LOCUS       sequence-217714-       10722 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression vector for NLS-iRFP-NLS, JNK KTR-mCherry, and mKO-MK2 in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10722)
  AUTHORS   Miura H, Kondo Y, Matsuda M, Aoki K
  TITLE     Cell-to-Cell Heterogeneity in p38-Mediated Cross-Inhibition of JNK 
            Causes Stochastic Cell Death.
  JOURNAL   Cell Rep. 2018 Sep 4;24(10):2658-2668. doi: 
            10.1016/j.celrep.2018.08.020.
  PUBMED    30184500
REFERENCE   2  (bases 1 to 10722)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2018.08"; journalName: "Cell Rep"; date: 
            "2018-09-4- 4"; volume: "24"; issue: "10"; pages: "2658-2668"
COMMENT     Sequence Label: pNJP
FEATURES             Location/Qualifiers
     source          1..10722
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   674..708
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        1011..1390
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1393..1668
                     /label=chicken beta-actin promoter
     intron          1669..2677
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2601..2623
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     CDS             2774..2794
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3776..3796
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3806..3862
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4052..4756
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHFTRGMDELY"
     primer_bind     complement(4197..4215)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4465..4484
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             4766..4822
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4823..5476
                     /codon_start=1
                     /product="humanized monomeric Kusabira-Orange fluorescent 
                     protein"
                     /label=hmKO
                     /note="mammalian codon-optimized"
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAKGGPMPFAFDLVSHVFCYGHRPFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQFKTTYKAAKKILKMPGSHYISHRLVRKTEGNITELGEDAVAHS"
     misc_feature    6787..7335
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6931..6948)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7158..7177
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     complement(7806..7823)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7812..8036
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(8083..8099)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8107..8123
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8131..8137,8138..8155,8156..8161))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8176..8197
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     repeat_region   8431..8493
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(8534..8554)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(8534..8552)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8573..8589)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8597..8613
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8621..8627,8628..8645,8646..8651))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8666..8687
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8804..8821)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8975..9563)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9055..9074)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9734..10525,10526..10594))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10357..10376
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10595..10699)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggggcgcgc
      661 cattctagat taaccctaga aagatagtct gcgtaaaatt gacgcatgca ttcttgaaat
      721 attgctctct ctttctaaat agcgcgaatc cgtcgctgtg catttaggac atctcagtcg
      781 ccgcttggag ctcccgtgag gcgtgcttgt caatgcggta agtgtcactg attttgaact
      841 ataacgaccg cgtgagtcaa aatgacgcat gattatcttt tacgtgactt ttaagattta
