LOCUS       sequence-217610-        9871 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpression of the AtCAS9 in plant under 2x35S promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9871)
  TITLE     Chevalier Lab plasmids
REFERENCE   2  (bases 1 to 9871)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pORFE1001
FEATURES             Location/Qualifiers
     source          1..9871
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..346
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     289..309
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             437..4540
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             437..4540
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             4553..4573
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      4589..4841
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    5137..5161
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             complement(5317..6465)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     rep_origin      complement(6801..7389)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6881..6900)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7560..8351,8352..8420))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8183..8202
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8421..8525)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      8612..9243
                     /label=oriV
                     /note="incP origin of replication"
     misc_feature    9335..9359
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 tgagactttt caacaaagga taatttcggg aaacctcctc ggattccatt gcccagctat
       61 ctgtcacttc atcgaaagga cagtagaaaa ggaaggtggc tcctacaaat gccatcattg
      121 cgataaagga aaggctatca ttcaagatct ctctgccgac agtggtccca aagatggacc
      181 cccacccacg aggagcatcg tggaaaaaga agaggttcca accacgtcta caaagcaagt
      241 ggattgatgt gacatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca
      301 agacccttcc tctatataag gaagttcatt tcatttggag aggacacgct cgagtataag
      361 agctcatttt tacaacaatt accaacaaca acaaacaaca aacaacatta caattacatt
      421 tacaattatc gatacaatgg ataagaagta ctctatcgga ctcgatatcg gaactaactc
      481 tgtgggatgg gctgtgatca ccgatgagta caaggtgcca tctaagaagt tcaaggttct
      541 cggaaacacc gataggcact ctatcaagaa aaaccttatc ggtgctctcc tcttcgattc
      601 tggtgaaact gctgaggcta ccagactcaa gagaaccgct agaagaaggt acaccagaag
      661 aaagaacagg atctgctacc tccaagagat cttctctaac gagatggcta aagtggatga
      721 ttcattcttc cacaggctcg aagagtcatt cctcgtggaa gaagataaga agcacgagag
      781 gcaccctatc ttcggaaaca tcgttgatga ggtggcatac cacgagaagt accctactat
      841 ctaccacctc agaaagaagc tcgttgattc tactgataag gctgatctca ggctcatcta
      901 cctcgctctc gctcacatga tcaagttcag aggacacttc ctcatcgagg gtgatctcaa
      961 ccctgataac tctgatgtgg ataagttgtt catccagctc gtgcagacct acaaccagct
     1021 tttcgaagag aaccctatca acgcttcagg tgtggatgct aaggctatcc tctctgctag
     1081 gctctctaag tcaagaaggc ttgagaacct cattgctcag ctccctggtg agaagaagaa
     1141 cggacttttc ggaaacttga tcgctctctc tctcggactc acccctaact tcaagtctaa
     1201 cttcgatctc gctgaggatg caaagctcca gctctcaaag gatacctacg atgatgatct
     1261 cgataacctc ctcgctcaga tcggagatca gtacgctgat ttgttcctcg ctgctaagaa
     1321 cctctctgat gctatcctcc tcagtgatat cctcagagtg aacaccgaga tcaccaaggc
     1381 tccactctca gcttctatga tcaagagata cgatgagcac caccaggatc tcacacttct
