LOCUS       sequence-217622-       10140 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  P element vector for transposon insertion of dhd-eGFP into 
            Drosophila genome.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10140)
  TITLE     Orr-Weaver plasmids
REFERENCE   2  (bases 1 to 10140)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCaSpeR dhd-eGFP
FEATURES             Location/Qualifiers
     source          1..10140
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    join(824..854,855..1409)
                     /label=P element 5' end
     CDS             complement(join(2188..2802,2873..3004,3208..3523,3585..4239,
                     4314..4587,4989..5060))
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     primer_bind     5512..5530
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     regulatory      6808..6817
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(6814..6816,6817..6819,6820..7533)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTSKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6859..6880)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7120..7139)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7467..7488
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     regulatory      7545..7554
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    join(7970..8171,8172..8202)
                     /label=P element 3' end
                     /note="P element 3' end"
     primer_bind     complement(8740..8759)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8859..8881
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8919..8937)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9005..9109
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9110..9178,9179..9970)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9328..9347)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccttctt
      601 cttgaactcg ggctcggtgc cagtatacct caaatggttg tcgtacctct catggttccg
      661 ttacgccaac gagggtctgc tgattaacca atgggcggac gtggagccgg gcgaaattag
      721 ctgcacatcg tcgaacacca cgtgccccag ttcgggcaag gtcatcctgg agacgcttaa
      781 cttctccgcc gccgatctgc cgctggacta cgtgggtctg gcccatgatg aaataacata
      841 aggtggtccc gtcgatagcc gaagcttacc gaagtataca cttaaattca gtgcacgttt
      901 gcttgttgag aggaaaggtt gtgtgcggac gaattttttt ttgaaaacat taacccttac
      961 gtggaataaa aaaaaatgaa atattgcaaa ttttgctgca aagctgtgac tggagtaaaa
     1021 ttaattcacg tgccgaagtg tgctattaag agaaaattgt gggagcagag ccttgggtgc
     1081 agccttggtg aaaactccca aatttgtgat acccacttta atgattcgca gtggaaggct
     1141 gcacctgcaa aaggtcagac atttaaaagg aggcgactca acgcagatgc cgtacctagt
     1201 aaagtgatag agcctgaacc agaaaagata aaagaaggct ataccagtgg gagtacacaa
     1261 acagagtaag tttgaatagt aaaaaaaatc atttatgtaa acaataacgt gactgtgcgt
     1321 taggtcctgt tcattgttta atgaaaataa gagcttgagg gaaaaaattc gtactttgga
     1381 gtacgaaatg cgtcgtttag agcagcagcc gaattaattc tagttccagt gaaatccaag
     1441 cattttctaa attaaatgta ttcttattat tatagttgtt atttttgata tatataaaca
     1501 acactattat gcccaccatt tttttgagat gcatctacac aaggaacaaa cactggatgt
     1561 cactttcagt tcaaattgta acgctaatca ctccgaacag gtcacaaaaa attaccttaa
     1621 aaagtcataa tattaaatta gaataaatat agctgtgagg gaaatatata caaatatatt
     1681 ggagcaaata aattgtacat acaaatattt attactaatt tctattgaga cgaaatgaac
     1741 cactcggaac catttgagcg aaccgaatcg cgcggaacta acgacagtcg ctccaaggtc
