LOCUS       sequence-217614-       10419 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A cytidine deaminase-mediated base-editing plasmid in Pseudomonas 
            species.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10419)
  AUTHORS   Chen W, Zhang Y, Zhang Y, Pi Y, Gu T, Song L, Wang Y, Ji Q
  TITLE     CRISPR/Cas9-based Genome Editing in Pseudomonas aeruginosa and 
            Cytidine Deaminase-Mediated Base Editing in Pseudomonas Species.
  JOURNAL   iScience. 2018 Aug 31;6:222-231. doi: 10.1016/j.isci.2018.07.024. 
            Epub 2018 Aug 1.
  PUBMED    30240613
REFERENCE   2  (bases 1 to 10419)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "iScience.";
            date: "2018-08-31"; volume: "6:222-231. doi"; pages: " 
            10.1016/j.isci.2018.07.024. Epub 2018 Aug 1"
COMMENT     Sequence Label: pnCasPA-BEC
FEATURES             Location/Qualifiers
     source          1..10419
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(1..6,7..23,24..30)
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     misc_RNA        53..128
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             323..1009
                     /codon_start=1
                     /product="cytidine deaminase (C to U editing enzyme) from 
                     rat"
                     /label=APOBEC-1
                     /note="can use ssDNA as a substrate (Komor et al., 2016)"
                     /translation="MSSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWG
                     GRHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFL
                     SRYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSN
                     EAHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHI
                     LWATGLK"
     CDS             join(1058..1081,1082..1084,1085..5161)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     promoter        join(5283..5288,5289..5305,5306..5311)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             5500..6033
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     rep_origin      6331..6682
                     /label=pRO1600 oriV
                     /note="broad-host-range origin of replication from 
                     Pseudomonas aeruginosa plasmid pRO1600; requires the 
                     pRO1600 Rep protein for replication (West et al., 1994)"
     terminator      7700..7794
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      7882..8470
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8371..8390
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8561..8580
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     CDS             complement(join(8619..9953,9954..10040))
                     /codon_start=1
                     /gene="Bacillus subtilis sacB"
                     /product="secreted levansucrase that renders bacterial 
                     growth sensitive to sucrose"
                     /label=SacB
                     /note="negative selection marker"
                     /translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISH
                     ITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYH
                     IVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEW
                     SGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDG
