LOCUS       sequence-217625-        3184 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pcUBI promoter module Pro+5U.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3184)
  TITLE     Chevalier Lab plasmids
REFERENCE   2  (bases 1 to 3184)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pORFE0011
FEATURES             Location/Qualifiers
     source          1..3184
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(406..424)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        492..596
                     /gene="bla"
                     /label=AmpR promoter
     CDS             818..1609
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1780..2368
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2269..2288
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2522..2539
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cgaaatcgtt gatttttttc atttaatcgt gtagctaatt gtacgtatac atatggatct
       61 acgtatcaat tgttcatctg tttgtgtttg tatgtataca gatctgaaaa catcacttct
      121 ctcatctgat tgtgttgtta catacataga tatagatctg ttatatcatt ttttttatta
      181 attgtgtata tatatatgtg catagatctg gattacatga ttgtgattat ttacatgatt
      241 ttgttattta cgtatgtata tatgtagatc tggacttttt ggagttgttg acttgattgt
      301 atttgtgtgt gtatatgtgt gttctgatct tgatatgtta tgtatgtgca gcgaattcgg
      361 cgcgcaaatg tgagaccacg aagtggctct tcagtggacg aaagggcctc gtgatacgcc
      421 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
      481 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
      541 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatgc
      601 gctcacgcaa ctggtccaga accttgaccg aacgcagcgg tggtaacggc gcagtggcgg
      661 ttttcatggc ttgttatgac tgtttttttt tggggtacag tctatgcctc gggcatccaa
      721 gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta tggagcagca acgatgttac
      781 gcagcagggc agtcgcccta aaacaaagtt aaacatcatg agggaagcgg tgatcgccga
      841 agtatcgact caactatcag aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt
      901 gctggccgta catttgtacg gctccgcagt ggatggcggc ctgaagccac acagcgatat
      961 tgatttgctg gttacggtga ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa
     1021 cgaccttttg gaaacttcgg cttcccctgg agagagcgag attctccgcg ctgtagaagt
     1081 caccattgtt gtgcacgacg acatcattcc gtggcgttat ccagctaagc gcgaactgca
     1141 atttggagaa tggcagcgca atgacattct tgcaggtatc ttcgagccag ccacgatcga
     1201 cattgatctg gctatcttgc tgacaaaagc aagagaacat agcgttgcct tggtaggtcc
     1261 agcggcggag gaactctttg atccggttcc tgaacaggat ctatttgagg cgctaaatga
     1321 aaccttaacg ctatggaact cgccgcccga ctgggctggc gatgagcgaa atgtagtgct
     1381 tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc
     1441 tgccgactgg gcaatggagc gcctgccggc ccagtatcag cccgtcatac ttgaagctag
     1501 acaggcttat cttggacaag aagaagatcg cttggcctcg cgcgcagatc agttggaaga
     1561 atttgtccat tacgtaaaag gcgagatcac caaggtagtc ggcaaataac tgtcagacca
     1621 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     1681 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     1741 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     1801 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     1861 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     1921 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     1981 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     2041 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     2101 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     2161 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     2221 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     2281 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     2341 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     2401 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     2461 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     2521 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc
     2581 gcgttggccg attcattaat cactctgtgg tctcaggagc tagcaacgat tgtacaattg
     2641 cttctttaaa aaaggaagaa agaaagaaag aaaagaatca acatcagcgt taacaaacgg
     2701 ccccgttacg gcccaaacgg tcatatagag taacggcgtt aagcgttgaa agactcctat
     2761 cgaaatacgt aaccgcaaac gtgtcatagt cagatcccct cttccttcac cgcctcaaac
     2821 acaaaaataa tcttctacag cctatatata caaccccccc ttctatctct cctttctcac
     2881 aattcatcat ctttctttct ctacccccaa ttttaagaaa tcctctcttc tcctcttcat
     2941 tttcaaggta aatctctctc tctctctctc tctctgttat tccttgtttt aattaggtat
     3001 gtattattgc tagtttgtta atctgcttat cttatgtatg ccttatgtga atatctttat
     3061 cttgttcatc tcatccgttt agaagctata aatttgttga tttgactgtg tatctacacg
     3121 tggttatgtt tatatctaat cagatatgaa tttcttcata ttgttgcgtt tgtgtgtacc
     3181 aatc
//