     1801 gtcgaacaaa aggtgaatgt gttgcggaga gcgggtggga gacagcgaaa gagcaactac
     1861 gaaacgtggt gtggtggagg tgaattatga agagggcgcg cgatttgaaa agtatgtata
     1921 taaaaaatat atcccggtgt tttatgtagc gataaacgag tttttgatgt aaggtatgca
     1981 ggtgtgtaag tcttttggtt agaagacaaa tccaaagtct acttgtgggg atgttcgaag
     2041 gggaaatact tgtattctat aggtcatatc ttgtttttat tggcacaaat ataattacat
     2101 tagctttttg agggggcaat aaacagtaaa cacgatggta ataatggtaa aaaaaaaaaa
     2161 caagcagtta tttcggatat atgtcggcta ctccttgcgt cgggcccgaa gtcttagagc
     2221 cagatatgcg agcacccgga agctcacgat gagaatggcc agacccacgt agtccagcgg
     2281 cagatcggcg gcggagaagt taagcgtctc caggatgacc ttgcccgaac tggggcacgt
     2341 ggtgttcgac gatgtgcagc taatttcgcc cggctccacg tccgcccatt ggttaatcag
     2401 cagaccctcg ttggcgtaac ggaaccatga gaggtacgac aaccatttga ggtatactgg
     2461 caccgagccc gagttcaaga agaagccgcc aaagagcagg aatggtatga taaccggcgg
     2521 acccacagac agcgccatcg aggtcgagga gctggcgcag gatattagat atccgaagga
     2581 cgttgacaca ttggccacca gagtgaccag cgccaggcag ttgaagaagt gcagcactcc
     2641 ggcccgcagt ccgatcatcg gataggcaat cgccgtgaag accagtggca ctgtgagaaa
     2701 aagcggcaat tcggcaatcg ttttgcccag aaagtatgtg tcacagcgat aaagtcgact
     2761 tcgggcctcc ctcataaaaa ctggcagctc tgaggtgaac acctaaatcg aatcgattca
     2821 ttagaaagtt agtaaattat tgaaatgcaa atgtattcta aacatgactt acatttatcg
     2881 tggcaaagac gttttgaaag gtcatgttgg tcaggaagag gaagatggct ccgttgatat
     2941 tcatcacacc cacttgcgtg agttgttggc ccaaaaagat gaggccaatc aagatggcaa
     3001 ccatctgcaa attaaaatgt tactcgcatc tcattaatat tcgcgagtta aatgaaattt
     3061 atttatcttc tgcaaaacta taaactatac atctcattga aaaaaactaa gaagggtgtg
     3121 gaatcaggca attctatcta aaatctagcg aatttgtttc caagaattgt aagcgttata
     3181 tcatttgttt ccactggaac cactcaccgt tgtctgaata agtcgcactt ttacgaggag
     3241 tggttccttg agcaccgaca gccaggatcg ccacaggacc gcccggaact gcatgaacca
     3301 ggtggccttg taggtgtacc cattctccgg ctgctccagt ggcttctcca gatttttggt
     3361 ggccaacaac tgctccatat cccgggctac tttgctaatg gcaaaattgt cgcatatctt
     3421 ggcgatccga tcacgggact cgatctcccg tccgggcaca acggccaaca cctgtacgta
     3481 aaagtccgcc ggattgtagt tggtaggaca ctgggcaccc acgctggata ggagttgaga
     3541 tgttatgtaa tactagatac ccttaataaa cacatcgaac tcactaggaa aagaagtcga
     3601 cggcttcgct gggagtgccc aagaaagcta ccctgccctc ggccatcaga aggatcttgt
     3661 caaagagctc aaacagctcg gaagacggct gatgaatggt caggatgacg gtcttgccct
     3721 tctgcgacag cttcttcagc acctggacga cgctgtgggc ggtaaaggag tccagtccgg
     3781 aggtgggctc atcgcagatc agaagcggcg gatcggttag agcctcggag gcgaatgcca
     3841 gacgcttcct ttctccgccg gacagacctt tcaccctgcc gggcacaccg atgatcgtgt
     3901 gctgacattt gctgagcgaa agctcctgga tcacctgatc cacgcgggcc actcgctgcc
     3961 gataggtcag atgtcgtggc atccgcacca tggcttggaa aatcaggtgt tccctggccg
     4021 ttagggagcc gataaagagg tcatcctgct ggacataggc gcacctggcc tgcatctcct
     4081 tggcgtccac aggttggcca ttgagcagtc gcatcccgga tggcgatact tggatgccct
     4141 gcggcgatcg aaaggcaagg gcattcagca gggtcgtctt tccggcaccg gaactgccca
     4201 tcacggccaa aagttcgccc ggataggcca cgccgcaaac tgagtttcaa attggtaatt
     4261 ggacccttta ttaagatttc acacagatca gccgactgcg aatagaaact caccgttctt
     4321 gagcaaatgt ttcctgggcg ccggtatgtg tcgctcgttg cagaatagtc cgcgtgtccg
     4381 gttgaccagc tgccgccatc cggagcccgg ctgattgacc gccccaaaga tgtccatatt
