LOCUS       sequence-217613-        9872 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpression of the AtCAS9 nickase in plant under 2x35S promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9872)
  TITLE     Chevalier Lab plasmids
REFERENCE   2  (bases 1 to 9872)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pORFE1052
FEATURES             Location/Qualifiers
     source          1..9872
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..346
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     289..309
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             join(437..463,464..466,467..4540)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             4553..4573
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      4589..4841
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      4838..5090
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    5138..5162
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             complement(5318..6466)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     rep_origin      complement(6802..7390)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6882..6901)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7561..8352,8353..8421))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8184..8203
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8422..8526)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      8613..9244
                     /label=oriV
                     /note="incP origin of replication"
     misc_feature    9336..9360
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 tgagactttt caacaaagga taatttcggg aaacctcctc ggattccatt gcccagctat
       61 ctgtcacttc atcgaaagga cagtagaaaa ggaaggtggc tcctacaaat gccatcattg
      121 cgataaagga aaggctatca ttcaagatct ctctgccgac agtggtccca aagatggacc
      181 cccacccacg aggagcatcg tggaaaaaga agaggttcca accacgtcta caaagcaagt
      241 ggattgatgt gacatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca
      301 agacccttcc tctatataag gaagttcatt tcatttggag aggacacgct cgagtataag
      361 agctcatttt tacaacaatt accaacaaca acaaacaaca aacaacatta caattacatt
      421 tacaattatc gatacaatgg ataagaagta ctctatcgga ctcgctatcg gaactaactc
      481 tgtgggatgg gctgtgatca ccgatgagta caaggtgcca tctaagaagt tcaaggttct
      541 cggaaacacc gataggcact ctatcaagaa aaaccttatc ggtgctctcc tcttcgattc
      601 tggtgaaact gctgaggcta ccagactcaa gagaaccgct agaagaaggt acaccagaag
      661 aaagaacagg atctgctacc tccaagagat cttctctaac gagatggcta aagtggatga
      721 ttcattcttc cacaggctcg aagagtcatt cctcgtggaa gaagataaga agcacgagag
      781 gcaccctatc ttcggaaaca tcgttgatga ggtggcatac cacgagaagt accctactat
      841 ctaccacctc agaaagaagc tcgttgattc tactgataag gctgatctca ggctcatcta
      901 cctcgctctc gctcacatga tcaagttcag aggacacttc ctcatcgagg gtgatctcaa
      961 ccctgataac tctgatgtgg ataagttgtt catccagctc gtgcagacct acaaccagct
     1021 tttcgaagag aaccctatca acgcttcagg tgtggatgct aaggctatcc tctctgctag
     1081 gctctctaag tcaagaaggc ttgagaacct cattgctcag ctccctggtg agaagaagaa
     1141 cggacttttc ggaaacttga tcgctctctc tctcggactc acccctaact tcaagtctaa
     1201 cttcgatctc gctgaggatg caaagctcca gctctcaaag gatacctacg atgatgatct
     1261 cgataacctc ctcgctcaga tcggagatca gtacgctgat ttgttcctcg ctgctaagaa
     1321 cctctctgat gctatcctcc tcagtgatat cctcagagtg aacaccgaga tcaccaaggc
     1381 tccactctca gcttctatga tcaagagata cgatgagcac caccaggatc tcacacttct
