcaatcct agaccacccc tatcgagaaa
     6841 ttatgaagct ctgtgaaaat tgtccatcat gtcctcgcat tttgggacta actgcttcca
     6901 ttttaaatgg gaaatgtgat ccagaggaat tggaagaaaa gattcagaaa ctagagaaaa
     6961 ttcttaagag taatgctgaa actgcaactg acctggtggt cttagacagg tatacttctc
     7021 agccatgtga gattgtggtg gattgtggac catttactga cagaagtggg ctttatgaaa
     7081 gactgctgat ggaattagaa gaagcactta attttatcaa tgattgtaat atatctgtac
     7141 attcaaaaga aagagattct actttaattt cgaaacagat actatcagac tgtcgtgccg
     7201 tattggtagt tctgggaccc tggtgtgcag ataaagtagc tggaatgatg gtaagagaac
     7261 tacagaaata catcaaacat gagcaagagg agctgcacag gaaattttta ttgtttacag
     7321 acactttcct aaggaaaata catgcactat gtgaagagca cttctcacct gcctcacttg
     7381 acctgaaatt tgtaactcct aaagtaatca aactgctcga aatcttacgc aaatataaac
     7441 catatgagcg acagcagttt gaaagcgttg agtggtataa taatagaaat caggataatt
     7501 atgtgtcatg gagtgattct gaggatgatg atgaggatga agaaattgaa gaaaaagaga
     7561 agccagagac aaattttcct tctcctttta ccaacatttt gtgcggaatt atttttgtgg
     7621 aaagaagata cacagcagtt gtcttaaaca gattgataaa ggaagctggc aaacaagatc
     7681 cagagctggc ttatatcagt agcaatttca taactggaca tggcattggg aagaatcagc
     7741 ctcgcaacaa acagatggaa gcagaattca gaaaacagga agaggtactt aggaaatttc
     7801 gagcacatga gaccaacctg cttattgcaa caagtattgt agaagagggt gttgatatac
     7861 caaaatgcaa cttggtggtt cgttttgatt tgcccacaga atatcgatcc tatgttcaat
     7921 ctaaaggaag agcaagggca cccatctcta attatataat gttagcggat acagacaaaa
     7981 taaaaagttt tgaagaagac cttaaaacct acaaagctat tgaaaagatc ttgagaaaca
     8041 agtgttccaa gtcggttgat actggtgaga ctgacattga tcctgtcatg gatgatgatg
     8101 acgttttccc accatatgtg ttgaggcctg acgatggtgg tccacgagtc acaatcaaca
     8161 cggccattgg acacatcaat agatactgtg ctagattacc aagtgatccg tttactcatc
     8221 tagctcctaa atgcagaacc cgagagttgc ctgatggtac attttattca actctttatc
     8281 tgccaattaa ctcacctctt cgagcctcca ttgttggtcc accaatgagc tgtgtacgat
     8341 tggctgaaag agttgtagct ctcatttgct gtgagaaact gcacaaaatt ggcgaactgg
     8401 atgaccattt gatgccagtt gggaaagaga ctgttaaata tgaagaggag cttgatttgc
     8461 atgatgaaga agagaccagt gttccaggaa gaccaggttc cacgaaacga aggcagtgct
     8521 acccaaaagc aattccagag tgtttgaggg atagttatcc cagacctgat cagccctgtt
     8581 acctgtatgt gataggaatg gttttaacta cacctttacc tgatgaactc aactttagaa
     8641 ggcggaagct ctatcctcct gaagatacca caagatgctt tggaatactg acggccaaac
     8701 ccatacctca gattccacac tttcctgtgt acacacgctc tggagaggtt accatatcca
     8761 ttgagttgaa gaagtctggt ttcatgttgt ctctacaaat gcttgagttg attacaagac
     8821 ttcaccagta tatattctca catattcttc ggcttgaaaa acctgcacta gaatttaaac
     8881 ctacagacgc tgattcagca tactgtgttc tacctcttaa tgttgttaat gactccagca
     8941 ctttggatat tgactttaaa ttcatggaag atattgagaa gtctgaagct cgcataggca
     9001 ttcccagtac aaagtataca aaagaaacac cctttgtttt taaattagaa gattaccaag
     9061 atgccgttat cattccaaga tatcgcaatt ttgatcagcc tcatcgattt tatgtagctg
     9121 atgtgtacac tgatcttacc ccactcagta aatttccttc ccctgagtat gaaacttttg
     9181 cagaatatta taaaacaaag tacaaccttg acctaaccaa tctcaaccag ccactgctgg
     9241 atgtggacca cacatcttca agacttaatc ttttgacacc tcgacatttg aatcagaagg
     9301 ggaaagcgct tcctttaagc agtgctgaga agaggaaagc caaatgggaa agtctgcaga
     9361 ataaacagat actggttcca gaactctgtg ctatacatcc aattccagca tcactgtgga