                     KTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKA
                     YYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDE
                     IERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLK
                     MDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSV
                     VKDSILEQGQLTVNK"
ORIGIN
        1 ttgacaatta atcatccggc tcgtataatg tgtggcgaga ccattggtct cagttttaga
       61 gctagaaata gcaagttaaa ataaggctag tccgttatca acttgaaaaa gtggcaccga
      121 gtcggtgctt tttttgagat ctgtccatac ccatggtcta gaatgctcga ggcatcaacg
      181 acaagccccg gctatcccga tagccggggc tttgtcttga cggtgtacaa gaacctcttt
      241 actattttgt accccgaaaa ttggcagggc gcacgctctg tcatttcgtt tgtgtgtcgt
      301 aagacaatca gtggagctat agatgagtag cgaaaccggt ccggttgcag tggatccgac
      361 cctgcgccgt cgcattgaac cgcacgagtt tgaagtgttc tttgatccgc gcgagctgcg
      421 caaagaaact tgcctgctgt acgagattaa ctggggtggc cgccatagca tctggcgcca
      481 taccagccag aacaccaata agcacgtgga agtgaatttt attgaaaaat ttaccaccga
      541 gcgctacttc tgccctaata cccgctgcag catcacctgg tttctgagct ggagcccgtg
      601 cggcgaatgt agtcgcgcca ttaccgagtt cctgagccgc tatccgcatg tgaccctgtt
      661 catctacatc gcccgtctgt accatcacgc cgatccgcgc aatcgccaag gtctgcgtga
      721 tctgattagc agcggtgtga ccatccagat catgaccgaa caagagagcg gctattgctg
      781 gcgcaacttc gtgaactatt ctccgagcaa cgaagcccac tggccgcgtt atccgcatct
      841 gtgggtgcgc ctgtatgtgc tggagctgta ctgcatcatc ctgggcctgc cgccttgcct
      901 gaatattctg cgccgtaaac agccgcaact gacattcttc accatcgcac tgcagagctg
      961 ccattatcag cgcctgccgc cgcacatttt atgggccacc ggtctgaaaa gcggtagtga
     1021 aactccgggc acaagcgaaa gcgcaacccc ggaaagtgat aagaaatact caataggctt
     1081 agctatcggc acaaatagcg tcggatgggc ggtgatcact gatgaatata aggttccgtc
     1141 taaaaagttc aaggttctgg gaaatacaga ccgccacagt atcaaaaaaa atcttatagg
     1201 ggctctttta tttgacagtg gagagacagc ggaagcgact cgtctcaaac ggacagctcg
     1261 tagaaggtat acacgtcgga agaatcgtat ttgttatcta caggagattt tttcaaatga
     1321 gatggcgaaa gtagatgata gtttctttca tcgacttgaa gagtcttttt tggtggaaga
     1381 agacaagaag catgaacgtc atcctatttt tggaaatata gtagatgaag ttgcttatca
     1441 tgagaaatat ccaactatct atcatctgcg aaaaaaattg gtagattcta ctgataaagc
     1501 ggatttgcgc ttaatctatt tggccttagc gcatatgatt aagtttcgtg gtcatttttt
     1561 gattgaggga gatttaaatc ctgataatag tgatgtggac aaactattta tccagttggt
     1621 acaaacctac aatcaattat ttgaagaaaa ccctattaac gcaagtggag tagatgctaa
     1681 agcgattctt tctgcacgat tgagtaaatc aagacgatta gaaaatctca ttgctcagct
     1741 ccccggtgag aagaaaaatg gcttatttgg gaatctcatt gctttgtcat tgggtttgac
     1801 ccctaatttt aaatcaaatt ttgatttggc agaagatgct aaattacagc tttcaaaaga
     1861 tacttacgat gatgatttag ataatttatt ggcgcaaatt ggagatcaat atgctgattt
     1921 gtttttggca gctaagaatt tatcagatgc tattttactt tcagatatcc taagagtaaa
     1981 tactgaaata actaaggctc ccctatcagc ttcaatgatt aaacgctacg atgaacatca
     2041 tcaagacttg actcttttaa aagctttagt tcgacaacaa cttccagaaa agtataaaga
     2101 aatctttttt gatcaatcaa aaaacggata tgcaggttat attgatgggg gagctagcca
     2161 agaagaattt tataaattta tcaaaccaat tttagaaaaa atggatggta ctgaggaatt
     2221 attggtgaaa ctaaatcgtg aagatttgct gcgcaagcaa cggacctttg acaacggctc
     2281 tattccccat caaattcact tgggtgagct gcatgctatt ttgagaagac aagaagactt
