LOCUS       sequence-21764-e         881 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 881)
  AUTHORS   Boehm JS, Zhao JJ, Yao J, Kim SY, Firestein R, Dunn IF, Sjostrom SK,
            Garraway LA, Weremowicz S, Richardson AL, Greulich H, Stewart CJ, 
            Mulvey LA, Shen RR, Ambrogio L, Hirozane-Kishikawa T, Hill DE, Vidal
            M, Meyerson M, Grenier JK, Hinkle G, Root DE, Roberts TM, Lander ES,
            Polyak K, Hahn WC
  TITLE     Integrative Genomic Approaches Identify IKBKE as a Breast Cancer 
            Oncogene.
  JOURNAL   Cell. 2007 Jun 15. 129(6):1065-1079.
  PUBMED    17574021
REFERENCE   2  (bases 1 to 881)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2007 
            Jun 15. 129(6):1065-1079."
COMMENT     Sequence Label: pBabe-Puro-Myr-Flag-AKT1
FEATURES             Location/Qualifiers
     source          1..881
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..39
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="GSSKSKPKDPSQR"
     CDS             64..87
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    94..118
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gggtcttcaa aatctaaacc aaaggacccc agccagcgcc ggcgcaggat ccgaggttac
       61 cttgactaca aagacgatga cgacaagcaa ttgacaagtt tgtacaaaaa agttggcatg
      121 agcgacgtgg ctattgtgaa ggagggttgg ctgcacaaac gaggggagta catcaagacc
      181 tggcggccac gctacttcct cctcaagaat gatggcacct tcattggcta caaggagcgg
      241 ccgcaggatg tggaccaacg tgaggctccc ctcaacaact tctctgtggc gcagtgccag
      301 ctgatgaaga cggagcggcc ccggcccaac accttcatca tccgctgcct gcagtggacc
      361 actgtcatcg aacgcacctt ccatgtggag actcctgagg agcgggagga gtggacaacc
      421 gccatccaga ctgtggctga cggcctcaag aagcaggagg aggaggagat ggacttccgg
      481 tcgggctcac ccagtgacaa ctcaggggct gaagagatgg aggtgtccct ggccaagccc
      541 aagcaccgcg tgaccatgaa cgagtttgag tacctgaagc tgctgggcaa gggcactttc
      601 ggcaaggtga tcctggtgaa ggagaaggcc acaggccgct actacgccat gaagatcctc
      661 aagaaggaag tcatcgtggc caaggacgag gtggcccaca cactcaccga gaaccgcgtc
      721 ctgcagaact ccaggcaccc cttcctcaca gccctgaagt actctttcca gacccacgac
      781 cgcctctgct ttgtcatgga gtacgccaac gggggcgagc tgttcttcca cctgtcccgg
      841 gagcgtgtgt tctccgagga ccgggcccgc ttctatggcg c
//