     1441 caaggctctt gttagacagc agctcccaga gaagtacaaa gagattttct tcgatcagtc
     1501 taagaacgga tacgctggtt acatcgatgg tggtgcatct caagaagagt tctacaagtt
     1561 catcaagcct atcctcgaga agatggatgg aaccgaggaa ctcctcgtga agctcaatag
     1621 agaggatctt ctcagaaagc agaggacctt cgataacgga tctatccctc atcagatcca
     1681 cctcggagag ttgcacgcta tccttagaag gcaagaggat ttctacccat tcctcaagga
     1741 taacagggaa aagattgaga agattctcac cttcagaatc ccttactacg tgggacctct
     1801 cgctagagga aactcaagat tcgcttggat gaccagaaag tctgaggaaa ccatcacccc
     1861 ttggaacttc gaagaggtgg tggataaggg tgctagtgct cagtctttca tcgagaggat
     1921 gaccaacttc gataagaacc ttccaaacga gaaggtgctc cctaagcact ctttgctcta
     1981 cgagtacttc accgtgtaca acgagttgac caaggttaag tacgtgaccg agggaatgag
     2041 gaagcctgct tttttgtcag gtgagcaaaa gaaggctatc gttgatctct tgttcaagac
     2101 caacagaaag gtgaccgtga agcagctcaa agaggattac ttcaagaaaa tcgagtgctt
     2161 cgattcagtt gagatttctg gtgttgagga taggttcaac gcatctctcg gaacctacca
     2221 cgatctcctc aagatcatta aggataagga tttcttggat aacgaggaaa acgaggatat
     2281 cttggaggat atcgttctta ccctcaccct ctttgaagat agagagatga ttgaagaaag
     2341 gctcaagacc tacgctcatc tcttcgatga taaggtgatg aagcagttga agagaagaag
     2401 atacactggt tggggaaggc tctcaagaaa gctcattaac ggaatcaggg ataagcagtc
     2461 tggaaagaca atccttgatt tcctcaagtc tgatggattc gctaacagaa acttcatgca
     2521 gctcatccac gatgattctc tcacctttaa agaggatatc cagaaggctc aggtttcagg
     2581 acagggtgat agtctccatg agcatatcgc taacctcgct ggatctcctg caatcaagaa
     2641 gggaatcctc cagactgtga aggttgtgga tgagttggtg aaggtgatgg gaaggcataa
     2701 gcctgagaac atcgtgatcg aaatggctag agagaaccag accactcaga agggacagaa
     2761 gaactctagg gaaaggatga agaggatcga ggaaggtatc aaagagcttg gatctcagat
     2821 cctcaaagag caccctgttg agaacactca gctccagaat gagaagctct acctctacta
     2881 cctccagaac ggaagggata tgtatgtgga tcaagagttg gatatcaaca ggctctctga
     2941 ttacgatgtt gatcatatcg tgccacagtc attcttgaag gatgattcta tcgataacaa
     3001 ggtgctcacc aggtctgata agaacagggg taagagtgat aacgtgccaa gtgaagaggt
     3061 tgtgaagaaa atgaagaact attggaggca gctcctcaac gctaagctca tcactcagag
     3121 aaagttcgat aacttgacta aggctgagag gggaggactc tctgaattgg ataaggcagg
     3181 attcatcaag aggcagcttg tggaaaccag gcagatcact aagcacgttg cacagatcct
     3241 cgattctagg atgaacacca agtacgatga gaacgataag ttgatcaggg aagtgaaggt
     3301 tatcaccctc aagtcaaagc tcgtgtctga tttcagaaag gatttccaat tctacaaggt
     3361 gagggaaatc aacaactacc accacgctca cgatgcttac cttaacgctg ttgttggaac
     3421 cgctctcatc aagaagtatc ctaagctcga gtcagagttc gtgtacggtg attacaaggt
     3481 gtacgatgtg aggaagatga tcgctaagtc tgagcaagag atcggaaagg ctaccgctaa
     3541 gtatttcttc tactctaaca tcatgaattt cttcaagacc gagattaccc tcgctaacgg
     3601 tgagatcaga aagaggccac tcatcgagac aaacggtgaa acaggtgaga tcgtgtggga
     3661 taagggaagg gatttcgcta ccgttagaaa ggtgctctct atgccacagg tgaacatcgt
     3721 taagaaaacc gaggtgcaga ccggtggatt ctctaaagag tctatcctcc ctaagaggaa
     3781 ctctgataag ctcattgcta ggaagaagga ttgggaccct aagaaatacg gtggtttcga
     3841 ttctcctacc gtggcttact ctgttctcgt tgtggctaag gttgagaagg gaaagagtaa
     3901 gaagctcaag tctgttaagg aacttctcgg aatcactatc atggaaaggt catctttcga
     3961 gaagaaccca atcgatttcc tcgaggctaa gggatacaaa gaggttaaga aggatctcat
     4021 catcaagctc ccaaagtact cactcttcga actcgagaac ggtagaaaga ggatgctcgc
     4081 ttctgctggt gagcttcaaa agggaaacga gcttgctctc ccatctaagt acgttaactt