     2341 ttatccattt ttaaaagaca atcgtgagaa gattgaaaaa atcttgactt ttcgaattcc
     2401 ttattatgtt ggtccattgg cgcgtggcaa tagtcgtttt gcatggatga ctcggaagtc
     2461 tgaagaaaca attaccccat ggaattttga agaagttgtc gataaaggtg cttcagctca
     2521 atcatttatt gaacgcatga caaactttga taaaaatctt ccaaatgaaa aagtactacc
     2581 aaaacatagt ttgctttatg agtattttac ggtttataac gaattgacaa aggtcaaata
     2641 tgttactgaa ggaatgcgaa aaccagcatt tctttcaggt gaacagaaga aagccattgt
     2701 tgatttactc ttcaaaacaa atcgaaaagt aaccgttaag caattaaaag aagattattt
     2761 caaaaaaata gaatgttttg atagtgttga aatttcagga gttgaagata gatttaatgc
     2821 ttcattaggt acctaccatg atttgctaaa aattattaaa gataaagatt ttttggataa
     2881 tgaagaaaat gaagatatct tagaggatat tgttttaaca ttgaccttat ttgaagatag
     2941 ggagatgatt gaggaaagac ttaaaacata tgctcacctc tttgatgata aggtgatgaa
     3001 acagcttaaa cgtcgccgtt atactggttg gggacgtttg tctcgaaaat tgattaatgg
     3061 tattagggat aagcaatctg gcaaaacaat attagatttt ttgaaatcag atggttttgc
     3121 caatcgcaat tttatgcagc tgatccatga tgatagtttg acatttaaag aagacattca
     3181 aaaagcacaa gtgtctggac aaggcgatag tttacatgaa catattgcaa atttagctgg
     3241 tagccctgct attaaaaaag gtattttaca gactgtaaaa gttgttgatg aattggtcaa
     3301 agtaatgggg cggcataagc cagaaaatat cgttattgaa atggcacgtg aaaatcagac
     3361 aactcaaaag ggccagaaaa attcgcgaga gcgtatgaaa cgaatcgaag aaggtatcaa
     3421 agaattagga agtcagattc ttaaagagca tcctgttgaa aatactcaat tgcaaaatga
     3481 aaagctctat ctctattatc tccaaaatgg aagagacatg tatgtggacc aagaattaga
     3541 tattaatcgt ttaagtgatt atgatgtcga tcacattgtt ccacaaagtt tccttaaaga
     3601 cgattcaata gacaataagg tcttaacgcg ttctgataaa aatcgtggta aatcggataa
     3661 cgttccaagt gaagaagtag tcaaaaagat gaaaaactat tggagacaac ttctaaacgc
     3721 caagttaatc actcaacgta agtttgataa tttaacgaaa gctgaacgtg gaggtttgag
     3781 tgaacttgat aaagctggtt ttatcaaacg ccaattggtt gaaactcgcc aaatcactaa
     3841 gcatgtggca caaattttgg atagtcgcat gaatactaaa tacgatgaaa atgataaact
     3901 tattcgagag gttaaagtga ttaccttaaa atctaaatta gtttctgact tccgaaaaga
     3961 tttccaattc tataaagtac gtgagattaa caattaccat catgcccatg atgcgtatct
     4021 aaatgccgtc gttggaactg ctttgattaa gaaatatcca aaacttgaat cggagtttgt
     4081 ctatggtgat tataaagttt atgatgttcg taaaatgatt gctaagtctg agcaagaaat
     4141 aggcaaagca accgcaaaat atttctttta ctctaatatc atgaacttct tcaaaacaga
     4201 aattacactt gcaaatggag agattcgcaa acgccctcta atcgaaacta atggggaaac
     4261 tggagaaatt gtctgggata aagggcgaga ttttgccaca gtgcgcaaag tattgtccat
     4321 gccccaagtc aatattgtca agaaaacaga agtacagaca ggcggattct ccaaggagtc
     4381 aattttacca aaaagaaatt cggacaagct tattgctcgt aaaaaagact gggatccaaa
     4441 aaaatatggt ggttttgata gtccaacggt agcttattca gtcctagtgg ttgctaaggt
     4501 ggaaaaaggg aaatcgaaga agttaaaatc cgttaaagag ttactaggga tcacaattat
     4561 ggaaagaagt tcctttgaaa aaaatccgat tgacttttta gaagctaaag gatataagga
     4621 agttaaaaaa gacttaatca ttaaactacc taaatatagt ctttttgagt tagaaaacgg
     4681 tcgtaaacgg atgctggcta gtgccggaga attacaaaaa ggaaatgagc tggctctgcc
     4741 aagcaaatat gtgaattttt tatatttagc tagtcattat gaaaagttga agggtagtcc
     4801 agaagataac gaacaaaaac aattgtttgt ggagcagcat aagcattatt tagatgagat
     4861 tattgagcaa atcagtgaat tttctaagcg tgttatttta gcagatgcca atttagataa
     4921 agttcttagt gcatataaca aacatagaga caaaccaata cgtgaacaag cagaaaatat
     4981 tattcattta tttacgttga cgaatcttgg agctcccgct gcttttaaat attttgatac
