LOCUS       sequence-217638-        8689 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  prp16-2 in pSE358(Trp).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8689)
  AUTHORS   Madhani HD, Guthrie C
  TITLE     Genetic interactions between the yeast RNA helicase homolog Prp16 
            and spliceosomal snRNAs identify candidate ligands for the Prp16 
            RNA-dependent ATPase.
  JOURNAL   Genetics. 1994 Jul;137(3):677-87.
  PUBMED    8088513
REFERENCE   2  (bases 1 to 8689)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics.";
            date: "1994-07"; volume: "137(3)"; pages: "677-87"
COMMENT     Sequence Label: HM110 prp16-2
FEATURES             Location/Qualifiers
     source          1..8689
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    110..131
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(146..151,152..169,170..176)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    184..200
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     189..211
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     208..224
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     208..224
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4792..4809)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4792..4808)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4801..4823)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    5323..5615
                     /label=CEN4
                     /note="S. cerevisiae CEN4 centromere"
     rep_origin      5616..6069
                     /label=ARS1
                     /note="S. cerevisiae autonomously replicating sequence 
                     ARS1"
     CDS             complement(5908..6582)
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     promoter        complement(6583..6684)
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     primer_bind     complement(6701..6719)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6787..6891
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6892..6960,6961..7752)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7110..7129)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7923..8511
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8412..8431
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8665..8682
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
       61 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
      121 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
      181 gaattgtgag cggataacaa tttcacacag gaaacagcta tgacatgatt acgaattcga
      241 gctcggtacc cgggctccca ttagcagcag caattggctt ctttggtgtc ttcttcttct
      301 tcaaagtttt cttcaattcc gccagcgtgc tttccaaatc gaaattgttg tcaaacaaag
      361 ctagcttaag tgaaagatta tcccagcctt ggtaatcctg taactgcgcc tttagagtag
      421 gaaatacctc gttcattaga tcatattcat cgtcattcaa ataatcatca aattcacctt
      481 cgtcgtgaaa gtccggcatg tcgtctgctc catcgctgta gtcactgtaa gccatctcga
      541 tagtcaatag ttgtcgaagc agaagccttt gggtgtctcc aagctgtact taactctttt
      601 taaattgctt attggttagc tttcatgtgg tatattcaag tcacattaat gggcgtctcc
      661 gtgcattgtg atcaatatat gaatacgaag tacttataga gtagtattta cacgattgtt
      721 attatattta tatagcattc acgcctggta ccacaggagt tttctacgct tacggtcctc
      781 tctggctata cgcctcttct ccttccagcg tgattggatt tcttgcaatc tactttccat
      841 aatttgtaca tgttgttcac taggtttcga gaccatggat acaaataatg gcgaaatgcc
      901 atattttgca gccaaaaatt tgcgtgtata cttttgagga ttgtcaagac gtaacttccg
      961 gatctcatca atttcttgag gtcccagatg ataggttttg ccgttcactg tgctcttacc
     1021 tacgagaaca tttggacact gcttcgcttt agtgctttga acaggcatgt caagccctct
     1081 acgagggtca ctggctgcca taaagctacg tggaatggtc tcactgttaa cagatccggt
     1141 tgcgatggat tctgatggtt gatagtacat tcctggagca tctctgtgct ttaaataccc
     1201 tttatagtta gatgcgctct tagtcttgtt gtaaatcgtt gtcacttgtg ttttgggaaa
     1261 tccaaattgc ttccaggcag atccagcagt gtggaacgat ctgcagcaca cacctctgcc
     1321 aatcattctt gtgctatttt actttgtctt taattttcca ttttaacttg ttgaaagtat
     1381 ctctttttgc cctcttttct attttctcaa ataataaaag cggtgaaagc tgaaaaattc
     1441 atgagatgac tgcagatgat aaaacacaag aaggcccaaa gacaatacaa atacctctgg
     1501 tatgggtcat tcggggcgtg aggaaaggat aaaagacata tttaaggaac ttacgtcaaa
     1561 agaactaacg cctgggttgc ttttgacact gcagaaacta gcgcaaaagc cgaatactaa