     4441 gtgccaggca taggtgaggt tctcggctag ttggccgctc cctgaaccgg agtcctccgg
     4501 cggactgggt ggccggagcg tgccgtagtt tttggcctgc ccgaagccct ggttaatgca
     4561 gctctgcgaa gccgctccgc tgtcaccctg caatgatagg ggatctcaaa tatcaactac
     4621 aagcgttatg ctcatctaac cccgaacaaa aagtaccccg aagtatccta cgaagtaggt
     4681 ttatactttt atttattttt tgtgcatcta ggatcagctt aaaatatctg gttgttatat
     4741 tttttgtaaa aaagaatata gtcgaaaatg aatgccttta gatgtcttga tcatgatatg
     4801 atctcaaaaa ttgtcttata tagcgagaac agctaccaga ataatctgtt tcgtgtcact
     4861 atttgtttgt gcaattgcgg tttgggattt ttgtgggtcg cagttctcac gccgcagaca
     4921 atttgatgtt gcaatcgcag ttcctataga tcaagtgaac ttaagatgta tgcacatgta
     4981 ctactcacat tgttcagatg ctcggcagat gggtgtttgc tgcctccgcg aattaatagc
     5041 tcctgatcct cttggcccat tgccgggatt tttcacactt tcccctgctt acccacccaa
     5101 aaccaatcac caccccaatc actcaaaaaa caaacaaaaa taagaagcga gaggagtttt
     5161 ggcacagcac tttgtgttta attgatggcg taaaccgctt ggagcttcgt cacgaaaccg
     5221 ctgacaaaat gcaactgaag gcggacattg acgctacgta acgctacaaa cggtggcgaa
     5281 agagatagcg gacgcagcgg cgaaagagac ggcgatattt ctgtggacag agaaggaggc
     5341 aaacagcgct gactttgagt ggaatgtcat tttgagtgag aggtaatcga aagaacctgg
     5401 tacatcaaat acccttggat cgaagtaaat ttaaaactga tcagataagt tcaatgatat
     5461 ccagtgcagt aaaaaaaaaa aatgtttttt ttatctactt tccgcaaaaa tgggttttat
     5521 taacttacat acatactaga attcggtacc cgcccgggga tcagatccgc ggccgcatag
     5581 gccactagtc agttcgtcca gcttgggtgc tattatcttg caaggtccgc accagtctgc
     5641 ataaaaatca attaccacca gcttgtcctc ggcgaggatt agctgctggt caagatcgtc
     5701 cttgttccgc actgggtaca ccatgacttt aactaaattc ccaacggatt gcaaatcgaa
     5761 acgaaacaaa aaaaaaaaaa atgggaagag aagaaagaaa aaaaaaagga aaatgttaga
     5821 aaatcttgtt ttgggtgtaa aattcacttg atttgttttg ttacttttga attttaatgg
     5881 agctctgccc tcgccgagat atcagcccga aatgaaaatt gtagggcggc aggcgttctt
     5941 ttatacagtt ttcgccaaat attttgcggc cttcttggat tgaaaaaaaa gtgttttcaa
     6001 atgtcagcgg cgcggtgtgt tgaggtgagt ttggcttcga acctaccacg gctatggcaa
     6061 cactcgagga aaatattagt actatgtcgt gtgacgccat tattttgcca taattacgat
     6121 aataatattc atatcgatat cgatagacct gccgtggtga ataagtatgt tccccacccc
     6181 ccaacacact cggttggctt tcccaacgac ctggcaaccc taacacgccc gccattttgc
     6241 actctccatg ggccgccatt ttgtttagcg gccacgcgag aattcagcat gcaaattggc
     6301 gggaattcca ctctacacac cgccacttgg agacccctat aaagtcgccc acgcagccgc
     6361 catttcaatc agttcacaat ttgcagcgaa agcgccaaca gccccatcac gtcaagttct
     6421 ttaatttaaa aaaaagaaga aaaacacttt gctaatcctc gcgacataaa aatggcatcc
     6481 gtacgcacca tgaacgacta tcacaagcgc atcgaggcgg ccgacgacaa gctaatcgtg
     6541 ctggatttct atgcgacatg gtgtggtccc tgcaaggaaa tggagagcac cgtcaaatcg
     6601 ctggccagaa aatactccag caaggcggtg gtgctcaaga tcgatgtgga caaattcgag
     6661 gagctgacgg agcgctacaa ggtgcgcagc atgccaacgt ttgtcttttt gcgccaaaat
     6721 cgacgcctgg cctcctttgc cggcgccgac gagcacaagc tgaccaacat gatggccaag
     6781 ctggtgaagg cgggcggaag tggagcggcc gccatggtga gcaagggcga ggagctgttc
     6841 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
     6901 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc
     6961 accaccagca agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg
     7021 cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
     7081 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
     7141 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