     5041 aacaattgat cgtaaacgat atacgtctac aaaagaagtt ttagatgcca ctcttatcca
     5101 tcaatccatc actggtcttt atgaaacacg cattgatttg agtcagctag gaggtgactg
     5161 atggctggtt ggcgtactgt tgtggtaaat acccatgggt atggacagtt ttcccgaata
     5221 ggaacttcgg aataggaact tcaagatccc ctgattccct ttgtcaacag caatggatcg
     5281 aattgacata agcctgttcg gttcgtaaac tgtaatgcaa gtagcgtatg cgctcacgca
     5341 actggtccag aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg gttttcatgg
     5401 cttgttatga ctgttttttt gtacagtcta tgcctcgggc atccaagcag caagcgcgtt
     5461 acgccgtggg tcgatgtttg atgttatgga gcagcaacga tgttacgcag cagcaacgat
     5521 gttacgcagc agggcagtcg ccctaaaaca aagttaggtg gctcaagtat gggcatcatt
     5581 cgcacatgta ggctcggccc tgaccaagtc aaatccatgc gggctgctct tgatcttttc
     5641 ggtcgtgagt tcggagacgt agccacctac tcccaacatc agccggactc cgattacctc
     5701 gggaacttgc tccgtagtaa gacattcatc gcgcttgctg ccttcgacca agaagcggtt
     5761 gttggcgctc tcgcggctta cgttctgccc aagtttgagc agccgcgtag tgagatctat
     5821 atctatgatc tcgcagtctc cggcgagcac cggaggcagg gcattgccac cgcgctcatc
     5881 aatctcctca agcatgaggc caacgcgctt ggtgcttatg tgatctacgt gcaagcagat
     5941 tacggtgacg atcccgcagt ggctctctat acaaagttgg gcatacggga agaagtgatg
     6001 cactttgata tcgacccaag taccgccacc taacaattcg ttcaagccga gatcagcttc
     6061 ccggccgcgg agttgttcgg taaattgtca caacgccgcg gccaattcga gagttttcgt
     6121 tccactgagc gtcagacccc aattacacgc cactggctgt gcttgctggg gtgacggtgg
     6181 caacggtggc ggccttgctg ggctatcgcg ttggaaagaa acgagggaaa ggggactgat
     6241 aaaccggtct tagcccctcc ccttggtgtc caaccgctct gtaggcctct caggcgccgc
     6301 tggtgccgct ggttggacgc caagggtgaa tccgcctcga taccctgatt actcgcttcc
     6361 tgcgccctct caggcggcga taggggactg gtaaaacggg gattgcccag acgcctcccc
     6421 cgccccttca ggggcacaaa tgcggcccca acggggccac gtagtggtgc gttttttgcg
     6481 tttccaccct tttcttcctt ttccctttta aaccttttag gacgtctaca ggccacgtaa
     6541 tccgtggcct gtagagttta aaaagggacg gatttgttgc cattaaggga cggatttgtt
     6601 gttaagaagg gacggatttg ttgttgtaaa gggacggatt tgttgtattg tgggacgcag
     6661 atacagtgtc cccttataca caaggaatgt cgaacgtggc ctcaccccca atggtttaca
     6721 aaagcaatgc cctggtcgag gccgcgtatc gcctcagtgt tcaggaacag cggatcgttc
     6781 tggcctgtat tagccaggtg aagaggagcg agcctgtcac cgatgaagtg atgtattcag
     6841 tgacggcgga ggacatagcg acgatggcgg gtgtccctat cgaatcttcc tacaaccagc
     6901 tcaaagaagc ggccctgcgc ctgaaacggc gggaagtccg gttaacccaa gagcccaatg
     6961 gcaaggggaa aagaccgagt gtgatgatta ccggctgggt gcaaacaatc atctaccggg
     7021 agggtgaggg ccgtgtagaa ctcaggttca ccaaagacat gctgccgtac ctgacggaac
     7081 tcaccaaaca gttcaccaaa tacgccttgg ctgacgtggc caagatggac agcacccacg
     7141 cgatcaggct ttacgagctg ctcatgcaat gggacagcat cggccagcgc gaaatagaaa
     7201 ttgaccagct gcgaaagtgg tttcaactgg aaggccggta tccctcgatc aaggacttca
     7261 agttgcgagt gcttgatcca gccgtgacgc agatcaacga gcacagcccg ctacaggtgg
     7321 agtgggcgca gcgaaagacc gggcgcaagg tcacacatct gttgttcagt tttggaccga
     7381 agaagcccgc caaggcggtg ggtaaggccc cagcgaagcg caaggccggg aagatttcag
     7441 atgctgagat cgcgaaacag gctcgccctg gtgagacatg ggaagcggcc cgcgctcgac
     7501 taacccagat gccgcttgcg caaactatta actggcgaac tacttactct agcttcccgg
     7561 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     7621 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg cgggactctg
