LOCUS       sequence-217654-        9452 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  P element vector for transposon insertion of Flag-tagged Drosophila 
            dhd into Drosophila genome.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9452)
  TITLE     Orr-Weaver plasmids
REFERENCE   2  (bases 1 to 9452)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: CaSpeR dhd 3X FLAG
FEATURES             Location/Qualifiers
     source          1..9452
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    join(332..886,887..917)
                     /label=P element 5' end
     rep_origin      complement(1152..1740)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1232..1251)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1911..2702,2703..2771))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2534..2553
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2772..2876)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2944..2962
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(3000..3022)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3122..3141
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     CDS             complement(join(3603..4217,4288..4419,4623..4938,5000..5654,
                     5729..6002,6404..6475))
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     primer_bind     6927..6945
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             8229..8294
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    join(8696..8897,8898..8928)
                     /label=P element 3' end
                     /note="P element 3' end"
ORIGIN
        1 gttcatttcg tctcaataga aattagtaat aaatatttgt atgtacaatt tatttgctcc
       61 aatatatttg tatatatttc cctcacagct atatttattc taatttaata ttatgacttt
      121 ttaaggtaat tttttgtgac ctgttcggag tgattagcgt tacaatttga actgaaagtg
      181 acatccagtg tttgttcctt gtgtagatgc atctcaaaaa aatggtgggc ataatagtgt
      241 tgtttatata tatcaaaaat aacaactata ataataagaa tacatttaat ttagaaaatg
      301 cttggatttc actggaacta gaattaattc ggctgctgct ctaaacgacg catttcgtac
      361 tccaaagtac gaattttttc cctcaagctc ttattttcat taaacaatga acaggaccta
      421 acgcacagtc acgttattgt ttacataaat gatttttttt actattcaaa cttactctgt
      481 ttgtgtactc ccactggtat agccttcttt tatcttttct ggttcaggct ctatcacttt
      541 actaggtacg gcatctgcgt tgagtcgcct ccttttaaat gtctgacctt ttgcaggtgc
      601 agccttccac tgcgaatcat taaagtgggt atcacaaatt tgggagtttt caccaaggct
      661 gcacccaagg ctctgctccc acaattttct cttaatagca cacttcggca cgtgaattaa
      721 ttttactcca gtcacagctt tgcagcaaaa tttgcaatat ttcatttttt tttattccac
      781 gtaagggtta atgttttcaa aaaaaaattc gtccgcacac aacctttcct ctcaacaagc
      841 aaacgtgcac tgaatttaag tgtatacttc ggtaagcttc ggctatcgac gggaccacct
      901 tatgttattt catcatgggc cagacccacg tagtccagcg gcagatcggc ggcggagaag
      961 ttaagcgtct ccaggatgac cttgcccgaa ctggggcacg tggtgttcga cgatgtgcag
     1021 ctaatttcgc ccggctccac gtccgcccat tggttaatca gcagaccctc gttggcgtaa
     1081 cggaaccatg agaggtacga caaccatttg aggtatactg gcaccgagcc cgagttcaag