      901 actcatacga taattatatt gttatttcat gttctactta cgtgataact tattatatat
      961 atattttctt gttatagata tcaactagaa tgctagcatg ggcccatctc gacattgatt
     1021 attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
     1081 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
     1141 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
     1201 acgtcaatgg gtggactatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
     1261 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
     1321 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
     1381 tattaccatg ggtcgaggtg agccccacgt tctgcttcac tctccccatc tcccccccct
     1441 ccccaccccc aattttgtat ttatttattt tttaattatt ttgtgcagcg atgggggcgg
     1501 gggggggggg ggcgcgcgcc aggcggggcg gggcggggcg aggggcgggg cggggcgagg
     1561 cggagaggtg cggcggcagc caatcagagc ggcgcgctcc gaaagtttcc ttttatggcg
     1621 aggcggcggc ggcggcggcc ctataaaaag cgaagcgcgc ggcgggcggg agtcgctgcg
     1681 ttgccttcgc cccgtgcccc gctccgcgcc gcctcgcgcc gcccgccccg gctctgactg
     1741 accgcgttac tcccacaggt gagcgggcgg gacggccctt ctcctccggg ctgtaattag
     1801 cgcttggttt aatgacggct cgtttctttt ctgtggctgc gtgaaagcct taaagggctc
     1861 cgggagggcc ctttgtgcgg gggggagcgg ctcggggggt gcgtgcgtgt gtgtgtgcgt
     1921 ggggagcgcc gcgtgcggcc cgcgctgccc ggcggctgtg agcgctgcgg gcgcggcgcg
     1981 gggctttgtg cgctccgcgt gtgcgcgagg ggagcgcggc cgggggcggt gccccgcggt
     2041 gcgggggggc tgcgagggga acaaaggctg cgtgcggggt gtgtgcgtgg gggggtgagc
     2101 agggggtgtg ggcgcggcgg tcgggctgta acccccccct gcacccccct ccccgagttg
     2161 ctgagcacgg cccggcttcg ggtgcggggc tccgtgcggg gcgtggcgcg gggctcgccg
     2221 tgccgggcgg ggggtggcgg caggtggggg tgccgggcgg ggcggggccg cctcgggccg
     2281 gggagggctc gggggagggg cgcggcggcc ccggagcgcc ggcggctgtc gaggcgcggc
     2341 gagccgcagc cattgccttt tatggtaatc gtgcgagagg gcgcagggac ttcctttgtc
     2401 ccaaatctgg cggagccgaa atctgggagg cgccgccgca ccccctctag cgggcgcggg
     2461 cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga gggccttcgt gcgtcgccgc
     2521 gccgccgtcc ccttctccat ctccagcctc ggggctgccg cagggggacg gctgccttcg
     2581 ggggggacgg ggcagggcgg ggttcggctt ctggcgtgtg accggcggct ctagagcctc
     2641 tgctaaccat gttcatgcct tcttcttttt cctacagctc ctgggcaacg tgctggttgt
     2701 tgtgctgtct catcattttg gcaaagaatt gggatccgaa tttcatcatt ttggcaaaga
     2761 attcaccatg gcccctaaga agaagcgtaa ggtgatggcc gaagggagcg tggcaagaca
     2821 gcccgatctg ctgacatgcg acgacgaacc aatccacatt ccaggagcta tccagcctca
     2881 tggactgctg ctggctctgg ccgctgacat gactatcgtg gcaggctctg ataacctgcc
     2941 agagctgacc gggctggcta tcggagcact gattggccgg tccgcagccg acgtgttcga
     3001 ttctgagact cacaatagac tgaccatcgc cctggctgaa cctggggctg cagtgggagc
     3061 cccaattacc gtcggcttca caatgcgaaa ggacgctggc tttatcgggt cctggcaccg
     3121 ccatgatcag ctgatttttc tggagctgga accacctcag agagacgtgg cagagccaca
     3181 ggctttcttt cggagaacaa acagcgccat caggcgcctg caggccgctg agactctgga
     3241 atccgcttgc gcagccgctg cacaggaagt gaggaagatc accgggttcg atcgcgtcat
     3301 gatctacaga ttcgcctcag actttagcgg agaagtgatc gcagaagatc gctgtgccga
     3361 ggtcgaaagt aaactgggcc tgcattaccc agcctcaacc gtgcctgcac aggcacgacg
     3421 gctgtataca atcaaccccg tgcggatcat tcccgacatt aattacagac cagtgcccgt
     3481 cacacctgac ctgaacccag tgactgggag gcccatcgat ctgagcttcg ccattctgcg
     3541 cagcgtgtcc cctgtccacc tggagtttat gaggaatatc ggaatgcatg gcaccatgtc
     3601 tatcagtatt ctgagaggcg aaaggctgtg ggggctgatc gtgtgccacc atcggacacc
     3661 ttactatgtg gacctggacg gcaggcaggc ttgtgaactg gtggctcagg tgctggcttg
     3721 gcagattggc gtgatgaagg aggggcatgg caccggcagc accggcagcg gcagccctaa
     3781 gaagaagcgt aaggtgggga gcggggctac caacttctcc ctcctcaagc aggcagggga
     3841 cgtcgaggaa aatcctggac ccatgagtaa ccctaagatc ctaaaacaga gcatgacctt
     3901 gaacctggcc gacccggtgg gcagtctgaa gccgcacctc cgcgccaagc gctcggccgg
     3961 tctcaacgaa gacgagtcgc catctaagaa gctggcgtcg ccggaggtgt cgtcaaggct
     4021 ggagcgcctg accctccagt ccagcggccg catggtgagc aagggcgagg aggataacat
