LOCUS       sequence-217647-        2942 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  tagRFP module NT2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2942)
  TITLE     Chevalier Lab plasmids
REFERENCE   2  (bases 1 to 2942)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pJPN0015
FEATURES             Location/Qualifiers
     source          1..2942
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..690
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSDMALK
                     LVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAVARYC
                     DLPSKLGH"
     primer_bind     complement(732..750)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        818..922
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1144..1935
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2106..2694
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2595..2614
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2848..2865
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 atgagcgagc tgattaagga gaacatgcac atgaagctgt acatggaggg caccgtgaac
       61 aaccaccact tcaagtgcac atccgagggc gaaggcaagc cctacgaggg cacccagacc
      121 atgagaatca aggtggtcga gggcggccct ctccccttcg ccttcgacat cctggctacc
      181 agcttcatgt acggcagcag aaccttcatc aaccacaccc agggcatccc cgacttcttt
      241 aagcagtcct tccctgaggg cttcacatgg gagagagtca ccacatacga agatgggggc
      301 gtgctgaccg ctacccagga caccagcctc caggacggct gcctcatcta caacgtcaag
      361 atcagagggg tgaacttccc atccaacggc cctgtgatgc agaagaaaac actcggctgg
      421 gaggccaaca ccgagatgct gtaccccgct gacggcggcc tggaaggcag aagcgacatg
      481 gccctgaagc tcgtgggcgg gggccacctg atctgcaact tcaagaccac atacagatcc
      541 aagaaacccg ctaagaacct caagatgccc ggcgtctact atgtggacca cagactggaa
      601 agaatcaagg aggccgacaa agaaacctac gtcgagcagc acgaggtggc tgtggccaga
      661 tactgcgacc tccctagcaa actggggcac aaaggttgag accacgaagt ggctcttcag
      721 tggacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
      781 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
      841 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
      901 ataatattga aaaaggaaga gtatgcgctc acgcaactgg tccagaacct tgaccgaacg
      961 cagcggtggt aacggcgcag tggcggtttt catggcttgt tatgactgtt tttttttggg
     1021 gtacagtcta tgcctcgggc atccaagcag caagcgcgtt acgccgtggg tcgatgtttg
     1081 atgttatgga gcagcaacga tgttacgcag cagggcagtc gccctaaaac aaagttaaac
     1141 atcatgaggg aagcggtgat cgccgaagta tcgactcaac tatcagaggt agttggcgtc
     1201 atcgagcgcc atctcgaacc gacgttgctg gccgtacatt tgtacggctc cgcagtggat
     1261 ggcggcctga agccacacag cgatattgat ttgctggtta cggtgaccgt aaggcttgat
     1321 gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa cttcggcttc ccctggagag
     1381 agcgagattc tccgcgctgt agaagtcacc attgttgtgc acgacgacat cattccgtgg
     1441 cgttatccag ctaagcgcga actgcaattt ggagaatggc agcgcaatga cattcttgca
     1501 ggtatcttcg agccagccac gatcgacatt gatctggcta tcttgctgac aaaagcaaga
     1561 gaacatagcg ttgccttggt aggtccagcg gcggaggaac tctttgatcc ggttcctgaa
     1621 caggatctat ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg
     1681 gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca tttggtacag cgcagtaacc
     1741 ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct gccggcccag
     1801 tatcagcccg tcatacttga agctagacag gcttatcttg gacaagaaga agatcgcttg
     1861 gcctcgcgcg cagatcagtt ggaagaattt gtccattacg taaaaggcga gatcaccaag
     1921 gtagtcggca aataactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     1981 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     2041 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     2101 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     2161 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     2221 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     2281 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     2341 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     2401 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     2461 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     2521 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     2581 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     2641 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     2701 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     2761 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     2821 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     2881 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatcact ctgtggtctc
     2941 aa
//
