LOCUS       sequence-217663-        2876 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  for in vitro snRNA transcription.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2876)
  AUTHORS   Ghetti A, Company M, Abelson J
  TITLE     Specificity of Prp24 binding to RNA: a role for Prp24 in the dynamic
            interaction of U4 and U6 snRNAs.
  JOURNAL   RNA. 1995 Apr;1(2):132-45.
  PUBMED    7585243
REFERENCE   2  (bases 1 to 2876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA."; 
            date: "1995-04"; volume: "1(2)"; pages: "132-45"
COMMENT     Sequence Label: T7-U5 (long)
FEATURES             Location/Qualifiers
     source          1..2876
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     10..26
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10..26
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        41..59
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(286..303)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(286..302)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(295..317)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(511..530)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     630..652
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(690..708)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        776..880
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(881..949,950..1741)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1099..1118)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1912..2500
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2401..2420
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2654..2671
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2788..2809
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2824..2829,2830..2847,2848..2854)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    join(2862..2876,1..2)
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(2867..2876,1..13)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 aatttcacac aggaaacagc tatgacatga ttacgaattc taatacgact cactatagga
       61 agcagcttta cagatcaatg gcggagggag gtcaacatca agaactgtgg gccttttatt
      121 gcctatagaa cttataacga acatggttct tgccttttac cagaaccatc cgggtgttgt
      181 ctccatagaa acaggtaaag ctgtccgtta ctgtgggctt gccatatttt ttggaacttt
      241 tctgcccttt ttctcaatga gtaaggaggg cgtttaaagc ttggcactgg ccgtcgtttt
      301 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc
      361 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt
      421 gcgcagcctg aatggcgaat ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg
      481 tatttcacac cgcatatggt gcactctcag tacaatctgc tctgatgccg catagttaag
      541 ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc
      601 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc
      661 gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa
      721 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
      781 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
      841 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
      901 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
      961 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     1021 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     1081 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     1141 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     1201 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     1261 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     1321 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     1381 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     1441 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     1501 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     1561 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     1621 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     1681 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     1741 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     1801 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     1861 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     1921 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     1981 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     2041 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     2101 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     2161 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     2221 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     2281 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     2341 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     2401 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     2461 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     2521 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     2581 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     2641 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
     2701 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
     2761 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
     2821 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataac
//