     1441 caaggctctt gttagacagc agctcccaga gaagtacaaa gagattttct tcgatcagtc
     1501 taagaacgga tacgctggtt acatcgatgg tggtgcatct caagaagagt tctacaagtt
     1561 catcaagcct atcctcgaga agatggatgg aaccgaggaa ctcctcgtga agctcaatag
     1621 agaggatctt ctcagaaagc agaggacctt cgataacgga tctatccctc atcagatcca
     1681 cctcggagag ttgcacgcta tccttagaag gcaagaggat ttctacccat tcctcaagga
     1741 taacagggaa aagattgaga agattctcac cttcagaatc ccttactacg tgggacctct
     1801 cgctagagga aactcaagat tcgcttggat gaccagaaag tctgaggaaa ccatcacccc
     1861 ttggaacttc gaagaggtgg tggataaggg tgctagtgct cagtctttca tcgagaggat
     1921 gaccaacttc gataagaacc ttccaaacga gaaggtgctc cctaagcact ctttgctcta
     1981 cgagtacttc accgtgtaca acgagttgac caaggttaag tacgtgaccg agggaatgag
     2041 gaagcctgct tttttgtcag gtgagcaaaa gaaggctatc gttgatctct tgttcaagac
     2101 caacagaaag gtgaccgtga agcagctcaa agaggattac ttcaagaaaa tcgagtgctt
     2161 cgattcagtt gagatttctg gtgttgagga taggttcaac gcatctctcg gaacctacca
     2221 cgatctcctc aagatcatta aggataagga tttcttggat aacgaggaaa acgaggatat
     2281 cttggaggat atcgttctta ccctcaccct ctttgaagat agagagatga ttgaagaaag
     2341 gctcaagacc tacgctcatc tcttcgatga taaggtgatg aagcagttga agagaagaag
     2401 atacactggt tggggaaggc tctcaagaaa gctcattaac ggaatcaggg ataagcagtc
     2461 tggaaagaca atccttgatt tcctcaagtc tgatggattc gctaacagaa acttcatgca
     2521 gctcatccac gatgattctc tcacctttaa agaggatatc cagaaggctc aggtttcagg
     2581 acagggtgat agtctccatg agcatatcgc taacctcgct ggatctcctg caatcaagaa
     2641 gggaatcctc cagactgtga aggttgtgga tgagttggtg aaggtgatgg gaaggcataa
     2701 gcctgagaac atcgtgatcg aaatggctag agagaaccag accactcaga agggacagaa
     2761 gaactctagg gaaaggatga agaggatcga ggaaggtatc aaagagcttg gatctcagat
     2821 cctcaaagag caccctgttg agaacactca gctccagaat gagaagctct acctctacta
     2881 cctccagaac ggaagggata tgtatgtgga tcaagagttg gatatcaaca ggctctctga
     2941 ttacgatgtt gatcatatcg tgccacagtc attcttgaag gatgattcta tcgataacaa
     3001 ggtgctcacc aggtctgata agaacagggg taagagtgat aacgtgccaa gtgaagaggt
     3061 tgtgaagaaa atgaagaact attggaggca gctcctcaac gctaagctca tcactcagag
     3121 aaagttcgat aacttgacta aggctgagag gggaggactc tctgaattgg ataaggcagg
     3181 attcatcaag aggcagcttg tggaaaccag gcagatcact aagcacgttg cacagatcct
     3241 cgattctagg atgaacacca agtacgatga gaacgataag ttgatcaggg aagtgaaggt
     3301 tatcaccctc aagtcaaagc tcgtgtctga tttcagaaag gatttccaat tctacaaggt
     3361 gagggaaatc aacaactacc accacgctca cgatgcttac cttaacgctg ttgttggaac
     3421 cgctctcatc aagaagtatc ctaagctcga gtcagagttc gtgtacggtg attacaaggt
     3481 gtacgatgtg aggaagatga tcgctaagtc tgagcaagag atcggaaagg ctaccgctaa
     3541 gtatttcttc tactctaaca tcatgaattt cttcaagacc gagattaccc tcgctaacgg
     3601 tgagatcaga aagaggccac tcatcgagac aaacggtgaa acaggtgaga tcgtgtggga
     3661 taagggaagg gatttcgcta ccgttagaaa ggtgctctct atgccacagg tgaacatcgt
     3721 taagaaaacc gaggtgcaga ccggtggatt ctctaaagag tctatcctcc ctaagaggaa
     3781 ctctgataag ctcattgcta ggaagaagga ttgggaccct aagaaatacg gtggtttcga
     3841 ttctcctacc gtggcttact ctgttctcgt tgtggctaag gttgagaagg gaaagagtaa
     3901 gaagctcaag tctgttaagg aacttctcgg aatcactatc atggaaaggt catctttcga
     3961 gaagaaccca atcgatttcc tcgaggctaa gggatacaaa gaggttaaga aggatctcat
     4021 catcaagctc ccaaagtact cactcttcga actcgagaac ggtagaaaga ggatgctcgc
     4081 ttctgctggt gagcttcaaa agggaaacga gcttgctctc ccatctaagt acgttaactt
     4141 tctttacctc gcttctcact acgagaagtt gaagggatct ccagaagata acgagcagaa