     4081 ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca
     4141 cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa
     4201 gctgaaggtg accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccctcagtt
     4261 catgtacggc tccaaggcct acgtgaagca ccccgccgac atccccgact acttgaagct
     4321 gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt
     4381 gaccgtgacc caggactcct ccctgcagga cggcgagttc atctacaagg tgaagctgcg
     4441 cggcaccaac ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc
     4501 ctcctccgag cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agcagaggct
     4561 gaagctgaag gacggcggcc actacgacgc tgaggtcaag accacctaca aggccaagaa
     4621 gcccgtgcag ctgcccggcg cctacaacgt caacatcaag ttggacatca cctcccacaa
     4681 cgaggactac accatcgtgg aacagtacga acgcgccgag ggccgccact tcactcgcgg
     4741 catggacgag ctgtacgggt caggggccac caacttctct cttctgaaac aagccggcga
     4801 cgtcgaggaa aaccccggac ctatggtgag cgtgatcaag cccgagatga agatgaggta
     4861 ctacatggac ggctccgtca atgggcatga gttcacaatc gagggtgagg gcacaggcag
     4921 accttacgag ggacatcagg agatgacact gcgcgtcaca atggccaagg gcgggccaat
     4981 gcctttcgcc ttcgacctgg tgtcccacgt gttctgttac ggccacagac cttttactaa
     5041 atatccagaa gagatcccag actatttcaa gcaggccttt cctgagggcc tgtcctggga
     5101 gaggtccctg gagttcgagg acggcggctc cgcctccgtg agcgcccaca tcagcctgag
     5161 gggcaacacc ttctaccaca agtccaagtt caccggcgtg aacttccccg ccgacggccc
     5221 catcatgcag aaccagagcg tggactggga gccctccacc gagaagatca ccgccagcga
     5281 cggcgtgctg aagggcgacg tgaccatgta cctgaagctg gagggcggcg gcaaccacaa
     5341 gtgccagttc aagaccacct acaaggccgc caagaagatc ctgaagatgc ccggcagcca
     5401 ctacatcagc cacaggctgg tgaggaagac cgagggcaac atcaccgagc tgggggagga
     5461 cgccgtggcc cactccctcg agatgctgtc caactcccag ggccagagcc cgccggtgcc
     5521 gttccccgcc ccggccccgc cgccgcagcc ccccacccct gccctgccgc accccccggc
     5581 gcagccgccg ccgccgcccc cgcagcagtt cccgcagttc cacgtcaagt ccggcctgca
     5641 gatcaagaag aacgccatca tcgatgacta caaggtcacc agccaggtcc tggggctggg
     5701 catcaacggc aaagttttgc agatcttcaa caagaggacc caggagaaat tcgccctcaa
     5761 aatgcttcag gactgcccca aggcccgcag ggaggtggag ctgcactggc gggcctccca
     5821 gtgcccgcac atcgtacgga tcgtggatgt gtacgagaat ctgtacgcag ggaggaagtg
     5881 cctgctgatt gtcatggaat gtttggacgg tggagaactc tttagccgaa tccaggatcg
     5941 aggagaccag gcattcacag aaagagaagc atccgaaatc atgaagagca tcggtgaggc
     6001 catccagtat ctgcattcaa tcaacattgc ccatcgggat gtcaagcctg agaatctctt
     6061 atacacctcc aaaaggccca acgccatcct gaaactcact gactttggct ttgccaagga
     6121 aaccaccagc cacaactctt tgaccactcc ttgttataca ccgtactatg tggctccaga
     6181 agtgctgggt ccagagaagt atgacaagtc ctgtgacatg tggtccctgg gtgtcatcat
     6241 gtacatcctg ctgtgtgggt atcccccctt ctactccaac cacggccttg ccatctctcc
     6301 gggcatgaag actcgcatcc gaatgggcca gtatgaattt cccaacccag aatggtcaga
     6361 agtatcagag gaagtgaaga tgctcattcg gaatctgctg aaaacagagc ccacccagag
     6421 aatgaccatc accgagttta tgaaccaccc ttggatcatg caatcaacaa aggtccctca
     6481 aaccccactg cacaccagcc gggtcctgaa ggaggacaag gagcggtggg aggatgtcaa
     6541 ggaggagatg accagtgcct tggccacaat gcgcgttgac tacgagcaga tcaagataaa
     6601 aaagattgaa gatgcatcca accctctgct gctgaagagg cggaagaaag ctcgggccct
     6661 ggaggctgcg gctctggccc actgaggcgg ccgctctaga gtcgacgggc cgcggtaaca
     6721 attgttaact aacttaagct agcaacggtt tccctctagc gggatcaatt ccgccccccc
     6781 cccctaacgt tactggccga agccgcttgg aataaggccg gtgtgcgttt gtctatatgt
     6841 tattttccac catattgccg tcttttggca atgtgagggc ccggaaacct ggccctgtct
     6901 tcttgacgag cattcctagg ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga
     6961 atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag acaaacaacg tctgtagcga
     7021 ccctttgcag gcagcggaac cccccacctg gcgacaggtg cctctgcggc caaaagccac
     7081 gtgtataaga tacacctgca aaggcggcac aaccccagtg ccacgttgtg agttggatag
     7141 ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg aaggatgccc