     1621 tctagaacag tttattgcca gctgtaaagc tttgacgaaa ctcagcagta acaatccaat
     1681 catttttaat gaacttctgg agctcctcaa gaataaatct gaagaggact cgacaggccc
     1741 aaagaaaatc gccccttcta taaataagag aaagaaattt aagatccagt tagaccttga
     1801 cgataatgaa gatgagctag attctccagt tcaaaaaaaa ccagcaccta cacggacgtt
     1861 gttcaagaga atagataaac taaaggccaa acagttgagg cagtattcgc ccaccgtgaa
     1921 ggatccttct ccaaatagtg aacagcaaac acagaatggt catgctgaaa cgaaggacta
     1981 tgaaccgact cgaagcgaag tagttgagga ggacagagaa tggtatgata atgatgatga
     2041 ttatggaaat ttggtgcctg aacctctgtc tgaattgcct gaggaagcga aactactacc
     2101 tgtcattagg aatatcgaca acgatgatgc cttacgaaat accgttcaat tataccccat
     2161 accactcaag caaagaatgg aatggatccc tcctttttta tcaaagtttg ctcttgaaaa
     2221 caaagtgccc acttccataa ttatcgggtc catctctgag acgtccagcc aagtatccgc
     2281 tttatcaatg gtgaatccat ttaggaatcc tgacagtgaa ttttctgcga acgccaaacg
     2341 aggtagtaag ctggttgcct tgagaaggat aaacatggaa catattcaac aatctcgaga
     2401 caacaccact gttttgaaca ctgccatggg ggaagtacta ggcttggaga ataacaataa
     2461 agcaaaagat aagagcaacc aaaagatttg cgacgataca gctcttttca cgccatcaaa
     2521 agatgacatt aaacatacta aagagcaact gcctgttttc cgctgcagat ctcaattgtt
     2581 atcattgata agagaaaatc aagtagtggt gataattggt gaaacgggct caggtaaaac
     2641 cacgcaactt gcacagtatt tatatgaaga aggatatgcc aacgataggg ggaaatctat
     2701 tgttgtcaca cagccgagaa gagtagcagc catatccgtt gcaaaaaggg ttgcaatgga
     2761 aatgcaagtt ccattgggta aggaagtagg ctattctata aggtttgaag atgtgacgga
     2821 ttctgagtgt acaaaactta agtttgtaac ggatggtatt ctactcagag agaccttatt
     2881 ggatgataca ctcgataaat attcgtgtgt tattattgat gaagctcatg aaaggtcatt
     2941 aaatacagac attttactag gttttttcaa gattttatta gcacgtagaa gagatctaaa
     3001 actcataatc acttcagcaa caatgaatgc gaagaagttc tccgcatttt ttggtaatgc
     3061 tcctcagttc acaattcctg gaagaacttt ccccgtacag accatatata cttccaaccc
     3121 tgttcaagac tacgtagaag ctgcagtttc tcaggctgtg aaaattcatt tagcaaacga
     3181 ttgttcaagc ggtgatattc tcattttcat gacaggtcaa gaaaatattg aaacaacgtt
     3241 cgacactctg caagaaaagt ttttgcaagt atattctaaa aagtttggta ccgcaaactt
     3301 tgaagagatc aatgatattg aaattttacc aatctactcg gctttacctg cagatttgca
     3361 attcaaaatt tttcaggatc tacatggcac taaaaggaaa attataattg cgacaaatat
     3421 tgcagagact tcattgacca ttaaaggtat tagatatgtc attgattgtg gttactctaa
     3481 actgaaagtt tacaatccaa agattgggct tgatagttta gtaataacgc ccatatcaaa
     3541 agctaacgcc gaccaaagat ccggaagagc gggaagaact gcgccaggca ctgcatatcg
     3601 attatacacg gaagatacat tcaaagaaga tatgtactta cagacaatac ctgagatcca
     3661 aagaacgaat ttgtcaaaca cccttttact gctaaaatct ctggacgtta ctgacgaatt
     3721 gagtaagttc ccgttcattg ataaaccacc cttacaaaca tttttatcct cattgtatga
     3781 gttatggttc ataggggcta tagacactag tggacaattg actccattgg gattacagat
     3841 ggcaaaattt cccctgcaac cttccctttc caaaatttta ttaattgcag ttagaaacgg
     3901 ctgcagtgat gaaatgttaa caatagtttc catgctttct gttccacaag tcttttatag
     3961 acccaaggaa agacagaaag aagcagatat tgctagaaat aaatttttta tcgcgaaatc
     4021 agatcactta acgttactta atgtttttga acaatggagg gcaaataatt ttagttcgca
     4081 ttggtgtaac aaacattttg ttcaatacaa atcacttgtc agggcaagag acattcgaga
     4141 ccagttgcta acaatactaa aatctcaaaa aattccggtc atttcctccg gtaaggactg
     4201 ggatattatt aaaaaatgta tttgttcagg atttgctcat caagcagcca aaataacagg
     4261 actacgaaat tacgttcatt taaaaactgg cgtaagtgtt caactacacc ctacaagtgc
     4321 attacacgga ttgggggatt taccccctta cgtggtatat catgaattat taatgactag
     4381 caaagaatac atttgttgtg ttacttctgt agatccattc tggttaatgg aatatggtgg
     4441 gttactctac gacatcaagc gtattaaaaa tgatcaggag gccacaacta caggcctctt
     4501 tggtgaacac tatgaacata ctctggacaa ggttgaagat gatattgata tcaatattag
     4561 gagatgtaag gatatgagag acagtgtgat acaggagctc aaaatgaccg acaattcaaa
     4621 caaagaagac aaaaagcaaa aaacgaaaaa gcaaaatata ctgaacggca aagaaaattc
     4681 aatgaaacct ttcaaaagaa ggaagccttt tttttagttt atgggaagag cgtgctaata
     4741 ggcaaaatat tcatatatgt tattatatag ttatatgcat gcaagcttgg cactggccgt
     4801 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
     4861 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
     4921 acagttgcgc agcctgaatg gcgaatggcg cctgatgcgg tattttctcc ttacgcatct