     4141 tctttacctc gcttctcact acgagaagtt gaagggatct ccagaagata acgagcagaa
     4201 gcaacttttc gttgagcagc acaagcacta cttggatgag atcatcgagc agatctctga
     4261 gttctctaaa agggtgatcc tcgctgatgc aaacctcgat aaggtgttgt ctgcttacaa
     4321 caagcacaga gataagccta tcagggaaca ggcagagaac atcatccatc tcttcaccct
     4381 taccaacctc ggtgctcctg ctgctttcaa gtacttcgat acaaccatcg ataggaagag
     4441 atacacctct accaaagaag tgctcgatgc taccctcatc catcagtcta tcactggact
     4501 ctacgagact aggatcgatc tctcacagct cggtggtgat tcaagggctg atcctaagaa
     4561 gaagaggaag gtttgagctt gtcaagcaga tcgttcaaac atttggcaat aaagtttctt
     4621 aagattgaat cctgttgccg gtcttgcgat gattatcata taatttctgt tgaattacgt
     4681 taagcatgta ataattaaca tgtaatgcat gacgttattt atgagatggg tttttatgat
     4741 tagagtcccg caattataca tttaatacgc gatagaaaac aaaatatagc gcgcaaacta
     4801 ggataaatta tcgcgcgcgg tgtcatctat gttactagat cgttcaaaca tttggcaata
     4861 aagtttctta agattgaatc ctgttgccgg tcttgcgatg attatcatat aatttctgtt
     4921 gaattacgtt aagcatgtaa taattaacat gtaatgcatg acgttattta tgagatgggt
     4981 ttttatgatt agagtcccgc aattatacat ttaatacgcg atagaaaaca aaatatagcg
     5041 cgcaaactag gataaattat cgcgcgcggt gtcatctatg ttactagatc gacgctacta
     5101 ttgtcttctg cacgaagtgg tttaaactat cagtgtttga caggatatat tggcgggtaa
     5161 acctaagaga aaagagcgtt tattagaata atcggatatt taaaagggcg tgaaaaggtt
     5221 tatccgttcg tccatttgta tgtgcatgcc aaccacaggg ttccccagat caggcgctgg
     5281 ctgctgaacc cccagccgga actgacccca caaggcccta gcgtttgcaa tgcaccaggt
     5341 catcattgac ccaggcgtgt tccaccaggc cgctgcctcg caactcttcg caggcttcgc
     5401 cgacctgctc gcgccacttc ttcacgcggg tggaatccga tccgcacatg aggcggaagg
     5461 tttccagctt gagcgggtac ggctcccggt gcgagctgaa atagtcgaac atccgtcggg
     5521 ccgtcggcga cagcttgcgg tacttctccc atatgaattt cgtgtagtgg tcgccagcaa
     5581 acagcacgac gatttcctcg tcgatcagga cctggcaacg ggacgttttc ttgccacggt
     5641 ccaggacgcg gaagcggtgc agcagcgaca ccgattccag gtgcccaacg cggtcggacg
     5701 tgaagcccat cgccgtcgcc tgtaggcgcg acaggcattc ctcggccttc gtgtaatacc
     5761 ggccattgat cgaccagccc aggtcctggc aaagctcgta gaacgtgaag gtgatcggct
     5821 cgccgatagg ggtgcgcttc gcgtactcca acacctgctg ccacaccagt tcgtcatcgt
     5881 cggcccgcag ctcgacgccg gtgtaggtga tcttcacgtc cttgttgacg tggaaaatga
     5941 ccttgttttg cagcgcctcg cgcgggattt tcttgttgcg cgtggtgaac agggcagagc
     6001 gggccgtgtc gtttggcatc gctcgcatcg tgtccggcca cggcgcaata tcgaacaagg
     6061 aaagctgcat ttccttgatc tgctgcttcg tgtgtttcag caacgcggcc tgcttggcct
     6121 cgctgacctg ttttgccagg tcctcgccgg cggtttttcg cttcttggtc gtcatagttc
     6181 ctcgcgtgtc gatggtcatc gacttcgcca aacctgccgc ctcctgttca agacgacgcg
     6241 aacgctccac ggcggccgat ggcgcgggca gggcaggggg agccagttgc acgctgtcgc
     6301 gctcgatctt ggccgtagct tgctggacca tcgagccgac ggactggaag gtttcgcggg
     6361 gcgcacgcat gacggtgcgg cttgcgatgg tttcggcatc ctcggcggaa aaccccgcgt
     6421 cgatcagttc ttgcctgtat gccttccggt caaacgtccg attcattcac cctccttgcg
     6481 ggattgcccc gactcacgcc ggggcaatgt gcccttattc ctgatttgac ccgcctggtg
     6541 ccttggtgtc cagataatcc accttatcgg caatgaagtc ggtcccgtag accgtctggc
     6601 cgtccttctc gtacttggta ttccgaatct tgccctgcac gaataccagc gaccccttgc
     6661 ccaaatactt gccgtgggcc tcggcctgag agccaaaaca cttgatgcgg aagaagtcgg
     6721 tgcgctcctg cttgtcgccg gcatcgttgc gccacatcta ggatctgcca ggaaccgtaa