     1141 aagaaggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     1201 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     1261 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     1321 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     1381 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     1441 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     1501 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     1561 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     1621 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     1681 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     1741 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     1801 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     1861 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     1921 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     1981 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     2041 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     2101 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     2161 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     2221 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     2281 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     2341 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     2401 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     2461 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     2521 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     2581 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     2641 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     2701 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     2761 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     2821 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     2881 tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat
     2941 aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac
     3001 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
     3061 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat
     3121 gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcaccga
     3181 atcgcgcgga actaacgaca gtcgctccaa ggtcgtcgaa caaaaggtga atgtgttgcg
     3241 gagagcgggt gggagacagc gaaagagcaa ctacgaaacg tggtgtggtg gaggtgaatt
     3301 atgaagaggg cgcgcgattt gaaaagtatg tatataaaaa atatatcccg gtgttttatg
     3361 tagcgataaa cgagtttttg atgtaaggta tgcaggtgtg taagtctttt ggttagaaga
     3421 caaatccaaa gtctacttgt ggggatgttc gaaggggaaa tacttgtatt ctataggtca
     3481 tatcttgttt ttattggcac aaatataatt acattagctt tttgaggggg caataaacag
     3541 taaacacgat ggtaataatg gtaaaaanaa aaaaacaagc agttatttcg gatatatgtc
     3601 ggctactcct tgcgtcgggc ccgaagtctt agagccagat atgcgagcac ccggaagctc
     3661 acgatgagaa tggccagacc cacgtagtcc agcggcagat cggcggcgga gaagttaagc
     3721 gtctccagga tgaccttgcc cgaactgggg cacgtggtgt tcgacgatgt gcagctaatt
     3781 tcgcccggct ccacgtccgc ccattggtta atcagcagac cctcgttggc gtaacggaac
     3841 catgagaggt acgacaacca tttgaggtat actggcaccg agcccgagtt caagaagaag
     3901 ccgccaaaga gcaggaatgg tatgataacc ggcggaccca cagacagcgc catcgaggtc
     3961 gaggagctgg cgcaggatat tagatatccg aaggacgttg acacattggc caccagagtg
     4021 accagcgcca ggcagttgaa gaagtgcagc actccggccc gcagtccgat catcggatag
     4081 gcaatcgccg tgaagaccag tggcactgtg agaaaaagcg gcaattcggc aatcgttttg
     4141 cccagaaagt atgtgtcaca gcgataaagt cgacttcggg cctccctcat aaaaactggc
     4201 agctctgagg tgaacaccta aatcgaatcg attcattaga aagttagtaa attattgaaa