     4981 gtgcggtatt tcacaccgca taactagctt ttaacttgta tcctaggtta tctatgctgt
     5041 ctcaccatag agaatattac ctatttcaga atgtatgtcc atgattcgcc gggtaaatac
     5101 atataataca caaatctggc ttaataaagt ctataatata tctcataaag aagtgctaaa
     5161 ttggctagtg ctatatattt ttaagaaaat ttcttttgac taagtccata tcgactttgt
     5221 aaaagttcac attagcatac atatattaca cgagccagaa atagtaactt ttgcctaaat
     5281 cacaaattgc aaaatttaat tgcttgcaaa aggtcacatg cttataatca acttttttaa
     5341 aaatttaaaa tactttttta ttttttattt ttaaacataa atgaaataat ttatttattg
     5401 tttatgatta ccgaaacata aaacctgctc aagaaaaaga aactgttttg tccttggaaa
     5461 aaaagcacta cctaggagcg gccaaaatgc cgaggctttc atagcttaaa ctctttacag
     5521 aaaataggca ttatagatca gttcgagttt tcttattctt ccttccggtt ttatcgtcac
     5581 agttttacag taaataagta tcacctctta gagttggcgg ttgtttgcaa gaccgagaaa
     5641 aggctagcaa gaatcgggtc attgtagcgt atgcgcctgt gaacattctc ttcaacaagt
     5701 ttgattccat tgcggtgaaa tggtaaaagt caaccccctg cgatgtatat tttcctgtac
     5761 aatcaatcaa aaagccaaat gatttagcat tatctttaca tcttgttatt ttacagattt
     5821 tatgtttaga tcttttatgc ttgcttttca aaaggcctgc aggcaagtgc acaaacaata
     5881 cttaaataaa tactactcag taataaccta tttcttagca tttttgacga aatttgctat
     5941 tttgttagag tcttttacac catttgtctc cacacctccg cttacatcaa caccaataac
     6001 gccatttaat ctaagcgcat caccaacatt ttctggcgtc agtccaccag ctaacataaa
     6061 atgtaagctt tcggggctct cttgccttcc aacccagtca gaaatcgagt tccaatccaa
     6121 aagttcacct gtcccacctg cttctgaatc aaacaaggga ataaacgaat gaggtttctg
     6181 tgaagctgca ctgagtagta tgttgcagtc ttttggaaat acgagtcttt taataactgg
     6241 caaaccgagg aactcttggt attcttgcca cgactcatct ccatgcagtt ggacgatatc
     6301 aatgccgtaa tcattgacca gagccaaaac atcctcctta ggttgattac gaaacacgcc
     6361 aaccaagtat ttcggagtgc ctgaactatt tttatatgct tttacaagac ttgaaatttt
     6421 ccttgcaata accgggtcaa ttgttctctt tctattgggc acacatataa tacccagcaa
     6481 gtcagcatcg gaatctagag cacattctgc ggcctctgtg ctctgcaagc cgcaaacttt
     6541 caccaatgga ccagaactac ctgtgaaatt aataacagac atactccaag ctgcctttgt
     6601 gtgcttaatc acgtatactc acgtgctcaa tagtcaccaa tgccctccct cttggccctc
     6661 tccttttctt ttttcgaccg aatttcttga agacgaaagg gcctcgtgat acgcctattt
     6721 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
     6781 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     6841 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     6901 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     6961 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     7021 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     7081 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac
     7141 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     7201 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     7261 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     7321 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     7381 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     7441 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     7501 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     7561 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     7621 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     7681 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     7741 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     7801 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     7861 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     7921 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     7981 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     8041 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     8101 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     8161 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     8221 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     8281 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     8341 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     8401 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     8461 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     8521 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     8581 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     8641 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgg
//