     7681 gggttcgaga gctcgcttgg actcctgttg atagatccag taatgacctc agaactccat
     7741 ctggatttgt tcagaacgct cggttgccgc cgggcgtttt ttattggtga gaatccaagc
     7801 actagtcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     7861 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     7921 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     7981 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta
     8041 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     8101 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     8161 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     8221 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     8281 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     8341 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     8401 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     8461 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     8521 tgctcacatg ttctttcctg cgttgtcgac agagcccggg cggcatcaga gcagattgta
     8581 ctgagagtgc accataatcg gcattttctt ttgcgttttt atttgttaac tgttaattgt
     8641 ccttgttcaa ggatgctgtc tttgacaaca gatgttttct tgcctttgat gttcagcagg
     8701 aagctaggcg caaacgttga ttgtttgtct gcgtagaatc ctctgtttgt catatagctt
     8761 gtaatcacga cattgtttcc tttcgcttga ggtacagcga agtgtgagta agtaaaggtt
     8821 acatcgttag gatcaagatc catttttaac acaaggccag ttttgttcag cggcttgtat
     8881 gggccagtta aagaattaga aacataacca agcatgtaaa tatcgttaga cgtaatgccg
     8941 tcaatcgtca tttttgatcc gcgggagtca gtgaacagat accatttgcc gttcatttta
     9001 aagacgttcg cgcgttcaat ttcatctgtt actgtgttag atgcaatcag cggtttcatc
     9061 acttttttca gtgtgtaatc atcgtttagc tcaatcatac cgagagcgcc gtttgctaac
     9121 tcagccgtgc gttttttatc gctttgcaga agtttttgac tttcttgacg gaagaatgat
     9181 gtgcttttgc catagtatgc tttgttaaat aaagattctt cgccttggta gccatcttca
     9241 gttccagtgt ttgcttcaaa tactaagtat ttgtggcctt tatcttctac gtagtgagga
     9301 tctctcagcg tatggttgtc gcctgagctg tagttgcctt catcgatgaa ctgctgtaca
     9361 ttttgatacg tttttccgtc accgtcaaag attgatttat aatcctctac accgttgatg
     9421 ttcaaagagc tgtctgatgc tgatacgtta acttgtgcag ttgtcagtgt ttgtttgccg
     9481 taatgtttac cggagaaatc agtgtagaat aaacggattt ttccgtcaga tgtaaatgtg
     9541 gctgaacctg accattcttg tgtttggtct tttaggatag aatcatttgc atcgaatttg
     9601 tcgctgtctt taaagacgcg gccagcgttt ttccagctgt caatagaagt ttcgccgact
     9661 ttttgataga acatgtaaat cgatgtgtca tccgcatttt taggatctcc ggctaatgca
     9721 aagacgatgt ggtagccgtg atagtttgcg acagtgccgt cagcgttttg taatggccag
     9781 ctgtcccaaa cgtccaggcc ttttgcagaa gagatatttt taattgtgga cgaatcgaac
     9841 tcaggaactt gatatttttc atttttttgc tgttcaggga tttgcagcat atcatggcgt
     9901 gtaatatggg aaatgccgta tgtttcctta tatggctttt ggttcgtttc tttcgcaaac
     9961 gcttgagttg cgcctcctgc cagcagtgcg gtagtaaagg ttaatactgt tgcttgtttt
    10021 gcaaactttt tgatgttcat cgttcatgtc tcctttttta tgtactgtgt tagcggtctg
    10081 cttcttccag ccctcctgtt tgaagatggc aagttagtta cgcacaataa aaaaagacct
    10141 aaaatatgta aggggtgacg ccaaagtata cactttgccc tttacacatt ttaggtcttg
    10201 cctgctttat cagtaacaaa cccgcgcgat ttacttttcg acctcattct attagactct
    10261 cgtttggatt gcaactggtc tattttcctc ttttgtttga tagaaaatca taaaaggatt
    10321 tgcagactac gggcctaaag aactaaaaaa tctatctgtt tcttttcatt ctctgtattt
    10381 tttatagttt ctgttgcatg ggcataaagt tgccttaag
//