     4261 tgcaaatgta ttctaaacat gacttacatt tatcgtggca aagacgtttt gaaaggtcat
     4321 gttggtcagg aagaggaaga tggctccgtt gatattcatc acacccactt gcgtgagttg
     4381 ttggcccaaa aagatgaggc caatcaagat ggcaaccatc tgcaaattaa aatgttactc
     4441 gcatctcatt aatattcgcg agttaaatga aatttattta tcttctgcaa aactataaac
     4501 tatacatctc attgaaaaaa actaagaagg gtgtggaatc aggcaattct atctaaaatc
     4561 tagcgaattt gtttccaaga attgtaagcg ttatatcatt tgtttccact ggaaccactc
     4621 accgttgtct gaataagtcg cacttttacg aggagtggtt ccttgagcac cgacagccag
     4681 gatcgccaca ggaccgcccg gaactgcatg aaccaggtgg ccttgtaggt gtacccattc
     4741 tccggctgct ccagtggctt ctccagattt ttggtggcca acaactgctc catatcccgg
     4801 gctactttgc taatggcaaa attgtcgcat atcttggcga tccgatcacg ggactcgatc
     4861 tcccgtccgg gcacaacggc caacacctgt acgtaaaagt ccgccggatt gtagttggta
     4921 ggacactggg cacccacgct ggataggagt tgagatgtta tgtaatacta gataccctta
     4981 ataaacacat cgaactcact aggaaaagaa gtcgacggct tcgctgggag tgcccaagaa
     5041 agctaccctg ccctcggcca tcagaaggat cttgtcaaag agctcaaaca gctcggaaga
     5101 cggctgatga atggtcagga tgacggtctt gcccttctgc gacagcttct tcagcacctg
     5161 gacgacgctg tgggcggtaa aggagtccag tccggaggtg ggctcatcgc agatcagaag
     5221 cggcggatcg gttagagcct cggaggcgaa tgccagacgc ttcctttctc cgccggacag
     5281 acctttcacc ctgccgggca caccgatgat cgtgtgctga catttgctga gcgaaagctc
     5341 ctggatcacc tgatccacgc gggccactcg ctgccgatag gtcagatgtc gtggcatccg
     5401 caccatggct tggaaaatca ggtgttccct ggccgttagg gagccgataa agaggtcatc
     5461 ctgctggaca taggcgcacc tggcctgcat ctccttggcg tccacaggtt ggccattgag
     5521 cagtcgcatc ccggatggcg atacttggat gccctgcggc gatcgaaagg caagggcatt
     5581 cagcagggtc gtctttccgg caccggaact gcccatcacg gccaaaagtt cgcccggata
     5641 ggccacgccg caaactgagt ttcaaattgg taattggacc ctttattaag atttcacaca
     5701 gatcagccga ctgcgaatag aaactcaccg ttcttgagca aatgtttcct gggcgccggt
     5761 atgtgtcgct cgttgcagaa tagtccgcgt gtccggttga ccagctgccg ccatccggag
     5821 cccggctgat tgaccgcccc aaagatgtcc atattgtgcc aggcataggt gaggttctcg
     5881 gctagttggc cgctccctga accggagtcc tccggcggac tgggtggccg gagcgtgccg
     5941 tagtttttgg cctgcccgaa gccctggtta atgcagctct gcgaagccgc tccgctgtca
     6001 ccctgcaatg ataggggatc tcaaatatca actacaagcg ttatgctcat ctaaccccga
     6061 acaaaaagta ccccgaagta tcctacgaag taggtttata cttttattta ttttttgtgc
     6121 atctaggatc agcttaaaat atctggttgt tatatttttt gtaaaaaaga atatagtcga
     6181 aaatgaatgc ctttagatgt cttgatcatg atatgatctc aaaaattgtc ttatatagcg
     6241 agaacagcta ccagaataat ctgtttcgtg tcactatttg tttgtgcaat tgcggtttgg
     6301 gatttttgtg ggtcgcagtt ctcacgccgc agacaatttg atgttgcaat cgcagttcct
     6361 atagatcaag tgaacttaag atgtatgcac atgtactact cacattgttc agatgctcgg
     6421 cagatgggtg tttgctgcct ccgcgaatta atagctcctg atcctcttgg cccattgccg
     6481 ggatttttca cactttcccc tgcttaccca cccaaaacca atcaccaccc caatcactca
     6541 aaaaacaaac aaaaataaga agcgagagga gttttggcac agcactttgt gtttaattga
     6601 tggcgtaaac cgcttggagc ttcgtcacga aaccgctgac aaaatgcaac tgaaggcgga
     6661 cattgacgct acgtaacgct acaaacggtg gcgaaagaga tagcggacgc agcggcgaaa
     6721 gagacggcga tatttctgtg gacagagaag gaggcaaaca gcgctgactt tgagtggaat
     6781 gtcattttga gtgagaggta atcgaaagaa cctggtacat caaataccct tggatcgaag