     4201 gcaacttttc gttgagcagc acaagcacta cttggatgag atcatcgagc agatctctga
     4261 gttctctaaa agggtgatcc tcgctgatgc aaacctcgat aaggtgttgt ctgcttacaa
     4321 caagcacaga gataagccta tcagggaaca ggcagagaac atcatccatc tcttcaccct
     4381 taccaacctc ggtgctcctg ctgctttcaa gtacttcgat acaaccatcg ataggaagag
     4441 atacacctct accaaagaag tgctcgatgc taccctcatc catcagtcta tcactggact
     4501 ctacgagact aggatcgatc tctcacagct cggtggtgat tcaagggctg atcctaagaa
     4561 gaagaggaag gtttgagctt gtcaagcaga tcgttcaaac atttggcaat aaagtttctt
     4621 aagattgaat cctgttgccg gtcttgcgat gattatcata taatttctgt tgaattacgt
     4681 taagcatgta ataattaaca tgtaatgcat gacgttattt atgagatggg tttttatgat
     4741 tagagtcccg caattataca tttaatacgc gatagaaaac aaaatatagc gcgcaaacta
     4801 ggataaatta tcgcgcgcgg tgtcatctat gttactagat cgacgcacat ttggcaataa
     4861 agtttcttaa gattgaatcc tgttgccggt cttgcgatga ttatcatata atttctgttg
     4921 aattacgtta agcatgtaat aattaacatg taatgcatga cgttatttat gagatgggtt
     4981 tttatgatta gagtcccgca attatacatt taatacgcga tagaaaacaa aatatagcgc
     5041 gcaaactagg ataaattatc gcgcgcggtg tcatctatgt tactagatcg acgctactat
     5101 tgtcttctgc acgaagtggt ttaaactatc agtgtttgac aggatatatt ggcgggtaaa
     5161 cctaagagaa aagagcgttt attagaataa tcggatattt aaaagggcgt gaaaaggttt
     5221 atccgttcgt ccatttgtat gtgcatgcca accacagggt tccccagatc aggcgctggc
     5281 tgctgaaccc ccagccggaa ctgaccccac aaggccctag cgtttgcaat gcaccaggtc
     5341 atcattgacc caggcgtgtt ccaccaggcc gctgcctcgc aactcttcgc aggcttcgcc
     5401 gacctgctcg cgccacttct tcacgcgggt ggaatccgat ccgcacatga ggcggaaggt
     5461 ttccagcttg agcgggtacg gctcccggtg cgagctgaaa tagtcgaaca tccgtcgggc
     5521 cgtcggcgac agcttgcggt acttctccca tatgaatttc gtgtagtggt cgccagcaaa
     5581 cagcacgacg atttcctcgt cgatcaggac ctggcaacgg gacgttttct tgccacggtc
     5641 caggacgcgg aagcggtgca gcagcgacac cgattccagg tgcccaacgc ggtcggacgt
     5701 gaagcccatc gccgtcgcct gtaggcgcga caggcattcc tcggccttcg tgtaataccg
     5761 gccattgatc gaccagccca ggtcctggca aagctcgtag aacgtgaagg tgatcggctc
     5821 gccgataggg gtgcgcttcg cgtactccaa cacctgctgc cacaccagtt cgtcatcgtc
     5881 ggcccgcagc tcgacgccgg tgtaggtgat cttcacgtcc ttgttgacgt ggaaaatgac
     5941 cttgttttgc agcgcctcgc gcgggatttt cttgttgcgc gtggtgaaca gggcagagcg
     6001 ggccgtgtcg tttggcatcg ctcgcatcgt gtccggccac ggcgcaatat cgaacaagga
     6061 aagctgcatt tccttgatct gctgcttcgt gtgtttcagc aacgcggcct gcttggcctc
     6121 gctgacctgt tttgccaggt cctcgccggc ggtttttcgc ttcttggtcg tcatagttcc
     6181 tcgcgtgtcg atggtcatcg acttcgccaa acctgccgcc tcctgttcaa gacgacgcga
     6241 acgctccacg gcggccgatg gcgcgggcag ggcaggggga gccagttgca cgctgtcgcg
     6301 ctcgatcttg gccgtagctt gctggaccat cgagccgacg gactggaagg tttcgcgggg
     6361 cgcacgcatg acggtgcggc ttgcgatggt ttcggcatcc tcggcggaaa accccgcgtc
     6421 gatcagttct tgcctgtatg ccttccggtc aaacgtccga ttcattcacc ctccttgcgg
     6481 gattgccccg actcacgccg gggcaatgtg cccttattcc tgatttgacc cgcctggtgc
     6541 cttggtgtcc agataatcca ccttatcggc aatgaagtcg gtcccgtaga ccgtctggcc
     6601 gtccttctcg tacttggtat tccgaatctt gccctgcacg aataccagcg accccttgcc
     6661 caaatacttg ccgtgggcct cggcctgaga gccaaaacac ttgatgcgga agaagtcggt
     6721 gcgctcctgc ttgtcgccgg catcgttgcg ccacatctag gatctgccag gaaccgtaaa
