LOCUS       sequence-217643-        2946 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  tagRFP module CT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2946)
  TITLE     Chevalier Lab plasmids
REFERENCE   2  (bases 1 to 2946)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pJPN0016
FEATURES             Location/Qualifiers
     source          1..2946
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(712..730)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        798..902
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1124..1915
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2086..2674
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2575..2594
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2828..2845
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             join(2926..2946,1..672)
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="SELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIKVV
                     EGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTAT
                     QDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSDMALKL
                     VGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAVARYCD
                     LPSKLGHK"
ORIGIN
        1 atgcacatga agctgtacat ggagggcacc gtgaacaacc accacttcaa gtgcacatcc
       61 gagggcgaag gcaagcccta cgagggcacc cagaccatga gaatcaaggt ggtcgagggc
      121 ggccctctcc ccttcgcctt cgacatcctg gctaccagct tcatgtacgg cagcagaacc
      181 ttcatcaacc acacccaggg catccccgac ttctttaagc agtccttccc tgagggcttc
      241 acatgggaga gagtcaccac atacgaagat gggggcgtgc tgaccgctac ccaggacacc
      301 agcctccagg acggctgcct catctacaac gtcaagatca gaggggtgaa cttcccatcc
      361 aacggccctg tgatgcagaa gaaaacactc ggctgggagg ccaacaccga gatgctgtac
      421 cccgctgacg gcggcctgga aggcagaagc gacatggccc tgaagctcgt gggcgggggc
      481 cacctgatct gcaacttcaa gaccacatac agatccaaga aacccgctaa gaacctcaag
      541 atgcccggcg tctactatgt ggaccacaga ctggaaagaa tcaaggaggc cgacaaagaa
      601 acctacgtcg agcagcacga ggtggctgtg gccagatact gcgacctccc tagcaaactg
      661 gggcacaagt gagctttgag accacgaagt ggctcttcag tggacgaaag ggcctcgtga
      721 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca
      781 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
      841 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
      901 gtatgcgctc acgcaactgg tccagaacct tgaccgaacg cagcggtggt aacggcgcag
      961 tggcggtttt catggcttgt tatgactgtt tttttttggg gtacagtcta tgcctcgggc
     1021 atccaagcag caagcgcgtt acgccgtggg tcgatgtttg atgttatgga gcagcaacga
     1081 tgttacgcag cagggcagtc gccctaaaac aaagttaaac atcatgaggg aagcggtgat
     1141 cgccgaagta tcgactcaac tatcagaggt agttggcgtc atcgagcgcc atctcgaacc
     1201 gacgttgctg gccgtacatt tgtacggctc cgcagtggat ggcggcctga agccacacag
     1261 cgatattgat ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt
     1321 gatcaacgac cttttggaaa cttcggcttc ccctggagag agcgagattc tccgcgctgt
     1381 agaagtcacc attgttgtgc acgacgacat cattccgtgg cgttatccag ctaagcgcga
     1441 actgcaattt ggagaatggc agcgcaatga cattcttgca ggtatcttcg agccagccac
     1501 gatcgacatt gatctggcta tcttgctgac aaaagcaaga gaacatagcg ttgccttggt
     1561 aggtccagcg gcggaggaac tctttgatcc ggttcctgaa caggatctat ttgaggcgct
     1621 aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt
     1681 agtgcttacg ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga
     1741 tgtcgctgcc gactgggcaa tggagcgcct gccggcccag tatcagcccg tcatacttga
     1801 agctagacag gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt
     1861 ggaagaattt gtccattacg taaaaggcga gatcaccaag gtagtcggca aataactgtc
     1921 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     1981 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     2041 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     2101 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     2161 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     2221 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     2281 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     2341 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     2401 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     2461 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     2521 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     2581 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     2641 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     2701 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     2761 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     2821 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct
     2881 ccccgcgcgt tggccgattc attaatcact ctgtggtctc attcgagcga gctgattaag
     2941 gagaac
//