     7201 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac
     7261 aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc
     7321 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc
     7381 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc
     7441 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
     7501 atcactctcg gcatggacga gctgtacaag taaagccctt aggcccatgg cggccgggag
     7561 catgcgacgt cgggcccgca gcaatcctgc ccagcaatta gcaatcagca gcgcatcttt
     7621 ttttttttta cttatttaac tcatctttta aacatgttcg cctcatttgt gttcgtttat
     7681 gtattcgatg ttatgtgtat gctcatgtga tgtttagctt gtaagcgcga gatgtgggta
     7741 gcaggagatg cagtgcagcc aacagcagtg accagatgat atatgctatg ctactactac
     7801 tacttatatg ctatgatttg tggcgcggag gcgtgtctgc gacacataat cccgcccatt
     7861 agctttaaga ttcaggcact aagaagcaat tcgatcaata aattattgta accactctgg
     7921 acatttaaat gttaactcga ggcctcgagg tcgacctgca gccaagcttt gcgtactcgc
     7981 aaattattaa aaataaaact ttaaaaataa tttcgtctaa ttaatattat gagttaattc
     8041 aaaccccacg gacatgctaa gggttaatca acaatcatat cgctgtctca ctcagactca
     8101 atacgacact cagaatacta ttcctttcac tcgcacttat tgcaagcata cgttaagtgg
     8161 atgtctcttg ccgacgggac caccttatgt tatttcatca tggtctggcc attctcatcg
     8221 tgagcttccg ggtgctcgca tatctggctc taagacttcg ggcccgacgc aaggagtagc
     8281 cgacatatat ccgaaataac tgcttgtttt ttttttttac cattattacc atcgtgttta
     8341 ctgtttattg ccccctcaaa aagctaatgt aattatattt gtgccaataa aaacaagata
     8401 tgacctatag aatacaagta tttccccttc gaacatcccc acaagtagac tttggatttg
     8461 tcttctaacc aaaagactta cacacctgca taccttacat caaaaactcg tttatcgcta
     8521 cataaaacac cgggatatat tttttatata catacttttc aaatcgcgcg ccctcttcat
     8581 aattcacctc caccacacca cgtttcgtag ttgctctttc gctgtctccc acccgctctc
     8641 cgcaacacat tcaccttttg ttcgacgacc ttggagcgac tgtcgttagt tccgcgcgat
     8701 tcggtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc
     8761 atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
     8821 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
     8881 gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt
     8941 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa
     9001 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     9061 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     9121 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     9181 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     9241 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     9301 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     9361 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     9421 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     9481 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     9541 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     9601 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     9661 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     9721 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     9781 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     9841 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     9901 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     9961 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
    10021 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
    10081 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
//