     6841 taaatttaaa actgatcaga taagttcaat gatatccagt gcagtaaaaa aaaaaaatgt
     6901 tttttttatc tactttccgc aaaaatgggt tttattaact tacatacata ctagaattcg
     6961 gtacccgccc ggggatcaga tccgcggccg cataggccac tagtcagttc gtccagcttg
     7021 ggtgctatta tcttgcaagg tccgcaccag tctgcataaa aatcaattac caccagcttg
     7081 tcctcggcga ggattagctg ctggtcaaga tcgtccttgt tccgcactgg gtacaccatg
     7141 actttaacta aattcccaac ggattgcaaa tcgaaacgaa acaaaaaaaa aaaaaatggg
     7201 aagagaagaa agaaaaaaaa aaggaaaatg ttagaaaatc ttgttttggg tgtaaaattc
     7261 acttgatttg ttttgttact tttgaatttt aatggagctc tgccctcgcc gagatatcag
     7321 cccgaaatga aaattgtagg gcggcaggcg ttcttttata cagttttcgc caaatatttt
     7381 gcggccttct tggattgaaa aaaaagtgtt ttcaaatgtc agcggcgcgg tgtgttgagg
     7441 tgagtttggc ttcgaaccta ccacggctat ggcaacactc gaggaaaata ttagtactat
     7501 gtcgtgtgac gccattattt tgccataatt acgataataa tattcatatc gatatcgata
     7561 gacctgccgt ggtgaataag tatgttcccc accccccaac acactcggtt ggctttccca
     7621 acgacctggc aaccctaaca cgcccgccat tttgcactct ccatgggccg ccattttgtt
     7681 tagcggccac gcgagaattc agcatgcaaa ttggcgggaa ttccactcta cacaccgcca
     7741 cttggagacc cctataaagt cgcccacgca gccgccattt caatcagttc acaatttgca
     7801 gcgaaagcgc caacagcccc atcacgtcaa gttctttaat ttaaaaaaaa gaagaaaaac
     7861 actttgctaa tcctcgcgac ataaaaatgg catccgtacg caccatgaac gactatcaca
     7921 agcgcatcga ggcggccgac gacaagctaa tcgtgctgga tttctatgcg acatggtgtg
     7981 gtccctgcaa ggaaatggag agcaccgtca aatcgctggc cagaaaatac tccagcaagg
     8041 cggtggtgct caagatcgat gtggacaaat tcgaggagct gacggagcgc tacaaggtgc
     8101 gcagcatgcc aacgtttgtc tttttgcgcc aaaatcgacg cctggcctcc tttgccggcg
     8161 ccgacgagca caagctgacc aacatgatgg ccaagctggt gaaggcgggc ggaagtggag
     8221 cggccgccga ctacaaagac catgacggtg attataaaga tcatgacatc gattacaagg
     8281 atgacgatga caagtaaggg cccgcagcaa tcctgcccag caattagcaa tcagcagcgc
     8341 atcttttttt tttttactta tttaactcat cttttaaaca tgttcgcctc atttgtgttc
     8401 gtttatgtat tcgatgttat gtgtatgctc atgtgatgtt tagcttgtaa gcgcgagatg
     8461 tgggtagcag gagatgcagt gcagccaaca gcagtgacca gatgatatat gctatgctac
     8521 tactactact tatatgctat gatttgtggc gcggaggcgt gtctgcgaca cataatcccg
     8581 cccattagct ttaagattca ggcactaaga agcaattcga tcaataaatt attgtaacca
     8641 ctctggacat ttaaatgtta actcgaggcc tcgaggtcga cctgcagcca agctttgcgt
     8701 actcgcaaat tattaaaaat aaaactttaa aaataatttc gtctaattaa tattatgagt
     8761 taattcaaac cccacggaca tgctaagggt taatcaacaa tcatatcgct gtctcactca
     8821 gactcaatac gacactcaga atactattcc tttcactcgc acttattgca agcatacgtt
     8881 aagtggatgt ctcttgccga cgggaccacc ttatgttatt tcatcatggt ctggccattc
     8941 tcatcgtgag cttccgggtg ctcgcatatc tggctctaag acttcgggcc cgacgcaagg
     9001 agtagccgac atatatccga aataactgct tgtttttttt ttttaccatt attaccatcg
     9061 tgtttactgt ttattgcccc ctcaaaaagc taatgtaatt atatttgtgc caataaaaac
     9121 aagatatgac ctatagaata caagtatttc cccttcgaac atccccacaa gtagactttg
     9181 gatttgtctt ctaaccaaaa gacttacaca cctgcatacc ttacatcaaa aactcgttta
     9241 tcgctacata aaacaccggg atatattttt tatatacata cttttcaaat cgcgcgccct
     9301 cttcataatt cacctccacc acaccacgtt tcgtagttgc tctttcgctg tctcccaccc
     9361 gctctccgca acacattcac cttttgttcg acgaccttgg agcgactgtc gttagttccg
     9421 cgcgattcgg ttcgctcaaa tggttccgag tg
//
