LOCUS       sequence-217678-        3283 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  for T7 transcription of wt actin pre-mRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3283)
  AUTHORS   Villa T, Guthrie C
  TITLE     The Isy1p component of the NineTeen complex interacts with the 
            ATPase Prp16p to regulate the fidelity of pre-mRNA splicing.
  JOURNAL   Genes Dev. 2005 Aug 15;19(16):1894-904. doi: 10.1101/gad.1336305.
  PUBMED    16103217
REFERENCE   2  (bases 1 to 3283)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1101/gad"; 
            journalName: "Genes Dev"; date: "2005-08-15- 15"; volume: "19"; 
            issue: "16"; pages: "1894-904"
COMMENT     Sequence Label: puC19 preACT1(WT)
FEATURES             Location/Qualifiers
     source          1..3283
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     12..31
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        12..30
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(628..645)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(628..644)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(637..659)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(1032..1050)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1118..1222
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1223..1291,1292..2083)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1441..1460)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2254..2842
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2743..2762
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2996..3013
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cttatcgaaa ttaatacgac tcactatagg gagaccggaa ttcgagctcg ccccttttag
       61 atttttcacg cttactgctt ttttcttccc aagatcgaaa atttactgaa ttaacaatgg
      121 attctggtat gttctagcgc ttgcaccatc ccatttaact gtaagaagaa ttgcacggtc
      181 ccaattgctc gagagatttc tcttttacct ttttttacta tttttcactc tcccataacc
      241 tcctatattg actgatctgt aataaccacg atattattgg aataaatagg ggcttgaaat
      301 ttggaaaaaa aaaaaaactg aaatattttc gtgataagtg atagtgatat tcttctttta
      361 tttgctactg tgtctcatgt actaacatcg attgcttcat tctttttgtt gctatattat
      421 atgtttagag gttgctgctt tggttattga taacggttct ggtatgtgta aagccggttt
      481 tgccggtgac gacgctcctc gtgctgtctt cccatctatc gtcggtagac caagacacca
      541 aggtatcatg gtcggtatgg gtcaaaaaga ctcctacgtt ggtgatgaag gggatcctct
      601 agagtcgacc tgcagcccaa gcttggcact ggccgtcgtt ttacaacgtc gtgactggga
      661 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
      721 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
      781 atggcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatatg
      841 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc
      901 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc
      961 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc
     1021 gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
     1081 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     1141 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     1201 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     1261 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     1321 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     1381 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     1441 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
     1501 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     1561 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     1621 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     1681 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     1741 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     1801 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     1861 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     1921 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     1981 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     2041 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     2101 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     2161 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     2221 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     2281 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     2341 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     2401 ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     2461 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     2521 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     2581 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     2641 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     2701 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     2761 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     2821 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     2881 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     2941 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     3001 gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg
     3061 cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg cgctacgtga
     3121 ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg
     3181 tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca
     3241 gaggttttca ccgtcatcac cgaaacgcgc gaggcccagc tgg
//