     9421 gaaaagctgt ttgtctcccc agcatacttt atcgccttca ctgccttttg actgcagagg
     9481 agctaagagc ccagactgcc agcgatgctg gcgtgggagt cagatcactt cctgcggatt
     9541 ttagataccc taacttagac ttcgggtgga aaaaatctat tgacagcaaa tctttcatct
     9601 caatttctaa ctcctcttca gctgaaaatg ataattactg taagcacagc acaattgtcc
     9661 ctgaaaatgc tgcacatcaa ggtgctaata gaacctcctc tctagaaaat catgaccaaa
     9721 tgtctgtgaa ctgcagaacg ttgctcagcg agtcccctgg taagctccac gttgaagttt
     9781 cagcagatct tacagcaatt aatggtcttt cttacaatca aaatctcgcc aatggcagtt
     9841 atgatttagc taacagagac ttttgccaag gaaatcagct aaattactac aagcaggaaa
     9901 tacccgtgca accaactacc tcatattcca ttcagaattt atacagttac gagaaccagc
     9961 cccagcccag cgatgaatgt actctcctga gtaataaata ccttgatgga aatgctaaca
    10021 aatctacctc agatggaagt cctgtgatgg ccgtaatgcc tggtacgaca gacactattc
    10081 aagtgctcaa gggcaggatg gattctgagc agagcccttc tattgggtac tcctcaagga
    10141 ctcttggccc caatcctgga cttattcttc aggctttgac tctgtcaaac gctagtgatg
    10201 gatttaacct ggagcggctt gaaatgcttg gcgactcctt tttaaagcat gccatcacca
    10261 catatctatt ttgcacttac cctgatgcgc atgagggccg cctttcatat atgagaagca
    10321 aaaaggtcag caactgtaat ctgtatcgcc ttggaaaaaa gaagggacta cccagccgca
    10381 tggtggtgtc aatatttgat ccccctgtga attggcttcc tcctggttat gtagtaaatc
    10441 aagacaaaag caacacagat aaatgggaaa aagatgaaat gacaaaagac tgcatgctgg
    10501 cgaatggcaa actggatgag gattacgagg aggaggatga ggaggaggag agcctgatgt
    10561 ggagggctcc gaaggaagag gctgactatg aagatgattt cctggagtat gatcaggaac
    10621 atatcagatt tatagataat atgttaatgg ggtcaggagc ttttgtaaag aaaatctctc
    10681 tttctccttt ttcaaccact gattctgcat atgaatggaa aatgcccaaa aaatcctcct
    10741 taggtagtat gccattttca tcagattttg aggattttga ctacagctct tgggatgcaa
    10801 tgtgctatct ggatcctagc aaagctgttg aagaagatga ctttgtggtg gggttctgga
    10861 atccatcaga agaaaactgt ggtgttgaca cgggaaagca gtccatttct tacgacttgc
    10921 acactgagca gtgtattgct gacaaaagca tagcggactg tgtggaagcc ctgctgggct
    10981 gctatttaac cagctgtggg gagagggctg ctcagctttt cctctgttca ctggggctga
    11041 aggtgctccc ggtaattaaa aggactgatc gggaaaaggc cctgtgccct actcgggaga
    11101 atttcaacag ccaacaaaag aacctttcag tgagctgtgc tgctgcttct gtggccagtt
    11161 cacgctcttc tgtattgaaa gactcggaat atggttgttt gaagattcca ccaagatgta
    11221 tgtttgatca tccagatgca gataaaacac tgaatcacct tatatcgggg tttgaaaatt
    11281 ttgaaaagaa aatcaactac agattcaaga ataaggctta ccttctccag gcttttacac
    11341 atgcctccta ccactacaat actatcactg attgttacca gcgcttagaa ttcctgggag
    11401 atgcgatttt ggactacctc ataaccaagc acctttatga agacccgcgg cagcactccc
    11461 cgggggtcct gacagacctg cggtctgccc tggtcaacaa caccatcttt gcatcgctgg
    11521 ctgtaaagta cgactaccac aagtacttca aagctgtctc tcctgagctc ttccatgtca
    11581 ttgatgactt tgtgcagttt cagcttgaga agaatgaaat gcaaggaatg gattctgagc
    11641 ttaggagatc tgaggaggat gaagagaaag aagaggatat tgaagttcca aaggccatgg
    11701 gggatatttt tgagtcgctt gctggtgcca tttacatgga tagtgggatg tcactggaga
    11761 cagtctggca ggtgtactat cccatgatgc ggccactaat agaaaagttt tctgcaaatg
    11821 taccccgttc ccctgtgcga gaattgcttg aaatggaacc agaaactgcc aaatttagcc
    11881 cggctgagag aacttacgac gggaaggtca gagtcactgt ggaagtagta ggaaagggga
    11941 aatttaaagg tgttggtcga agttacagga ttgccaaatc tgcagcagca agaagagccc
    12001 tccgaagcct caaagctaat caacctcagg ttcccaatag ctgactcgag gttaacgaat
    12061 tctacc
//