     6781 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     6841 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     6901 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     6961 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     7021 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     7081 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     7141 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     7201 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     7261 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     7321 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     7381 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     7441 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     7501 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     7561 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     7621 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     7681 cagtgctgca atgataccgc gagaaccacg ctcaccggct ccagatttat cagcaataaa
     7741 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     7801 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     7861 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     7921 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     7981 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     8041 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     8101 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     8161 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     8221 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     8281 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     8341 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     8401 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaagg
     8461 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     8521 tccgcgcacg aattggccag cgctgccatt tttggggtga ggccgttcgc ggccgagggg
     8581 cgcagcccct ggggggatgg gaggcccgcg ttagcgggcc gggagggttc gagaaggggg
     8641 ggcacccccc ttcggcgtgc gcggtcacgc gcacagggcg cagccctggt taaaaacaag
     8701 gtttataaat attggtttaa aagcaggtta aaagacaggt tagcggtggc cgaaaaacgg
     8761 gcggaaaccc ttgcaaatgc tggattttct gcctgtggac agcccctcaa atgtcaatag
     8821 gtgcgcccct catctgtcag cactctgccc ctcaagtgtc aaggatcgcg cccctcatct
     8881 gtcagtagtc gcgcccctca agtgtcaata ccgcagggca cttatcccca ggcttgtcca
     8941 catcatctgt gggaaactcg cgtaaaatca ggcgttttcg ccgatttgcg aggctggcca
     9001 gctccacgtc gccggccgaa atcgagcctg cccctcatct gtcaacgccg cgccgggtga
     9061 gtcggcccct caagtgtcaa cgtccgcccc tcatctgtca gtgagggcca agttttccgc
     9121 gaggtatcca caacgccggc ggccgcggtg tctcgcacac ggcttcgacg gcgtttctgg
     9181 cgcgtttgca gggccataga cggccgccag cccagcggcg agggcaacca gcccggtgag
     9241 cgtcgcaaag gagatcctga tctgactgat gggctgcctg tatcgagtgg tgattttgtg
     9301 ccgagctgcc ggtcggggag ctgttggctg gctggtggca ggatatattg tggtgtaaac
     9361 aaattgacgc ttagacaact taataacaca ttgcggacgt ttttaatgta ctggggtgga
     9421 tgcagtgggc cccactctgt gaagacaagc aagaattcaa gcttggaggt caacatggtg
     9481 gagcacgaca ctctggtcta ctccaaaaat gtcaaagata cagtctcaga agatcaaagg
     9541 gctattgaga cttttcaaca aaggataatt tcgggaaacc tcctcggatt ccattgccca
     9601 gctatctgtc acttcatcga aaggacagta gaaaaggaag gtggctccta caaatgccat
     9661 cattgcgata aaggaaaggc tatcattcaa gatctctctg ccgacagtgg tcccaaagat
     9721 ggacccccac ccacgaggag catcgtggaa aaagaagagg ttccaaccac gtctacaaag
     9781 caagtggatt gatgtgataa catggtggag cacgacactc tggtctactc caaaaatgtc
     9841 aaagatacag tctcagaaga tcaaagggct at
//