     6781 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     6841 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     6901 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     6961 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     7021 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     7081 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     7141 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     7201 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     7261 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     7321 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     7381 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     7441 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     7501 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     7561 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     7621 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     7681 agtgctgcaa tgataccgcg agaaccacgc tcaccggctc cagatttatc agcaataaac
     7741 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     7801 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     7861 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     7921 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     7981 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     8041 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     8101 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     8161 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     8221 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     8281 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     8341 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     8401 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcaaggt
     8461 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     8521 ccgcgcacga attggccagc gctgccattt ttggggtgag gccgttcgcg gccgaggggc
     8581 gcagcccctg gggggatggg aggcccgcgt tagcgggccg ggagggttcg agaagggggg
     8641 gcacccccct tcggcgtgcg cggtcacgcg cacagggcgc agccctggtt aaaaacaagg
     8701 tttataaata ttggtttaaa agcaggttaa aagacaggtt agcggtggcc gaaaaacggg
     8761 cggaaaccct tgcaaatgct ggattttctg cctgtggaca gcccctcaaa tgtcaatagg
     8821 tgcgcccctc atctgtcagc actctgcccc tcaagtgtca aggatcgcgc ccctcatctg
     8881 tcagtagtcg cgcccctcaa gtgtcaatac cgcagggcac ttatccccag gcttgtccac
     8941 atcatctgtg ggaaactcgc gtaaaatcag gcgttttcgc cgatttgcga ggctggccag
     9001 ctccacgtcg ccggccgaaa tcgagcctgc ccctcatctg tcaacgccgc gccgggtgag
     9061 tcggcccctc aagtgtcaac gtccgcccct catctgtcag tgagggccaa gttttccgcg
     9121 aggtatccac aacgccggcg gccgcggtgt ctcgcacacg gcttcgacgg cgtttctggc
     9181 gcgtttgcag ggccatagac ggccgccagc ccagcggcga gggcaaccag cccggtgagc
     9241 gtcgcaaagg agatcctgat ctgactgatg ggctgcctgt atcgagtggt gattttgtgc
     9301 cgagctgccg gtcggggagc tgttggctgg ctggtggcag gatatattgt ggtgtaaaca
     9361 aattgacgct tagacaactt aataacacat tgcggacgtt tttaatgtac tggggtggat
     9421 gcagtgggcc ccactctgtg aagacaagca agaattcaag cttggaggtc aacatggtgg
     9481 agcacgacac tctggtctac tccaaaaatg tcaaagatac agtctcagaa gatcaaaggg
     9541 ctattgagac ttttcaacaa aggataattt cgggaaacct cctcggattc cattgcccag
     9601 ctatctgtca cttcatcgaa aggacagtag aaaaggaagg tggctcctac aaatgccatc
     9661 attgcgataa aggaaaggct atcattcaag atctctctgc cgacagtggt cccaaagatg
     9721 gacccccacc cacgaggagc atcgtggaaa aagaagaggt tccaaccacg tctacaaagc
     9781 aagtggattg atgtgataac atggtggagc acgacactct ggtctactcc aaaaatgtca
     9841 aagatacagt ctcagaagat caaagggcta t
//
