LOCUS       sequence-21763-e         640 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 640)
  AUTHORS   Boehm JS, Zhao JJ, Yao J, Kim SY, Firestein R, Dunn IF, Sjostrom SK,
            Garraway LA, Weremowicz S, Richardson AL, Greulich H, Stewart CJ, 
            Mulvey LA, Shen RR, Ambrogio L, Hirozane-Kishikawa T, Hill DE, Vidal
            M, Meyerson M, Grenier JK, Hinkle G, Root DE, Roberts TM, Lander ES,
            Polyak K, Hahn WC
  TITLE     Integrative Genomic Approaches Identify IKBKE as a Breast Cancer 
            Oncogene.
  JOURNAL   Cell. 2007 Jun 15. 129(6):1065-1079.
  PUBMED    17574021
REFERENCE   2  (bases 1 to 640)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2007 
            Jun 15. 129(6):1065-1079."
COMMENT     Sequence Label: pBabe-Puro-Myr-Flag-IKBKE
FEATURES             Location/Qualifiers
     source          1..640
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             62..85
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    92..116
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ggtcttcaaa tctaaaccaa aggaccccag ccagcgccgg cgcaggatcc gaggttacct
       61 tgactacaaa gacgatgacg acaagcaatt gacaagtttg tacaaaaaag ttggcatgca
      121 gagcacagcc aattacctgt ggcacacaga tgacctgctg gggcaggggg ccactgccag
      181 tgtgtacaag gcccgcaaca agaaatccgg agagctggtt gctgtgaagg tcttcaacac
      241 taccagctac ctgcggcccc gcgaggtgca ggtgagggag tttgaggtcc tgcggaagct
      301 gaaccaccag aacatcgtca agctctttgc ggtggaggag acgggcggaa gccggcagaa
      361 ggtactggtg atggagtact gctccagtgg gagcctgctg agtgtgctgg agagccctga
      421 gaatgccttt gggctgcctg aggatgagtt cctggtggtg ctgcgctgtg tggtggccgg
      481 catgaaccac ctgcgggaga acggcattgt gcatcgcgac atcaagccgg ggaacatcat
      541 gcgcctcgta ggggaggagg ggcagagcat ctacaagctg acagacttcg gcgctgcccg
      601 ggacctggat gatgatgaga aattcgtctc ggtctatggg
//