     7201 agaaggtacc ccattgtatg ggatctgatc tggggcctcg gtgcacatgc tttacatgtg
     7261 tttagtcgag gttaaaaaac gtctaggccc cccgaaccac ggggacgtgg ttttcctttg
     7321 aaaaacacga taataccatg gtcatgaaaa catttaacat ttctcaacaa gatctagaat
     7381 tagtagaagt agcgacagag aagattacaa tgctttatga ggataataaa catcatgtgg
     7441 gagcggcaat tcgtacgaaa acaggagaaa tcatttcggc agtacatatt gaagcgtata
     7501 taggacgagt aactgtttgt gcagaagcca ttgcgattgg tagtgcagtt tcgaatggac
     7561 aaaaggattt tgacacgatt gtagctgtta gacaccctta ttctgacgaa gtagatagaa
     7621 gtattcgagt ggtaagtcct tgtggtatgt gtagggagtt gatttcagac tatgcaccag
     7681 attgttttgt gttaatagaa atgaatggca agttagtcaa aactacgatt gaagaactca
     7741 ttccactcaa atatacccga aattaaaagt cgagatgcat cgatgatcta gagctcgctg
     7801 atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc
     7861 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc
     7921 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa
     7981 gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc
     8041 tgaggcggaa agaacctgca gcccaagctt ggcgtaatca tggtcatagc tgtttcctgt
     8101 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     8161 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     8221 tttccagtcg ggaaacctgt cgtgccagcg gatcttaaaa gttttgttac tttatagaag
     8281 aaattttgag tttttgtttt tttttaataa ataaataaac ataaataaat tgtttgttga
     8341 atttattatt agtatgtaag tgtaaatata ataaaactta atatctattc aaattaataa
     8401 ataaacctcg atatacagac cgataaaaca catgcgtcaa ttttacgcat gattatcttt
     8461 aacgtacgtc acaatatgat tatctttcta gggttaatct agtatacgcg tttaattaat
     8521 ccagcttttg ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca tggtcatagc
     8581 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     8641 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     8701 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     8761 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     8821 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     8881 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     8941 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     9001 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     9061 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     9121 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     9181 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     9241 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     9301 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     9361 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
     9421 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     9481 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     9541 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     9601 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     9661 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     9721 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     9781 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     9841 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     9901 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     9961 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
    10021 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
    10081 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
    10141 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
    10201 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
    10261 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
    10321 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
    10381 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
    10441 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
    10501 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
    10561 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
    10621 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
    10681 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc ac
//
